Objectives: The purpose of this study is the research of the 2D:4D ratio and its correlation with the Sasang constitution and the MBTI preference types. Methods: A measurement of the 2D:4D ratios of 101 South Korean medical students was performed, the QSCC-II and PSSC-2004 were used to diagnose the Sasang constitution, and MBTI testing was performed to classify the preference types. Results: In the relationship between the Sasang constitution and the 2D:4D ratios, a statistically significant difference is absent in the 2D:4D ratios of the group comprising both sexes in the order of Taeumin, Soyangin, and Soeumin. A statistically significant difference is also lacking in the 2D:4D ratios of the male-only group in the order of Taeumin, Soyangin, and Soeumin. The 2D:4D ratios are higher in the female group only, but again a statistically significant difference is absent. In the 2D:4D ratios according to the MBTI preference type, the 2D:4D ratios of the I, S, F, and P types are higher in the unisex group, but a significant difference is not evident. The 2D:4D ratios of the E, N, F, and P types are higher in the male-only group, but again a significant difference is not evident. In the female-only group, the mean of the 2D:4D ratios of the I, S, F, and P types is higher, but a significant difference is not identifiable. Conclusions: The Sasang constitution and the MBTI preference types did not show statistically significant differences regarding the 2D:4D ratios of the male, female, and unisex groups.