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한국응용생명화학회> Journal of Applied Biological Chemistry> Eriodictyol induces apoptosis via regulating phosphorylation of JNK, ERK, and FAK/AKT in pancreatic cancer cells

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Eriodictyol induces apoptosis via regulating phosphorylation of JNK, ERK, and FAK/AKT in pancreatic cancer cells

Ui Hyeon Oh , Da-hye Kim , Jungwhoi Lee , Song-i Han , Jae-hoon Kim
  • : 한국응용생명화학회
  • : Journal of Applied Biological Chemistry 65권2호
  • : 연속간행물
  • : 2022년 06월
  • : 83-88(6pages)
Journal of Applied Biological Chemistry

DOI


목차

Introduction
Materials and Methods
Results
Discussion
References

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초록 보기

Although it has been intensively studied over the past few decades, pancreatic cancer remains one of the most lethal cancers. Eriodictyol, a plant-derived flavonoid mainly found in citrus fruits, exerts diverse biological effects, including antioxidant, anti-cancer, and anti-inflammatory properties. In this study, we investigated the anticancer properties of eriodictyol and its mechanisms of action in pancreatic cancer cells. In both SNU213 and Panc-1 cells, eriodictyol decreased viability, induced apoptosis, and decreased clonogenicity. In addition, eriodictyol treatment increased the phosphorylation level of JNK and decreased the phosphorylation levels of ERK, FAK, and AKT. These observations provide insight into the molecular mechanisms of eriodictyol-induced apoptosis in pancreatic cancer cell lines, and could contribute to the development of candidate compounds for treating pancreatic cancer.

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간행물정보

  • : 농학분야  > 농화학
  • : KCI등재
  • : SCOPUS
  • : 계간
  • : 1976-0442
  • : 2234-7941
  • : 학술지
  • : 연속간행물
  • : 1999-2022
  • : 1482


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110종 꿀풀과(Lamiaceae) 식물 종자 추출물의 항산화 활성

저자 : 김준혁 ( Junhyeok Kim ) , 이희호 ( Hee Ho Lee ) , 박충열 ( Chung Youl Park ) , 김현민 ( Hyun Min Kim ) , 정영호 ( Young Ho Jung ) , 김세현 ( Sae Hyun Kim ) , 나채선 ( Chae Sun Na )

발행기관 : 한국응용생명화학회 간행물 : Journal of Applied Biological Chemistry 65권 3호 발행 연도 : 2022 페이지 : pp. 121-128 (8 pages)

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본 연구는 꿀풀과 식물 종자 추출물의 항산화 활성 평가를 통해 식물 유래 천연 항산화제를 탐색하기 위해 진행되었다. 충실률 90% 이상이고 발아율이 50% 이상인 종자를 대상으로 하였다. 총 페놀성 화합물 함량은 속단(6.2 mg GAEs/g seed), 향유(4.5 mg GAEs/g seed) 순으로 높게 나타났다. 총 플라보노이드 함량은 향유(1.0 mg QEs/g seed), 속단(0.6 mg QEs/g seed) 순으로 높게 나타났다. DPPH 라디칼 소거활성은 종자 추출물의 EC50 값을 기준으로 향유(27.5 μg/mL), 쥐깨풀(29.2 μg/mL), 꿀풀(33.3 μg/mL) 순으로 높게 나타났다. ABTS 라디칼 소거활성은 꿀풀(25.6 μg/mL), 향유(25.9 μg/mL), 쥐깨풀(27.6 μg/mL) 순으로 높게 나타났다. FRAP 평가는 꿀풀(2910.4 μM Fe(II)/g extract), 향유(2836.2 μM Fe(II)/g extract), 쥐깨풀(2754.4 μM Fe(II)/g extract) 순으로 높게 나타났다. 항산화 활성을 기반으로 한 군집분석에 따라 10종은 항산화 활성이 낮은 group 1에서 활성이 높은 group 3까지 3개의 group으로 분류하였으며, 향유, 쥐깨풀, 꿀풀 종자는 group 3에 속하였다.


This study explored plant-derived natural antioxidants by evaluating the antioxidant activity of Lamiaceae plant seed extracts. Plants with the percentage of filled seeds at or above 90% and seed germination at or above 50% were selected. Of the ten species studied, the total phenolic content of the seeds was high in the species Phlomis umbrosa Turcz. (6.2 mg GAEs/g of seeds) and Elsholtzia ciliata (Thunb.) Hyl. (4.5 mg GAEs/g of seeds). The total flavonoid content of the seeds was high in E. ciliata (1.0 mg QEs/g of seeds) and P. umbrosa (0.6 mg QEs/g of seeds). Based on the EC50 value of the seed extracts, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity was high in the seeds of the plants E. ciliata (27.5 μg/mL), Mosla dianthera (Buch.-Ham. ex Roxb.) Maxim. (29.2 μg/mL), and Prunella vulgaris var. lilacina Nakai (33.3 μg/mL). In addition, 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity was high in P. vulgaris var. lilacina (25.6 μg/mL), E. ciliata (25.9 μg/mL), and M. dianthera (27.6 μg/mL) seeds. The ferric reducing antioxidant power of the seed extracts was high in P. vulgaris var. lilacina (2910.4 μM Fe(II)/g of extract), E. ciliata (2836.2 μM Fe(II)/g of extract), and M. dianthera (2754.4 μM Fe(II)/g of extract). According to the cluster analysis based on antioxidant activity, the seeds of the ten species were classified into three groups, from group 1 with low antioxidant activity to group 3 with high antioxidant activity; E. ciliata, M. dianthera, and P. vulgaris var. lilacina were classified as group 3.

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2식물공장에서 생산된 새싹인삼의 생육 시기에 따른 영양성분 및 항산화 활성 변화

저자 : 성진아 ( Jin A Seong ) , 이희율 ( Hee Yul Lee ) , 김수철 ( Su Cheol Kim ) , 조두용 ( Du Yong Cho ) , 정재각 ( Jea Gack Jung ) , 김민주 ( Min Ju Kim ) , 이애련 ( Ae Ryeon Lee ) , 정종빈 ( Jong Bin Jeong ) , 손기호 ( Ki-ho Son ) , 조계만 ( Kye Man Cho )

발행기관 : 한국응용생명화학회 간행물 : Journal of Applied Biological Chemistry 65권 3호 발행 연도 : 2022 페이지 : pp. 129-142 (14 pages)

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새싹인삼은 잎부터 뿌리까지 섭취가 가능하며 스마트 팜 또는 식물공장에서 재배 시 계절에 영향을 받지 않으며 농약을 사용하지 않아도 되는 이점이 있다. 본 연구에서는 새싹인삼의 최적 재배 시기를 확인하고 영양성분과 항산화 활성을 비교 분석하였다. 생육 시기에 따른 지방산과 무기질 함량은 큰 차이를 보이지 않았다. 총 아미노산 함량은 45일까지 약간 감소하였고 이후에는 증가하였으며 생육 65일 arginine 함량은 3309.11 mg/100 g으로 가장 높게 확인되었다. 총 ginsenoside 함량은 생육기간 동안 큰 변화가 없었다(25일 29.83 mg/g→45일 32.77 mg/g→65일 26.02 mg/g). Ginsenoside Rg2 (0.62 mg/g), Re (8.69 mg/g), Rb1 (4.75 mg/g) 및 Rd (3.47 mg/g)의 함량은 생육기간 중 45일에 가장 높았다. Phenolic acids와 flavonols 함량은 생육 45일 (338.6 및 1277.14 μg/g)까지 증가 후 65일까지 감소하였다. Phenolic acids와 flavonols의 주요 화합물은 각각 benzoic acid (99.03-142.33 μg/g)와 epigallocatechin (416.03-554.64 μg/g)로 확인되었다. DPPH (44.27%), ABTS (75.16%)와 hydroxyl (63.29%) 라디칼 소거활성 및 FRAP 환원력(1.573 OD573 nm) 또한 총 phenolics 및 총 flavonolids 함량과 마찬가지로 생육 45일에 가장 높은 활성을 보였다.


Ginseng sprouts, which can be eaten from leaves to roots, has the advantage of not having to use pesticides without being affected by the season by using smart farms. The optimal cultivation timing of sprout ginseng was checked and the nutritional content and antioxidant activity were compared and analyzed. The values of total fatty acids and total minerals were no significant changes during the growth periods. The contents of total amino acids were slightly decreased to 45 days and after increased to 65 days. When the growth period was 65 days, arginine had the highest content of 3309.11 mg/100 g. The total phenolic contents were high at 3.73 GAE mg/g on the 45 days, and the total flavonoid contents were also the highest at 9.04 RE mg/g on the 45 days. The contents of total ginsenoside was not noticeable for the growth periods (29.83 on 25 days→32.77 on 45 days→26.02 mg/g on 65 days). The ginsenoside Rg2 (0.62 mg/g), Re (8.69 mg/g), Rb1 (4.75 mg/g) and Rd (3.47 mg/g) had highest contents on 45 days during growth. The values of phenolic acids and flavonols were gradually increased to 45 days (338.6 and 1277.14 μg/g) and then decreased to 65 days. The major compounds of phenolic acids and flavonols were confirmed to benzoic acid (99.03-142.33 μg/g) and epigallocatechin (416.03-554.64 μg/g), respectively. The values of 2,2-diphenyl-1-picrylhydrazyl (44.27%), 2,4,6-azino-bis (3-ethylbenzothiazoline-6-sulphnoic acid) diammonium salt (75.16%), and hydroxyl (63.29%) radical scavenging activities and ferric reducing/antioxidant power (1.573) showed the highest activity on the 45 days as well as results of total phenolic and total flavonoid contents.

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3고지방식이를 제공한 마우스에서 여주와 아쉬아간다 발효추출물 조합의 항비만 효과

저자 : 최승연 ( Seung Yeon Choi ) , 박현아 ( Hyun A Park ) , 윤영걸 ( Young Geol Yoon )

발행기관 : 한국응용생명화학회 간행물 : Journal of Applied Biological Chemistry 65권 3호 발행 연도 : 2022 페이지 : pp. 143-152 (10 pages)

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본 연구는 고지방식이(HFD)를 제공한 비만동물모델에서 Momordica charanatia (MC)와 Withania somnifera (WS) 추출물의 혈청 콜레스테롤 및 내장지방 감소효과를 분석하였다. 유산균으로 발효한 MC와 WS 추출물의 조합(FMCWS) 및 비발효 추출물(MCWS)의 조합을 HFD로 유도된 비만마우스에 8주 동안 경구 투여하였다. 실험 기간 동안 체중, 식이섭취량, 총 콜레스테롤, 중성지방, HDL-콜레스테롤 수치를 분석하였다. 체중과 총 콜레스테롤 및 중성지방 수치는 정상대조(NC)군과 비교하여 HFD를 섭취한 HFD군에서 유의하게 증가했다. 그러나, 추출물의 투여로 인해 비만마우스의 체중증가가 감소되는 경향을 확인하였고 더불어 HDL-콜레스테롤의 증가와 함께 총 콜레스테롤 및 중성지방 수치를 유의적으로 감소시켰다. 복부지방 무게와 부고환 지방조직 내 지방세포의 크기는 NC군에 비해 HFD군에서 유의하게 증가하였다. 그러나, FMCWS와 MCWS를 각각 투여한 군은 HFD군에 비해 복부지방 무게와 부고환 지방세포의 크기가 유의하게 감소하였다. 또한, HFD군의 간 조직에서 관찰되는 거대 소포성 지방구의 침착이 이들 군에서는 현저하게 감소하였다. 이러한 결과는 MC와 WS 추출물의 조합이 체중 증가에 대한 감소 경향이 있을 뿐만 아니라 내장지방 및 혈청 지질 수준을 현저히 낮추는 데 강력한 시너지 효과가 있어 HFD 유발 비만마우스에서 항비만 활성을 향상시키는 효능이 있음을 시사한다.


We investigated the serum cholesterol and visceral fat lowering effects of Momordica charanatia (MC) and Withania somnifera (WS) extracts in high-fat diet (HFD)-fed mice. Combination of fermented MC and WS extracts (FMCWS) as well as that of non-fermented extracts (MCWS) were orally administered to HFD-induced obese mice along with the HFD supplementation for 8 weeks. During the experiment, body weight, food intake, and levels of total cholesterol, triglyceride and HDL-cholesterol were analyzed. Body weight and the levels of total cholesterol and triglycerides were significantly increased in the HFD-fed mice compared with the normal control (NC) group. However, supplementation of the extracts showed a tendency to reduce body weight gain and suppressed the levels of total cholesterol and triglyceride with the increment of HDL-cholesterol levels. Abdominal fat weight was significantly increased in the HFD group, and the size of adipocytes within the epididymal adipose tissue was markedly expanded compared with the NC group. However, in the FMCWS and MCWS groups, the abdominal fat weight was significantly reduced and the sizes of the adipocytes were noticeably diminished compared with those of the HFD-fed mice. Moreover, the deposition of giant vesicular fat cells observed in the liver tissue of the HFD group was prominently reduced in these groups. These results indicate that the combination of extracts from MC and WS tends to have potent synergic effects in reducing body weight gain as well as significantly lowering the visceral fat and the serum lipid levels, and thus improving anti-obesity efficacy in HFD-induced obese mice.

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4Phytochemical constituents of Lactuca serriola leaves and their content analysis by HPLC-UV

저자 : Juree Kim , Hak-dong Lee , Jungwon Choi , Sanghyun Lee

발행기관 : 한국응용생명화학회 간행물 : Journal of Applied Biological Chemistry 65권 3호 발행 연도 : 2022 페이지 : pp. 153-158 (6 pages)

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This study aimed to identify the phytochemical constituents of Lactuca serriola leaves and perform quantitative analysis of the methanol (MeOH) extract of L. serriola, L. indica, L. raddeana, L. sativa, and L. triangulata. Six compounds were isolated from the MeOH extracts of L. serriola using open column chromatography and identified as protocatechuic acid (1), caffeic acid (2), cynaroside (3), apigenin 7-glucuronide (4), luteolin (5), and apigenin (6) using 1H-, 13C-nuclear magnetic resonance, and mass spectrometry. Quantitative analysis of the six compounds was performed on the MeOH extract of Lactuca species using high-performance liquid chromatography (HPLC) and an ultraviolet (UV). A reverse-phased column was used, and the UV absorbance was set to 280 nm. The contents of compounds 2 and 3 were the highest (1.58 and 2.64 mg/g ext., respectively) in L. serriola extracts. However, compounds 4 and 6 were higher (1.46 and 0.40 mg/g ext., respectively) in L. triangulata. These results provide quantitative data for the application of Lactuca species in the pharmaceutical and functional food industries.

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5Genetic localization of epicoccamide biosynthetic gene cluster in Epicoccum nigrum KACC 40642

저자 : Eun Ha Choi , Si-hyung Park , Hyung-jin Kwon

발행기관 : 한국응용생명화학회 간행물 : Journal of Applied Biological Chemistry 65권 3호 발행 연도 : 2022 페이지 : pp. 159-166 (8 pages)

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Epicoccum nigrum produces epipyrone A (orevactaene), a yellow polyketide pigment. Its biosynthetic gene cluster was previously characterized in E. nigrum KACC 40642. The YES liquid culture of this strain revealed high-level production of epicoccamide (EPC), with an identity that was determined using liquid chromatography-mass spectrometry analysis and molecular mass search using the SuperNatural database V2 webserver. The production of EPC was further confirmed by compound isolation and nuclear magnetic resonance spectroscopy. EPC is a highly reduced polyketide with tetramic acid and mannosyl moieties. The EPC structure guided us to localize the hypothetical EPC biosynthetic gene cluster (BGC) in E. nigrum ICMP 19927 genome sequence. The BGC contains genes encoding highly reducing (HR)-fungal polyketide synthase (fPKS)-nonribosomal peptide synthetase (NRPS), glycosyltransferase (GT), enoylreductase, cytochrome P450, and N-methyltrasnferase. Targeted inactivation of the HR-fPKS-NRPS and GT genes abolished EPC production, supporting the successful localization of EPC BGC. This study provides a platform to explore the hidden biological activities of EPC, a bolaamphiphilic compound.

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6Inhibitory effects of artemether on collagen-induced platelet aggregation via regulation of phosphoprotein inducing PI3K/Akt and MAPK

저자 : Dong-ha Lee

발행기관 : 한국응용생명화학회 간행물 : Journal of Applied Biological Chemistry 65권 3호 발행 연도 : 2022 페이지 : pp. 167-172 (6 pages)

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Pathophysiological reaction of platelets in the blood vessel is an indispensable part of thrombosis and cardiovascular disease, which is the most common cause of death in the world. In this study, we performed in vitro assays to evaluate antiplatelet activity of artemether in human platelets and attempted to identify the mechanism responsible for protein phosphorylation. Artemether is a derivative of artemisinin, known as an active ingredient of Artemisia annua, which has been reported to be effective in treating malaria, and is known to function through antioxidant and metabolic enzyme inhibition. However, the role of artemether in platelet activation and aggregation and the mechanism of action of artemether in collagen-induced human platelets are not known until now. In this study, the effect of artesunate on collageninduced human platelet aggregation was confirmed and the mechanism of action of artemether was clarified. Artemether inhibited the phosphorylation of PI3K/Akt and Mitogen-activated protein kinases, which are phosphoproteins that are known to act in the signal transduction process when platelets are activated. In addition, artemether decreased TXA2 production and decreased granule secretion in platelets such as ATP and serotonin release. As a result, artemether strongly inhibited platelet aggregation induced by collagen, a strong aggregation inducer secreted from vascular endothelial cells, with an IC50 of 157.92 μM. These results suggest that artemether has value as an effective antithrombotic agent for inhibiting the activation and aggregation of human platelets through vascular injury.

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7케일 중 살충제 Flubendiamide의 잔류 특성

저자 : 김현진 ( Hyun-jin Kim ) , 황규원 ( Kyu-won Hwang ) , 선정훈 ( Jung-hun Sun ) , 이태현 ( Tae-hyun Lee ) , 정경수 ( Kyoung-su Jeong ) , 문준관 ( Joon-kwan Moon )

발행기관 : 한국응용생명화학회 간행물 : Journal of Applied Biological Chemistry 65권 3호 발행 연도 : 2022 페이지 : pp. 173-181 (9 pages)

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본 연구는 케일 중 flubendiamide의 잔류 특성을 조사하여 생산단계 잔류허용기준(PHRL, Pre-Harvest Residue Limit)을 설정하고자 하였으며, 세척용매 및 세척방법에 따른 제거효율을 알아보기 위해 수행되었다. 포장 시험은 포장 1 (경기도 안성시)과 포장 2(경기도 이천시)의 시설재배 하우스 두 곳에서 실시하였다. 농약의 안전사용기준에 따라 10일간격 2회 살포하고 0(2시간 후), 1, 2, 3, 5, 7, 10일차에 수확하였다. Flubendiamide의 생물학적 반감기는 액체 크로마토그래피와 탠덤 질량분석기(LC-MS/MS)를 결합하여 분석한 시료 중 농약의 소실곡선으로부터 산출되었다. 분석법상의 정량한계(MLOQ)는 0.01 mg/kg 이었으며, 10 MLOQ 및 MRL (0.7 mg/kg) 두 수준에서 회수율 시험을 한 결과 각각 104.2±3.6, 101.9±10.2%의 회수율을 나타냈다. 케일 중 flubendiamide의 감소상수는 포장 1에서 0.2437, 포장 2에서 0.1981이었다. 감소상수를 사용하여 얻은 PHRL 계산식은 다음과 같이 추정되었다: 수확 10일전 잔류농도가 8.0 mg/kg 미만이라면 수확일의 잔류농도는 MRL 수준 이하일 것으로 판단된다. 세척에 의한 flubendiamide의 평균 제거율은 식초(39.8%)로 세척하였을 때 가장 높았고, 베이킹소다(31.7%), 칼슘파우더(30.2%), 중성세제(27.2%), 수돗물(15.9%) 순이었다. 본 연구의 결과는 농민과 소비자 모두에게 안전한 농산물을 생산하거나 소비하는데 유용할 것 이다.


This study was carried out to investigate the residual characteristics of flubendiamide in kale to establish pre-harvest residue limits (PHRL) and the removal efficiency according to the washing solvent and method. Field tests were conducted at two different greenhouses, field 1 (Anseong-si, Gyeonggi-do) and field 2 (Incheon-si, Gyeonggi-do). According to the safe use guidelines kale was sprayed with flubendiamide twice every 10 days and harvested 0 (after 2 h), 1, 2, 3, 5, 7 and 10 days after the final application. The biological half-live of flubendiamide in kale was calculated based on dissipation curves of the pesticide in samples analyzed by liquid chromatography coupled with tandem mass spectrometry. In the analysis, method limits of quantitation (MLOQ) were 0.01 mg/kg, and recoveries performed with two different fortification levels of 10 MLOQ and maximum residue limit (0.7 mg/kg) were 104.2±3.6 and 101.9±10.2%, respectively. The dissipation rate constant of flubendiamide in kales were 0.2437 at field 1 and 0.1981 at field 2. PHRL calculation equations obtained using the dissipation constants estimated as follows: if the residual concentration of flubendiamide in kale on 10 days before harvest is less than 8.0 mg/kg, the residual concentration on the harvest would be under MRL. The removal of flubendiamide from kale was the greatest when it was washed with vinegar (39.8%), followed by baking soda (31.7%), calcium powder (30.2%), neutral detergent (27.2%), and tap water (15.9%). The results of this study would be useful for both farmers and consumers to produce or consume safe agricultural products.

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8계피(Cinnamomum cassia Presl) 추출물과 cinnamaldehyde의 alcohol dehydrogenase 저해 효과

저자 : 도재호 ( Jaeho Do ) , 인만진 ( Man-jin In ) , 김동청 ( Dong Chung Kim )

발행기관 : 한국응용생명화학회 간행물 : Journal of Applied Biological Chemistry 65권 3호 발행 연도 : 2022 페이지 : pp. 183-187 (5 pages)

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계피(Cinnamomum cassia Presl)의 열수 추출물은 alcohol dehydrogenase (ADH)의 활성을 저해하였고 IC50값은 45.6 μg/mL이었다. 계피 추출물의 ADH 저해 성분은 산과 열에 비교적 안정하였으나 휘발성을 가진 물질로 나타났다. 계피에서 ADH 저해 물질의 추출을 위한 최적 온도와 시간은 각각 80 ℃와 2시간이었다. 계피의 정유 성분 중 cinnamaldehyde가 ADH 저해의 주된 물질이었다. Cinnamaldehyde는 기질인 에탄올에 대해 ADH 활성의 경쟁적 저해제로 여겨진다. 따라서 계피 열수 추출물과 cinnamaldehyde는 ADH의 활성을 효과적으로 저해하는 숙취해소 천연소재로서의 활용가능성을 보여주었다.


The hot water extract from cinnamon (Cinnamomum cassia Presl) inhibited the activity of alcohol dehydrogenase (ADH) with IC50 value of 45.6 μg/mL. The ADH inhibitory components in cinnamon extract were relatively stable to acid and heat, but were found to be volatile. The optimum temperature and time for extracting the ADH inhibitory components from cinnamon were 80 ℃ and 2 h, respectively. Among the essential oils of cinnamon, cinnamaldehyde was the main substance for ADH inhibition. Cinnamaldehyde is considered a competitive inhibitor of ethanol to ADH. Therefore, the cinnamon extract and cinnamaldehyde showed the potential to be used as natural materials for relieving symptoms of a hangover.

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9참깨박 에탄올 추출물의 항산화 및 α-amylase및 α-glucosidase 저해 활성

저자 : 오영진주 ( Ying Jin Zhu Wu ) , 김명현 ( Myung Hyun Kim ) , 한영실 ( Young Sil Han )

발행기관 : 한국응용생명화학회 간행물 : Journal of Applied Biological Chemistry 65권 3호 발행 연도 : 2022 페이지 : pp. 189-194 (6 pages)

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기름을 착유하고 얻어지는 부산물인 참깨박의 활용과 이용가치를 높이기 위해 일반성분(수분, 탄수화물, 조단백질, 조지방, 조회분), 무기질을 측정하였고 탈지 처리한 후 70% 에탄올 추출하여 항산화 활성 및 항당뇨 활성을 분석하였다. 참깨박의 일반성분 함량은 수분 6.51%, 탄수화물 16.22%, 조단백질 46.30%, 조회분 9.88%, 조지방 21.09%로 이었고, 무기질은 K 1128.08 mg/100 g, Ca 1356.27 mg/100 g, Fe 12.29 mg/100 g, P 2022.14 mg/100 g, Cu 2.08 mg/100 g, Mg 643.40 mg/100 g, Na 7.29 mg/100 g 함량을 나타내었다. 탈지 참깨박의 총 폴리페놀과 플라보노이드 함량은 각각 184.98 mg GAE/g, 27.63 mg QE/g였으며, DPPH 및 ABTS 라디칼 소거활성 IC50을 측정한 결과 각각 91.84, 340.09 μg/mL, 환원력은 0.87 OD으로 측정되었다. 탈지 참깨박의 α-amylase 및 α-glucosidase 저해 활성 IC50의 측정 결과는 각각 74.31, 16.73 mg/mL을 나타내었다. 따라서 70% 에탄올로 추출한 탈지 참깨박은 우수한 항산화 활성과 항당뇨 활성을 나타내 기능적 가치를 높일 수 있을 것으로 기대된다. 또한 참깨를 착유하고 남은 참깨박을 사용하게 되므로 환경보호와 자원순환 측면에 활용가치가 있을 뿐만 아니라 건강기능식품소재로 이용가치가 있을 것이라 판단된다.


In this study, a sesame meal was used in order to analyze the proximate composition and mineral contents. The sesame seed meal, pressed from roasted Sesame seed, contains various polyphenols. The defatted sesame meal was extracted using 70% ethanol, and its antioxidant activity and antidiabetic effects were evaluated. Proximate composition of sesame meal was showed that moisture 6.51%, carbohydrate 16.22%, crud protein 46.30%, ash 9.88%, crude fat 21.09%. Mineral contents were K 1128.08 mg/100 g, Ca 1356.27 mg/100 g, Fe 12.29 mg/100 g, P 2022.14 mg/100 g, Cu 2.08 mg/100 g, Mg 643.40 mg/100 g, Na 7.29 mg/100 g. The results showed the sesame meal of 70% ethanol extract had higher polyphenol content (184.98 mg GAE/g) and flavonoid content (27.63 mg QE/g). The 2,2-diphenyl-1-picrylhydra-zyl and 2,2′-aziono-bis(3-ethylbenzthiazoline-6-sulfonic acid radical scavenging activities of defatted sesame meal (IC50) were 891.84 and 340.09 μg/mL. According to the test results, the defatted sesame meal extracted using 70% ethanol had significant antioxidant activity and inhibitory ability to diabetes-related enzymes, indicating that it has good potential as a functional food or nutritional food for prevention and treatment of oxidation.

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10콩잎 열수추출물의 지표성분인 camelliaside A의 정량분석을 위한 HPLC-UVD 분석법 밸리데이션

저자 : 김정호 ( Jeong Ho Kim ) , 리승환 ( Seung Hwan Lee ) , 문시원 ( Si Won Moon ) , 박기훈 ( Ki Hun Park )

발행기관 : 한국응용생명화학회 간행물 : Journal of Applied Biological Chemistry 65권 3호 발행 연도 : 2022 페이지 : pp. 195-202 (8 pages)

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콩잎의 기능성식품소재연구가 활발히 진행되고 있지만 기능성 식품소재로서 콩잎을 관리할 수 있는 밸리데이션 방법은 제시되지 않고있다. 본 연구에서는 콩잎에서 7개의 kaempferol 유도체를 UHPLC-ESI-Q-TOF-MS로 동정하고 이들 중 camelliaside A를 지표물질로 선정하였다. 콩잎열수추출물에서 camelliaside A를 정량할 수 있는 HPLC-UVD 밸리데이션 방법을 식품의약품안전처의 건강기능성식품에 대한 가이드라인에 따라 구축하였다. 개발된 HPLC-UVD 밸리데이션 방법은 특이성, 정확도, 일내정밀도, 일간정밀도, 검출한계, 정량한계 및 직선성에서 가이드라인을 만족하는 결과값으로 검증되었다. 또한 camelliaside A 분석법을 콩잎열수추출물에 적용하여 적합한 수준의 평가값을 얻었다.


Soybean (Glycine max L.) leaves have been researched as functional food stuff actively, but there is no validation method to control quality of soybean leaves (SL). In this study, we annotated seven kaempferol derivatives to confirm camelliaside A as index metabolite in SL using UHPLC-ESI-TOF-MS. HPLC-UVD validation method of camelliaside A in hot-water extract of SL was established according to validation guideline of functional foods from the Ministry of Food and Safety of Korea. The HPLC-UVD method was validated with reliable parameters for examining specificity, accuracy, precision, limit of detection and quantification and linearity. The established method gave the suitable ranges to qunatitate camelliaside A from the hot-water extract of soybean leaves.

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1Eriodictyol induces apoptosis via regulating phosphorylation of JNK, ERK, and FAK/AKT in pancreatic cancer cells

저자 : Ui Hyeon Oh , Da-hye Kim , Jungwhoi Lee , Song-i Han , Jae-hoon Kim

발행기관 : 한국응용생명화학회 간행물 : Journal of Applied Biological Chemistry 65권 2호 발행 연도 : 2022 페이지 : pp. 83-88 (6 pages)

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Although it has been intensively studied over the past few decades, pancreatic cancer remains one of the most lethal cancers. Eriodictyol, a plant-derived flavonoid mainly found in citrus fruits, exerts diverse biological effects, including antioxidant, anti-cancer, and anti-inflammatory properties. In this study, we investigated the anticancer properties of eriodictyol and its mechanisms of action in pancreatic cancer cells. In both SNU213 and Panc-1 cells, eriodictyol decreased viability, induced apoptosis, and decreased clonogenicity. In addition, eriodictyol treatment increased the phosphorylation level of JNK and decreased the phosphorylation levels of ERK, FAK, and AKT. These observations provide insight into the molecular mechanisms of eriodictyol-induced apoptosis in pancreatic cancer cell lines, and could contribute to the development of candidate compounds for treating pancreatic cancer.

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2Soil conditions during cultivation affect the total phenolic and flavonoid content of rosemary

저자 : Ji Won Seo , Soo Kyung Kim , Ji Hye Yoo , Myong Jo Kim , Eun Soo Seong

발행기관 : 한국응용생명화학회 간행물 : Journal of Applied Biological Chemistry 65권 2호 발행 연도 : 2022 페이지 : pp. 89-92 (4 pages)

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In this study, the effects of soil conditions on antioxidant activities of the aerial and underground parts of rosemary were assessed to determine the most effective soil conditions for cultivation. The antioxidant activity was the highest (51.58±2.93 μg/mL) when cultivated in the mixture of gardening soil and vermiculite using DPPH assay. The antioxidant activity of underground parts the highest (127.48±12.38 μg/mL) when cultivated in the mixture of soil, vermiculite, and perlite. ABTS assay showed that the antioxidant activity of aerial parts was 230.34±57.93 μg·mL-1 when cultivated in the mixture of gardening soil and vermiculite and that of underground parts was 320.98±16.04 μg·mL-1 when cultivated in the mixture of gardening soil, vermiculite, and perlite. The total phenolic content of aerial parts was the highest (155.25±2.96 mg GAE/g) when cultivated in the mixture of gardening soil. The total flavonoid content of aerial parts was the highest (67.32±5.27 mg QE/g) when cultivated in the mixture of gardening soil. Therefore, the mixture of gardening soil, vermiculite, and perlite is superior to gardening soil alone for cultivation of rosemary to increase its antioxidant activity as well as total phenolic and flavonoid content.

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3The inhibitory effect of egg white lysosome extract (LOE) on melanogenesis through ERK and MITF regulation

저자 : Jung Eun Park , Hyung Seo Hwang

발행기관 : 한국응용생명화학회 간행물 : Journal of Applied Biological Chemistry 65권 2호 발행 연도 : 2022 페이지 : pp. 93-99 (7 pages)

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Lysosome organelle extract (LOE) was derived from egg whites. The lysosome is an intracellular organelle that contains several hydrolysis enzymes. Previous studies have reported that LOE performs important functions, such as melanin decolorization and anti-melanin production in B16F10 melanoma cells. However, its principal molecular and cellular mechanisms have not been elucidated till date. In non-cytotoxic conditions, LOE significantly inhibited α-MSH induced melanin synthesis of murine B16F10 cells. The anti-melanogenic activity of LOE was mediated by suppressing the mRNA expression of tyrosinase enzyme, tyrosinase related protein-1/2 (TRP-1/2), and microphthalmiaassociated transcription factor (MITF) genes. By performing western blot analysis, we found that LOE significantly attenuated melanogenesis. In this case, LOE helped in increasing extracellular receptor kinase (ERK) phosphorylation in α-MSH induced B16F10 cells. Furthermore, MITF is found to be a key regulatory transcription factor in melanin synthesis; it was down-regulated by LOE through ERK phosphorylation. In this experiment, PD98059 (MEK inhibitor) was used to check whether LOE directly regulated the activity of ERK. Although LOE exerted inhibitory effect on melanin synthesis, we could not observe this effect in PD98059-treated α-MSH induced B16F10. These results strongly indicate that LOE is related to ERK activation and MITF degradation in anti-skin pigmentation. Hence, LOE should be utilized as a whitening agent of skin in the near future.

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4Quercetin의 식물병원성 세균인 Xanthomonas campestris에 대한 선택적 살균활성

저자 : 김정윤 ( Jeong Yoon Kim ) , 김주연 ( Ju Yeon Kim ) , 서성준 ( Sung-jun Seo ) , 서민경 ( Min-kyung Seo ) , 김진성 ( Jin-seong Kim ) , 강상우 ( Sang-woo Kang ) , 오경열 ( Kyeong-yeol Oh ) , 김진효 ( Jin-hyo Kim )

발행기관 : 한국응용생명화학회 간행물 : Journal of Applied Biological Chemistry 65권 2호 발행 연도 : 2022 페이지 : pp. 101-105 (5 pages)

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양파의 주요 flavonoid 성분인 quercetin에 대한 항균 활성은 일부 알려져 있으나, 식물 병원성 세균인 X. campestris와 E. carotovora에 대한 활성은 알려져 있지 않았다. 본 연구에서는 quercetin과 양파 추출물을 활용하여 이들 식물병원균에 대한 항균 활성과 농용 항생제인 streptomycin과의 synergistic 효과를 평가하였으나, E. carotovora에 대한 항균 활성은 없었다. 하지만, X. campestris에서는 다른 세균류에서 알려진 MIC보다 10배 이상 낮은 15.6 μg mL-1이었으며, MBC는 20.0 μgmL-1으로 X. campestris에 대해 높은 선택적 항균 활성을 확인할 수 있었다. 다만, 농업용 항생제로 사용중인 streptomycin과의 약효상승 효과는 없었다. 또한, quercetin은 양파껍질의 EtOAc와 EtOH 추출물로부터 확보할 수 있었으며, EtOAc 추출물에서 quercetin 순도가 높음을 확인하였고, 이에 따라 EtOH 추출물보다 2배 가량 높은 항균 활성(MIC = 500 μg mL-1)이 확인되었다.

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5Biological activities of ethanolic extract from Robinia pseudoacacia L. flower

저자 : Myeong Gyu Han , Yu Jin Park , Man-jin In , Dong Chung Kim

발행기관 : 한국응용생명화학회 간행물 : Journal of Applied Biological Chemistry 65권 2호 발행 연도 : 2022 페이지 : pp. 107-111 (5 pages)

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Biological activities such as antioxidant, anticoagulant, and α-glucosidase inhibitory effects of 40% (v/v) ethanolic extract from black locust (Robinia pseudoacacia L.) flower were investigated. The polyphenol content of the black locust flower extract was 39.8±0.5 mg gallic acid equivalents/g. The flower extract represented antioxidant effects such as free radical, cationic radical, and nitrite scavenging abilities as well as reducing power. Also the flower extract inhibited α-glucosidase activity and common pathway in plasma coagulation system.

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6국립대전현충원에서 분리한 남조류 구슬말(Nostoc commune)의 항염증 효과

저자 : 홍혜현 ( Hyehyun Hong ) , 배은희 ( Eun Hee Bae ) , 박태진 ( Tae-jin Park ) , 강민성 ( Min-sung Kang ) , 강재신 ( Jae Shin Kang ) , 지원재 ( Won-jae Chi ) , 김승영 ( Seung-young Kim )

발행기관 : 한국응용생명화학회 간행물 : Journal of Applied Biological Chemistry 65권 2호 발행 연도 : 2022 페이지 : pp. 113-120 (8 pages)

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구슬말(Nostoc commune Vaucher ex Bornet & Flahault)은 이형세포를 갖는 특징으로 다른 목들과 구분되는 남조류의 일종으로 극지방에서 열대지역까지 광범위한 지역에 분포하며 단세포가 연결되어 형성된 수많은 trichome들이 점액질에 둘러 쌓인 형태로 커다란 군체를 형성한다. 주로 토양, 암반, 잔디 위 등에 서식한다고 알려져 있으나 흔히 관찰되지 않기 때문에 현재 연구가 거의 없는 실정이다. 이에 본 연구에서는 토양 남조류인 N. commune HCW0811을 분리 및 동정하였으며 항염증 활성을 조사 하고자 하였다. 그 결과 N. commune HCW0811는 LPS로 유도된 RAW 264.7세포에서 80%이상의 세포 생존율을 나타내었으며 NO, PGE2 및 TNF-α, IL-6, IL-1β의 생성을 효과적으로 억제하였다. 또한 western blot assay를 통해 iNOS, COX-2 및 MAP kinase (p38, ERK1/2, JNK)와 NF-κB 세포내 신호전달 경로에서의 단백질 발현을 조사한 결과 이들의 발현이 유의하게 억제됨을 확인하였다. 본 연구에서는 이러한 결과를 근거하여 HCW0811가 다양한 염증 인자를 표적으로 하는 피부 면역 질환을 포함한 염증성 질환의 예방과 치료를 위한 항염증 기능성 화장품 및 식품소재로의 개발가능성을 제시한다.

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발행기관 최신논문
자료제공: 네이버학술정보

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