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조선대학교 치의학연구원> Oral Biology Research (OBR)> Characterization of hemin-binding of oral streptococci

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Characterization of hemin-binding of oral streptococci

Eun Jeong Kim , Si Young Lee
  • : 조선대학교 치의학연구원
  • : Oral Biology Research (OBR) 46권3호
  • : 연속간행물
  • : 2022년 09월
  • : 99-105(7pages)
Oral Biology Research (OBR)

DOI


목차

Introduction
Materials and Methods
Results
Discussion
Funding
Conflicts of Interest
ORCID
References

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Certain pathogenic bacteria obtain iron for growth through heme or heme compounds. Streptococci are gram-positive bacteria that reside in the mouth and require iron for growth. However, the iron accumulation mechanism in streptococci remains unknown. Therefore, this study investigated the hemin-binding properties of streptococci to confirm the potential of oral streptococci to obtain iron through heme compounds. The hemin-binding ability of streptococci was evaluated by incubation with hemin and subsequent measurement of the optical density of the supernatant to determine the amount of hemin bound by the bacteria using the hemin concentration standard curve. The hemin-binding of streptococci was proportional to the concentration of bacteria (Streptococcus gordonii) and hemin added. However, when a high hemin concentration of up to 60 μg/mL was added, the amount of hemin bound by S. gordonii remained almost unchanged. The incubation temperature had no significant effect on hemin-binding, but pretreatment with trypsin and protease decreased hemin-binding. Additionally, we experimentally confirmed that S. gordonii could use hemin for growth. This study demonstrated that streptococci possess hemin-binding sites and can acquire iron through hemin.

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간행물정보

  • : 의약학분야  > 기초치의학
  • : KCI등재
  • :
  • : 계간
  • : 2508-2558
  • : 2508-2566
  • : 학술지
  • : 연속간행물
  • : 1978-2022
  • : 1503


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1Smad3 deficiency accelerates tongue wound healing via epithelial cell-extracellular matrix interactions

저자 : Chieko Taguchi , Fuyuki Sato , Chen Wang , Shigeru Nakamura , Kosuke Oikawa , Ujjal Kumar Bhawal , Hiroyuki Okada , Kazumune Arikawa

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 46권 4호 발행 연도 : 2022 페이지 : pp. 141-149 (9 pages)

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Aberrant wound closure occurs in a broad range of wounds and scars, and the altered regulation of transcription factors in wound areas can account for both of those conditions. This study aimed to explore the function of the transcription factor Smad3 in wound healing using a tongue wound model in Smad3 knockout (Smad3-/-) mice and with Smad3 small interfering RNA (siRNA) transfected human gingival fibroblasts (HGFs). Smad3-/- mice were used to examine the extent of repair in tongue wounds. Cell migration was evaluated in HGFs using wound healing assays. The mRNA expression levels of Sox2, E-cadherin, fibronectin, and vimentin were examined in HGFs using reverse transcription-quantitative polymerase chain reaction. Histopathological analysis of wound closure in Smad3-/- mice showed rapid re-epithelialization and remodeling in tongue wound repair compared with Smad3+/+ mice. Increased numbers of neutrophils were identified in the wounds of Smad3-/- mice. Sox2 and phospho-E-cadherin expression levels were increased in Smad3-/- mice. Smad3 knockdown by siRNA increased cell migration of HGFs. In addition, Sox2, E-cadherin, fibronectin, and vimentin mRNA levels were significantly increased in Smad3 siRNA-transfected HGFs compared with controls. Collectively, these findings demonstrate that a Smad3 deficiency can expedite wound healing and increase immune reactions and extracellular matrix formation after tongue injuries, boosting recovery through Sox2 and E-cadherin. Consequently, Smad3 inhibition would help stimulate tongue wound healing.

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2Case series of maxillary anterior bone augmentation with a novel biphasic calcium phosphate: a clinical and radiographic pilot study

저자 : Jae-hong Lee , Eun-hee Jung , Hyun-wook An , Jae-seung Im , Dong-won Lee , Jeong-ho Yun

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 46권 4호 발행 연도 : 2022 페이지 : pp. 150-157 (8 pages)

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The purpose of this pilot case series was to evaluate the clinical and radiographic outcomes of employing newly developed alloplastic biphasic calcium phosphate (BCP) in guided bone regeneration (GBR) for maxillary anterior peri-implant defects. Six peri-implant dehiscence defects were grafted with BCP. For all included treatment sites, clinical (defect width [DW] and defect height [DH]), radiographic (horizontal hard tissue thickness [HT]), patient discomfort, and early wound healing outcomes were evaluated. At reentry surgery, all surgical sites indicated a change in DW, DH, HT0, HT1, HT2, and HT3 from 4.08±1.39 mm to 1.13±1.76 mm, 3.57±1.42 mm to 0.58±1.09 mm, 2.18±0.66 mm to 1.50±0.53 mm, 2.11±0.57 mm to 1.73±0.28 mm, 2.22±0.54 mm to 1.75±0.26 mm, and 2.63±0.87 mm to 1.83±0.46 mm, respectively. Significant differences were discovered between paired DW and DH (p<0.05), whereas radiographic parameters had no significant differences. The severity of pain and swelling was 4.8±1.9 and 6.5±1.9, respectively, and the duration of pain and swelling was 4.5±3.8 and 5.8±2.9 days, respectively, according to early postoperative discomfort assessment. No adverse reactions occurred at any treatment site. In terms of clinical and radiographic outcomes, the newly developed BCP is acceptable biocompatible and suitable for the GBR of maxillary anterior peri-implant dehiscence defects within the limitations of this study.

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3Effect of alar base cinch suturing on preventing nasal deformation during orthognathic surgery with Le Fort I osteotomy

저자 : Kyung-min Lee , Jae-woo Jang , Young-wook Park

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 46권 4호 발행 연도 : 2022 페이지 : pp. 158-164 (7 pages)

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This retrospective study assessed changes in interalar width (IAW) resulting from the alar base cinch suturing in 17 patients who underwent Le Fort I osteotomy. After maxillary fixation, alar base cinch suturing was performed through levator labii superioris alaque nasi muscle, alar part of nasalis muscle, and fibroareolar tissues. The IAW was measured in all patients using a vernier caliper preoperatively (T0), one day postoperatively (T1), one week postoperatively (T2), one month postoperatively (T3), and six months postoperatively (T4). Results showed that the IAW at T1 was significantly narrower than at T0, and the measurement was not changed significantly after that. Therefore, effective alar base cinch suturing prevents horizontal nasal soft tissue from widening.

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4Effects of cimifugin on cell growth inhibition and cell apoptosis induction in fadu human pharyngeal squamous cell carcinoma

저자 : Jong-hyun Park , Do Kyung Kim

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 46권 4호 발행 연도 : 2022 페이지 : pp. 165-170 (6 pages)

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Cimifugin is an important component of chromones in the dry roots of Saposhnikovia divaricata and is used in treating inflammatory diseases. However, the effect of cimifugin on cancer is unclear. The aim of this study was to investigate the effects of cimifugin on cell apoptosis induction in FaDu human pharyngeal carcinoma cells using MTT assay, live/dead cell assay, and western blot. Cimifugininduced FaDu cell death in a dose-dependent manner. Cimifugin-induced apoptosis in FaDu cells was mediated by the expression of Fas and activation of caspase-8, caspase-3, and poly (ADP-ribose) polymerase (PARP). Western blot results showed the downregulation of Bcl-2 and Bcl-xL but the upregulation of Bad and Bax by cimifugin in FaDu cells. These results suggest that cimifugin inhibits cell survival and induces apoptotic cell death in FaDu human pharyngeal carcinoma cells via both the death receptor-mediated extrinsic apoptotic pathway and the mitochondria-mediated intrinsic apoptotic pathway.

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5In vitro and in vivo analyses of the biocompatibility of sintered TCP-HA composite screws

저자 : Young-gon Sun , Myeong-seop Lim , Young-joon Kim , Jeong-in Choi

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 46권 4호 발행 연도 : 2022 페이지 : pp. 171-182 (12 pages)

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Biphasic calcium phosphate (BCP), which is a β-tricalcium phosphate (TCP) and hydroxyapatite (HA) composite, is used mainly as a bone graft material. However, it has recently been used for orthopedic, oral, and maxillofacial surgeries. The use of BCP to produce an absorbable screw and plate to reconstruct fractures has been suggested. Nevertheless, further research on using BCP in the surgical field will be needed. In particular, studies on the physical properties of BCP and in vivo analysis are essential. In this study, TCP and HA were mixed at a ratio of 7:3 and heat-treated to form BCP. Scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, and an MTT assay were conducted to evaluate the surface characteristics and physical and biological properties of each sample. In addition, BCP was used to produce a screw to be implanted into the mandible of beagles. An analysis of the physical properties of BCP confirmed the intermediate properties between TCP and HA. The cell viability was better with BCP than TCP, and significant differences were observed on days 1 and 3. Histological analysis at eight weeks after inserting the BCP screw into the mandible of beagles revealed osseointegration between the BCP screw and bone. The results showed that BCP had better mechanical properties than TCP and excellent biocompatibility, highlighting its potential clinical use. In addition, according to its processing, BCP may be used to produce screws and plates for fracture repair or as membranes and space-modeling tents for guided bone regeneration.

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6The etiology and possible molecular mechanisms underlying calcium channel blocker-induced gingival enlargement: a narrative review

저자 : Seul Lee , Hyung-keun You

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 46권 4호 발행 연도 : 2022 페이지 : pp. 183-188 (6 pages)

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Gingival enlargement, defined as the abnormal proliferation of gingival tissue, can be caused by exposure to poor oral hygiene, periodontal diseases, or systemic drugs such as antihypertensive, immunosuppressive, and anticonvulsant agents. Although numerous clinical studies have examined drug-induced gingival enlargement, there is limited evidence on the molecular mechanisms underlying this disease. Therefore, the current study aims to summarize the available literature on the effects of systemic drugs such as antihypertensive agents and calcium channel blockers used for the treatment of cardiac diseases on gingival enlargement expression, and to discuss possible molecular mechanisms underlying drug-induced gingival enlargement.

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7Headache disorders in dental practice

저자 : Jin-kyu Kang , Jin-han Lee

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 46권 4호 발행 연도 : 2022 페이지 : pp. 189-194 (6 pages)

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Headache is a common pain disorder. Primary headaches constitute most headache disorders, with migraine and tension headaches being the most common. In contrast, secondary headaches are rare and accompanied by the underlying disease. Headaches due to dental diseases and temporomandibular disorders have also been reported. Patients complaining of headaches are often observed in dental clinics, so distinguishing each headache is crucial for specific management. Therefore, dentists should be familiar with the diagnostic criteria for primary and secondary headaches. Moreover, some secondary headaches can be life-threatening or red flags of serious diseases such as cerebral hemorrhage and brain tumor, so it is necessary to differentiate them and take emergency management or refer the patient to the appropriate department.

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8Diagnosis and treatment of maxillary molar with abnormality

저자 : Kkot-byeol Bae , Bin-na Lee , Hoon-sang Chang , In-nam Hwang , Won-mann Oh , Yun-chan Hwang

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 46권 4호 발행 연도 : 2022 페이지 : pp. 195-199 (5 pages)

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Endodontic treatment of an abnormally shaped tooth is complicated by irregular tooth morphology. Furthermore, root canal fusions are known to occur in abnormal teeth. The placement of canal orifices is unusual and due to their depth, they are sometimes invisible to the naked eye. As a result of the difficulty in instrumentations and obturation, root canal treatment of teeth with an abnormality is difficult. When performing root canal treatment on abnormal teeth, complex anatomic variations of the root canal system should be investigated as thoroughly as possible. Cone-beam computed tomography is an effective method for assessing the morphology of the root canal by visualizing the canal of the tooth. The case reports that follow each describe a case of root canal treatment in a maxillary molar with an abnormality.

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9A case report of nonsurgical treatment of maxillary anterior peri-implantitis

저자 : Min-cheol Yang , Seok-hwan Jeong , Kyu-man Lee , Byung-ock Kim

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 46권 4호 발행 연도 : 2022 페이지 : pp. 200-204 (5 pages)

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Peri-implantitis is defined as an inflammatory reaction associated with bone loss beyond the initial biologic bone remodeling around a functional implant. This study aims to report the clinical and radiographic outcomes of nonsurgical peri-implantitis treatment in the maxillary anterior area. Subgingival irrigation with normal saline and antiseptics, systemic antibiotics, laser decontamination, and biofilm decontamination approach in department of periodontics and water irrigation as home care were conducted. Nonsurgical treatment of peri-implantitis resulted in healing, such as improvements in bleeding tendency, pus discharge, bad odor, and gingival recession, and resulted in a little bit bone changes around implants with severe bone resorption and shallow pocket probing depth over a 5-yr maintenance period. We confirmed that nonsurgical therapy with regular follow-up maintained peri-implantitis with an initial shallow pocket probing depth in the maxillary anterior area.

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10Removable partial denture restoration using implant-supported surveyed crowns

저자 : Yu-ri Heo

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 46권 4호 발행 연도 : 2022 페이지 : pp. 205-209 (5 pages)

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Conventional dentures and implant-fixed prostheses are the treatment of choice for patients with multiple missing teeth. Implantassisted removable partial dentures (IARPD) represent a viable treatment option that combines these two treatment modalities to provide greater retention and stability compared to dentures alone. IARPDs also offer enhanced esthetic characteristics in patients with severe bone loss, and treatment outcomes can be improved further through careful diagnosis taking the degree of bone loss and the patient's facial features into consideration. Accurate implant placement that is compatible with the partial denture design and the opposing dentition is also crucial for success. The current clinical series includes patients that underwent partial denture treatment using implant-supported surveyed crowns, and presented satisfactory functional and esthetic treatment outcomes.

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1Characterization of hemin-binding of oral streptococci

저자 : Eun Jeong Kim , Si Young Lee

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 46권 3호 발행 연도 : 2022 페이지 : pp. 99-105 (7 pages)

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Certain pathogenic bacteria obtain iron for growth through heme or heme compounds. Streptococci are gram-positive bacteria that reside in the mouth and require iron for growth. However, the iron accumulation mechanism in streptococci remains unknown. Therefore, this study investigated the hemin-binding properties of streptococci to confirm the potential of oral streptococci to obtain iron through heme compounds. The hemin-binding ability of streptococci was evaluated by incubation with hemin and subsequent measurement of the optical density of the supernatant to determine the amount of hemin bound by the bacteria using the hemin concentration standard curve. The hemin-binding of streptococci was proportional to the concentration of bacteria (Streptococcus gordonii) and hemin added. However, when a high hemin concentration of up to 60 μg/mL was added, the amount of hemin bound by S. gordonii remained almost unchanged. The incubation temperature had no significant effect on hemin-binding, but pretreatment with trypsin and protease decreased hemin-binding. Additionally, we experimentally confirmed that S. gordonii could use hemin for growth. This study demonstrated that streptococci possess hemin-binding sites and can acquire iron through hemin.

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2Effect of cetylpyridinium chloride on dental unit waterline biofilms in-vitro

저자 : Si Young Lee , Hye Young Yoon

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 46권 3호 발행 연도 : 2022 페이지 : pp. 106-110 (5 pages)

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Various disinfectants are used to remove biofilms formed in dental unit waterlines (DUWLs). This study aimed to assess the antibiofilm effects of cetylpyridinium chloride (CPC) on DUWL biofilms formed in a CDC Biofilm Reactor model and evaluate the feasibility of CPC as a novel DUWL disinfectant. For experiments, CPC was used at concentrations of 0.003125% to 0.00039%. Well-established DUWL disinfectants, such as 0.5% sodium hypochlorite (NaOCl), 0.12% chlorhexidine (CHX), and a mixture of 0.12% CHX and 12% ethanol, were used as standards. DUWL biofilms formed in the CDC Biofilm Reactor model were exposed to the disinfecting agents for 15 min. The number of living bacteria in the biofilms before and after exposure to chemicals were compared, and the biofilm reduction rate was calculated. The biofilm reduction rate of CPC at a concentration higher than 0.001562% was greater than that of all other conventional disinfecting agents. These findings suggest that CPC can be used for effective removal of DUWL biofilms.

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3Evaluation of antioxidant and anticancer activities of fermented pericarp extract of Camellia japonica L. using Aspergillus oryzae in oral cancer

저자 : Eugene Cho , Hyoun Woo Kim , Da Hye Jeong , Seokmyon Lee , Deuk-sil Oh , Jin Kim

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 46권 3호 발행 연도 : 2022 페이지 : pp. 111-118 (8 pages)

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This study aimed to investigate the antioxidant and anticancer activities of the fermented extract of the dried pericarp of Camellia japonica L. (CJ). The antioxidant activity of the extract after fermentation by Aspergillus oryzae (A. oryzae) at different doses, times, and temperatures was slightly decreased compared with that of the unfermented extract. The total polyphenol content decreased significantly as fermentation time was increased at 30℃, but there was no significant change when the fermentation was performed for 72 hr at different temperatures. The total flavonoid content was not significantly changed by fermentation conditions. Evaluation of the anticancer activity of the A. oryzae-fermented extract revealed that there was no effect on the viability of immortalized 293T cells treated with the extract for 48 hr, but the viability of YD-8 and YD-10B oral cancer cells was significantly reduced. In addition, there was a significant increase in the expression of cleaved PARP and caspase 3, and in apoptotic (annexin V/PI-stained) cells. These results suggest that the water extract of dried pericarp of CJ has no anticancer activity, but gained anticancer activity following fermentation by A. oryzae; furthermore, the fermented extract may be a potential treatment for oral cancer.

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4Vertical ridge augmentation for implant placement using two types of titanium mesh on edentulous atrophic maxilla: clinical results of three patients

저자 : Ho-jin Yun , Min-young Goo , Kyeong-ok Lim , Won-pyo Lee , Byung-ock Kim , Sang-joun Yu

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 46권 3호 발행 연도 : 2022 페이지 : pp. 119-127 (9 pages)

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This study aimed to compare the vertical and horizontal bone augmentation achieved when using different types of titanium mesh (Ti-mesh) on edentulous atrophic maxilla. Surgical intervention was performed in the maxillary molar area of three patients. One patient received A type Ti-mesh (aTM; pore size: 0.9 mm, thickness: 0.1 mm), and placement of one implant was carried out after 6 months. The remaining two patients received B type Ti-mesh (bTM; pore size: 1.7 mm, 3.37 mm; thickness: 0.3 mm;), and 4 implants were placed after 6 months. The findings showed that both types of Ti-mesh were associated with good feasibility and predictability with regard to vertical and horizontal bone augmentation. Pore size did not affect the amount of bone regenerated for implant placement. However, bTM was associated with several advantages, including decreased operation time and increased convenience during guided bone regeneration surgery.

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5Impaction of permanent canine caused by unsuccessful primary canine pulpotomy: a case report

저자 : Haeni Kim

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 46권 3호 발행 연도 : 2022 페이지 : pp. 128-134 (7 pages)

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Pulpotomies are usually the treatment of choice for vital primary teeth where the removal of deep caries may lead to pulp exposure. These procedures entail removal of the inflamed coronal pulp, application of a pulp capping material, and preservation of the vitality of the radicular pulp. Inaccurate diagnosis or defective restorations may lead to failure of pulpotomies and consequent apical inflammation and cyst formation. The spread of apical inflammatory lesions affecting deciduous canines to the periodontal tissues can cause displacement and impaction of the permanent successor tooth. As this is a serious side effect of pulpotomies, precise diagnosis and long-term follow-up are required before and after the treatment. This case study presents three patients with canine impaction caused by unsuccessful pulpotomy of the deciduous canine.

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6An 8-year follow-up of autotransplantation into artificial socket with sinus elevation and autogenous bone grafting

저자 : Kyung-min Lee , Min-keun Kim , Young-wook Park

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 46권 3호 발행 연도 : 2022 페이지 : pp. 135-139 (5 pages)

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This case report presents the 8-year follow-up result of autotransplantation on a healed alveolar ridge, with sinus elevation and autogenous bone grafting. We present a case of a 30-year-old male patient whose maxillary first molar was extracted 1 year ago. Under general anesthesia, after the membrane of the left maxillary sinus was elevated, an artificial socket was prepared on the healed alveolar ridge of the left maxillary first molar area. The left maxillary third molar was extracted and tried on the prepared artificial socket. The autogenous bone was harvested and grafted to the left maxillary sinus floor. The transplanted tooth received root canal therapy 2 months after surgery. During the 8-year follow-up period, no discomforts, periodontal ligament (PDL) widening, or root resorption signs were observed. Desirable bone remodeling signs and regeneration of PDL were observed on the radiograph. Therefore, autotransplantation can be a treatment option for healed and deficient alveolar ridge.

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