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한국생약학회> Natural Product Sciences> Methanol Extract of Longanae Arillus Regulates Sleep Architecture and EEG Power Spectra in Restraint-Stressed Rats

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Methanol Extract of Longanae Arillus Regulates Sleep Architecture and EEG Power Spectra in Restraint-Stressed Rats

Yuan Ma , Jae Soon Eun , Kwang Seung Lee , Eun Sil Lee , Chung Soo Kim , Bang Yeon Hwang , Ki Wan Oh
  • : 한국생약학회
  • : Natural Product Sciences 15권4호
  • : 연속간행물
  • : 2009년 11월
  • : 213-221(9pages)
Natural Product Sciences

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Longanae Arillus (the rind of fruits of Dimocarpus longan) has been consumed for the treatment of insomnia and anxiety in Asia. To provide further scientific basis to traditional uses of this fruit on insomnia, we evaluated the effects of methanol extract of Longanae Arillus (MELA) on the alteration of sleep architecture and electroencephalogram (EEG) power spectra in acutely and chronically restraint-stressed rats. Following postsurgical recovery, Polygraphic signs of sleep-wake activities were recorded for 24 h after MELA administration in rats. Rats in the acute stress and chronic stress were administered with MELA for 10 days. On the 8th, 9th and 10th day of MELA administration, the rats were stressed for 3 h once per day. On the 10th day and 1 h after MELA administration, the rats were stressed once for 22 h in the chronic stress group. Acute and chronic stress induced alternations in cortex EEG recordings during non-rapid eye movement (NREM), rapid eye movement (REM) sleep and wakefulness. MELA shortened the total and REM sleep and increased the wakefulness in night time recording without changing daytime recordings. Chronic stress increased wakefulness and REM sleep, decreased total and NREM sleep in the daytime recording, and increased REM and decreased NREM sleep without changing total sleep and wakefulness in night time recording. These findings suggest that MELA ameliorated the alterations in REM and NREM sleep of acutely and chronically stressed rats via modulation of cortical α-, θ- and δ- wave activity.

UCI(KEPA)

I410-ECN-0102-2012-510-001196769

간행물정보

  • : 의약학분야  > 약화학
  • : KCI등재
  • : SCOPUS
  • : 계간
  • : 1226-3907
  • : 2288-9027
  • : 학술지
  • : 연속간행물
  • : 1995-2022
  • : 1214


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1In-vitro Antimalarial Investigations and Molecular Docking Studies of Compounds from Trema orientalis L. (blume) Leaf Extract

저자 : Babatunde Bolorunduro Samuel , Wande Michael Oluyemi , Ayoyinka Oluwaseun Okedigba

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 28권 2호 발행 연도 : 2022 페이지 : pp. 45-52 (8 pages)

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The identification of Plasmodium falciparum enoyl acyl-carrier protein reductase (pfENR) is considered as a potential biological target against malaria. Trema orientalis is considered a rich source of phytochemicals useful in malaria treatment. This study evaluated the in-vitro inhibitory activity of the extract and isolated compounds of T. orientalis leaf; the isolated compounds and the analogues of the most active compound were subjected to in-silico molecular docking studies on pfENR. The methanolic extract of T. orientalis was subjected to repeated chromatographic separation which led to the isolation of some compounds. The isolated compounds from the plant were examined for their antimalarial activity using β-hematin inhibition assay. Virtual screening via molecular docking and ADMET studies were conducted to gain insight into the mechanism of binding of ligand and to identify effective pfENR inhibitors. The isolated compounds and the analogues of the most active isolates were gotten from PubChem library for use in docking study. Hexacosanol and β-sitosterol showed inhibition of the β-hematin formation. The docking results showed that hexacosanol, β-sitosterol and the analogues of β-sitosterol displayed binding energy ranging between -6.1 kcal/mol and -11.6 kcal/mol. Sitosterol glucoside has the highest docking score. Some of the ligands showed more binding affinity than known bioactive compounds used as reference. Analogues of β-sitosterol has been shown to be potential inhibitors of pfENR, therefore, the findings from this study suggest that sitosterol glucoside and ergosterol peroxide could act as antimalarial agents after further lead optimisation investigations.

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2A New Dimeric Lignan from the Stems of Willughbeia edulis

저자 : Hai Xuan Nguyen , Truong Nhat Van Do , Tho Huu Le , Phu Hoang Dang , Mai Thanh Thi Nguyen , Nhan Trung Nguyen

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 28권 2호 발행 연도 : 2022 페이지 : pp. 53-62 (10 pages)

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As part of our continued study on the chemical constituents of Willughbeia edulis stems, a new dimeric lignan named edulignan (1) was isolated from its EtOAc-soluble extract. Based on NMR spectroscopic interpretation, the planar structure of 1 has been suggested to have two 2-substituted 4-chromanone subunits with different stereochemical configurations. In addition, the MS/MS analysis of the products obtained by acidcatalyzed hydrolysis of 1 was supportive of its structure. Unfornatually, the new compound 1 did not show α- glucosidase inhibitory activity with an IC50 value > 250 μM.

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3Two New Flavanones from the Leaves of Flemingia lineata (L.) Aiton

저자 : Mulyadi Tanjung , Tjitjik Srie Tjahjandarie , Shola Mardhiyyah , Ghinsha Zakatina Rahman , Muhammad Fajar Aldin , Ratih Dewi Saputri , Norizan Ahmat

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 28권 2호 발행 연도 : 2022 페이지 : pp. 58-71 (14 pages)

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Three isoprenylated flavanones were isolated from the leaves of Flemingia lineata (L.) Aiton. Among them are two new flavanones, flemilineatins A and B (1 - 2), along with 6-isoprenyl eridioctyol (3). Their structures were determined using HRESIMS data and NMR spectra. Flavanones 1 - 3 were assayed in the HeLa cancer cells. Compound 1 showed moderate activity with an IC50 value of 11.2 μM.

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4Chemical Profile and Cytotoxicity Activity of Stem-bark of Anacardium occidentale

저자 : Abdullahi Shehu , Mangala Gowri Ponnapalli , Mohd Mahboob , Pitta Venkata Prabhakar , Gabriel Ademola Olatunji

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 28권 2호 발행 연도 : 2022 페이지 : pp. 62-88 (27 pages)

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Column chromatographic fractionation of the methanol and ethyl acetate extracts of the stem-bark of Anacardium occidentale led to the isolation of five compounds (1-5). Their structures were determined by spectroscopic means by comparing spectral data to be β-sitosterol (1), 2,4-dihydroxy acetophenone (2), 1- monolinolein (3), ethyl oleate (4) and β-sitosterol-3-O-β-D-glucopyranoside (5). These compounds were evaluated for cytotoxicity against human cancer cell lines: A549, SCOV3 and rat normal cell line NRK49f. Compounds 2-5 were for the first time isolated from A. occidentale.

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5Standardized Extract (HemoHIM) Ameliorated High Intensity Exercise Induced Fatigue in Mice

저자 : Hyun Jun Lee , Sang Back Kim , Kyung Jun Boo , Darlene Mae Ortiz , Leandro Val Sayson , Raly James Perez Custodio , Jae Hoon Cheong , Seul Ki Kim , Mikyung Kim , Hee Jin Kim

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 28권 2호 발행 연도 : 2022 페이지 : pp. 68-74 (7 pages)

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HemoHIM was used as a Korean traditional medicine for anti-inflammatory and antioxidant effects. However, there is no study on the effect of HemoHIM on fatigue. We examined the potential use of HemoHIM to determine whether it can induce anti-fatigue effects. Mice were administered with HemoHIM and VEH for 14 days. On the last day of treatment, mice were subjected to behavioral tests. Subsequently, their plasma and muscle were collected after the treadmill test to measure lactate, lactate dehydrogenase (LDH), ammonia, corticosterone, glycogen, and creatine kinase (CK). We found that HemoHIM moderately increased the running time (s) in the treadmill and mobility duration in the cold swimming tests. In addition, the VEH group showed a significant increase in lactate, LDH, and corticosterone levels in the plasma compared to the group that did not perform the test. However, this was moderately reduced in HemoHIM treatment. Moreover, the HemoHIMtreated group showed significant differences in LDH and glycogen levels, and showed significantly different CK levels in the muscle. HemoHIM is considered to be effective in improving fatigue, given the duration of cold swimming or running time on a treadmill. Also, HemoHIM treatment resulted in reduced concentrations of blood and muscle parameter analysis.

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6A Comparison of Phenolic Components in Cinnamon Medicines

저자 : Chae Won Kim , Jun Hwi Ko , Do Hyeong Kim , Dezhong Jin , Sung Kwon Ko

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 28권 2호 발행 연도 : 2022 페이지 : pp. 75-79 (5 pages)

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As a result of comparing the phenolic components of cinnamon medicines, the total phenolic component content of Cinnamomi Cortex in China was about 2.65 times higher than that of Cinnamomi Cortex in Vietnam. In addition, the total phenolic component content of Vietnamese Cinnamomi Cortex Spissus was about 1.80 times higher than that of Chinese Cinnamomi Cortex Spissus. Meanwhile, Vietnamese Cinnamomi Ramulus showed a content about 3.29 times higher than that of Chinese Cinnamomi Ramulus. Cinnamaldehyde, the main component of cinnamon medicines, showed the same tendency as the total phenolic component content. In terms of the average content of the total phenolic components, Cinnamomi Cortex showed the highest content at 23964 μg/g, followed by Cinnamomi Cortex Spissus at 17489 μg/g and Cinnamomi Ramulus at 5435.8 μg/g. These results showed that Cinnamomi Cortex and Cinnamomi Cortex Spissus with stem bark as usage sites had about 3.22 to 4.41 times higher content of phenolic components than Cinnamomi Ramulus with young branches as usage sites.

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7Anti-metastatic Effect of Natural Product-motivated Synthetic PPAR-γ Ligands

저자 : Dan-dan Li , Ying Wang , Zhiran Ju , Eun La Kim , Jongki Hong , Jee H. Jung

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 28권 2호 발행 연도 : 2022 페이지 : pp. 80-95 (16 pages)

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Colorectal cancer is one of the most common cancers globally, ranking second for the number of cancer-related deaths. Metastasis has been reported as the main cause of death in patients with colorectal cancer. Peroxisome proliferator-activated receptor gamma (PPAR-γ) is a transcription factor that functions as a tumor suppressor by inhibiting cellular proliferation, migration, and invasion. In our previous efforts to generate natural product-motivated PPAR-γ ligands, the compounds 1 and 2 were obtained. These compounds activated PPAR-γ and inhibited the migration and invasion of HCT116 colorectal cancer cells, and they were also found to inhibit the epithelial-to-mesenchymal transition, which is a key process in cancer metastasis. Compounds 1 and 2 upregulated expression of the epithelial marker (E-cadherin), and downregulated expression of the mesenchymal marker (N-cadherin) and transcriptional factor (Snail). Therefore, the PPAR-γ agonists 1 and 2 could serve as a valuable model for the study on anti-metastatic leads for the treatment of colorectal cancer.

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8Effect of Puffing in the Extraction of Active Ingredients from the Roots of Paeonia lactiflora and Astragalus membranaceus

저자 : Hyojin Lee , Kyoung Won Jang

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 28권 2호 발행 연도 : 2022 페이지 : pp. 89-92 (4 pages)

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In Asia, the roots of Paeonia lactiflora and Astragalus membranaceus have been used as therapeutic agents for thousands of years. Once the medicinal plants are harvested, they are dried and their ingredients are extracted by heat-mediated reflux extraction. However, the condensed structure of organic products (especially roots) limits the extraction of bioactive components. In this study, we assessed the effect of the puffing method (using high temperature and pressure) before the extraction process in relation to the profile and antioxidant capacity of active ingredients. We demonstrated that the additional puffing process before extraction methods improves the yield of polyphenol concentrations and antioxidant activities from the roots of P. lactiflora and A. membranaceus.

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1Phytochemical Constituents from the Seeds of Lithospermum erythrorhizon

저자 : Jun Yeon Park , Sul Lim Lee , Saem Han , Hye Min Kim , Jeong Min Lee , Young Hee Ahn , Sook Young Lee , Sang Hyun Lee

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 15권 4호 발행 연도 : 2009 페이지 : pp. 181-184 (4 pages)

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Phytochemical constituents were isolated from the seeds of Lithospermum erythrorhizon through open column chromatography and prep-HPLC. Their structures were identified as β-sitosterol (1), daucosterol (2), luteolin (3), and allantoin (4) on the basis of spectroscopic analysis. Among them, luteolin (3) was isolated for the first time from the plant.

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2Aqueous Extract of Schizandra chinensis Suppresses Dextran Sulfate Sodium-induced Generation of IL-8 and ROS in the Colonic Epithelial Cell Line HT-29

저자 : Young Mi Lee , Kang Soo Lee , Dae Ki Kim

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 15권 4호 발행 연도 : 2009 페이지 : pp. 185-191 (7 pages)

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Intestinal epithelial cells (IEC) play an important role in the mucosal immune system. IEC-derived mediators of inflammatory cascades play a principal role in the development of colon inflammation. The aim of this study was to investigate the inhibitory effect of aqueous extracts of Schizandra chinensis fruits (SC-Ex) on the production of inflammatory mediators by the human colonic epithelial cells. HT-29 cells were stimulated with dextran sulfate sodium in the presence or absence of SC-Ex to examine the cytoprotection and production of IL-8 and reactive oxygen species (ROS). It was shown that dextran sulfate sodium (DSS) caused the reduction of cell viability and production of IL-8 and ROS in DSS-treated HT-29 cells. We observed that the treatment of SC-Ex protected significantly cell proliferation from DSS-induced damage in dose-dependent manner. SC-Ex (10 and 100 μg/ml) also suppressed DSS-induced production of IL-8 mRNA and protein. Moreover, DSS-induced ROS production was inhibited markedly by the treatment of 100 μg/ml SC-Ex. These results suggest that SC-Ex has the protective effects on DSS-induced cell damage and the release of inflammatory mediators in the intestinal epithelial cells.

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3Chemical Constituents from Artemisia iwayomogi Increase the Function of Osteoblastic MC3T3-E1 Cells

저자 : Yan Ding , Chun Liang , Eun Mi Choi , Jeong Chan Ra , Young Ho Kim

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 15권 4호 발행 연도 : 2009 페이지 : pp. 192-197 (6 pages)

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Chemical investigation of the aerial parts of Artemisia iwayomogi has afforded five glycoside compounds. Their chemical structures were characterized by spectroscopic methods to be turpinionoside A (1), (Z)-3-hexenyl O-α-arabinopyranosyl-(1→6)-O-β-D-glucopyranoside (2), (Z)-5`-hydroxyjasmone 5`-O-β-D-glucopyranoside (3), (-)-syringaresinol-4-O-β-D-glucopyranoside (4), and methyl 3,5-di-O-caffeoyl quinate (5). All of them were isolated for the first time from Artemisia species. The effect of compounds 1-5 on the function of osteoblastic MC3T3-E1 cells was examined by checking the cell viability, alkaline phosphatase (ALP) activity, collagen synthesis, and mineralization. Turpinionoside A (1) significantly increased the function of osteoblastic MC3T3-E1 cells. Cell viability, ALP activity, collagen synthesis, and mineralization were increased up to 117.2% (2 μM), 110.7% (0.4 μM), 156.0% (0.4 μM), and 143.0 % (2 μM), respectively.

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4Effects of α-Asarone against Global Cerebral Ischemia in Rats

저자 : Young Ock Kim , Young Soo Kwon , Seong Eun Lee , Sang Won Lee , Hee Jae Lee

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 15권 4호 발행 연도 : 2009 페이지 : pp. 198-202 (5 pages)

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Based on the use of Acorus gramineus SOLAND (AG) for the treatment of stroke in traditional Korean medicine, the present study was carried out to evaluate neuroprotective effects of α-asarone after transient global cerebral ischemia using rat 4-vessel occlusion (4VO) model in rats. α-Asarone (5 mg/kg) administered intraperitoneally significantly protected CA1 neurons against 10 min transient forebrain ischemia as demonstrated by measuring the density of neuronal cells stained with Cresyl violet. α-Asarone significantly reduced hippocampal neuronal cell death by 85.2% where as its isolated single compounds from AG compared with a vehicle-treated group.

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5Minor Phenolic Constituents of the Anemarrhenae Rhizoma

저자 : Ui Joung Youn , Ye Seul Lee , Ha Na Jeong , Joo Won Nam , Yoo Jin Lee , Young Min Son , Eun Sook Hwang , Eun Kyoung Seo

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 15권 4호 발행 연도 : 2009 페이지 : pp. 203-207 (5 pages)

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A homoisoflavanone, 7,4`-dihydroxyhomoisoflavanone (1) and a flavanone, (2S)-7,4`-dihydroxy-5-methoxyflavanone (2), were isolated from the rhizomes of Anemarrhena asphodeloides, together with 4,4`-dihydroxychalcon (3), 2`-O-methylphlorethin (4), 1,3-bis-di-p-hydroxyphenyl-4-penten-1-one (5), and 2,4`-dihydroxy-4-methoxybenzophenone (6) on the basis of spectroscopic and physicochemical analyses including 1D-and 2D-NMR techniques as well as by comparison of their data with the published values. Compounds 1-4 were isolated for the first time from this plant source. Among isolates, compound 2 exhibited moderate inhibitory effect on the differentiation of pre-adipocyte 3T3-L1 cells.

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6Quantitative Analysis of Fustin and Sulfuretin in the Inner and Outer Heartwoods and Stem Bark of Rhus verniciflua

저자 : Min Young Kim , Ill Min Chung , Deog Cheon Choi , Hee Juhn Park

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 15권 4호 발행 연도 : 2009 페이지 : pp. 208-212 (5 pages)

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The heartwood of Rhus verniciflua Stokes (Anacardiaceae) is widely used for a medicinal plant to treat diabetes mellitus and lingering intoxication in the folkloric society of Korea, while the stem bark has been traditionally used to treat menstrual disorder and helminthiasis. We previously reported that a flavonoid, fustin, isolated from the heartwood of R. verniciflua is effective in Alzheimer`s disease and rheumatoid arthritis. To explore the possibility to produce more flavonoid-rich fraction from this plant, the MeOH extracts from the plant parts of stem bark, outer heartwood, and inner heartwood were quantitatively analyzed by HPLC. Phenolic or flavonoid compounds (chlorogenic acid, caffeic acid, p-coumaric acid, sulfuretin, fustin, fisetin, luteolin and astragalin) were detectable in the HPLC chromatogram. The orange-colored inner heartwood was found to contain the highest levels of fustin (16.96 mg/g) and sulfuretin (2.22 mg/g). Moreover, the inner heartwood accumulated fustin and sulfuretin at least 4-fold higher level as compared to the stem bark and outer heartwood. The levels of total phenolic compounds positively correlated with the extents of antioxidant properties. Therefore, the inner heartwood of R. verniciflua could be used to increase fustin concentration of the extract which is capable of treating Alzheimer`s disease and rheumatoid arthritis.

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7Methanol Extract of Longanae Arillus Regulates Sleep Architecture and EEG Power Spectra in Restraint-Stressed Rats

저자 : Yuan Ma , Jae Soon Eun , Kwang Seung Lee , Eun Sil Lee , Chung Soo Kim , Bang Yeon Hwang , Ki Wan Oh

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 15권 4호 발행 연도 : 2009 페이지 : pp. 213-221 (9 pages)

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Longanae Arillus (the rind of fruits of Dimocarpus longan) has been consumed for the treatment of insomnia and anxiety in Asia. To provide further scientific basis to traditional uses of this fruit on insomnia, we evaluated the effects of methanol extract of Longanae Arillus (MELA) on the alteration of sleep architecture and electroencephalogram (EEG) power spectra in acutely and chronically restraint-stressed rats. Following postsurgical recovery, Polygraphic signs of sleep-wake activities were recorded for 24 h after MELA administration in rats. Rats in the acute stress and chronic stress were administered with MELA for 10 days. On the 8th, 9th and 10th day of MELA administration, the rats were stressed for 3 h once per day. On the 10th day and 1 h after MELA administration, the rats were stressed once for 22 h in the chronic stress group. Acute and chronic stress induced alternations in cortex EEG recordings during non-rapid eye movement (NREM), rapid eye movement (REM) sleep and wakefulness. MELA shortened the total and REM sleep and increased the wakefulness in night time recording without changing daytime recordings. Chronic stress increased wakefulness and REM sleep, decreased total and NREM sleep in the daytime recording, and increased REM and decreased NREM sleep without changing total sleep and wakefulness in night time recording. These findings suggest that MELA ameliorated the alterations in REM and NREM sleep of acutely and chronically stressed rats via modulation of cortical α-, θ- and δ- wave activity.

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8Bamboo Culm Extract Downregulated Activation of NKT- and B-cells and Production of IL-6 in Pristane-Induced Lupus Mice

저자 : Byeong Suk Chae , Byung Hyun Park

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 15권 4호 발행 연도 : 2009 페이지 : pp. 222-228 (7 pages)

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Lupus is characterized by immunoregulatory abnormalities between T- and B-cells leading to autoantibody production and multiorgan injuries. We investigated whether bamboo culm extract (BC) ameliorates aberrant activation of T cells and B cells and attenuate production of IL-6 in pristane-induced lupus mice. Lupus was induced by i.p. a single injection of 0.5 ml of pristane in female BALB/c mice, which, later about 4 months, were used as a lupus model. The pristane-induced lupus mice and healthy mice were injected i.p. with BC 5 μl/kg or PBS once a day for 3 weeks. These results demonstrated that BC significantly decreased levels of serum and BAL IL-6 and production of IL-6 by macrophages with/without LPS, and downregulated expression of NKT cell and CD86+ CD45R/B220+, but not CD80+CD45R/B220+ and CD69+CD4+ in the splenocytes in pristine-induced lupus mice. Moreover, BC greatly increased Con A-stimulated production of IL-6, IL-10 and IFN-γ by splenocytes obtained from pristane-induced lupus mice. Therefore, our findings suggest that BC may ameliorate lupus pathogenesis in pristane-induced lupus mice via downregulation of aberrant activation of NKT cells and B cells and inhibition of production of IL-6.

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9In Vitro Anti-Inflammatory Effects of the Essential Oil of Artemisia iwayomogi and Its Main Component, Vulgarone B

저자 : Eun Young Chung , Seung Won Shin

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 15권 4호 발행 연도 : 2009 페이지 : pp. 229-233 (5 pages)

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The essential oil fraction was obtained from the leaves and flowers of Artemisia iwayomogi (Compositae) by steam distillation, and its main component, vulgarone B, was isolated by column chromatography. RAW 264.7 cells were used to investigate the anti-inflammatory properties of A. iwayomogi and vulgarone B. Cell viability was determined by MTT assay after treatment with various dilutions of the compounds. In addition, several assays were used to determine the effects of A. iwayomogi essential oil components on immune stimulation. Nitric oxide production in cells activated with lipopolysaccharide (LPS) was evaluated by reaction with Griess reagent. Both vulgarone B and the essential oil fraction of A. iwayomogi inhibited the production of nitric oxide. The effects on various cytokines released from the cells were also measured using ELISA. The production of prostaglandin E2 was significantly decreased by treatment with A. iwayomogi oils. LPS-induced IL-1β and IL-6 production were also decreased in a dose-dependent manner, but no significant effect on TNF-α was observed at the concentrations tested. Finally, Western blot analysis revealed that A. iwayomogi oils reduced the levels of COX-2 and iNOS.

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10Phytochemical Constituents of Bistorta manshuriensis

저자 : Sang Wook Chang , Ki Hyun Kim , Il Kyun Lee , Sang Un Choi , Shi Yong Ryu , Kang Ro Lee

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 15권 4호 발행 연도 : 2009 페이지 : pp. 234-240 (7 pages)

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Phytochemical investigation of the MeOH extract of the aerial parts of Bistorta manshuriensis resulted in the isolation of two cerebrosides, two lactams, six phenolic compounds and seven flavonoids. Their chemical structures were characterized by spectroscopic methods to be pinelloside (1), soyacerebroside I (2), pterolactam (3), 5-hydroxypyrrolidine-2-one (4), vanillic acid (5), caffeic acid methyl ester (6), protocatechuic acid (7), caffeic acid (8), 3,5-di-O-caffeoyl quinic acid methyl ester (9), chlorogenic acid methyl ester (10), avicularin (11), afzelin (12), quercetin (13), isoorientin (14), quercetin 3-O-β-D-glucoside (15), quercitrin (16), and luteolin (17). The isolated compounds (1-4, 7, 12, 14) were isolated for the first time from this plant source and the compounds 1-4, 9 and 10 were first reported from the genus Bistorta. Compound 17 exhibited moderate cytotoxicity and compound 6 exhibited weak cytotoxicity against four human cancer cell lines in vitro using an SRB bioassay.

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