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대한수의학회> Korean Journal of Veterinary Research(구 대한수의학회지)> 토끼에서 Pasteurella multocida 감염에 의한 뇌막뇌염과 폐렴 증례보고

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토끼에서 Pasteurella multocida 감염에 의한 뇌막뇌염과 폐렴 증례보고

Meningoencephalitis and pneumonia caused by Pasteurella multocida in rabbits

정지연 ( Jiyeon Jeong ) , 이경현 ( Kyunghyun Lee ) , 최은진 ( Eun-jin Choi ) , 김하영 ( Ha-young Kim ) , 손준형 ( Jun Hyung Sohn ) , 소병재 ( Byungjae So ) , 정지열 ( Ji-youl Jung )
  • : 대한수의학회
  • : Korean Journal of Veterinary Research(구 대한수의학회지) 58권1호
  • : 연속간행물
  • : 2018년 03월
  • : 61-64(4pages)
Korean Journal of Veterinary Research(구 대한수의학회지)

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Eight rabbits exhibited head tilt and subsequently died. At necropsy, three rabbits had crusty deposits in ears and four had reddish lungs. The main histopathological features were severe diffuse suppurative meningoencephalitis (75.0% of rabbits), fibrinopurulent pneumonia (37.5%), and otitis externa (37.5%). Pasteurella multocida (P. multocida) was isolated from brains, ears, and lungs. The capsular serogroups of the isolates were untypable. Based on histopathological features and bacterial analysis results, the rabbits were diagnosed as P. multocida infection. P. multocida infections might result in considerable economic loss in commercial rabbit production facilities in Korea.

UCI(KEPA)

I410-ECN-0102-2018-500-004256980

간행물정보

  • : 농학분야  > 농공학
  • : KCI등재
  • : SCOPUS
  • : 계간
  • : 2466-1384
  • : 2466-1392
  • : 학술지
  • : 연속간행물
  • : 1961-2018
  • : 3053


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1Artemisia capillaris Thunb. inhibits melanin synthesis activity via ERK-dependent MITF pathway in B16/F10 melanoma cells

저자 : Evelyn Saba , Mi Ju Oh , Yuan Yee Lee , Dongmi Kwak , Suk Kim , Man Hee Rhee

발행기관 : 대한수의학회 간행물 : Korean Journal of Veterinary Research(구 대한수의학회지) 58권 1호 발행 연도 : 2018 페이지 : pp. 1-7 (7 pages)

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Genus Artemisia occurs as a hardy plant and has a wide range of culinary and medicinal features. In this study, we aimed to describe the melanin inhibitory activity of one Artemisia species, i.e., Artemisia capillaris Thunb. Ethanol extracts of fermented Artemisia capillaris (Art.EtOH.FT) and non-fermented Artemisia capillaris (Art.EtOH.CT) were tested for their ability to inhibit tyrosinase activity and melanin pigmentation. Both extracts showed dose-dependent inhibition against α-melanocyte stimulating hormone-stimulated melanin formation and tyrosinase activity, without cytotoxicity. At 100 μg/mL, both extracts showed greater inhibition than kojic acid, the positive control. Protein expressions of microphthalmia-associated transcription factor (MITF), tyrosinase (TYR), tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2) at the transcriptional level were determined by using real-time and semi-quantitative polymerase chain reaction. To complete the mechanistic study, presences of upstream elements of MITF, the phosphorylated-extracellular signal-regulated kinase (p-ERK), and phosphorylated-mitogen-activated protein kinase kinase (p-MEK) were confirmed by using western blot analysis. Expressions of p-TYR, p-TRP-1 and p-TRP- 2, downstream factors for p-ERK and p-MITF, were translationally inhibited by both extracts. Art.EtOH.FT induced more potent effects than Art.EtOH.CT, especially signal transduction effects. In summary, Artemisia capillaris extracts appear to act as potent hypopigmentation agents.

KCI등재 SCOPUS

2Preliminary assessment of correlation between T-lymphocyte responses and control of porcine reproductive and respiratory syndrome virus (PRRSV) in piglets born after in-utero infection of a type 2 PRRSV

저자 : Sang-ho Cha , Carey Bandaranayaka-mudiyanselage , Chandima B. Bandaranayaka-mudiyanselage , Dharani Ajiththos , Kyoung-jin Yoon , Kathleen A. Gibson , Ji-eun Yu , In-soo Cho , Stephen S. Lee , Chungwon J. Chung

발행기관 : 대한수의학회 간행물 : Korean Journal of Veterinary Research(구 대한수의학회지) 58권 1호 발행 연도 : 2018 페이지 : pp. 9-16 (8 pages)

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A preliminary study into the protective mechanisms of adaptive immunity against porcine reproductive and respiratory syndrome virus (PRRSV) in piglets (n = 9) born to a gilt challenged intranasally with a type-2 PRRSV. Immune parameters (neutralizing antibodies, CD3+CD4+, CD3+CD8+, CD3+CD4+CD8+ T-lymphocytes, and PRRSVspecific interferon (IFN)-γ secreting T-lymphocytes) were compared with infection parameters (macro- and microscopic lung lesion, and PRRSV-infected porcine alveolar macrophages (CD172α +PRRSV-N+ PAM) as well as with plasma and lymphoid tissue viral loads. Percentages of three T-lymphocyte phenotypes in 14-days post-birth (dpb) peripheral blood mononuclear cell (PBMC) had significant negative correlations with percentages of CD172α +PRRSV-N+ PAM (p < 0.05) as well as with macroscopic lung lesion (p < 0.01). Plasma and tissue viral loads had significant (p < 0.05) negative correlations with CD3+CD4+CD8+ T-lymphocyte percentage in PBMC. Frequencies of CD3+CD8+ and CD3+CD4+ T-lymphocytes in 14-dpb PBMC had significant negative correlations with of lymph node (p = 0.04) and lung (p = 0.002) viral loads. IFN-γ-secreting T-lymphocytes frequency had a significant negative correlation with gross lung lesion severity (p = 0.002). However, neutralizing antibody titers had no significant negative correlation (p > 0.1) with infection parameters. The results indicate that T-lymphocytes contribute to controlling PRRSV replication in young piglets born after in-utero infection.

KCI등재 SCOPUS

3Knowledge, attitudes and practices toward antimicrobial usage: a cross-sectional study of layer and pig farm owners/managers in Chiang Mai, Lamphun, and Chonburi provinces, Thailand, May 2014 to February 2016

저자 : Aniroot Nuangmek , Suvichai Rojanasthien , Prapas Patchanee , Terdsak Yano , Panuwat Yamsakul , Suwit Chotinun , Pakpoom Tadee

발행기관 : 대한수의학회 간행물 : Korean Journal of Veterinary Research(구 대한수의학회지) 58권 1호 발행 연도 : 2018 페이지 : pp. 17-25 (9 pages)

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This study aimed at determining the current knowledge, attitude, and practices (KAP) of layer and pig farmers to antimicrobial usage and its consequences. Description of such KAP could provide insights useful for promoting the rational use of antimicrobials in livestock. From May 2014 to February 2016, a survey involving 251 respondents in Chiang Mai, Lamphun, and Chonburi provinces, Thailand, was conducted by using a validated questionnaire. More than half (51.0%) of the respondents incorrectly believed that antimicrobial drug efficacy could not be reduced by using sub-recommended dosages, 61.2% had misconceptions about non-therapeutic antimicrobial use, and 66.9% inaccurately felt that antimicrobials were also effective for virus and fungi. Over half (50.6-55.2%) did not see the need to follow instructions or advice of veterinarians. Moreover, only 10.4% regularly relied on responses to drugs sensitivity tests when evaluating the effectiveness of antimicrobials. Overall, assessment of KAP regarding antimicrobial usage indicated that the majority of respondents had low levels of knowledge of antimicrobials, neutral rather than positive attitudes, and employed poor practices in the use of antimicrobials. The results indicate improvements in KAP could be helpful in developing more effective interventions by farmers, reduce antibiotic usage, and slow the growth of antimicrobial resistance.

KCI등재 SCOPUS

4Clinical evaluation of a rapid diagnostic test kit for detection of canine coronavirus

저자 : Seung-jae Yoon , Kyoung-won Seo , Kun-ho Song

발행기관 : 대한수의학회 간행물 : Korean Journal of Veterinary Research(구 대한수의학회지) 58권 1호 발행 연도 : 2018 페이지 : pp. 27-31 (5 pages)

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Canine coronavirus is a single-stranded RNA virus that causes enteritis in dogs of any age. Coronaviral enteritis is seldom definitively diagnosed, since it is usually much less severe than many other types of enteritis and is self-limiting. Conventional diagnostics for the canine coronaviral enteritis such as polymerase chain reaction (PCR), virus isolation, and electron microscopic examination are inappropriate for small animal clinics due to the complicated experimental processes involved. Therefore, a commercially available lateral flow test kit based on chromatographic immunoassay techniques was tested to evaluate its performance as a first-line diagnostic test kit that could be used in clinics. The coronavirus antigen test kit detected canine coronavirus-infected dogs with 93.1% sensitivity and 97.5% specificity. The detection limit of the test kit was between 1.97 × 104/mL and 9.85 × 103/mL for samples with a 2- fold serial dilution from 1.25 × 106 TCID50 (TCID50, 50% tissue culture infectious dose). Additionally, the test kit had no cross-reactivity with canine parvovirus, distemper virus, or Escherichia coli. Overall, the commercially available test kit showed good diagnostic performance in a clinical setting, with results similar to those from PCR, confirming their potential for convenient and accurate use in small animal clinics.

KCI등재 SCOPUS

5Flow cytometric immunophenotyping of canine adipose-derived mesenchymal stem cells (ADMSCs) and feline ADMSCs using anti-human antibodies

저자 : Minho Ko , Kwon Young Lee , Sae Hoon Kim , Manho Kim , Jung Hoon Choi , Wooseok Im , Jin Young Chung

발행기관 : 대한수의학회 간행물 : Korean Journal of Veterinary Research(구 대한수의학회지) 58권 1호 발행 연도 : 2018 페이지 : pp. 33-37 (5 pages)

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Various trials have been conducted to develop therapies for serious untreatable diseases. Among these, those using stem cells have shown great promise, and adipose-derived mesenchymal stem cells (ADMSCs) are easier to obtain than other types of stem cells. Prior to clinical trials, characterization of ADMSCs with monoclonal antibodies should be performed. However, it is difficult to use species-specific antibodies for veterinarians. This study was conducted to confirm the panel of human antibodies applicable for use in immunophenotypic characterization of canine adipose-derived stem cells and feline ADMSCs extracted from subcutaneous adipose tissue collected during ovariohysterectomy. For flow cytometric immunophenotyping, the third passages of canine ADMSC and feline ADMSC and human CD31, CD34, CD42, CD44, CD62 and CD133 antibodies were used. Of these, CD133 reacted with canine cells (3.74%) and feline cells (1.34%). CD133 is known as a marker related with more primitive stem cell phenotype than other CD series. Because this human CD133 was not a species-specific antibody, accurate percentages of immunoreactivity were not confirmed. Nevertheless, the results of this study confirmed human CD133 as a meaningful marker in canine and feline ADMSCs.

KCI등재 SCOPUS

6Biological control of Flavobacterium psychrophilum infection in ayu (Plecoglossus altivelis altivelis) using a bacteriophage PFpW-3

저자 : Ji Hyung Kim , Se Chang Park

발행기관 : 대한수의학회 간행물 : Korean Journal of Veterinary Research(구 대한수의학회지) 58권 1호 발행 연도 : 2018 페이지 : pp. 39-43 (5 pages)

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The efficacy of using a bacteriophage (phage) to control Flavobacterium psychrophilum (F. psychrophilum) infection of ayu (Plecoglossus altivelis altivelis) was evaluated in this study. Intramuscular challenge failed to induce sufficient infection levels; therefore, a newly designed net-scratch challenge method was also used to induce bacterial infection. Administration of phage PFpW-3 in F. psychrophilum-infected ayu showed notable protective effects, increased survival rates and mean times to death. Additionally, the fate of inoculated bacteria and phage in ayu were investigated. Our results suggest that the phage PFpW-3 could be considered an alternative biocontrol agent against F. psychrophilum infections in ayu culture.

KCI등재 SCOPUS

7육용종계와 산란계에서 avian hepatitis E virus의 검출 및 특성 규명

저자 : 문현우 ( Hyun-woo Moon ) , 성환우 ( Haan Woo Sung ) , 권혁무 ( Hyuk Moo Kwon )

발행기관 : 대한수의학회 간행물 : Korean Journal of Veterinary Research(구 대한수의학회지) 58권 1호 발행 연도 : 2018 페이지 : pp. 45-49 (5 pages)

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The helicase genes and hypervariable regions (HVRs) of three avian hepatitis E viruses (HEVs) detected at three different farms were sequenced and characterized. Two isolates (DW-L and GI-B2) were classified as genotype 2 and one isolate (GR-B) was classified as genotype 1. A phylogenetic tree, based on the helicase gene and HVR nucleotide sequences, revealed the newly detected viruses and other avian HEVs were classified similarly. Unlike previously reported avian HEVs, the DW-L isolate detected in broiler breeders with characteristic lesions of avian HEV had no prolinerich motif in its HVR, suggesting that the proline-rich motif is non-essential for viral replication and infection.

KCI등재 SCOPUS

8rpoB 염기서열 분석을 이용한 응고효소 음성 포도알세균 분자 동정

저자 : 성원진 ( Won-jin Seong ) , 김단일 ( Danil Kim ) , 김은경 ( Eun-kyung Kim ) , 고대성 ( Dae-sung Ko ) , 노영혜 ( Younghye Ro ) , 김재홍 ( Jae-hong Kim ) , 권혁준 ( Hyuk-joon Kwon )

발행기관 : 대한수의학회 간행물 : Korean Journal of Veterinary Research(구 대한수의학회지) 58권 1호 발행 연도 : 2018 페이지 : pp. 51-55 (5 pages)

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Bovine mastitis (BM) has resulted in enormous economic loss in the dairy industry and coagulase-negative staphylococci (CNS) have caused subclinical BM. Although VITEK 2 GP ID card (VITEK 2) has been used for CNS identification, the probability of identification varies. The rpoB sequence typing (RSTing) method has been used for molecular diagnosis and epidemiology of bacterial infections. In this study, we undertook RSTing of CNS and compared the results with those of VITEK2 and 16S rRNA gene sequencing. As compared VITEK2, the molecular-based methods were more reliable for species identification; moreover, RSTing provided more molecular epidemiological information than that from 16S rRNA gene sequencing.

KCI등재 SCOPUS

9Periarticular histiocytic sarcoma of a thoracic limb in a Rottweiler

저자 : Hyeok-soo Shin , Ye-in Oh , Byung-jae Kang

발행기관 : 대한수의학회 간행물 : Korean Journal of Veterinary Research(구 대한수의학회지) 58권 1호 발행 연도 : 2018 페이지 : pp. 57-60 (4 pages)

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An 8-year-old, castrated, male Rottweiler was referred for evaluation of chronic right thoracic limb lameness and a progressively growing mass surrounding the right elbow joint. On admission, the dog's general health was good, without abnormalities detected on physical examination. The dog was diagnosed with periarticular histiocytic sarcoma. Although draining lymph nodes and lung metastases were suspected, palliative amputation was performed. Localized histiocytic sarcomas, with destructive lesions involving multiple bones of a joint and periarticular soft-tissue masses, are uncommon in dogs. This case report presents clinical findings, imaging characteristics, and histopathologic and immunohistochemical features of a periarticular joint histiocytic sarcoma.

KCI등재 SCOPUS

10토끼에서 Pasteurella multocida 감염에 의한 뇌막뇌염과 폐렴 증례보고

저자 : 정지연 ( Jiyeon Jeong ) , 이경현 ( Kyunghyun Lee ) , 최은진 ( Eun-jin Choi ) , 김하영 ( Ha-young Kim ) , 손준형 ( Jun Hyung Sohn ) , 소병재 ( Byungjae So ) , 정지열 ( Ji-youl Jung )

발행기관 : 대한수의학회 간행물 : Korean Journal of Veterinary Research(구 대한수의학회지) 58권 1호 발행 연도 : 2018 페이지 : pp. 61-64 (4 pages)

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Eight rabbits exhibited head tilt and subsequently died. At necropsy, three rabbits had crusty deposits in ears and four had reddish lungs. The main histopathological features were severe diffuse suppurative meningoencephalitis (75.0% of rabbits), fibrinopurulent pneumonia (37.5%), and otitis externa (37.5%). Pasteurella multocida (P. multocida) was isolated from brains, ears, and lungs. The capsular serogroups of the isolates were untypable. Based on histopathological features and bacterial analysis results, the rabbits were diagnosed as P. multocida infection. P. multocida infections might result in considerable economic loss in commercial rabbit production facilities in Korea.

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1Artemisia capillaris Thunb. inhibits melanin synthesis activity via ERK-dependent MITF pathway in B16/F10 melanoma cells

저자 : Evelyn Saba , Mi Ju Oh , Yuan Yee Lee , Dongmi Kwak , Suk Kim , Man Hee Rhee

발행기관 : 대한수의학회 간행물 : Korean Journal of Veterinary Research(구 대한수의학회지) 58권 1호 발행 연도 : 2018 페이지 : pp. 1-7 (7 pages)

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Genus Artemisia occurs as a hardy plant and has a wide range of culinary and medicinal features. In this study, we aimed to describe the melanin inhibitory activity of one Artemisia species, i.e., Artemisia capillaris Thunb. Ethanol extracts of fermented Artemisia capillaris (Art.EtOH.FT) and non-fermented Artemisia capillaris (Art.EtOH.CT) were tested for their ability to inhibit tyrosinase activity and melanin pigmentation. Both extracts showed dose-dependent inhibition against α-melanocyte stimulating hormone-stimulated melanin formation and tyrosinase activity, without cytotoxicity. At 100 μg/mL, both extracts showed greater inhibition than kojic acid, the positive control. Protein expressions of microphthalmia-associated transcription factor (MITF), tyrosinase (TYR), tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2) at the transcriptional level were determined by using real-time and semi-quantitative polymerase chain reaction. To complete the mechanistic study, presences of upstream elements of MITF, the phosphorylated-extracellular signal-regulated kinase (p-ERK), and phosphorylated-mitogen-activated protein kinase kinase (p-MEK) were confirmed by using western blot analysis. Expressions of p-TYR, p-TRP-1 and p-TRP- 2, downstream factors for p-ERK and p-MITF, were translationally inhibited by both extracts. Art.EtOH.FT induced more potent effects than Art.EtOH.CT, especially signal transduction effects. In summary, Artemisia capillaris extracts appear to act as potent hypopigmentation agents.

KCI등재SCOUPUS

2Preliminary assessment of correlation between T-lymphocyte responses and control of porcine reproductive and respiratory syndrome virus (PRRSV) in piglets born after in-utero infection of a type 2 PRRSV

저자 : Sang-ho Cha , Carey Bandaranayaka-mudiyanselage , Chandima B. Bandaranayaka-mudiyanselage , Dharani Ajiththos , Kyoung-jin Yoon , Kathleen A. Gibson , Ji-eun Yu , In-soo Cho , Stephen S. Lee , Chungwon J. Chung

발행기관 : 대한수의학회 간행물 : Korean Journal of Veterinary Research(구 대한수의학회지) 58권 1호 발행 연도 : 2018 페이지 : pp. 9-16 (8 pages)

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A preliminary study into the protective mechanisms of adaptive immunity against porcine reproductive and respiratory syndrome virus (PRRSV) in piglets (n = 9) born to a gilt challenged intranasally with a type-2 PRRSV. Immune parameters (neutralizing antibodies, CD3+CD4+, CD3+CD8+, CD3+CD4+CD8+ T-lymphocytes, and PRRSVspecific interferon (IFN)-γ secreting T-lymphocytes) were compared with infection parameters (macro- and microscopic lung lesion, and PRRSV-infected porcine alveolar macrophages (CD172α +PRRSV-N+ PAM) as well as with plasma and lymphoid tissue viral loads. Percentages of three T-lymphocyte phenotypes in 14-days post-birth (dpb) peripheral blood mononuclear cell (PBMC) had significant negative correlations with percentages of CD172α +PRRSV-N+ PAM (p < 0.05) as well as with macroscopic lung lesion (p < 0.01). Plasma and tissue viral loads had significant (p < 0.05) negative correlations with CD3+CD4+CD8+ T-lymphocyte percentage in PBMC. Frequencies of CD3+CD8+ and CD3+CD4+ T-lymphocytes in 14-dpb PBMC had significant negative correlations with of lymph node (p = 0.04) and lung (p = 0.002) viral loads. IFN-γ-secreting T-lymphocytes frequency had a significant negative correlation with gross lung lesion severity (p = 0.002). However, neutralizing antibody titers had no significant negative correlation (p > 0.1) with infection parameters. The results indicate that T-lymphocytes contribute to controlling PRRSV replication in young piglets born after in-utero infection.

KCI등재SCOUPUS

3Knowledge, attitudes and practices toward antimicrobial usage: a cross-sectional study of layer and pig farm owners/managers in Chiang Mai, Lamphun, and Chonburi provinces, Thailand, May 2014 to February 2016

저자 : Aniroot Nuangmek , Suvichai Rojanasthien , Prapas Patchanee , Terdsak Yano , Panuwat Yamsakul , Suwit Chotinun , Pakpoom Tadee

발행기관 : 대한수의학회 간행물 : Korean Journal of Veterinary Research(구 대한수의학회지) 58권 1호 발행 연도 : 2018 페이지 : pp. 17-25 (9 pages)

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This study aimed at determining the current knowledge, attitude, and practices (KAP) of layer and pig farmers to antimicrobial usage and its consequences. Description of such KAP could provide insights useful for promoting the rational use of antimicrobials in livestock. From May 2014 to February 2016, a survey involving 251 respondents in Chiang Mai, Lamphun, and Chonburi provinces, Thailand, was conducted by using a validated questionnaire. More than half (51.0%) of the respondents incorrectly believed that antimicrobial drug efficacy could not be reduced by using sub-recommended dosages, 61.2% had misconceptions about non-therapeutic antimicrobial use, and 66.9% inaccurately felt that antimicrobials were also effective for virus and fungi. Over half (50.6-55.2%) did not see the need to follow instructions or advice of veterinarians. Moreover, only 10.4% regularly relied on responses to drugs sensitivity tests when evaluating the effectiveness of antimicrobials. Overall, assessment of KAP regarding antimicrobial usage indicated that the majority of respondents had low levels of knowledge of antimicrobials, neutral rather than positive attitudes, and employed poor practices in the use of antimicrobials. The results indicate improvements in KAP could be helpful in developing more effective interventions by farmers, reduce antibiotic usage, and slow the growth of antimicrobial resistance.

KCI등재SCOUPUS

4Clinical evaluation of a rapid diagnostic test kit for detection of canine coronavirus

저자 : Seung-jae Yoon , Kyoung-won Seo , Kun-ho Song

발행기관 : 대한수의학회 간행물 : Korean Journal of Veterinary Research(구 대한수의학회지) 58권 1호 발행 연도 : 2018 페이지 : pp. 27-31 (5 pages)

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Canine coronavirus is a single-stranded RNA virus that causes enteritis in dogs of any age. Coronaviral enteritis is seldom definitively diagnosed, since it is usually much less severe than many other types of enteritis and is self-limiting. Conventional diagnostics for the canine coronaviral enteritis such as polymerase chain reaction (PCR), virus isolation, and electron microscopic examination are inappropriate for small animal clinics due to the complicated experimental processes involved. Therefore, a commercially available lateral flow test kit based on chromatographic immunoassay techniques was tested to evaluate its performance as a first-line diagnostic test kit that could be used in clinics. The coronavirus antigen test kit detected canine coronavirus-infected dogs with 93.1% sensitivity and 97.5% specificity. The detection limit of the test kit was between 1.97 × 104/mL and 9.85 × 103/mL for samples with a 2- fold serial dilution from 1.25 × 106 TCID50 (TCID50, 50% tissue culture infectious dose). Additionally, the test kit had no cross-reactivity with canine parvovirus, distemper virus, or Escherichia coli. Overall, the commercially available test kit showed good diagnostic performance in a clinical setting, with results similar to those from PCR, confirming their potential for convenient and accurate use in small animal clinics.

KCI등재SCOUPUS

5Flow cytometric immunophenotyping of canine adipose-derived mesenchymal stem cells (ADMSCs) and feline ADMSCs using anti-human antibodies

저자 : Minho Ko , Kwon Young Lee , Sae Hoon Kim , Manho Kim , Jung Hoon Choi , Wooseok Im , Jin Young Chung

발행기관 : 대한수의학회 간행물 : Korean Journal of Veterinary Research(구 대한수의학회지) 58권 1호 발행 연도 : 2018 페이지 : pp. 33-37 (5 pages)

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Various trials have been conducted to develop therapies for serious untreatable diseases. Among these, those using stem cells have shown great promise, and adipose-derived mesenchymal stem cells (ADMSCs) are easier to obtain than other types of stem cells. Prior to clinical trials, characterization of ADMSCs with monoclonal antibodies should be performed. However, it is difficult to use species-specific antibodies for veterinarians. This study was conducted to confirm the panel of human antibodies applicable for use in immunophenotypic characterization of canine adipose-derived stem cells and feline ADMSCs extracted from subcutaneous adipose tissue collected during ovariohysterectomy. For flow cytometric immunophenotyping, the third passages of canine ADMSC and feline ADMSC and human CD31, CD34, CD42, CD44, CD62 and CD133 antibodies were used. Of these, CD133 reacted with canine cells (3.74%) and feline cells (1.34%). CD133 is known as a marker related with more primitive stem cell phenotype than other CD series. Because this human CD133 was not a species-specific antibody, accurate percentages of immunoreactivity were not confirmed. Nevertheless, the results of this study confirmed human CD133 as a meaningful marker in canine and feline ADMSCs.

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6Biological control of Flavobacterium psychrophilum infection in ayu (Plecoglossus altivelis altivelis) using a bacteriophage PFpW-3

저자 : Ji Hyung Kim , Se Chang Park

발행기관 : 대한수의학회 간행물 : Korean Journal of Veterinary Research(구 대한수의학회지) 58권 1호 발행 연도 : 2018 페이지 : pp. 39-43 (5 pages)

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The efficacy of using a bacteriophage (phage) to control Flavobacterium psychrophilum (F. psychrophilum) infection of ayu (Plecoglossus altivelis altivelis) was evaluated in this study. Intramuscular challenge failed to induce sufficient infection levels; therefore, a newly designed net-scratch challenge method was also used to induce bacterial infection. Administration of phage PFpW-3 in F. psychrophilum-infected ayu showed notable protective effects, increased survival rates and mean times to death. Additionally, the fate of inoculated bacteria and phage in ayu were investigated. Our results suggest that the phage PFpW-3 could be considered an alternative biocontrol agent against F. psychrophilum infections in ayu culture.

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7육용종계와 산란계에서 avian hepatitis E virus의 검출 및 특성 규명

저자 : 문현우 ( Hyun-woo Moon ) , 성환우 ( Haan Woo Sung ) , 권혁무 ( Hyuk Moo Kwon )

발행기관 : 대한수의학회 간행물 : Korean Journal of Veterinary Research(구 대한수의학회지) 58권 1호 발행 연도 : 2018 페이지 : pp. 45-49 (5 pages)

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The helicase genes and hypervariable regions (HVRs) of three avian hepatitis E viruses (HEVs) detected at three different farms were sequenced and characterized. Two isolates (DW-L and GI-B2) were classified as genotype 2 and one isolate (GR-B) was classified as genotype 1. A phylogenetic tree, based on the helicase gene and HVR nucleotide sequences, revealed the newly detected viruses and other avian HEVs were classified similarly. Unlike previously reported avian HEVs, the DW-L isolate detected in broiler breeders with characteristic lesions of avian HEV had no prolinerich motif in its HVR, suggesting that the proline-rich motif is non-essential for viral replication and infection.

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8rpoB 염기서열 분석을 이용한 응고효소 음성 포도알세균 분자 동정

저자 : 성원진 ( Won-jin Seong ) , 김단일 ( Danil Kim ) , 김은경 ( Eun-kyung Kim ) , 고대성 ( Dae-sung Ko ) , 노영혜 ( Younghye Ro ) , 김재홍 ( Jae-hong Kim ) , 권혁준 ( Hyuk-joon Kwon )

발행기관 : 대한수의학회 간행물 : Korean Journal of Veterinary Research(구 대한수의학회지) 58권 1호 발행 연도 : 2018 페이지 : pp. 51-55 (5 pages)

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Bovine mastitis (BM) has resulted in enormous economic loss in the dairy industry and coagulase-negative staphylococci (CNS) have caused subclinical BM. Although VITEK 2 GP ID card (VITEK 2) has been used for CNS identification, the probability of identification varies. The rpoB sequence typing (RSTing) method has been used for molecular diagnosis and epidemiology of bacterial infections. In this study, we undertook RSTing of CNS and compared the results with those of VITEK2 and 16S rRNA gene sequencing. As compared VITEK2, the molecular-based methods were more reliable for species identification; moreover, RSTing provided more molecular epidemiological information than that from 16S rRNA gene sequencing.

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9Periarticular histiocytic sarcoma of a thoracic limb in a Rottweiler

저자 : Hyeok-soo Shin , Ye-in Oh , Byung-jae Kang

발행기관 : 대한수의학회 간행물 : Korean Journal of Veterinary Research(구 대한수의학회지) 58권 1호 발행 연도 : 2018 페이지 : pp. 57-60 (4 pages)

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An 8-year-old, castrated, male Rottweiler was referred for evaluation of chronic right thoracic limb lameness and a progressively growing mass surrounding the right elbow joint. On admission, the dog's general health was good, without abnormalities detected on physical examination. The dog was diagnosed with periarticular histiocytic sarcoma. Although draining lymph nodes and lung metastases were suspected, palliative amputation was performed. Localized histiocytic sarcomas, with destructive lesions involving multiple bones of a joint and periarticular soft-tissue masses, are uncommon in dogs. This case report presents clinical findings, imaging characteristics, and histopathologic and immunohistochemical features of a periarticular joint histiocytic sarcoma.

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10토끼에서 Pasteurella multocida 감염에 의한 뇌막뇌염과 폐렴 증례보고

저자 : 정지연 ( Jiyeon Jeong ) , 이경현 ( Kyunghyun Lee ) , 최은진 ( Eun-jin Choi ) , 김하영 ( Ha-young Kim ) , 손준형 ( Jun Hyung Sohn ) , 소병재 ( Byungjae So ) , 정지열 ( Ji-youl Jung )

발행기관 : 대한수의학회 간행물 : Korean Journal of Veterinary Research(구 대한수의학회지) 58권 1호 발행 연도 : 2018 페이지 : pp. 61-64 (4 pages)

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Eight rabbits exhibited head tilt and subsequently died. At necropsy, three rabbits had crusty deposits in ears and four had reddish lungs. The main histopathological features were severe diffuse suppurative meningoencephalitis (75.0% of rabbits), fibrinopurulent pneumonia (37.5%), and otitis externa (37.5%). Pasteurella multocida (P. multocida) was isolated from brains, ears, and lungs. The capsular serogroups of the isolates were untypable. Based on histopathological features and bacterial analysis results, the rabbits were diagnosed as P. multocida infection. P. multocida infections might result in considerable economic loss in commercial rabbit production facilities in Korea.

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