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한국화학공학회> 화학공학의 이론과 응용> A-13 : Polymer Micro- and Nanospheres as Optical Glucose Biosensors

A-13 : Polymer Micro- and Nanospheres as Optical Glucose Biosensors

김범상 , Michael V. Pishko
  • : 한국화학공학회
  • : 화학공학의 이론과 응용 10권2호
  • : 연속간행물
  • : 2007년 10월
  • : 1130-1130(1pages)
화학공학의 이론과 응용

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Diabetes mellitus is a disease that inflicts an estimate 100 million people worldwide. Current commercial methods of blood glucose measurement require a blood sample, which is uncomfortable and opens the body to infection. Thus, a noninvasive monitoring approach could lead to better compliance and reduce the risk of infection. Glucose detection based upon fluorescence is one of the promising techniques because of its high sensitivity without interference from other constituents frequently found in blood plasma. Here, we describe the fabrication and characterization of poly(ethylene glycol) (PEG) hydrogel spheres containing chemically immobilized fluorophore-labeled Con A and dextran for potential use as optical glucose sensors. Fluorophore-labeled Con A and dextran were encapsulated in the PEG hydrogel spheres by reverse emulsion photopolymerization, yielding spheres from 500 nm to 20 μm. After sphere fabrication, the presence and leaching of Con A and dextran in the spheres was investigated. The fluorescence emission response of the PEG hydrogel spheres was also determined.

UCI(KEPA)

I410-ECN-0102-2015-500-001943111

간행물정보

  • : 공학분야  > 화학공학
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  • :
  • : 기타
  • : 1225-9004
  • :
  • : 학술지
  • : 연속간행물
  • : 1995-2007
  • : 3034


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1A-12 : Pilot scale polymerization using supercritical carbon dioxide as a reaction medium

저자 : 배원 , 신보현 , 권소영 , 김화용

발행기관 : 한국화학공학회 간행물 : 화학공학의 이론과 응용 10권 2호 발행 연도 : 2007 페이지 : pp. 1129-1129 (1 pages)

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Recently, the supercritical fluid technology is being widely used in many fields of polymer industries, including its use as a polymerization medium. CO2, especially, has been under a spotlight and studied as an alternative polymerization medium, since its critical conditions are relatively mild, and it is also nontoxic, inflammable, and inexpensive. In this research, Pilot scale (5 L for solution polymerization and 10 L for dispersion polymerization) reactors were designed and constructed for supercritical polymerization. Using these reactors, polymerizations were performed to reproduce the lab results. By optimizing the conditions of pilot scale reactors, discrete spherical polymeric particles could be obtained. In the case of PMMA, discrete spherical particle having the number average particle size of 5.72 ㎛, PSD of 1.06, and average molecular weight of 110,000 g/mol was successfully synthesized.

2A-13 : Polymer Micro- and Nanospheres as Optical Glucose Biosensors

저자 : 김범상 , Michael V. Pishko

발행기관 : 한국화학공학회 간행물 : 화학공학의 이론과 응용 10권 2호 발행 연도 : 2007 페이지 : pp. 1130-1130 (1 pages)

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Diabetes mellitus is a disease that inflicts an estimate 100 million people worldwide. Current commercial methods of blood glucose measurement require a blood sample, which is uncomfortable and opens the body to infection. Thus, a noninvasive monitoring approach could lead to better compliance and reduce the risk of infection. Glucose detection based upon fluorescence is one of the promising techniques because of its high sensitivity without interference from other constituents frequently found in blood plasma. Here, we describe the fabrication and characterization of poly(ethylene glycol) (PEG) hydrogel spheres containing chemically immobilized fluorophore-labeled Con A and dextran for potential use as optical glucose sensors. Fluorophore-labeled Con A and dextran were encapsulated in the PEG hydrogel spheres by reverse emulsion photopolymerization, yielding spheres from 500 nm to 20 μm. After sphere fabrication, the presence and leaching of Con A and dextran in the spheres was investigated. The fluorescence emission response of the PEG hydrogel spheres was also determined.

3A-14 : Preparation and charaterization of chitosan-based superporous semi-IPN hydrogels

저자 : 박효진 , 김덕준

발행기관 : 한국화학공학회 간행물 : 화학공학의 이론과 응용 10권 2호 발행 연도 : 2007 페이지 : pp. 1131-1131 (1 pages)

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The purpose of this study was to develop chitosan-based porous semi-interpenetrating polymer network(semi-IPN) for gastric retention device. Chitosan and glycol chitosan hydrogels with numerous pores were prepared, and their swelling behaviors in acidic solution were studied. And, to improve mechanical strength of hydrogels, PEO and PVA were used in IPN synthesis with glycol chitosan. Swelling studies were performed in different pH solution at 37°C. Morphology and pore size of hydrogels were confirmed by scanning electron microscopy(SEM). Mechanical strengths were determined by UTM.

4A-23 : Interplay between Cubic and Hexagonal Phases in Block Copolymer Solutions

저자 : 박문정 , 차국헌

발행기관 : 한국화학공학회 간행물 : 화학공학의 이론과 응용 10권 2호 발행 연도 : 2007 페이지 : pp. 1132-1132 (1 pages)

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The phase behavior of a styrene-isoprene diblock copolymer in a styrene-selective solvent was investigated by in-situ SAXS on isotropic and shear-oriented solutions, and by rheology and birefringence. A remarkable new feature in this phase diagram is the coexistence of both bcc and hcp sphere phases, in a region between close-packed spheres (cps) and hexagonally-packed cylinders (hex). By focusing on the transitions among these various ordered phases during heating and cooling cycles, we observed a strong hysteresis: supercooled cylinders persisted upon cooling. The stability of these supercooled cylinders is quite dependent on concentration and for high concentrations, the supercooled cylinders do not revert to spheres even after quiescent annealing for one month. The spontaneous formation of spheres due to the dissociation of cylinders is kinetically hindered in this case, and the system is apparently not amenable to any pre-transitional fluctuations of cylinders prior to the transition. This contrasts with the case of cylinders transforming to spheres on heating in the melt. Application of large amplitude shear to the supercooled cylinders is effective in restoring the equilibrium sphere phases.

5A-31 : pH-sensitive block copolymer micelle

저자 : 심우선 , 이두성

발행기관 : 한국화학공학회 간행물 : 화학공학의 이론과 응용 10권 2호 발행 연도 : 2007 페이지 : pp. 1133-1133 (1 pages)

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MPEG-PDLLA & MPEG-PCLA block copolymers were synthesized by a ring-opening copolymerization of D,L- lactide and ε-caprolactone with MPEG (monomethoxy poly (ethylene glycol)) as an initiator, respectively. The sulfamethazine was modified with succinic anhydride to conjugate the block copolymer with pH-sensitive moiety (sulfamethazine). Also, the oligo-sulfamethazine(OSM) was obtained by the radical polymerization with transfer agent. The conjugation of the block copolymer and modified sulfamethazine (or oligo-sulfamethazine) was done by the DCC coupling reaction. The micelles of the synthesized pH-sensitive biodegradable block copolymers were characterized by the fluorescence method in the various buffer solutions. Also, the micelle size was certificated by DLS. The critical micelle concentration of pH-sensitive block copolymers decreases with increasing the hydrophobic block length, and increases with increasing the pH of buffer solutions. Also, the pH-sensitive block copolymers show the micelle integration-disintegration due to the pH change at the constant concentration. This pH-sensitive block copolymer makes the drug targeting available, so it is expected to be applied as a superior drug carrier.

6A-33 : Preparation of microcellular polyolefin rubber(Engage)/clay nanocomposite foam in supercritical CO2

저자 : 이동석 , 배성렬 , 장영욱

발행기관 : 한국화학공학회 간행물 : 화학공학의 이론과 응용 10권 2호 발행 연도 : 2007 페이지 : pp. 1134-1134 (1 pages)

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In this study, we successfully generated polyolefin rubber(Engage) microcellular foam by using supercritical carbon dioxide as a physical blowing agent. The Engage/clay nanocomspotie were prepared by blending a organoclay(Closite 20A) with glycidyl methacrylate grafted Engage(Engage 8003) in a Haake internal mixer. From XRD analysis, it was found to form an intercalated nanocomposite. Foaming was performed with the neat Engage and its nanocomposite using supercritical carbon dioxide at 20MPa at 80℃. SEM analysis showed that microcellular foam with cell size in the range of 5 . 20μm were produced.Obviously, the nanocomposite exhibited smaller cell size and large cell density in comparison to neat Engage suggesting that the dispersed silicate particles act as nucleating sites for cell formation.

7A-42 : Micropatterning for Olfactory Bulb and Hippocampus Neuronal Network Structures

저자 : 안동준 , 최혜선

발행기관 : 한국화학공학회 간행물 : 화학공학의 이론과 응용 10권 2호 발행 연도 : 2007 페이지 : pp. 1135-1135 (1 pages)

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The control of neuronal cell adhesion and growth in artificially defined networks in vitro was developed for the study of neuronal signals propagation. Appropriate analysis is still greatly hindered by inherent morphological complexity of cultured neural network without any control in location of and/or synaptic connection between neurons. In order to build neural networks on a substrate, it is required to modify the substrate surface suitable for patterning neurons. Soft contact printing with a polydimethyl-siloxane (PDMS) stamp, which was fabricated by photolithography and molding, was used for controlling selective neuronal cell adhesion and growth. The experimental results indicate that the PDL(cytopilic) pattern stamping on PEG (cytophobic) background is effective in growing the hippocampal neuron cells and olfactory cells selectively along the PDL pattern. To optimize neuron growth and pattern forming neural networks, we counted cell body on node and neurits extended along track. And we traced how the neurons found PDL(cytophilic) tracks using dendrites to locate themselves on tracks.

8구두발표 : 고분자 4 ; A-44 : 광기능 폴리다이아세틸렌 초분자의 Chemical-Chip 응용

저자 : 양두호 , 최현 , 김은진 , 안동준

발행기관 : 한국화학공학회 간행물 : 화학공학의 이론과 응용 10권 2호 발행 연도 : 2007 페이지 : pp. 1136-1136 (1 pages)

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생체막을 모방한 폴리다이아세틸렌(polydiacetylene) 초분자는 온도, 기계적자극, pH 그리고 solvent 등의 외부자극에 의하여 청색(640nm)에서 적색(540nm)으로 색전이된다. 그리고 이러한 특성을 이용하여 cholera toxin, influenza virus, E-coli. 등을 검출할 수 있는 센서에 응용하고자 하는 연구가 진행되어 왔다. 본 연구에서는 DNA 칩이나 단백질 칩과 같이 micro array 형태로 폴리다이아세틸렌 초분자를 NH2로 처리한 유리 기판에 표면 패턴화한다. 표면 패턴화는 NHS와 NH2 사이에 amidization으로 유리 기판에 고정화한다. 패턴화된 유리 기판에 cyclodextrin(CD), polyallylamine (PAA), polyvinylalcohol(PVA), Glucose, vapor phase인 NH3과 HCl을 반응시켰다. CD, PAA, PVA, NH3, HCl는 초분자와 직접, Glucose는 Enzyme을 초분자에 고정화한 후 반응하였다. 그 결과 특정 초분자만 반응하여 색전이 되는 것을 fluorescence로 확인하였다. 그리고 초분자 표면에 ligands를 고정화하여 다양한 물질을 검출 가능할 것이다. 이는 유리 기판상에 센서의 기능을 가지고 있는 다양한 폴리다이아세틸렌 개발에 적용될것이다.

9P-283 : The effect of draw ratio and temperature on the drawing PET sheet

저자 : 문옥길 , 나재식

발행기관 : 한국화학공학회 간행물 : 화학공학의 이론과 응용 10권 2호 발행 연도 : 2007 페이지 : pp. 1137-1137 (1 pages)

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The effect of the double-stage uniaxial drawing process on the mechanical properties of PET sheet was investigated under different drawing conditions. At the double-stage drawing process, the PET sheet samples were stretched at different draw ratio and temperatures. The tensile strengths of each sample were measured on a universial test machine. The tensile strength of PET sheet was increased exponentially as the draw ratio was higher. It had a tendency to be improved by low draw temperature in the low draw ratio range. It showed that at double-stage drawing process, the tensile strength of PET sheet was strongly developed by low temperature and high draw ratio, the double-stage drawing process could be evolved more highly tensile strength of PET sheet than the single-stage drawing process.

10P-284 : Study of the vibration of friction wave on the drawn MoS2-Nylon 6 sheet

저자 : 문옥길 , 나재식

발행기관 : 한국화학공학회 간행물 : 화학공학의 이론과 응용 10권 2호 발행 연도 : 2007 페이지 : pp. 1138-1138 (1 pages)

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Vibration of friction wave of MoS2-filled nylon 6 sheet under high drawing were studied. Drawing MoS2-filled nylon 6 sheet was made by single-stage uniaxial drawing process. Waves of the friction coefficient were measured on a block-on-ring tester where sliding ocurred at a speed of 0.13m/s under 30lb. The curves of friction coefficient of drawing MoS2-Nylon have a similar tendency with non-drawing MoS2-Nylon and pure nylon. Wave vibration relate to the surface condition and hardness. Because that the rough surface of fretting damage behavior was displayed in drawing MoS2-Nylon sheet. This means that the surface of drawing MoS2-Nylon is more rough and hard than non-drawing MoS2-Nylon but tensile strength of drawing MoS2-Nylon is still better.

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1A-12 : Pilot scale polymerization using supercritical carbon dioxide as a reaction medium

저자 : 배원 , 신보현 , 권소영 , 김화용

발행기관 : 한국화학공학회 간행물 : 화학공학의 이론과 응용 10권 2호 발행 연도 : 2007 페이지 : pp. 1129-1129 (1 pages)

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Recently, the supercritical fluid technology is being widely used in many fields of polymer industries, including its use as a polymerization medium. CO2, especially, has been under a spotlight and studied as an alternative polymerization medium, since its critical conditions are relatively mild, and it is also nontoxic, inflammable, and inexpensive. In this research, Pilot scale (5 L for solution polymerization and 10 L for dispersion polymerization) reactors were designed and constructed for supercritical polymerization. Using these reactors, polymerizations were performed to reproduce the lab results. By optimizing the conditions of pilot scale reactors, discrete spherical polymeric particles could be obtained. In the case of PMMA, discrete spherical particle having the number average particle size of 5.72 ㎛, PSD of 1.06, and average molecular weight of 110,000 g/mol was successfully synthesized.

2A-13 : Polymer Micro- and Nanospheres as Optical Glucose Biosensors

저자 : 김범상 , Michael V. Pishko

발행기관 : 한국화학공학회 간행물 : 화학공학의 이론과 응용 10권 2호 발행 연도 : 2007 페이지 : pp. 1130-1130 (1 pages)

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Diabetes mellitus is a disease that inflicts an estimate 100 million people worldwide. Current commercial methods of blood glucose measurement require a blood sample, which is uncomfortable and opens the body to infection. Thus, a noninvasive monitoring approach could lead to better compliance and reduce the risk of infection. Glucose detection based upon fluorescence is one of the promising techniques because of its high sensitivity without interference from other constituents frequently found in blood plasma. Here, we describe the fabrication and characterization of poly(ethylene glycol) (PEG) hydrogel spheres containing chemically immobilized fluorophore-labeled Con A and dextran for potential use as optical glucose sensors. Fluorophore-labeled Con A and dextran were encapsulated in the PEG hydrogel spheres by reverse emulsion photopolymerization, yielding spheres from 500 nm to 20 μm. After sphere fabrication, the presence and leaching of Con A and dextran in the spheres was investigated. The fluorescence emission response of the PEG hydrogel spheres was also determined.

3A-14 : Preparation and charaterization of chitosan-based superporous semi-IPN hydrogels

저자 : 박효진 , 김덕준

발행기관 : 한국화학공학회 간행물 : 화학공학의 이론과 응용 10권 2호 발행 연도 : 2007 페이지 : pp. 1131-1131 (1 pages)

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The purpose of this study was to develop chitosan-based porous semi-interpenetrating polymer network(semi-IPN) for gastric retention device. Chitosan and glycol chitosan hydrogels with numerous pores were prepared, and their swelling behaviors in acidic solution were studied. And, to improve mechanical strength of hydrogels, PEO and PVA were used in IPN synthesis with glycol chitosan. Swelling studies were performed in different pH solution at 37°C. Morphology and pore size of hydrogels were confirmed by scanning electron microscopy(SEM). Mechanical strengths were determined by UTM.

4A-23 : Interplay between Cubic and Hexagonal Phases in Block Copolymer Solutions

저자 : 박문정 , 차국헌

발행기관 : 한국화학공학회 간행물 : 화학공학의 이론과 응용 10권 2호 발행 연도 : 2007 페이지 : pp. 1132-1132 (1 pages)

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The phase behavior of a styrene-isoprene diblock copolymer in a styrene-selective solvent was investigated by in-situ SAXS on isotropic and shear-oriented solutions, and by rheology and birefringence. A remarkable new feature in this phase diagram is the coexistence of both bcc and hcp sphere phases, in a region between close-packed spheres (cps) and hexagonally-packed cylinders (hex). By focusing on the transitions among these various ordered phases during heating and cooling cycles, we observed a strong hysteresis: supercooled cylinders persisted upon cooling. The stability of these supercooled cylinders is quite dependent on concentration and for high concentrations, the supercooled cylinders do not revert to spheres even after quiescent annealing for one month. The spontaneous formation of spheres due to the dissociation of cylinders is kinetically hindered in this case, and the system is apparently not amenable to any pre-transitional fluctuations of cylinders prior to the transition. This contrasts with the case of cylinders transforming to spheres on heating in the melt. Application of large amplitude shear to the supercooled cylinders is effective in restoring the equilibrium sphere phases.

5A-31 : pH-sensitive block copolymer micelle

저자 : 심우선 , 이두성

발행기관 : 한국화학공학회 간행물 : 화학공학의 이론과 응용 10권 2호 발행 연도 : 2007 페이지 : pp. 1133-1133 (1 pages)

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MPEG-PDLLA & MPEG-PCLA block copolymers were synthesized by a ring-opening copolymerization of D,L- lactide and ε-caprolactone with MPEG (monomethoxy poly (ethylene glycol)) as an initiator, respectively. The sulfamethazine was modified with succinic anhydride to conjugate the block copolymer with pH-sensitive moiety (sulfamethazine). Also, the oligo-sulfamethazine(OSM) was obtained by the radical polymerization with transfer agent. The conjugation of the block copolymer and modified sulfamethazine (or oligo-sulfamethazine) was done by the DCC coupling reaction. The micelles of the synthesized pH-sensitive biodegradable block copolymers were characterized by the fluorescence method in the various buffer solutions. Also, the micelle size was certificated by DLS. The critical micelle concentration of pH-sensitive block copolymers decreases with increasing the hydrophobic block length, and increases with increasing the pH of buffer solutions. Also, the pH-sensitive block copolymers show the micelle integration-disintegration due to the pH change at the constant concentration. This pH-sensitive block copolymer makes the drug targeting available, so it is expected to be applied as a superior drug carrier.

6A-33 : Preparation of microcellular polyolefin rubber(Engage)/clay nanocomposite foam in supercritical CO2

저자 : 이동석 , 배성렬 , 장영욱

발행기관 : 한국화학공학회 간행물 : 화학공학의 이론과 응용 10권 2호 발행 연도 : 2007 페이지 : pp. 1134-1134 (1 pages)

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In this study, we successfully generated polyolefin rubber(Engage) microcellular foam by using supercritical carbon dioxide as a physical blowing agent. The Engage/clay nanocomspotie were prepared by blending a organoclay(Closite 20A) with glycidyl methacrylate grafted Engage(Engage 8003) in a Haake internal mixer. From XRD analysis, it was found to form an intercalated nanocomposite. Foaming was performed with the neat Engage and its nanocomposite using supercritical carbon dioxide at 20MPa at 80℃. SEM analysis showed that microcellular foam with cell size in the range of 5 . 20μm were produced.Obviously, the nanocomposite exhibited smaller cell size and large cell density in comparison to neat Engage suggesting that the dispersed silicate particles act as nucleating sites for cell formation.

7A-42 : Micropatterning for Olfactory Bulb and Hippocampus Neuronal Network Structures

저자 : 안동준 , 최혜선

발행기관 : 한국화학공학회 간행물 : 화학공학의 이론과 응용 10권 2호 발행 연도 : 2007 페이지 : pp. 1135-1135 (1 pages)

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The control of neuronal cell adhesion and growth in artificially defined networks in vitro was developed for the study of neuronal signals propagation. Appropriate analysis is still greatly hindered by inherent morphological complexity of cultured neural network without any control in location of and/or synaptic connection between neurons. In order to build neural networks on a substrate, it is required to modify the substrate surface suitable for patterning neurons. Soft contact printing with a polydimethyl-siloxane (PDMS) stamp, which was fabricated by photolithography and molding, was used for controlling selective neuronal cell adhesion and growth. The experimental results indicate that the PDL(cytopilic) pattern stamping on PEG (cytophobic) background is effective in growing the hippocampal neuron cells and olfactory cells selectively along the PDL pattern. To optimize neuron growth and pattern forming neural networks, we counted cell body on node and neurits extended along track. And we traced how the neurons found PDL(cytophilic) tracks using dendrites to locate themselves on tracks.

8구두발표 : 고분자 4 ; A-44 : 광기능 폴리다이아세틸렌 초분자의 Chemical-Chip 응용

저자 : 양두호 , 최현 , 김은진 , 안동준

발행기관 : 한국화학공학회 간행물 : 화학공학의 이론과 응용 10권 2호 발행 연도 : 2007 페이지 : pp. 1136-1136 (1 pages)

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생체막을 모방한 폴리다이아세틸렌(polydiacetylene) 초분자는 온도, 기계적자극, pH 그리고 solvent 등의 외부자극에 의하여 청색(640nm)에서 적색(540nm)으로 색전이된다. 그리고 이러한 특성을 이용하여 cholera toxin, influenza virus, E-coli. 등을 검출할 수 있는 센서에 응용하고자 하는 연구가 진행되어 왔다. 본 연구에서는 DNA 칩이나 단백질 칩과 같이 micro array 형태로 폴리다이아세틸렌 초분자를 NH2로 처리한 유리 기판에 표면 패턴화한다. 표면 패턴화는 NHS와 NH2 사이에 amidization으로 유리 기판에 고정화한다. 패턴화된 유리 기판에 cyclodextrin(CD), polyallylamine (PAA), polyvinylalcohol(PVA), Glucose, vapor phase인 NH3과 HCl을 반응시켰다. CD, PAA, PVA, NH3, HCl는 초분자와 직접, Glucose는 Enzyme을 초분자에 고정화한 후 반응하였다. 그 결과 특정 초분자만 반응하여 색전이 되는 것을 fluorescence로 확인하였다. 그리고 초분자 표면에 ligands를 고정화하여 다양한 물질을 검출 가능할 것이다. 이는 유리 기판상에 센서의 기능을 가지고 있는 다양한 폴리다이아세틸렌 개발에 적용될것이다.

9P-283 : The effect of draw ratio and temperature on the drawing PET sheet

저자 : 문옥길 , 나재식

발행기관 : 한국화학공학회 간행물 : 화학공학의 이론과 응용 10권 2호 발행 연도 : 2007 페이지 : pp. 1137-1137 (1 pages)

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The effect of the double-stage uniaxial drawing process on the mechanical properties of PET sheet was investigated under different drawing conditions. At the double-stage drawing process, the PET sheet samples were stretched at different draw ratio and temperatures. The tensile strengths of each sample were measured on a universial test machine. The tensile strength of PET sheet was increased exponentially as the draw ratio was higher. It had a tendency to be improved by low draw temperature in the low draw ratio range. It showed that at double-stage drawing process, the tensile strength of PET sheet was strongly developed by low temperature and high draw ratio, the double-stage drawing process could be evolved more highly tensile strength of PET sheet than the single-stage drawing process.

10P-284 : Study of the vibration of friction wave on the drawn MoS2-Nylon 6 sheet

저자 : 문옥길 , 나재식

발행기관 : 한국화학공학회 간행물 : 화학공학의 이론과 응용 10권 2호 발행 연도 : 2007 페이지 : pp. 1138-1138 (1 pages)

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Vibration of friction wave of MoS2-filled nylon 6 sheet under high drawing were studied. Drawing MoS2-filled nylon 6 sheet was made by single-stage uniaxial drawing process. Waves of the friction coefficient were measured on a block-on-ring tester where sliding ocurred at a speed of 0.13m/s under 30lb. The curves of friction coefficient of drawing MoS2-Nylon have a similar tendency with non-drawing MoS2-Nylon and pure nylon. Wave vibration relate to the surface condition and hardness. Because that the rough surface of fretting damage behavior was displayed in drawing MoS2-Nylon sheet. This means that the surface of drawing MoS2-Nylon is more rough and hard than non-drawing MoS2-Nylon but tensile strength of drawing MoS2-Nylon is still better.

발행기관 최신논문
자료제공: 네이버학술정보
발행기관 최신논문
자료제공: 네이버학술정보

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