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한국조직공학·재생의학회> 조직공학과 재생의학> 탈미네랄화된 골분/히알루론산을 이용한 조직공학적 지지체의 제조 및 특성분석

KCI등재SCOUPUS

탈미네랄화된 골분/히알루론산을 이용한 조직공학적 지지체의 제조 및 특성분석

Original Paper : Preparation and Characterization of Demineralized Bone Particle(DBP)/Hyaluronic Acid Hybrid Scaffold

임지예 ( Ji Ye Lim ) , 김순희 ( Soon Hee Kim ) , 소정원 ( Jeong Won So ) , 이종문 ( John M. Rhee ) , 강길선 ( Gil Son Khang )
  • : 한국조직공학·재생의학회
  • : 조직공학과 재생의학 5권3호
  • : 연속간행물
  • : 2008년 09월
  • : 445-450(6pages)
조직공학과 재생의학

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Demineralized bone particle(DBP) that affects to cell proliferation and differentiation has been used to clinical trial as natural material. hyalunonic acid(HA) called natural moisturizer can be used as biomaterials. In this study, we tried to design and characterize novel DBP-HA sponge. DBP-HA sponge were prepared by freeze-drying method after addition of 1 wt% HA solution into fabricated DBP sponge. Sponge was crosslinked with 1-ethyl-(3- 3-dimethyl aminopropyl) carbodiimide hydrochloride(EDC) solution with 50 mM concentration for 24 hrs and lyophilized. We seeded cells in DBP-HA sponge. Cellular attachment was assayed by 3-(4,5-dimethylthiazole-2-yl)- 2,5-diphenyltertazolium-bromide(MTT) test. DBP-HA sponges were characterized by scanning electron microscopy, fourier transeform infrared spectrometer and water ubsorption ability. We demonstrated presence of HA in DBP-HA sponge from C-O functional group observed by the FT-IR analysis. Moreover, we confirmed high water uptake ability and high cell viability in DBP-HA sponge. Therefore, we could expect that DBP-HA scaffolds are applicable for the tissue regeneration.

UCI(KEPA)

I410-ECN-0102-2013-510-002332594

간행물정보

  • : 의약학분야  > 의공학
  • : KCI등재
  • : SCOPUS
  • : 반년간
  • : 1738-2696
  • : 2212-5469
  • : 학술지
  • : 연속간행물
  • : 2004-2015
  • : 718


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1REVIEW ARTICLE : Stem Cell Therapy Status in Veterinary Medicine

저자 : Wo Jong Yang , Soo Jung Lee , Jee Won Yoon , Jeong Ik Lee

발행기관 : 한국조직공학·재생의학회 간행물 : 조직공학과 재생의학 12권 2호 발행 연도 : 2015 페이지 : pp. 67-77 (11 pages)

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The stem cell therapy in veterinary medicine continues to grow both experimentally and clinically. Application of adipose-derived mesenchymal stem cell (MSC), umbilical cord blood-derived MSC, bone marrow-derived MSC and skeletal muscle-derived MSC for treatment of various diseases are currently in use for several species. However, the differential efficacies of various approaches are still being investigated. In stem cell therapy, common disease animal models include mouse, rat, rabbit, swine, canine, caprine, equine and dolphin are used. Whereas for clinical models, canine, equine are dolphin are used. We need attention and participation from the researchers of tissue engineering and regenerative medicine, and now it is necessary to develop mutual understanding and cooperation between veterinary and human medicine fields. This review is focused on some of the recent status of stem cell therapy in the veterinary medicine. Tissue Eng Regen Med 2015;12(Suppl 2):67-77

KCI등재 SCOPUS

2REVIEW ARTICLE : Study of Functional Cosmetics Based on Stem Cell Technology

저자 : Sung Hyun Choi , Ji Soo Yun , Sang Mo Kwon

발행기관 : 한국조직공학·재생의학회 간행물 : 조직공학과 재생의학 12권 2호 발행 연도 : 2015 페이지 : pp. 78-83 (6 pages)

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Considering the steady increase in life expectancy slowing or reversing the deleterious effects of aging has garnered considerable interest. Accordingly, functional cosmetics based on stem cell technology (Stem cell cosmetics), which combine the anti-aging concept with high technology, is an emerging trend in the cosmetics industry. Stem cells possess self-renewal properties and the potency to differentiate. Therefore, stem cells are the most important cells in the skin, as they are the source for continuous regeneration of the epidermis. Stem cell cosmetics are developed based on stem cell technology, which involves using extracts or culture media of stem cells. However, cosmetics containing stem cells or their extracts have not been released into the market due to legal, ethical, and safety concerns. Meanwhile, plant stem cells, which circumvent these problems, are highly regarded in the cosmetics industry for improving culture technology. The European Union prohibits the use of cells, tissues, or products of human origin in cosmetics, whereas the Korea Food and Drug Association has allowed the use of sources originating from stem cell media in cosmetics since 2009. The global cosmetics market is worth more than 242 billion dollars; however, cosmetic companies across the world that are developing and launching stem cell cosmetics based on stem cell activators, culture extracts, and culture media are much more focused on the Korean cosmetic market. Tissue Eng Regen Med 2015;12(Suppl 2):78-83

KCI등재 SCOPUS

3REVIEW ARTICLE : Reevaluation of Spontaneous and Frequently Diagnosed Disease in Companion Animals and Its Application in Tissue Engineering and Regenerative Medicine

저자 : Soo Jung Lee , Ik Jin Jang , Wo Jong Yang , Min Koo , Jin Won Cha , Jee Won Yoon , Kyung Mi Lee , Jeong Ik Lee

발행기관 : 한국조직공학·재생의학회 간행물 : 조직공학과 재생의학 12권 2호 발행 연도 : 2015 페이지 : pp. 84-93 (10 pages)

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In the development of novel tissue engineering and regenerative medicine techniques in humans, preclinical studies using laboratory animal models are required to examine the pathogenic mechanisms and the potential treatment of diseases for the clinics and an indispensable step for determination of their safety and efficacy. Regrettably, the etiological pathology of diseases of spontaneous diseases may not be exactly the same despite the similarities in signs and symptoms observed in artificial laboratory animal models. Therefore, the artificial laboratory animal model is not fully suitable for studies on specific target diseases. However, the animals, especially companion animal, that have spontaneous and frequently occurring diseases due to genetic and environmental factors mostly likely mimic the naive pathology of diseases in humans. Consequently, these species can be better subjects of preclinical studies and veterinary clinical trials in order to possibly gauge the effectiveness of the therapeutic strategies in the clinical stage. Hence, research on the spontaneous and frequently diagnosed diseases of animals predisposed by the type of breeding, age, and gender that are applicable in tissue engineering and regenerative medicine could be an important data for future advancements. In this study, we conducted a survey on the spontaneous and common diseases of canine and feline species to provide a foundation on the effectiveness and practical application of clinical studies. Therefore, this review can contribute significant development in tissue engineering and regenerative medicine in the future. Tissue Eng Regen Med 2015;12(Suppl 2):84-93

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4REVIEW ARTICLE : Evaluation and Application of Cryosectioning in Undecalcified Hard Tissues in Cartilage and Bone Regenerative Medicine

저자 : Seung Hwa Park , Jee Won Yoon , Jae Hyun Park , Bo Young Kim , Judee Grace E Nemeno , Ernesto C Balolong Jr , Yuna Han , Kyung Mi Lee , Soo Jung Lee , Jeong Ik Lee

발행기관 : 한국조직공학·재생의학회 간행물 : 조직공학과 재생의학 12권 2호 발행 연도 : 2015 페이지 : pp. 94-104 (11 pages)

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This article overall describes the development of histological method especially, cartilage and bone research in regenerative medicine. Kawamoto's film method, which has been recently introduced into our laboratory, has been applied in various fields that make sectioning of hard tissues easier than the conventional method. Moreover, this method also does not require the time-consuming chemical fixation and/or decalcification process. Kawamoto method involves the use of the adhesive plastic film instead of a cover glass where the thin tissue sections are attached efficiently at low temperatures (-25°C). Furthermore, the histological method preserves the enzymatic activity in the fresh sections in comparison to that of chemically-treated tissue sections. In fact, we used this Kawamoto's film method in one of our researches in which drug repositioning was employed for cartilage regeneration. Some of the Kawamoto-processed tissue sections are featured in this review article. Therefore, the application of this tissue preparation technique allowed effective and histological and histochemical studies within a shorter preparation time with ease and convenience. In the future, this Kawamoto method may offer further applications in the preparation of a more diverse tissues and samples not only in the preclinical but also in the clinical studies. Tissue Eng Regen Med 2015;12(Suppl 2):94-104

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5Relationships between Structural Stability of Implanted Site and Biomechanical Properties of Tissue-Engineered Cartilage in the Tibiofemoral Joint Defect

저자 : Gang To Lee , Sang Hyug Park , Byoung Hyun Min , Tae Soo Bae

발행기관 : 한국조직공학·재생의학회 간행물 : 조직공학과 재생의학 12권 2호 발행 연도 : 2015 페이지 : pp. 105-112 (8 pages)

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Biomechanical characteristics of articular cartilage in activities of daily life are essential factors to develop tissue-engineered cartilage as a substitution of defect lesion of articular cartilage. However, few studies have explicitly addressed biomechanical characteristics of tissue-engineered cartilage having inferior mechanical material properties. The purpose of this study was to estimate biomechanical characteristics of tissue-engineered cartilage induced in the tibiofemoral joint under compressive load condition using finite element analysis. The two-dimensional axisymmetric finite element model of human tibio-femoral joint was made and five set of partial mechanical properties of tissue-engineered cartilage were applied: Intact (Mo), up 50% (M1.5), down 50% (M0.5), down 90% (M0.1), and down 99.9% (Mx). The results of our study showed that over 50% down in material properties of tissue-engineered cartilage at knee joint were averagely recommended compared to the intact by considering calculated stresses at the contact area between femoral articular cartilage and meniscus. These results could be used as basic data to determine the appropriate mechanical property of the tissue-engineered cartilage for cartilage regeneration. Tissue Eng Regen Med 2015;12(Suppl 2):105-112

KCI등재 SCOPUS

6Effect of Cornus Officinalis Extract on the Differentiation of MC3T3-E1 Osteoblast-like Cells

저자 : Ming Zhen Zheng , Sook Young Lee , Sang Joun Yu , Byung Ock Kim

발행기관 : 한국조직공학·재생의학회 간행물 : 조직공학과 재생의학 12권 2호 발행 연도 : 2015 페이지 : pp. 113-121 (9 pages)

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Cornus officinalis has been widely used as herbal medicine exerting various pharmacological actions such as diuretic, anti-allergic, hypotensive and antibiotic activities. Thus we studied the effect of Cornus officinalis on the proliferation, differentiation and matrix mineralization of MC3T3-E1 osteoblast-like cell line. To do the experiments, Cornus officinalis extract (COE) was prepared using ethanol at different concentrations. The cytotoxic effect and the osteogenic differentiation were examined using MTT assay, alkaline phosphatase (ALP) activity, ALP staining, Alizarin red S staining and reverse transcriptase-polymerase chain reaction for osteogenic differentiation markers such as collagen type I (Col-I), ostoecalcin (OCN). The results indicated that there was no effect on cell proliferation on both 1st and 3rd days after treatment in MC3T-E1 cells. The level of ALP, Col-I mRNA, OCN mRNA and mineralized nodule were significantly higher in COE treated group than no treated group. Our results suggest that the COE promotes the osteoblast differentiation and mineralization without observable cytotoxicity. Therefore, it may play an important role in osteoblastic bone formation, and may possibly to be an effective measure for bone regeneration. Tissue Eng Regen Med 2015;12(Suppl 2):113-121

KCI등재 SCOPUS

7Analysis of Stability of Human Urine Derived Stem Cells during Serial Subcultures

저자 : So Young Chun , Gang Baek Park , Tae Gyun Kwon , Seock Hwan Choi

발행기관 : 한국조직공학·재생의학회 간행물 : 조직공학과 재생의학 12권 2호 발행 연도 : 2015 페이지 : pp. 122-131 (10 pages)

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This study was conducted to assess stabilities of the human urine derived stem cells (USCs) during serial subcultures to demonstrate clinical feasibility for future stem cell therapy. For this work, we compared to cell morphology, proliferation rate, immunophenotype, multi- lineage differentiation potentials and immunomodulatory effect of USCs. USCs maintained cell morphology, cell proliferation ability through serial culture (till passage 7). In immunophenotypical analysis, USCs revealed positive for embryonic/mesenchymal stem cell marker (stage specific embryonic antigen, SSEA4) and mesenchymal stem cell markers (CD44, CD73, CD90 and CD105), while negative for hematopoietic stem cell markers (CD34, CD45 and C-kit) and immunogenic marker (HLA-DR). The USC had multi-differentiation potencies into adipocytes and osteocytes, and these abilities maintained into passage 7. The immunomodulatory effect of USCs with phytohemagglutinin stimulated peripheral blood mononuclear cell proliferation was analyzed with two systems, co-culture or separated culture system. The peripheral blood mononuclear cells' proliferation was effectively inhibited on both systems, and the value was more effective with co-culture system than separate one. The present study suggests that sequential sub-passages till passage 7 could maintain stem cell characteristics, multipotent properties, and immune suppressive effect of USCs which should be considered in their therapeutic application in regenerative medicine. Tissue Eng Regen Med 2015;12(Suppl 2):122-131

KCI등재 SCOPUS

8Bone Formation of Embryonic Stem Cell-Derived Mesenchymal Stem Cells

저자 : Yeon Tae Jung , Ki Yeon Yoo , Hee Su Lee

발행기관 : 한국조직공학·재생의학회 간행물 : 조직공학과 재생의학 12권 2호 발행 연도 : 2015 페이지 : pp. 132-137 (6 pages)

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Human embryonic stem cells are multipotent cells. In this study, We observed osteogenesis of human embryonic stem cell derived mesenchymal stem cells. mbryonic body formation method was used to derive mesenchymal stem cells from human embryonic stem cells. Embryonic stem cell derived mesenchymal stem cells were immunostained for CD 73 to make characterization of mesencymal stem cells. Osteogenesis of CD 73 positive mesencymal stem cells with media included ascorbic acid, dexamethasone and glycerophosphate was induced. After 10 days culture with osteogenesis media, the cells were immunostained with type I collagen, osteocalcin and Runx-2. After 21 days culture with osteogenesis media, the cells were stained with alizarin red to observe bone nodule formation. Mesenchymal stem cells were derived from human embryonic stem cells by embryonic body method. After these cells were cultured with osteogenesis media, the cells were differentiated into osteoblast and showed bone nodule formation. Tissue Eng Regen Med 2015;12(Suppl 2):132-137

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9Comparative Study of Recombinant Human Bone Morphogenetic Protein-2 Carriers in Rat Subcutaneous Tissues: Pilot Study

저자 : Sun Kyu Lee , Hyun Min Choi , June Sung Shim , Moon Kyu Chung , Young Bum Park

발행기관 : 한국조직공학·재생의학회 간행물 : 조직공학과 재생의학 12권 2호 발행 연도 : 2015 페이지 : pp. 138-146 (9 pages)

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The purpose of this study was to evaluate and compare the potential of using various biomaterials as a carrier of rhBMP-2 in the subcutaneous layer of rats. 5 subcutaneous defects were made in each of the dorsal surfaces of 10 rats. Then, the rats were divided into the following groups: Control group: sham-surgery control, Experimental group 1: rhBMP-2/Bio-Oss®, Experimental group 2: rhBMP-2/Collatape®, Experimental group 3: rhBMP-2/nCS. Histological and histometrical analysis were performed after the rats were sacrificed at 2 and 4 weeks. Regardless of the type of carrier, the groups treated with rhBMP-2 showed significantly greater bone formation when compared to the control group. The amount of new bone formation was greater at 4 week than 2 week. Total bone formation (%) at 4 week was the greatest in rhBMP/Bio-Oss® (26.76±10.13) group, followed by rhBMP/Collatape® (20.38±5.27) and rhBMP/nCS (4.34±2.58). Within the limitation of this study, the results showed that Bio-Oss®, Collatape®, nanocalcium sulfate all showed new bone formation when used as a carrier of rhBMP-2 in the subcutaneous layer of rats, indicating potential use as carriers for rhBMP-2. Tissue Eng Regen Med 2015;12(Suppl 2):138-146

KCI등재 SCOPUS

10Therapeutic Possibility of Human Fetal Cartilage-Derived Progenitor Cells in Rat Arthritis Model

저자 : Su Jeong Lee , Hyun Ju Oh , Minh Dung Truong , Kyi Beom Lee , Ji Young Kim , Young Jick Kim , So Ra Park , Byoung Hyun Min

발행기관 : 한국조직공학·재생의학회 간행물 : 조직공학과 재생의학 12권 2호 발행 연도 : 2015 페이지 : pp. 147-154 (8 pages)

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Fetal cartilage-derived progenitor cells (FCPCs) are a novel cell source for tissue engineering and cell therapy. Previously, cells from various fetal tissues were shown to have immune-modulatory activity just like mesenchymal stem cells. In this study, we investigated the cytotoxicity and therapeutic possibility of FCPCs as a potential cell therapy for arthritis in vivo. To evaluate the acute toxicity and safety, FCPCs were labeled with PKH-26 and subjected to intra-articular injection in rats. When examined at 1 and 2 weeks, the PKH-26 labeled FCPCs were observed only in the synovial membrane but not in other organs. There were no changes either in the white blood cells, red blood cells, and platelets in the hematological analysis and in the weight of the internal organs at 1 week. Therapeutic effect of FCPCs was examined on the complete Freund's adjuvant (CFA)-induced knee arthritis in rats in comparison with triamcinolone, a representative anti-arthritis drug. When inflammation-related edema of the arthritic knee joint was measured by the knee circumference, it increased significantly from the beginning and maintained until the end of measurement in the CFA group, which decreased back rapidly from 1 day in the triamcinolone group and slowly after 7 days in the FCPCs group. In the histological analysis, both triamcinolone and FCPCs decreased the infiltration of neutrophils and lymphocytes into the arthritic synovial membrane gradually at 3 and 7 days. These results suggest that FCPCs shows low toxicity or immune rejection when injected in the synovial cavity and have a therapeutic potential on the synovial arthritis in the rat model. Tissue Eng Regen Med 2015;12(Suppl 2):147-154

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12008 ISSCR 학회를 통해본 최근 줄기세포 연구 동향

저자 : 서원희

발행기관 : 한국조직공학·재생의학회 간행물 : 조직공학과 재생의학 5권 3호 발행 연도 : 2008 페이지 : pp. 349-350 (2 pages)

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2유전자 조작 줄기세포를 이용한 조직재생

저자 : 이석준 ( Soak Jun Lee ) , 황승태 ( Seung Taeh Hwang ) , 이수홍 ( Soo Hong Lee )

발행기관 : 한국조직공학·재생의학회 간행물 : 조직공학과 재생의학 5권 3호 발행 연도 : 2008 페이지 : pp. 395-403 (9 pages)

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3유도만능세포의 최근 연구동향과 전망

저자 : 박경순 ( Kyung Soon Park )

발행기관 : 한국조직공학·재생의학회 간행물 : 조직공학과 재생의학 5권 3호 발행 연도 : 2008 페이지 : pp. 413-419 (7 pages)

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4자기공명영상법을 이용한 줄기세포 추적 영상

저자 : 김대홍 ( Dae Hong Kim ) , 송지환 ( Ji Hwan Song )

발행기관 : 한국조직공학·재생의학회 간행물 : 조직공학과 재생의학 5권 3호 발행 연도 : 2008 페이지 : pp. 420-424 (5 pages)

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5줄기세포와 노화

저자 : 차혁진 ( Hyuk Jin Cha )

발행기관 : 한국조직공학·재생의학회 간행물 : 조직공학과 재생의학 5권 3호 발행 연도 : 2008 페이지 : pp. 425-429 (5 pages)

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6계대 및 배양액이 슈반세포의 증식과 표현형에 미치는 영향

저자 : 오아영 ( A Young Oh ) , 김순희 ( Soon Hee Kim ) , 정수현 ( Su Hyun Jung ) , 홍현혜 ( Hyun Hye Hong ) , 전나리 ( John M. Rhee ) , 이종문 , 신형식 ( Hyung Sik Shin ) , 강길선 ( Gil Son Khang )

발행기관 : 한국조직공학·재생의학회 간행물 : 조직공학과 재생의학 5권 3호 발행 연도 : 2008 페이지 : pp. 430-436 (7 pages)

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Schwann cell(SCs) has been widely accepted to play indispensable roles during neural development and regeneration but obtaining and culture of SCs is difficulty. SCs have been characterized depending on sequential passages and medium. We measured proliferation and phenotypical stability of SCs using three medium(growth medium, purification medium, high density culture medium). Morphological changes were observed by an optical microscope and cell proliferation was counted using hemacytometer. The effect of sequential passage and medium on the phenotypical stability of SCs was assessed RT-PCR and staining for NF and S-100 as SCs markers. In result, proliferation of SCs showed no difference between three groups up to passage 3. NF and S-100 markers were high expressed in up to passage 3 in PM and HDCM. We concluded that SCs of passage 1, 2 and 3 cultured in HDCM will be useful to cell therapy using SCs or spinal cord regeneration using SCs hybrid scaffold.

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7SIS/PLGA 필름에서 SIS 함량이 디스크세포의 증식에 미치는 영향

저자 : 임지예 ( Ji Ye Lim ) , 김순희 ( Soon Hee Kim ) , 소정원 ( Jeong Won So ) , 최진희 ( Jin Hee Choi ) , 이종문 ( John M. Rhee ) , 신형식 ( Hyung Shik Shin ) , 강길선 ( Gil Son Khang )

발행기관 : 한국조직공학·재생의학회 간행물 : 조직공학과 재생의학 5권 3호 발행 연도 : 2008 페이지 : pp. 437-444 (8 pages)

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Porcine small intestine submucosa is accellular tissue and widely used as a biomaterials without immunorejection responses. Poly(lactide-co-glycilide) (PLGA) has controllable biodegradability and good biocompatibility. In this study, we evaluated 2-dimensional porcine small intestine submucosa (SIS)/PLGA hybrid films on adhesion, proliferation and phenotype maintenance of intervertebral disc cells. We prepared natural/synthetic hybrid films using 0, 10, 20, 40 and 80 wt% of SIS by the solvent evaporation method. SIS/PLGA films were characterized by scanning election microscopy (SEM). Disc cells were seeded on PLGA and SIS/PLGA film and we confirmed the effects of adhesion and proliferation on disc cells according to content of SIS by cell count and SEM observation. Contents of 10 wt% SIS positively affected to proliferate and maintain of disc phenotype in SIS/PLGA Film.

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8탈미네랄화된 골분/히알루론산을 이용한 조직공학적 지지체의 제조 및 특성분석

저자 : 임지예 ( Ji Ye Lim ) , 김순희 ( Soon Hee Kim ) , 소정원 ( Jeong Won So ) , 이종문 ( John M. Rhee ) , 강길선 ( Gil Son Khang )

발행기관 : 한국조직공학·재생의학회 간행물 : 조직공학과 재생의학 5권 3호 발행 연도 : 2008 페이지 : pp. 445-450 (6 pages)

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Demineralized bone particle(DBP) that affects to cell proliferation and differentiation has been used to clinical trial as natural material. hyalunonic acid(HA) called natural moisturizer can be used as biomaterials. In this study, we tried to design and characterize novel DBP-HA sponge. DBP-HA sponge were prepared by freeze-drying method after addition of 1 wt% HA solution into fabricated DBP sponge. Sponge was crosslinked with 1-ethyl-(3- 3-dimethyl aminopropyl) carbodiimide hydrochloride(EDC) solution with 50 mM concentration for 24 hrs and lyophilized. We seeded cells in DBP-HA sponge. Cellular attachment was assayed by 3-(4,5-dimethylthiazole-2-yl)- 2,5-diphenyltertazolium-bromide(MTT) test. DBP-HA sponges were characterized by scanning electron microscopy, fourier transeform infrared spectrometer and water ubsorption ability. We demonstrated presence of HA in DBP-HA sponge from C-O functional group observed by the FT-IR analysis. Moreover, we confirmed high water uptake ability and high cell viability in DBP-HA sponge. Therefore, we could expect that DBP-HA scaffolds are applicable for the tissue regeneration.

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9덱사메타손을 함유한 SIS/PLGA 필름의 제조 및 평가

저자 : 이준희 ( Jun Hee Lee ) , 임지예 ( Ji Ye Lim ) , 안식일 ( Sik Il Ahn ) , 박종학 ( Jong Hak Park ) , 김윤태 ( Yun Tae Kim ) , 이종문 ( John M. Rhee ) , 강길선 ( Gilson Khang )

발행기관 : 한국조직공학·재생의학회 간행물 : 조직공학과 재생의학 5권 3호 발행 연도 : 2008 페이지 : pp. 451-455 (5 pages)

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Even though the steroid drug dexamethasone is an efficacious antiinflammatory drug, it is difficult to formulate in an injectable formulation because of its poorly water solubility. So, polymeric film containing dexamatasone was designed drug delivery system including small intestinal submucosa (SIS) and sustained release of the drug from the polymeric system. poly(D,L-lactide-co-glycolide)(PLGA) is used for polymeric film that has been widely used as carriers in controlled release delivery system due to its biodegradability and relatively good biocompatibility. Also, the release behavior of dexamethasone on film controlled by adding the additive such as SIS. SIS derived from the submucosa layer of porcine intestine has been widely used as biomaterial due to low immune response and biocompatibility. SIS/PLGA film were manufactured by the method of solvent and characterized by scanning election microscopy (SEM). In vitro release behavior measured by HPLC and the PLGA molecular weight according to degradation of PLGA analyzed by GPC and pH meter. In this study, the films containing natural materials such as SIS according to content showed zero-order release profile. It was confirmed that the release of dexamethasone form films could be easily controlled by adding SIS.

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10세포-PLGA 쉬이트를 사용한 대망배양

저자 : 김진훈 ( Jin Hoon Kim ) , 김진국 ( Jhin Gook Kim ) , 서수원 ( Soo Won Seo )

발행기관 : 한국조직공학·재생의학회 간행물 : 조직공학과 재생의학 5권 3호 발행 연도 : 2008 페이지 : pp. 456-459 (4 pages)

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The cell and porous scaffold are main components of tissue engineering. The cell seeded-porous scaffold is cultured in vitro to make tissue-like structure and then transplanted into in vivo to reconstruct the injured tissue and organ. However, in vitro culture method can not supply sufficient blood vessels and nutrients and growth factors for tissue formation and also require long cell culture time. For these reasons, in vivo culture method using omentum has been studied, because the omentum can supply many blood vessels and various nutrient and oxygen. But once the porous scaffold transplanted into in vivo, the intrusion of other kinds of cells into scaffold pores and the excessive accumulation of extracellular matrix are inevitable. As a result merits of the use of omentum are not worth. To avoid these problems and use omentum effectively, stable cell layer composed with cells have to be made. In this experiment, we made stable cell layer using PLGA sheet and used Q dot for identification of in vivo cell distribution.

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