논문 상세보기

한국어병학회> 한국어병학회지> Infection status and microhabitat of polyopisthocotylean Monogenea found on the gills of goldlined seabream, Rhabdosargus sarba, from Musairah Island, Oman

KCI후보

Infection status and microhabitat of polyopisthocotylean Monogenea found on the gills of goldlined seabream, Rhabdosargus sarba, from Musairah Island, Oman

Maryam Al Sawafi , Miyoung Cho , Adil Al Sulaimani , Gilha Yoon
  • : 한국어병학회
  • : 한국어병학회지 34권1호
  • : 연속간행물
  • : 2021년 06월
  • : 55-61(7pages)
한국어병학회지

DOI


목차

Introduction
Materials and Methods
Results
Discussion
Conclusion
Acknowledgements
References

키워드 보기


초록 보기

One of the major problems in fish production is that parasite-induced episodes of disease can result in significant impacts to the health of stock, mortalities and, economical losses. Seabreams are economically important fish in both the capture fishery and aquaculture sectors in Oman and represent potential species for aquaculture. The current study set out to investigate infection of polyopisthocotylean monogeneans on the gills of wild goldlined seabream, Rhabdosargus sarba, caught from Musairah region in Oman. The prevalence, mean intensity and abundance of polyopisthocotyleans on 145 fish was found to be 52.41%, 5.65 and 3.04, respectively. Three species of microcotylid parasite were isolated, one being Atrispinum acarne while the remaining two species require further study to identify them. There was no significant difference in the infection rate between host sex, however, parasite burdens were higher on larger sized fish (p<0.05) and were found to favour the first gill arch (p< 0.05). The study found that the sample of Rhabdosargus sarba landed in the waters around Musairah are heavily infected, with a predominantly immature population of microcotylids. Further work is required to better understand the potential risk each species poses to the health of its host before a decision is made on the suitability of new candidate fish species for commercial aquaculture.

UCI(KEPA)

간행물정보

  • : 수해양분야  > 수산생물학
  • : KCI후보
  • :
  • : 반년간
  • : 1226-0819
  • : 2233-5412
  • : 학술지
  • : 연속간행물
  • : 1988-2021
  • : 816


저작권 안내

한국학술정보㈜의 모든 학술 자료는 각 학회 및 기관과 저작권 계약을 통해 제공하고 있습니다.

이에 본 자료를 상업적 이용, 무단 배포 등 불법적으로 이용할 시에는 저작권법 및 관계법령에 따른 책임을 질 수 있습니다.

34권1호(2021년 06월) 수록논문
최근 권호 논문
| | | |

KCI후보

1Experimental transmission of red sea bream iridovirus (RSIV) between rock bream (Oplegnathus fasciatus) and rockfish (Sebastes schlegelii)

저자 : Joon Gyu Min , Ye Jin Jeong , Min A Jeong , Jae-ok Kim , Jee Youn Hwang , Mun-gyeong Kwon , Kwang Il Kim

발행기관 : 한국어병학회 간행물 : 한국어병학회지 34권 1호 발행 연도 : 2021 페이지 : pp. 1-7 (7 pages)

다운로드

(기관인증 필요)

초록보기

Red sea bream iridovirus (RSIV), belonging to the genus Megalocytivirus, is the predominant cause of mortality in marine fishes in Korea, including rock bream (Oplegnathus fasciatus). Rockfish (Sebastes schlegelii) are the host fish for RSIV, exhibiting no clinical signs or mortality. Cohabitation challenges, which mimicked natural transmission conditions, were performed to evaluate viral transmission between rock bream and rockfish, and to determine the pathogenicity and viral loads. In cohabitation challenge, artificially RSIV-infected rock bream were the viral donor, and healthy rockfish were the recipient. The results showed that although the donor rock bream had 95-100 % cumulative mortality (>108 viral genome copies/mg of spleen 7-14 days after viral infection), the recipient rockfish did not die, even when the viral genome copies in the spleen were >105 copies/mg. These results indicated asymptomatic infections. Notably, in a reverse-cohabitation challenge (artificially RSIV-infected rockfish as the viral donor and healthy rock bream as the recipient), RSIV horizontally infected from subclinical rockfish to rock bream (107 viral genome copies/mg of spleen 21 days after cohabitation) with 10-20% cumulative mortality. These results suggest that an asymptomatic, infected rockfish can naturally transmit the RSIV without being sacrificed.

KCI후보

2Microsatellite marker distribution pattern in rock bream iridovirus (RBIV) infected rock bream, Oplegnathus fasciatus

저자 : Myung-hwa Jung , Sung-ju Jung

발행기관 : 한국어병학회 간행물 : 한국어병학회지 34권 1호 발행 연도 : 2021 페이지 : pp. 9-15 (7 pages)

다운로드

(기관인증 필요)

초록보기

Rock bream (Oplegnathus fasciatus) is a highly valued aquaculture species in Korea. However, the aquaculture industry suffers huge economic losses due to rock bream iridovirus (RBIV) infection in summer. The objective of this study was to determine genetic diversity and relationships of DNAs isolated from two groups of rock bream after RBIV infection using five microsatellite (MS) markers. The first group of fish died early and the second group of fish died later after RBIV infection. In this experiment, 90 fish (5.1±1.0 cm and 4.1±1.3 g) were injected with 50 μl of RBIV (104 TCID50/ml) and maintained at 26°C for 15 days. Genomic DNAs were extracted from fins of 20 fish that died earlier or later after RBIV infection. These DNAs were subjected to genotyping using five MS markers (CA-03, CA3-05, CA3-06, CA-10, and CA3-36). Of these markers, CA3-05 (early death group), CA3-06 (late death group), and CA3-36 (both early and late death groups) showed different alleles distribution rates. In-depth studies are needed to provide valuable information for selecting RBIV-resistant fish. In conclusion, microsatellite marker distribution pattern differences between early- and late- death groups of rock bream after RBIV infection showing different RBIV susceptibilities were determined using MS markers and genotyping. Results of this study suggest that MS markers could be used to facilitate the selection of RBIV resistant rock bream.

KCI후보

3Marine birnavirus (MABV)'s 5´ terminal region of segment A acts as internal ribosome entry site (IRES)

저자 : So Yeon Kim , Ki Hong Kim

발행기관 : 한국어병학회 간행물 : 한국어병학회지 34권 1호 발행 연도 : 2021 페이지 : pp. 17-22 (6 pages)

다운로드

(기관인증 필요)

초록보기

Eukaryotic translation is initiated by either cap-dependent or cap-independent way, and the capindependent translation can be initiated by the internal ribosomal entry site (IRES). In this study, to know whether the 5'UTR leader sequence of marine birnavirus (MABV) segment A and segment B can act as IRES, bicistronic vectors harboring a CMV promoter-driven red fluorescent gene (mCherry) and poliovirus IRES- or MABV's leader sequence-driven green fluorescent gene (eGFP) were constructed, then, transfected into a mammalian cell line (BHK-21 cells) and a fish cell line (CHSE-214 cells). The results showed that the poliovirus IRES worked well in BHK-21 cells, but did not work in CHSE-214 cells. In the evaluation of MABV's leader sequences, the reporter eGFP gene under the 5'UTR leader sequence of MABV's segment A was well-translated in CHSE-214 cells, indicating 5'UTR of MABV's segment A initiates translation in the cap-independent way and can be used as a fish-specific IRES system. However, the 5'UTR leader sequence of MABV's segment B did not initiate translation in CHSE-214 cells. As the precise mechanism of birnavirid IRES-mediated translation is not known, more elaborate investigations are needed to uncover why the leader sequence of segment B could not initiate translation in the present study. In addition, further studies on the host species range of MABV's segment A IRES and on the screening of other fish-specific IRESs are needed.

KCI후보

4제브라피쉬 interferon regulatory factor 10의 주사에 따른 면역 유전자 발현과 VHSV에 대한 방어 효과

저자 : 김혜지 ( Hye Ji Kim ) , 김진영 ( Jin Young Kim ) , 박종빈 ( Jong Bin Park ) , 이지현 ( Ji Hyun Lee ) , 박정수 ( Jeong Su Park ) , 김형준 ( Hyoung Jun Kim ) , 권세련 ( Se Ryun Kwon )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 34권 1호 발행 연도 : 2021 페이지 : pp. 23-29 (7 pages)

다운로드

(기관인증 필요)

초록보기

Interferon regulatory factors (IRFs) are a family of transcription factors essential to the control of antiviral immune response, cell growth, differentiation and apoptosis. IRF10 of zebrafish (Danio rerio) was negative regulation of the interferonΦ1 and 3 response in vitro. In this study, we analyze the induction of in vivo immune response activation from the IRF10 gene of zebrafish and the protective effect against VHSV. As the results, the group inoculated with IRF10 expression vectors, there was no expression of IFNΦ1, suggestion that IRF10 may function as a negative regulator of IRF3, which binds to the IFNΦ1 promoter. And other types of interferon genes (IFNΦ2-4) are thought to have been activated, inducing to the expression of pro-inflammatory cytokine and Mx genes. As the results of challenge test performed at 14 days after inoculation of the expression vectors, the maximum survival rate [50% (1μg DNA) and 42.5% (10μg DNA)] for IRF10 group were recorded. Meanwhile, the survival rates of pcDNA3.1 and PBS as the control groups were 10% and 15%, respectively. This study suggests that the possibility that activation of IRF10 molecule could be exploited as a VHS control method.

KCI후보

5양식넙치와 뱀장어에서 분리된 Edwardsiella tarda의 특성 비교

저자 : 김은희 ( Eunheui Kim )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 34권 1호 발행 연도 : 2021 페이지 : pp. 31-38 (8 pages)

다운로드

(기관인증 필요)

초록보기

본 연구는 1996년부터 2010년 사이에 우리나라 양식넙치와 뱀장어에서 분리하여 Edwardsiella tarda로 동정하였던 18 균주에 대하여 생화학적 특성과 RAPD profile을 비교해봄으로써 이전 양식생물의 질병에 관여되었던 Edwardsiella속 세균의 다양성을 알아보고자 하였다. 생화학적 특성으로 볼 때 이들은 citrate 분해, H2S 그리고 indole 생산 결과에 차이를 보여 4가지 패턴으로 구분되었으나 모두 E. tarda로 동정되었고 숙주에 따른 분리균의 특성으로 구분되지는 않았다. E. tarda 특이 primer인 EDtT로 PCR을 실시한 결과 18 분리균에서는 모두 약 270 bp의 동일한 band가 검출되었으나 비교 균주로 사용된 E. tarda와 E. ictaluri의 type strain에서는 특이 밴드가 검출되지 않았다. 또한 Ready-To-Go-RAPD kit의 primer 5번과 6번으로 실시한 RAPD PCR 결과, 넙치 분리균, 뱀장어 분리균, 그리고 E. tarda와 E. ictaluri type strain에서 band profile이 뚜렷한 차이를 보여 origin에 따른 특징으로 정리될 수 있었으며 병원체 모니터링을 위한 tool의 하나로 개발될 가능성을 보였다.


The objective of this study was to determine comparative biochemical characteristics and RAPD (random amplified polymorphic DNA) profiles of 18 strains of Edwardsiella tarda isolated from cultured olive flounder (Paralichthys olivaceus) and eel (Anguilla spp) that showed diseases between 1996 and 2010 in Korea. In terms of biochemical properties, they showed four patterns with differences in citrate degradation and production of H2S and indole. All strains were identified as E. tarda. Characteristics of isolates were not classified according to their host. As a result of PCR with E. tarda-specific primer, EDtT, the same band of about 270 bp was detected in all 18 isolates. However, no specific band was detected in type strains of E. tarda or Edwardsiella ictaluri. As a result of RAPD PCR performed with primer No. 5 and No. 6 of a Ready-To-Go-RAPD kit, the band profile showed clear differences among isolates of olive flounder, isolates of eel, and E. tarda and E. ictaluri type strains. It was possible to organize their characteristics according to the origin of host with possibility to develop tools for pathogen monitoring.

KCI후보

6경기도 내 양식어류에서 분리한 병원성 세균의 Tetracycline 내성 유전자 분포

저자 : 조기택 ( Ki-taek Cho ) , 황윤정 ( Yun-jeong Hwang ) , 이상우 ( Sang-woo Lee ) , 김광일 ( Kwang-il Kim ) , 정현도 ( Hyun-do Jeong )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 34권 1호 발행 연도 : 2021 페이지 : pp. 39-46 (8 pages)

다운로드

(기관인증 필요)

초록보기

Tetracycline (TC)은 국내에서 세균 감염 치료에 반드시 필요한 대표적인 항생제로 알려져 있다. 그러나 부적절한 사용과 남용으로 인해 Tetracycline, Erythromycin, Fluoroquinolone 등과 같은 항생제에 대한 내성이 발생하고 있으며, 이는 국내 양식 분야에서 심각한 경제적 피해를 유발한다. 본 연구에서는 2015~2018년에 걸쳐 경기도 양식장의 양식생물에서 101개 균주를 분리하였다. 분리균주는 간이적인 생화학적 방법을 통해 동정하였으며, 최소 억제농도(MIC)의 확인을 통해 Oxytetracycline (OTC), Ampicillin (AMP), Clindamycin (CLI), Enrofloxacin (ENRO), Gentamycin (GEN)에 대한 내성 여부를 확인하였다. 이중 TC에 내성을 보이는 균주는 PCR법을 통해 tet 유전자의 분포를 조사하였다. 그 결과, 총 101개 균주 중에서 Aeromonas spp. 가 44개(43.5%)로 가장 우점하였고, 그 다음으로 Pseudomonas spp. 4개(4.0%), Vibrio spp. 5개(5.0%)가 확인되었다. 또한, 다중 내성을 보이는 균주(77.2%)가 단일 내성균(22.8%)보다 많음을 확인하였다. tet(A), tet(D), tet(E), tet(G), tet(M), tet(S)가 TC 내성 균주에서 검출되었으며 tet(A)가 가장 우점적으로 확인되었다. Aeromonas spp.는 분리된 균주 중에서 가장 많았으며, 경기도 내 양식현장에서의 다양한 항생제 내성 유전자의 특성에 대한 추가적인 연구가 필요하다.


Tetracycline (TC) is one of the antibiotics used for treatment of bacterial infection in Korea. Inadequate usage and abuse cause the resistance to antibiotics, like Tetracycline, Erythromycin, and Fluoroquinolone. It can also affect severe economic loss in aquaculture field in Korea. We isolated 101 bacterial samples from diseased fish at aquaculture sites in Gyeonggi-do during 2015~2018. Minimum inhibitory concentration (MIC) method has been used to determine distribution and to identify bacterial isolates resistant to antibiotics including Oxytetracycline (OTC), Ampicillin (AMP), Clindamycin (CLI), Enrofloxacin (ENRO), Gentamycin (GEN). TC resistant isolates were confirmed antibiotic resistant genes by conventional PCR. Bacterial isolates were identified as Aeromonas spp. (43.5%), Pseudomonas spp. (4.0%) and Vibrio spp. (5.0%). It was confirmed that multi-resistant isolates (77.2%) were predominant over single-resistant one (22.8%). TC resistant genes like tet(A), tet(D), tet(E), tet(G), tet(M), and tet(S) were detected and tet(A) was the most prevalent. Aeromonas spp. is a dominant strain in bacterial infections in fishes of aquaculture sites, and further investigation on various antibiotic resistance genes will be needed for clear understanding of aquaculture sites in Gyeonggi-do.

KCI후보

7First report of Paranophrys marina (Protozoa, Ciliophora, Scuticociliatia) isolated from olive flounder Paralichthys olivaceus in Korea: morphological and phylogenetic analysis

저자 : Hyun-sil Kang , Ilson Whang , Jae-kwon Cho

발행기관 : 한국어병학회 간행물 : 한국어병학회지 34권 1호 발행 연도 : 2021 페이지 : pp. 47-53 (7 pages)

다운로드

(기관인증 필요)

초록보기

Scuticociliates are one of the serious parasitic threats faced by the marine aquaculturists worldwide. To date, Uronema nigricans, Philasterides dicentrarchi, Miamiensis avidus, Uronema marinum, and Pseudocohnilembus persalinus have been reported as the important culprit species causing scuticociliatosis in fish. The present paper reports the finding of an additional scuticociliate isolate from the gill of diseased olive flounder Paralichthys olivaceus in Korea. Based on the morphological characteristics, a scuticociliate in this study was identified as Paranophrys marina. Phylogenetic analysis placed P. marina as a sister lineage to three species of Pseudocohnilembus and Mesanophrys carcini within the order Philasterida.

KCI후보

8Infection status and microhabitat of polyopisthocotylean Monogenea found on the gills of goldlined seabream, Rhabdosargus sarba, from Musairah Island, Oman

저자 : Maryam Al Sawafi , Miyoung Cho , Adil Al Sulaimani , Gilha Yoon

발행기관 : 한국어병학회 간행물 : 한국어병학회지 34권 1호 발행 연도 : 2021 페이지 : pp. 55-61 (7 pages)

다운로드

(기관인증 필요)

초록보기

One of the major problems in fish production is that parasite-induced episodes of disease can result in significant impacts to the health of stock, mortalities and, economical losses. Seabreams are economically important fish in both the capture fishery and aquaculture sectors in Oman and represent potential species for aquaculture. The current study set out to investigate infection of polyopisthocotylean monogeneans on the gills of wild goldlined seabream, Rhabdosargus sarba, caught from Musairah region in Oman. The prevalence, mean intensity and abundance of polyopisthocotyleans on 145 fish was found to be 52.41%, 5.65 and 3.04, respectively. Three species of microcotylid parasite were isolated, one being Atrispinum acarne while the remaining two species require further study to identify them. There was no significant difference in the infection rate between host sex, however, parasite burdens were higher on larger sized fish (p<0.05) and were found to favour the first gill arch (p< 0.05). The study found that the sample of Rhabdosargus sarba landed in the waters around Musairah are heavily infected, with a predominantly immature population of microcotylids. Further work is required to better understand the potential risk each species poses to the health of its host before a decision is made on the suitability of new candidate fish species for commercial aquaculture.

KCI후보

9멍게 물렁증 근육변성의 병인에 관한 연구

저자 : 허민도 ( Min Do Huh ) , 이효은 ( Hyo Eun Lee ) , 이무근 ( Mu Kun Lee ) , 김보성 ( Bo Sung Kim )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 34권 1호 발행 연도 : 2021 페이지 : pp. 63-70 (8 pages)

다운로드

(기관인증 필요)

초록보기

As a part of research to elucidate the pathogenesis of so called Soft Tunic Syndrome(STS), that caused mass mortalities in the cultured sea squirts, Halocynthia roretzi, the epidemiological and pathological analysis were done to both clinically normal and diseased groups of the farms of Tongyoung and Geoje coastal areas in southeast sea from February to July, 2008. In the histological finding of the tunic, most of individuals showed tunic softness syndromes that included the disarrangement and destruction of tunic fiber with the simultaneous presence of flagellates-like cells, recently suspected as main agents of tunic softness syndromes. Simultaneously, the intensive degenerative changes of the skeletal muscle of diseased sea squirts were recognized. The changes were characterized with the hyalinization and condensation of muscle fibril and hemocytic infiltration in the muscle fibers. Those were thought to be a kind of typical Zenker's necrosis as in the skeletal muscle of higher vertebrates. Besides of the diseased sea squirts, Zenker's necrosis of skeletal muscles were seen in the normal ones. Epidemiological inquiry for diseased groups revealed that the higher incidences of tunic softness syndrome were recorded in the fast growing groups and in the sites presuming the organic pollution. And Higher malondialadehyde(MDA) and glutathione peroxidase(GPx) activity were detected in the groups showing STS. Those results suggested that Zenker's necrosis of body muscles was a kind of“nutritional myopathy" by oxidative stress. Conclusively, it was considered that Zenker's necrosis of body muscles gives an important clue for elucidating pathogenesis of STS of cultured squirts. And it seems that the necrosis were caused by the oxidative stress to body muscle during abnormal rapid growth of sea squirts.

KCI후보

10흰다리새우(Litopenaeus vannamei)에서 cephalexin의 잔류농도 연구를 위한 LC-MS/MS 분석법 연구

저자 : 양찬영 ( Chan Yeong Yang ) , 배준성 ( Jun Sung Bae ) , 이채원 ( Chae Won Lee ) , 정은하 ( Eun Ha Jeong ) , 이지훈 ( Ji-hoon Lee ) , 박수진 ( Su-jin Bak ) , 최상훈 ( Sang-hoon Choi ) , 박관하 ( Kwan Ha Park )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 34권 1호 발행 연도 : 2021 페이지 : pp. 71-80 (10 pages)

다운로드

(기관인증 필요)

초록보기

Cephalexin, a semi-synthetic cephalosporin antibiotic, has long been used in fish aquaculture in various countries under legal authorization. The drug is thus widely available for use in other aquatic species except fishes like the crustacean whiteleg shrimp. This study aims to develop a sensitive method for laboratory residue studies to adopt in withdrawal period determinations. Through repeated trials from the existing methods developed for other food animal tissues, it was possible to achieve a sensitive high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method. The results showed that at a concentration of 0.1 mg/kg, the recovery rate was 81.79%, and C.V. value was 8.2%, which meet the recovery rate and C.V. recommended by Codex guideline. After satisfactory validation of analytical procedures, applicability to the shrimp tissue was confirmed in experimentally cephalexin-treated whiteleg shrimp. As a result, most muscle samples were detected below the limit of quantification (0.05 mg/kg) after day 3, and most hepatopancreas samples were detected below the limit of quantification after day 14. In particular, the limit of quantification 0.05 ppm with the presently developed method suggests sufficient sensitive over the current legal maximum residue limit of 0.2 mg/kg set for fishes.

12
권호별 보기
같은 권호 다른 논문
| | | | 다운로드

KCI후보

1Experimental transmission of red sea bream iridovirus (RSIV) between rock bream (Oplegnathus fasciatus) and rockfish (Sebastes schlegelii)

저자 : Joon Gyu Min , Ye Jin Jeong , Min A Jeong , Jae-ok Kim , Jee Youn Hwang , Mun-gyeong Kwon , Kwang Il Kim

발행기관 : 한국어병학회 간행물 : 한국어병학회지 34권 1호 발행 연도 : 2021 페이지 : pp. 1-7 (7 pages)

다운로드

(기관인증 필요)

초록보기

Red sea bream iridovirus (RSIV), belonging to the genus Megalocytivirus, is the predominant cause of mortality in marine fishes in Korea, including rock bream (Oplegnathus fasciatus). Rockfish (Sebastes schlegelii) are the host fish for RSIV, exhibiting no clinical signs or mortality. Cohabitation challenges, which mimicked natural transmission conditions, were performed to evaluate viral transmission between rock bream and rockfish, and to determine the pathogenicity and viral loads. In cohabitation challenge, artificially RSIV-infected rock bream were the viral donor, and healthy rockfish were the recipient. The results showed that although the donor rock bream had 95-100 % cumulative mortality (>108 viral genome copies/mg of spleen 7-14 days after viral infection), the recipient rockfish did not die, even when the viral genome copies in the spleen were >105 copies/mg. These results indicated asymptomatic infections. Notably, in a reverse-cohabitation challenge (artificially RSIV-infected rockfish as the viral donor and healthy rock bream as the recipient), RSIV horizontally infected from subclinical rockfish to rock bream (107 viral genome copies/mg of spleen 21 days after cohabitation) with 10-20% cumulative mortality. These results suggest that an asymptomatic, infected rockfish can naturally transmit the RSIV without being sacrificed.

KCI후보

2Microsatellite marker distribution pattern in rock bream iridovirus (RBIV) infected rock bream, Oplegnathus fasciatus

저자 : Myung-hwa Jung , Sung-ju Jung

발행기관 : 한국어병학회 간행물 : 한국어병학회지 34권 1호 발행 연도 : 2021 페이지 : pp. 9-15 (7 pages)

다운로드

(기관인증 필요)

초록보기

Rock bream (Oplegnathus fasciatus) is a highly valued aquaculture species in Korea. However, the aquaculture industry suffers huge economic losses due to rock bream iridovirus (RBIV) infection in summer. The objective of this study was to determine genetic diversity and relationships of DNAs isolated from two groups of rock bream after RBIV infection using five microsatellite (MS) markers. The first group of fish died early and the second group of fish died later after RBIV infection. In this experiment, 90 fish (5.1±1.0 cm and 4.1±1.3 g) were injected with 50 μl of RBIV (104 TCID50/ml) and maintained at 26°C for 15 days. Genomic DNAs were extracted from fins of 20 fish that died earlier or later after RBIV infection. These DNAs were subjected to genotyping using five MS markers (CA-03, CA3-05, CA3-06, CA-10, and CA3-36). Of these markers, CA3-05 (early death group), CA3-06 (late death group), and CA3-36 (both early and late death groups) showed different alleles distribution rates. In-depth studies are needed to provide valuable information for selecting RBIV-resistant fish. In conclusion, microsatellite marker distribution pattern differences between early- and late- death groups of rock bream after RBIV infection showing different RBIV susceptibilities were determined using MS markers and genotyping. Results of this study suggest that MS markers could be used to facilitate the selection of RBIV resistant rock bream.

KCI후보

3Marine birnavirus (MABV)'s 5´ terminal region of segment A acts as internal ribosome entry site (IRES)

저자 : So Yeon Kim , Ki Hong Kim

발행기관 : 한국어병학회 간행물 : 한국어병학회지 34권 1호 발행 연도 : 2021 페이지 : pp. 17-22 (6 pages)

다운로드

(기관인증 필요)

초록보기

Eukaryotic translation is initiated by either cap-dependent or cap-independent way, and the capindependent translation can be initiated by the internal ribosomal entry site (IRES). In this study, to know whether the 5'UTR leader sequence of marine birnavirus (MABV) segment A and segment B can act as IRES, bicistronic vectors harboring a CMV promoter-driven red fluorescent gene (mCherry) and poliovirus IRES- or MABV's leader sequence-driven green fluorescent gene (eGFP) were constructed, then, transfected into a mammalian cell line (BHK-21 cells) and a fish cell line (CHSE-214 cells). The results showed that the poliovirus IRES worked well in BHK-21 cells, but did not work in CHSE-214 cells. In the evaluation of MABV's leader sequences, the reporter eGFP gene under the 5'UTR leader sequence of MABV's segment A was well-translated in CHSE-214 cells, indicating 5'UTR of MABV's segment A initiates translation in the cap-independent way and can be used as a fish-specific IRES system. However, the 5'UTR leader sequence of MABV's segment B did not initiate translation in CHSE-214 cells. As the precise mechanism of birnavirid IRES-mediated translation is not known, more elaborate investigations are needed to uncover why the leader sequence of segment B could not initiate translation in the present study. In addition, further studies on the host species range of MABV's segment A IRES and on the screening of other fish-specific IRESs are needed.

KCI후보

4제브라피쉬 interferon regulatory factor 10의 주사에 따른 면역 유전자 발현과 VHSV에 대한 방어 효과

저자 : 김혜지 ( Hye Ji Kim ) , 김진영 ( Jin Young Kim ) , 박종빈 ( Jong Bin Park ) , 이지현 ( Ji Hyun Lee ) , 박정수 ( Jeong Su Park ) , 김형준 ( Hyoung Jun Kim ) , 권세련 ( Se Ryun Kwon )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 34권 1호 발행 연도 : 2021 페이지 : pp. 23-29 (7 pages)

다운로드

(기관인증 필요)

초록보기

Interferon regulatory factors (IRFs) are a family of transcription factors essential to the control of antiviral immune response, cell growth, differentiation and apoptosis. IRF10 of zebrafish (Danio rerio) was negative regulation of the interferonΦ1 and 3 response in vitro. In this study, we analyze the induction of in vivo immune response activation from the IRF10 gene of zebrafish and the protective effect against VHSV. As the results, the group inoculated with IRF10 expression vectors, there was no expression of IFNΦ1, suggestion that IRF10 may function as a negative regulator of IRF3, which binds to the IFNΦ1 promoter. And other types of interferon genes (IFNΦ2-4) are thought to have been activated, inducing to the expression of pro-inflammatory cytokine and Mx genes. As the results of challenge test performed at 14 days after inoculation of the expression vectors, the maximum survival rate [50% (1μg DNA) and 42.5% (10μg DNA)] for IRF10 group were recorded. Meanwhile, the survival rates of pcDNA3.1 and PBS as the control groups were 10% and 15%, respectively. This study suggests that the possibility that activation of IRF10 molecule could be exploited as a VHS control method.

KCI후보

5양식넙치와 뱀장어에서 분리된 Edwardsiella tarda의 특성 비교

저자 : 김은희 ( Eunheui Kim )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 34권 1호 발행 연도 : 2021 페이지 : pp. 31-38 (8 pages)

다운로드

(기관인증 필요)

초록보기

본 연구는 1996년부터 2010년 사이에 우리나라 양식넙치와 뱀장어에서 분리하여 Edwardsiella tarda로 동정하였던 18 균주에 대하여 생화학적 특성과 RAPD profile을 비교해봄으로써 이전 양식생물의 질병에 관여되었던 Edwardsiella속 세균의 다양성을 알아보고자 하였다. 생화학적 특성으로 볼 때 이들은 citrate 분해, H2S 그리고 indole 생산 결과에 차이를 보여 4가지 패턴으로 구분되었으나 모두 E. tarda로 동정되었고 숙주에 따른 분리균의 특성으로 구분되지는 않았다. E. tarda 특이 primer인 EDtT로 PCR을 실시한 결과 18 분리균에서는 모두 약 270 bp의 동일한 band가 검출되었으나 비교 균주로 사용된 E. tarda와 E. ictaluri의 type strain에서는 특이 밴드가 검출되지 않았다. 또한 Ready-To-Go-RAPD kit의 primer 5번과 6번으로 실시한 RAPD PCR 결과, 넙치 분리균, 뱀장어 분리균, 그리고 E. tarda와 E. ictaluri type strain에서 band profile이 뚜렷한 차이를 보여 origin에 따른 특징으로 정리될 수 있었으며 병원체 모니터링을 위한 tool의 하나로 개발될 가능성을 보였다.

KCI후보

6경기도 내 양식어류에서 분리한 병원성 세균의 Tetracycline 내성 유전자 분포

저자 : 조기택 ( Ki-taek Cho ) , 황윤정 ( Yun-jeong Hwang ) , 이상우 ( Sang-woo Lee ) , 김광일 ( Kwang-il Kim ) , 정현도 ( Hyun-do Jeong )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 34권 1호 발행 연도 : 2021 페이지 : pp. 39-46 (8 pages)

다운로드

(기관인증 필요)

초록보기

Tetracycline (TC)은 국내에서 세균 감염 치료에 반드시 필요한 대표적인 항생제로 알려져 있다. 그러나 부적절한 사용과 남용으로 인해 Tetracycline, Erythromycin, Fluoroquinolone 등과 같은 항생제에 대한 내성이 발생하고 있으며, 이는 국내 양식 분야에서 심각한 경제적 피해를 유발한다. 본 연구에서는 2015~2018년에 걸쳐 경기도 양식장의 양식생물에서 101개 균주를 분리하였다. 분리균주는 간이적인 생화학적 방법을 통해 동정하였으며, 최소 억제농도(MIC)의 확인을 통해 Oxytetracycline (OTC), Ampicillin (AMP), Clindamycin (CLI), Enrofloxacin (ENRO), Gentamycin (GEN)에 대한 내성 여부를 확인하였다. 이중 TC에 내성을 보이는 균주는 PCR법을 통해 tet 유전자의 분포를 조사하였다. 그 결과, 총 101개 균주 중에서 Aeromonas spp. 가 44개(43.5%)로 가장 우점하였고, 그 다음으로 Pseudomonas spp. 4개(4.0%), Vibrio spp. 5개(5.0%)가 확인되었다. 또한, 다중 내성을 보이는 균주(77.2%)가 단일 내성균(22.8%)보다 많음을 확인하였다. tet(A), tet(D), tet(E), tet(G), tet(M), tet(S)가 TC 내성 균주에서 검출되었으며 tet(A)가 가장 우점적으로 확인되었다. Aeromonas spp.는 분리된 균주 중에서 가장 많았으며, 경기도 내 양식현장에서의 다양한 항생제 내성 유전자의 특성에 대한 추가적인 연구가 필요하다.

KCI후보

7First report of Paranophrys marina (Protozoa, Ciliophora, Scuticociliatia) isolated from olive flounder Paralichthys olivaceus in Korea: morphological and phylogenetic analysis

저자 : Hyun-sil Kang , Ilson Whang , Jae-kwon Cho

발행기관 : 한국어병학회 간행물 : 한국어병학회지 34권 1호 발행 연도 : 2021 페이지 : pp. 47-53 (7 pages)

다운로드

(기관인증 필요)

초록보기

Scuticociliates are one of the serious parasitic threats faced by the marine aquaculturists worldwide. To date, Uronema nigricans, Philasterides dicentrarchi, Miamiensis avidus, Uronema marinum, and Pseudocohnilembus persalinus have been reported as the important culprit species causing scuticociliatosis in fish. The present paper reports the finding of an additional scuticociliate isolate from the gill of diseased olive flounder Paralichthys olivaceus in Korea. Based on the morphological characteristics, a scuticociliate in this study was identified as Paranophrys marina. Phylogenetic analysis placed P. marina as a sister lineage to three species of Pseudocohnilembus and Mesanophrys carcini within the order Philasterida.

KCI후보

8Infection status and microhabitat of polyopisthocotylean Monogenea found on the gills of goldlined seabream, Rhabdosargus sarba, from Musairah Island, Oman

저자 : Maryam Al Sawafi , Miyoung Cho , Adil Al Sulaimani , Gilha Yoon

발행기관 : 한국어병학회 간행물 : 한국어병학회지 34권 1호 발행 연도 : 2021 페이지 : pp. 55-61 (7 pages)

다운로드

(기관인증 필요)

초록보기

One of the major problems in fish production is that parasite-induced episodes of disease can result in significant impacts to the health of stock, mortalities and, economical losses. Seabreams are economically important fish in both the capture fishery and aquaculture sectors in Oman and represent potential species for aquaculture. The current study set out to investigate infection of polyopisthocotylean monogeneans on the gills of wild goldlined seabream, Rhabdosargus sarba, caught from Musairah region in Oman. The prevalence, mean intensity and abundance of polyopisthocotyleans on 145 fish was found to be 52.41%, 5.65 and 3.04, respectively. Three species of microcotylid parasite were isolated, one being Atrispinum acarne while the remaining two species require further study to identify them. There was no significant difference in the infection rate between host sex, however, parasite burdens were higher on larger sized fish (p<0.05) and were found to favour the first gill arch (p< 0.05). The study found that the sample of Rhabdosargus sarba landed in the waters around Musairah are heavily infected, with a predominantly immature population of microcotylids. Further work is required to better understand the potential risk each species poses to the health of its host before a decision is made on the suitability of new candidate fish species for commercial aquaculture.

KCI후보

9멍게 물렁증 근육변성의 병인에 관한 연구

저자 : 허민도 ( Min Do Huh ) , 이효은 ( Hyo Eun Lee ) , 이무근 ( Mu Kun Lee ) , 김보성 ( Bo Sung Kim )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 34권 1호 발행 연도 : 2021 페이지 : pp. 63-70 (8 pages)

다운로드

(기관인증 필요)

초록보기

As a part of research to elucidate the pathogenesis of so called Soft Tunic Syndrome(STS), that caused mass mortalities in the cultured sea squirts, Halocynthia roretzi, the epidemiological and pathological analysis were done to both clinically normal and diseased groups of the farms of Tongyoung and Geoje coastal areas in southeast sea from February to July, 2008. In the histological finding of the tunic, most of individuals showed tunic softness syndromes that included the disarrangement and destruction of tunic fiber with the simultaneous presence of flagellates-like cells, recently suspected as main agents of tunic softness syndromes. Simultaneously, the intensive degenerative changes of the skeletal muscle of diseased sea squirts were recognized. The changes were characterized with the hyalinization and condensation of muscle fibril and hemocytic infiltration in the muscle fibers. Those were thought to be a kind of typical Zenker's necrosis as in the skeletal muscle of higher vertebrates. Besides of the diseased sea squirts, Zenker's necrosis of skeletal muscles were seen in the normal ones. Epidemiological inquiry for diseased groups revealed that the higher incidences of tunic softness syndrome were recorded in the fast growing groups and in the sites presuming the organic pollution. And Higher malondialadehyde(MDA) and glutathione peroxidase(GPx) activity were detected in the groups showing STS. Those results suggested that Zenker's necrosis of body muscles was a kind of“nutritional myopathy" by oxidative stress. Conclusively, it was considered that Zenker's necrosis of body muscles gives an important clue for elucidating pathogenesis of STS of cultured squirts. And it seems that the necrosis were caused by the oxidative stress to body muscle during abnormal rapid growth of sea squirts.

KCI후보

10흰다리새우(Litopenaeus vannamei)에서 cephalexin의 잔류농도 연구를 위한 LC-MS/MS 분석법 연구

저자 : 양찬영 ( Chan Yeong Yang ) , 배준성 ( Jun Sung Bae ) , 이채원 ( Chae Won Lee ) , 정은하 ( Eun Ha Jeong ) , 이지훈 ( Ji-hoon Lee ) , 박수진 ( Su-jin Bak ) , 최상훈 ( Sang-hoon Choi ) , 박관하 ( Kwan Ha Park )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 34권 1호 발행 연도 : 2021 페이지 : pp. 71-80 (10 pages)

다운로드

(기관인증 필요)

초록보기

Cephalexin, a semi-synthetic cephalosporin antibiotic, has long been used in fish aquaculture in various countries under legal authorization. The drug is thus widely available for use in other aquatic species except fishes like the crustacean whiteleg shrimp. This study aims to develop a sensitive method for laboratory residue studies to adopt in withdrawal period determinations. Through repeated trials from the existing methods developed for other food animal tissues, it was possible to achieve a sensitive high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method. The results showed that at a concentration of 0.1 mg/kg, the recovery rate was 81.79%, and C.V. value was 8.2%, which meet the recovery rate and C.V. recommended by Codex guideline. After satisfactory validation of analytical procedures, applicability to the shrimp tissue was confirmed in experimentally cephalexin-treated whiteleg shrimp. As a result, most muscle samples were detected below the limit of quantification (0.05 mg/kg) after day 3, and most hepatopancreas samples were detected below the limit of quantification after day 14. In particular, the limit of quantification 0.05 ppm with the presently developed method suggests sufficient sensitive over the current legal maximum residue limit of 0.2 mg/kg set for fishes.

12
발행기관 최신논문
자료제공: 네이버학술정보
발행기관 최신논문
자료제공: 네이버학술정보

내가 찾은 최근 검색어

최근 열람 자료

맞춤 논문

보관함

내 보관함
공유한 보관함

1:1문의

닫기