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한국생약학회> 생약학회지> 만성 역류성 식도염에서 황련과 오수유 혼합물이 식도 점막에 미치는 영향

KCI등재SCOUPUS

만성 역류성 식도염에서 황련과 오수유 혼합물이 식도 점막에 미치는 영향

Effect of Rhei Rhizoma and Scutellariae Radix Mixture in Chronic Acid Reflux Esophagitis Rats

이진아 ( Jin A Lee ) , 오민혁 ( Min Hyuck Oh ) , 신미래 ( Mi-rae Shin ) , 노성수 ( Seong-soo Roh ) , 박해진 ( Hae-jin Park )
  • : 한국생약학회
  • : 생약학회지 52권3호
  • : 연속간행물
  • : 2021년 09월
  • : 177-185(9pages)
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Gastroesophageal reflux disease (GERD) is a gastrointestinal disorder in which stomach contents reflux into the esophagus, causing complications such as mucosal damage. The purpose of this study was to determine the effect of Rhei Rhizoma and Scutellariae Radix mixture (RS) in chronic acid reflux esophagitis (CARE), one of the GERD. After inducing reflux esophagitis through surgery, the group was separated and the drug was administered for 2 weeks; Normal rats (Normal, n=8), chronic acid reflux esophagitis rats (Control, n=8), tocopherol 30 mg/kg-treated chronic acid reflux esophagitis rats (Toco, n=8), Rhei Rhizoma and Scutellariae Radix mixture 100 mg/kg-treated chronic acid reflux esophagitis rats (RSL, n=8), Rhei Rhizoma and Scutellariae Radix mixture 200 mg/kg-treated chronic acid reflux esophagitis rats (RSH, n=8). Gross lesion of esophageal mucosa after RS treatment showed a superior enhancement compared with that of Control group. Additionally, RS significantly decreased the levels of MPO and MDA, effectively inhibited NADPH oxidase, and regulated the expression of the AMPK/ LKB1/NF-κB pathway. Moreover, it significantly increased the expression of tight junction proteins. Taken together, RS not only alleviates inflammation of the esophageal mucosa via the AMPK/LKB1/NF-κB pathway by reducing oxidative stress, but also improves esophageal function by modulating tight junction proteins.

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간행물정보

  • : 의약학분야  > 약화학
  • : KCI등재
  • : SCOPUS
  • : 계간
  • : 0253-3073
  • : 2288-9299
  • : 학술지
  • : 연속간행물
  • : 1970-2021
  • : 3344


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KCI등재 SCOPUS

1Esculetin이 PI3K/MAPK 경로 하향 조절을 통해 collagen 유도의 혈소판 응집 억제에 미치는 효과

저자 : 박창은 ( Chang-eun Park ) , 이동하 ( Dong-ha Lee )

발행기관 : 한국생약학회 간행물 : 생약학회지 52권 3호 발행 연도 : 2021 페이지 : pp. 1-7 (7 pages)

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Platelet activation plays a major role in cardiovascular disorders (CVDs). Thus, disrupting platelet activation represents an attractive therapeutic target on CVDs. Esculetin, a bioactive 6,7-dihydroxy derivative of coumarin, possesses pharmacological activities against obesity, diabetes, renal failure, and CVDs. In other report, the effect of esculetin has been examined in human platelet activation and experimental mouse models, and esculetin inhibited collagen- and arachidonic acid-induced platelet aggregation in washed human platelets. However, it had no effects on other agonists such as thrombin and U46619, and its mechanism is not also clearly known. This study investigated the effect of esculetin on collagen-induced human platelet aggregation, and we clarified the mechanism. Esuletin has effects on the down regulation of PI3K/Akt and MAPK, phosphoproteins that act in the signaling process in platelet aggregation. The effects of esculetin reduced of TXA2 production and phospholipase A2 activation, and intracellular granule secretion including ATP and serotonin, leading to inhibit platelet aggregation. These results clearly clarified the effect of esculetin in inhibiting platelet activity and thrombus formation in humans.

KCI등재 SCOPUS

2참가죽그물바탕말 1,9-Dihydroxycrenulide 및 Epiloliolide의 양모 효능

저자 : 강정일 ( Jung-il Kang ) , 오태헌 ( Tae Heon Oh ) , 김지오 ( Jioh Kim ) , 노해민 ( Haemin No ) , 이남호 ( Nam Ho Lee ) , 유은숙 ( Eun-sook Yoo ) , 강희경 ( Hee-kyoung Kang )

발행기관 : 한국생약학회 간행물 : 생약학회지 52권 3호 발행 연도 : 2021 페이지 : pp. 134-142 (9 pages)

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This study was conducted to evaluate the effects of Dictyota coriacea extract and its active components such as 1,9- dihydroxycrenulide and epiloliolide on the hair growth. Treatment with D. coriacea extract and the hexane and EtOAc fractions of D. coriacea extract significantly increased the proliferation of dermal papilla cells (DPCs), a central regulator of the hair cycle. Especially, 1,9-dihydroxycrenulide and epiloliolide from D. coriacea extract, caused an increase in the DPC proliferation. When isolated rat vibrissa follicles were treated with 1,9-dihydroxycrenulide or epiloliolide for 21 d, the hair-fiber lengths for the vibrissa follicles increased. When examined the activity of 5α-reductase, which converts testosterone to dihydrotestosterone (DHT), a main cause of androgenetic alopecia, the several solvent fractions of D. coriacea extract significantly decreased the 5α-reductase activity while 1,9-dihydroxycrenulide and epiloliolide scarcely inhibited 5α-reductase activity. In addition, we found that the D. coriacea extract and several solvent fractions of D. coriacea extract could not act as a KATP channel opener, which could be a contributory factor in the effect on hair growth. These results suggest that D. coriacea extract and 1,9-dihydroxycrenulide and epiloliolide, principals of D. coriacea, have the potential to treat alopecia via the proliferation of DPCs.

KCI등재 SCOPUS

3거품돌산호 추출물의 멜라닌 합성 억제 효능

저자 : 심중현 ( Joong Hyun Shim )

발행기관 : 한국생약학회 간행물 : 생약학회지 52권 3호 발행 연도 : 2021 페이지 : pp. 143-148 (6 pages)

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This study was performed to elucidate the inhibitory effects of Alveopora japonica extract on melanin synthesis by measuring the levels of cell viability, mRNA expression, tyrosinase activity, and melanin production in the B16F10 cell line. The effects of A. japonica extract on tyrosinase-related protein 1 (TYRP1), TYRP2, tyrosinase (TYR), and microphthalmia-associated transcription factor (MITF) mRNA expression levels and melanin content were determined. Quantitative real-time RTPCR show that A. japonica extract decrease the mRNA expression levels of TYRP1, TYRP2, TYR, and MITF in B16F10 cell line, resulting in lower levels of melanin production compared to α-MSH-treated B16F10 cells. Tyrosinase activity assays reveal that A. japonica extract decrease melanin production in B16F10 cells. These results demonstrate the whitening effects of A. japonica extract on B16F10 cells; thus, A. japonica extract is a potent ingredient for skin whitening. Further research is needed on the mechanism of action of A. japonica extract. Such research will benefit not only cosmetics, but also the health food and medical industries.

KCI등재 SCOPUS

4스피루리나 에탄올 추출물의 신경세포 보호활성

저자 : 류가희 ( Gahee Ryu ) , 마충제 ( Choong Je Ma )

발행기관 : 한국생약학회 간행물 : 생약학회지 52권 3호 발행 연도 : 2021 페이지 : pp. 149-156 (8 pages)

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Excessive glutamate can cause oxidative stress in neuronal cells and this can be the reason for neurodegenerative disease. In this study, we investigated the protective effect of Spirulina maxima hot ethanol extract on mouse hippocampal HT22 cell of which glutamate receptor has no function. HT22 cells were pre-treated with S. maxima sample at a dose dependent manner (1, 10 and 100 μg/ml). After an hour, glutamate was treated. Cell viability, reactive oxygen species (ROS) accumulation, Ca 2+ influx, decrease of mitochondrial membrane potential level and glutathione related assays were followed by then. S. maxima ethanol extract improved the cell viability by suppressing the ROS and Ca 2+ formation, retaining the mitochondrial membrane potential level and protecting the activity of the antioxidant enzymes compared with group of vehicle-treated controls. These suggest that S. maxima may decelerate the neurodegeneration by attenuating neuronal damage and oxidative stress.

KCI등재 SCOPUS

5마이크로웨이브 추출방법을 이용한 작약의 유효성분 추출 및 생리활성 측정

저자 : 이효진 ( Hyojin Lee ) , 장경원 ( Kyoung Won Jang )

발행기관 : 한국생약학회 간행물 : 생약학회지 52권 3호 발행 연도 : 2021 페이지 : pp. 157-162 (6 pages)

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The heat-mediated reflux apparatus extraction of Paeonia lactiflora Pall. has been widely used as a traditional extraction method. In this paper, the microwave apparatus extraction method of Paeonia Radix was performed and the active ingredients and functional constituents were compared with the reference extraction method. The most effective extraction condition of albiflorin using the microwave was 120℃ in 50% methanol, and paeoniflorin was maximally extracted at 60℃. The reduced level of paeoniflorin molecule at high-pressure and high-temperature extraction condition was caused by the molecular instability. Additionally, the microwave extraction of 50% methanol extracts at 150℃ showed the highest functional constituents determined by in vitro DPPH radical scavenging activity, polyphenol concentration, and tyrosinase inhibition assay. The microwave apparatus was adapted as a rapid, low-cost, and environmentally friendly method to extract active ingredients and the practical extraction conditions of Paeonia Radix can be used in industrial applications.

KCI등재 SCOPUS

6구증구포 맥문동 Ethyl acetate 분획물의 예쁜 꼬마선충 내의 항산화 효과

저자 : 권강무 ( Kang Mu Kwon ) , 김준형 ( Jun Hyeong Kim ) , 양재헌 ( Jae Heon Yang ) , 기별희 ( Byeolhui Ki ) , 황인현 ( In Hyun Hwang ) , 김대근 ( Dae Keun Kim )

발행기관 : 한국생약학회 간행물 : 생약학회지 52권 3호 발행 연도 : 2021 페이지 : pp. 163-169 (7 pages)

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Using the Caenorhabditis elegans model system, the antioxidant activity of methanol extract of the guzeunggupoprocessed Liriope platyphylla F. T. Wang (Liliaceae) tuber was calculated. Between the methanol extracts of guzeunggupo-processed and non-processed L. platyphylla tuber, the processed L. platyphylla tuber showed higher DPPH radical scavenging effect than the non-processed one. The ethyl acetate soluble fraction of the methanol extract of the guzeunggupo-processed L. platyphylla tuber showed the best DPPH radical scavenging activity. The ethyl acetate fraction of the processed sample was measured for the activities of superoxide dismutase (SOD), catalase, and oxidative stress tolerance by using C. elegans along with reactive oxygen species level. In addition, to verify the regulation of the stress response gene is responsible for the increased stress tolerance of C. elegans treated by the ethyl acetate fraction of the processed sample, SOD-3 expression was measured using a transgenic strain (CF1553). Consequently, the ethyl acetate fraction of the processed sample, increased SOD and catalase activities, and decreased ROS accumulation in a dose-dependent manner. Furthermore, the ethyl acetate fraction of the processed sample-treated CF1553 worm showed higher SOD-3::GFP intensity than the control worm.

KCI등재 SCOPUS

7Cyclophosphamide에 의해 유도된 면역 저하 마우스에서 흑미 (Oryza sativa L.) 호분층의 면역 활성 효과

저자 : 이은별 ( Eun Byeol Lee ) , 최지혜 ( Ji-hye Choi ) , 김시현 ( Si Hyun Kim ) , 장환희 ( Hwan Hee Jang ) , 이성현 ( Sung Hyen Lee )

발행기관 : 한국생약학회 간행물 : 생약학회지 52권 3호 발행 연도 : 2021 페이지 : pp. 170-176 (7 pages)

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Anthocyanins are considered as one of the major functional components including cyanidin-3-glucoside and peonidin- 3-glucoside anthocyanins of black rice (Oryza sativa L., BR) aleurone layer (OAL) extract. The immunomodulatory biological effects of OAL has rarely been evaluated. This study has extensively studied the immunomodulatory effects of BR in immunosuppressed mice by CPA. The immunostimulatory effects of BR extract in in vivo at 150 (OAL-1) and 300 mg/kg body weight (OAL-2) for OAL groups were measured and compared to the normal group (CON) ingested with distilled water only or other groups. After the 14 days of oral administration, immune related organ weights, serum immunoglobulin (Ig) A and IgG, and cytokines (IL-12, TNF-α) levels, splenocytes proliferation rate, and NK cell activity were evaluated as immune related biomarkers. The serum IgA and IgG values in the OAL-treated groups increased to the level of the CON. OAL significantly and effectively controlled the IL-12 and TNF-α levels and NK activity compared to the negative control (NC) group. The results suggest that BR aleurone layer with immunomodulatory activities has a great potential as a functional food by itself or as a supplement.

KCI등재 SCOPUS

8만성 역류성 식도염에서 황련과 오수유 혼합물이 식도 점막에 미치는 영향

저자 : 이진아 ( Jin A Lee ) , 오민혁 ( Min Hyuck Oh ) , 신미래 ( Mi-rae Shin ) , 노성수 ( Seong-soo Roh ) , 박해진 ( Hae-jin Park )

발행기관 : 한국생약학회 간행물 : 생약학회지 52권 3호 발행 연도 : 2021 페이지 : pp. 177-185 (9 pages)

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Gastroesophageal reflux disease (GERD) is a gastrointestinal disorder in which stomach contents reflux into the esophagus, causing complications such as mucosal damage. The purpose of this study was to determine the effect of Rhei Rhizoma and Scutellariae Radix mixture (RS) in chronic acid reflux esophagitis (CARE), one of the GERD. After inducing reflux esophagitis through surgery, the group was separated and the drug was administered for 2 weeks; Normal rats (Normal, n=8), chronic acid reflux esophagitis rats (Control, n=8), tocopherol 30 mg/kg-treated chronic acid reflux esophagitis rats (Toco, n=8), Rhei Rhizoma and Scutellariae Radix mixture 100 mg/kg-treated chronic acid reflux esophagitis rats (RSL, n=8), Rhei Rhizoma and Scutellariae Radix mixture 200 mg/kg-treated chronic acid reflux esophagitis rats (RSH, n=8). Gross lesion of esophageal mucosa after RS treatment showed a superior enhancement compared with that of Control group. Additionally, RS significantly decreased the levels of MPO and MDA, effectively inhibited NADPH oxidase, and regulated the expression of the AMPK/ LKB1/NF-κB pathway. Moreover, it significantly increased the expression of tight junction proteins. Taken together, RS not only alleviates inflammation of the esophageal mucosa via the AMPK/LKB1/NF-κB pathway by reducing oxidative stress, but also improves esophageal function by modulating tight junction proteins.

KCI등재 SCOPUS

9곰보배추에 함유된 6-Hydroxyluteolin 7-O-Glucoside 분석을 위한 HPLC/DAD 분석법 밸리데이션

저자 : 이학동 ( Hak-dong Lee ) , 바헤 ( Leo Adrianne Paje ) , 최중원 ( Jungwon Choi ) , 김주리 ( Juree Kim ) , 유아람 ( A Ram Yu ) , 배민정 ( Min-jung Bae ) , 이상현 ( Sanghyun Lee )

발행기관 : 한국생약학회 간행물 : 생약학회지 52권 3호 발행 연도 : 2021 페이지 : pp. 186-191 (6 pages)

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We developed a method to identify and quantify 6-hydroxyluteolin 7-O-glucoside in the powder of Salvia plebeia (PS) using high-performance liquid chromatography coupled with diode array detector (HPLC/DAD) and equipped with reverse-phase INNO C18 column. The analytical method was optimized and validated using novel parameters. The obtained values for the limits of detection and quantification were 3.60 and 10.90 μg/mL, respectively. Calibration curve showed good linearity in the concentration range tested (0.00625-0.1 mg/mL, r 2 = 1.0000), high accuracy (96.2-101.4%), and precision values (RSD ≤ 0.27%). Our analysis support the use of our method for accurately identifying and quantifying 6-hydroxyluteolin 7-Oglucoside from PS in routine analyses and large-scale extraction processes for content determination.

KCI등재 SCOPUS

10제조방법에 따른 당귀수산(當歸鬚散)의 성분분석 및 항염증 효과

저자 : 전영희 ( Young-hee Jeon ) , 남원희 ( Won-hee Nam ) , 임현희 ( Hyun-hee Leem ) , 김세진 ( Se-jin Kim ) , 유병우 ( Byung-woo Yu ) , 손수미 ( Su-mi Son ) , 김명진 ( Myoung-jin Kim ) , 최혜민 ( Hye-min Choi ) , 권현숙 ( Hyun-sook Kwon ) , 김정옥 ( Jung-ok Kim )

발행기관 : 한국생약학회 간행물 : 생약학회지 52권 3호 발행 연도 : 2021 페이지 : pp. 192-201 (10 pages)

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Dangguisu-san (DGSS) is widely known traditional herbal medicinal formula in Korea for treatment of traumatic injury by traffic accident, ecchymosis, abdominal distension and anti-thrombosis of blood. This study was conducted to develop the simultaneous analyze method using high performance liquid chromatography (HPLC) and examine the effect of anti-inflammatory activity of DGSS-dry extract (DGSS-DE) and DGSS-mix extract powder (DGSS-MEP). Physicochemical characteristics of DGSS-DE and DGSS-MEP showed that there is no significant difference in pH, titratable acidity, total soluble solid content and browning degree except for color value (L, a, b). 15 functional constituents of DGSS were identified and the correlation coefficient values of DGSS-DE and DGSS-MEP were conformed 0.950. Also, DGSS-DE and DGSS-MEP significantly decreased the secretion of nitric oxide (NO), prostaglandin E2 (PGE2), interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) through inhibited expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), IL-1β, IL-6, and TNF-α. From these result, DGSS-MEP showed similar chemical composition and anti-inflammatory effect to DGSS-DE. Therefore, DGSS-DE and DGSS-MEP may be useful as potential source of drug to prevent inflammation.

1
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KCI등재SCOUPUS

1Esculetin이 PI3K/MAPK 경로 하향 조절을 통해 collagen 유도의 혈소판 응집 억제에 미치는 효과

저자 : 박창은 ( Chang-eun Park ) , 이동하 ( Dong-ha Lee )

발행기관 : 한국생약학회 간행물 : 생약학회지 52권 3호 발행 연도 : 2021 페이지 : pp. 1-7 (7 pages)

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Platelet activation plays a major role in cardiovascular disorders (CVDs). Thus, disrupting platelet activation represents an attractive therapeutic target on CVDs. Esculetin, a bioactive 6,7-dihydroxy derivative of coumarin, possesses pharmacological activities against obesity, diabetes, renal failure, and CVDs. In other report, the effect of esculetin has been examined in human platelet activation and experimental mouse models, and esculetin inhibited collagen- and arachidonic acid-induced platelet aggregation in washed human platelets. However, it had no effects on other agonists such as thrombin and U46619, and its mechanism is not also clearly known. This study investigated the effect of esculetin on collagen-induced human platelet aggregation, and we clarified the mechanism. Esuletin has effects on the down regulation of PI3K/Akt and MAPK, phosphoproteins that act in the signaling process in platelet aggregation. The effects of esculetin reduced of TXA2 production and phospholipase A2 activation, and intracellular granule secretion including ATP and serotonin, leading to inhibit platelet aggregation. These results clearly clarified the effect of esculetin in inhibiting platelet activity and thrombus formation in humans.

KCI등재SCOUPUS

2참가죽그물바탕말 1,9-Dihydroxycrenulide 및 Epiloliolide의 양모 효능

저자 : 강정일 ( Jung-il Kang ) , 오태헌 ( Tae Heon Oh ) , 김지오 ( Jioh Kim ) , 노해민 ( Haemin No ) , 이남호 ( Nam Ho Lee ) , 유은숙 ( Eun-sook Yoo ) , 강희경 ( Hee-kyoung Kang )

발행기관 : 한국생약학회 간행물 : 생약학회지 52권 3호 발행 연도 : 2021 페이지 : pp. 134-142 (9 pages)

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This study was conducted to evaluate the effects of Dictyota coriacea extract and its active components such as 1,9- dihydroxycrenulide and epiloliolide on the hair growth. Treatment with D. coriacea extract and the hexane and EtOAc fractions of D. coriacea extract significantly increased the proliferation of dermal papilla cells (DPCs), a central regulator of the hair cycle. Especially, 1,9-dihydroxycrenulide and epiloliolide from D. coriacea extract, caused an increase in the DPC proliferation. When isolated rat vibrissa follicles were treated with 1,9-dihydroxycrenulide or epiloliolide for 21 d, the hair-fiber lengths for the vibrissa follicles increased. When examined the activity of 5α-reductase, which converts testosterone to dihydrotestosterone (DHT), a main cause of androgenetic alopecia, the several solvent fractions of D. coriacea extract significantly decreased the 5α-reductase activity while 1,9-dihydroxycrenulide and epiloliolide scarcely inhibited 5α-reductase activity. In addition, we found that the D. coriacea extract and several solvent fractions of D. coriacea extract could not act as a KATP channel opener, which could be a contributory factor in the effect on hair growth. These results suggest that D. coriacea extract and 1,9-dihydroxycrenulide and epiloliolide, principals of D. coriacea, have the potential to treat alopecia via the proliferation of DPCs.

KCI등재SCOUPUS

3거품돌산호 추출물의 멜라닌 합성 억제 효능

저자 : 심중현 ( Joong Hyun Shim )

발행기관 : 한국생약학회 간행물 : 생약학회지 52권 3호 발행 연도 : 2021 페이지 : pp. 143-148 (6 pages)

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This study was performed to elucidate the inhibitory effects of Alveopora japonica extract on melanin synthesis by measuring the levels of cell viability, mRNA expression, tyrosinase activity, and melanin production in the B16F10 cell line. The effects of A. japonica extract on tyrosinase-related protein 1 (TYRP1), TYRP2, tyrosinase (TYR), and microphthalmia-associated transcription factor (MITF) mRNA expression levels and melanin content were determined. Quantitative real-time RTPCR show that A. japonica extract decrease the mRNA expression levels of TYRP1, TYRP2, TYR, and MITF in B16F10 cell line, resulting in lower levels of melanin production compared to α-MSH-treated B16F10 cells. Tyrosinase activity assays reveal that A. japonica extract decrease melanin production in B16F10 cells. These results demonstrate the whitening effects of A. japonica extract on B16F10 cells; thus, A. japonica extract is a potent ingredient for skin whitening. Further research is needed on the mechanism of action of A. japonica extract. Such research will benefit not only cosmetics, but also the health food and medical industries.

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4스피루리나 에탄올 추출물의 신경세포 보호활성

저자 : 류가희 ( Gahee Ryu ) , 마충제 ( Choong Je Ma )

발행기관 : 한국생약학회 간행물 : 생약학회지 52권 3호 발행 연도 : 2021 페이지 : pp. 149-156 (8 pages)

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Excessive glutamate can cause oxidative stress in neuronal cells and this can be the reason for neurodegenerative disease. In this study, we investigated the protective effect of Spirulina maxima hot ethanol extract on mouse hippocampal HT22 cell of which glutamate receptor has no function. HT22 cells were pre-treated with S. maxima sample at a dose dependent manner (1, 10 and 100 μg/ml). After an hour, glutamate was treated. Cell viability, reactive oxygen species (ROS) accumulation, Ca 2+ influx, decrease of mitochondrial membrane potential level and glutathione related assays were followed by then. S. maxima ethanol extract improved the cell viability by suppressing the ROS and Ca 2+ formation, retaining the mitochondrial membrane potential level and protecting the activity of the antioxidant enzymes compared with group of vehicle-treated controls. These suggest that S. maxima may decelerate the neurodegeneration by attenuating neuronal damage and oxidative stress.

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5마이크로웨이브 추출방법을 이용한 작약의 유효성분 추출 및 생리활성 측정

저자 : 이효진 ( Hyojin Lee ) , 장경원 ( Kyoung Won Jang )

발행기관 : 한국생약학회 간행물 : 생약학회지 52권 3호 발행 연도 : 2021 페이지 : pp. 157-162 (6 pages)

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The heat-mediated reflux apparatus extraction of Paeonia lactiflora Pall. has been widely used as a traditional extraction method. In this paper, the microwave apparatus extraction method of Paeonia Radix was performed and the active ingredients and functional constituents were compared with the reference extraction method. The most effective extraction condition of albiflorin using the microwave was 120℃ in 50% methanol, and paeoniflorin was maximally extracted at 60℃. The reduced level of paeoniflorin molecule at high-pressure and high-temperature extraction condition was caused by the molecular instability. Additionally, the microwave extraction of 50% methanol extracts at 150℃ showed the highest functional constituents determined by in vitro DPPH radical scavenging activity, polyphenol concentration, and tyrosinase inhibition assay. The microwave apparatus was adapted as a rapid, low-cost, and environmentally friendly method to extract active ingredients and the practical extraction conditions of Paeonia Radix can be used in industrial applications.

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6구증구포 맥문동 Ethyl acetate 분획물의 예쁜 꼬마선충 내의 항산화 효과

저자 : 권강무 ( Kang Mu Kwon ) , 김준형 ( Jun Hyeong Kim ) , 양재헌 ( Jae Heon Yang ) , 기별희 ( Byeolhui Ki ) , 황인현 ( In Hyun Hwang ) , 김대근 ( Dae Keun Kim )

발행기관 : 한국생약학회 간행물 : 생약학회지 52권 3호 발행 연도 : 2021 페이지 : pp. 163-169 (7 pages)

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Using the Caenorhabditis elegans model system, the antioxidant activity of methanol extract of the guzeunggupoprocessed Liriope platyphylla F. T. Wang (Liliaceae) tuber was calculated. Between the methanol extracts of guzeunggupo-processed and non-processed L. platyphylla tuber, the processed L. platyphylla tuber showed higher DPPH radical scavenging effect than the non-processed one. The ethyl acetate soluble fraction of the methanol extract of the guzeunggupo-processed L. platyphylla tuber showed the best DPPH radical scavenging activity. The ethyl acetate fraction of the processed sample was measured for the activities of superoxide dismutase (SOD), catalase, and oxidative stress tolerance by using C. elegans along with reactive oxygen species level. In addition, to verify the regulation of the stress response gene is responsible for the increased stress tolerance of C. elegans treated by the ethyl acetate fraction of the processed sample, SOD-3 expression was measured using a transgenic strain (CF1553). Consequently, the ethyl acetate fraction of the processed sample, increased SOD and catalase activities, and decreased ROS accumulation in a dose-dependent manner. Furthermore, the ethyl acetate fraction of the processed sample-treated CF1553 worm showed higher SOD-3::GFP intensity than the control worm.

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7Cyclophosphamide에 의해 유도된 면역 저하 마우스에서 흑미 (Oryza sativa L.) 호분층의 면역 활성 효과

저자 : 이은별 ( Eun Byeol Lee ) , 최지혜 ( Ji-hye Choi ) , 김시현 ( Si Hyun Kim ) , 장환희 ( Hwan Hee Jang ) , 이성현 ( Sung Hyen Lee )

발행기관 : 한국생약학회 간행물 : 생약학회지 52권 3호 발행 연도 : 2021 페이지 : pp. 170-176 (7 pages)

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Anthocyanins are considered as one of the major functional components including cyanidin-3-glucoside and peonidin- 3-glucoside anthocyanins of black rice (Oryza sativa L., BR) aleurone layer (OAL) extract. The immunomodulatory biological effects of OAL has rarely been evaluated. This study has extensively studied the immunomodulatory effects of BR in immunosuppressed mice by CPA. The immunostimulatory effects of BR extract in in vivo at 150 (OAL-1) and 300 mg/kg body weight (OAL-2) for OAL groups were measured and compared to the normal group (CON) ingested with distilled water only or other groups. After the 14 days of oral administration, immune related organ weights, serum immunoglobulin (Ig) A and IgG, and cytokines (IL-12, TNF-α) levels, splenocytes proliferation rate, and NK cell activity were evaluated as immune related biomarkers. The serum IgA and IgG values in the OAL-treated groups increased to the level of the CON. OAL significantly and effectively controlled the IL-12 and TNF-α levels and NK activity compared to the negative control (NC) group. The results suggest that BR aleurone layer with immunomodulatory activities has a great potential as a functional food by itself or as a supplement.

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8만성 역류성 식도염에서 황련과 오수유 혼합물이 식도 점막에 미치는 영향

저자 : 이진아 ( Jin A Lee ) , 오민혁 ( Min Hyuck Oh ) , 신미래 ( Mi-rae Shin ) , 노성수 ( Seong-soo Roh ) , 박해진 ( Hae-jin Park )

발행기관 : 한국생약학회 간행물 : 생약학회지 52권 3호 발행 연도 : 2021 페이지 : pp. 177-185 (9 pages)

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Gastroesophageal reflux disease (GERD) is a gastrointestinal disorder in which stomach contents reflux into the esophagus, causing complications such as mucosal damage. The purpose of this study was to determine the effect of Rhei Rhizoma and Scutellariae Radix mixture (RS) in chronic acid reflux esophagitis (CARE), one of the GERD. After inducing reflux esophagitis through surgery, the group was separated and the drug was administered for 2 weeks; Normal rats (Normal, n=8), chronic acid reflux esophagitis rats (Control, n=8), tocopherol 30 mg/kg-treated chronic acid reflux esophagitis rats (Toco, n=8), Rhei Rhizoma and Scutellariae Radix mixture 100 mg/kg-treated chronic acid reflux esophagitis rats (RSL, n=8), Rhei Rhizoma and Scutellariae Radix mixture 200 mg/kg-treated chronic acid reflux esophagitis rats (RSH, n=8). Gross lesion of esophageal mucosa after RS treatment showed a superior enhancement compared with that of Control group. Additionally, RS significantly decreased the levels of MPO and MDA, effectively inhibited NADPH oxidase, and regulated the expression of the AMPK/ LKB1/NF-κB pathway. Moreover, it significantly increased the expression of tight junction proteins. Taken together, RS not only alleviates inflammation of the esophageal mucosa via the AMPK/LKB1/NF-κB pathway by reducing oxidative stress, but also improves esophageal function by modulating tight junction proteins.

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9곰보배추에 함유된 6-Hydroxyluteolin 7-O-Glucoside 분석을 위한 HPLC/DAD 분석법 밸리데이션

저자 : 이학동 ( Hak-dong Lee ) , 바헤 ( Leo Adrianne Paje ) , 최중원 ( Jungwon Choi ) , 김주리 ( Juree Kim ) , 유아람 ( A Ram Yu ) , 배민정 ( Min-jung Bae ) , 이상현 ( Sanghyun Lee )

발행기관 : 한국생약학회 간행물 : 생약학회지 52권 3호 발행 연도 : 2021 페이지 : pp. 186-191 (6 pages)

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We developed a method to identify and quantify 6-hydroxyluteolin 7-O-glucoside in the powder of Salvia plebeia (PS) using high-performance liquid chromatography coupled with diode array detector (HPLC/DAD) and equipped with reverse-phase INNO C18 column. The analytical method was optimized and validated using novel parameters. The obtained values for the limits of detection and quantification were 3.60 and 10.90 μg/mL, respectively. Calibration curve showed good linearity in the concentration range tested (0.00625-0.1 mg/mL, r 2 = 1.0000), high accuracy (96.2-101.4%), and precision values (RSD ≤ 0.27%). Our analysis support the use of our method for accurately identifying and quantifying 6-hydroxyluteolin 7-Oglucoside from PS in routine analyses and large-scale extraction processes for content determination.

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10제조방법에 따른 당귀수산(當歸鬚散)의 성분분석 및 항염증 효과

저자 : 전영희 ( Young-hee Jeon ) , 남원희 ( Won-hee Nam ) , 임현희 ( Hyun-hee Leem ) , 김세진 ( Se-jin Kim ) , 유병우 ( Byung-woo Yu ) , 손수미 ( Su-mi Son ) , 김명진 ( Myoung-jin Kim ) , 최혜민 ( Hye-min Choi ) , 권현숙 ( Hyun-sook Kwon ) , 김정옥 ( Jung-ok Kim )

발행기관 : 한국생약학회 간행물 : 생약학회지 52권 3호 발행 연도 : 2021 페이지 : pp. 192-201 (10 pages)

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Dangguisu-san (DGSS) is widely known traditional herbal medicinal formula in Korea for treatment of traumatic injury by traffic accident, ecchymosis, abdominal distension and anti-thrombosis of blood. This study was conducted to develop the simultaneous analyze method using high performance liquid chromatography (HPLC) and examine the effect of anti-inflammatory activity of DGSS-dry extract (DGSS-DE) and DGSS-mix extract powder (DGSS-MEP). Physicochemical characteristics of DGSS-DE and DGSS-MEP showed that there is no significant difference in pH, titratable acidity, total soluble solid content and browning degree except for color value (L, a, b). 15 functional constituents of DGSS were identified and the correlation coefficient values of DGSS-DE and DGSS-MEP were conformed 0.950. Also, DGSS-DE and DGSS-MEP significantly decreased the secretion of nitric oxide (NO), prostaglandin E2 (PGE2), interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) through inhibited expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), IL-1β, IL-6, and TNF-α. From these result, DGSS-MEP showed similar chemical composition and anti-inflammatory effect to DGSS-DE. Therefore, DGSS-DE and DGSS-MEP may be useful as potential source of drug to prevent inflammation.

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