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대한산부인과학회> Obstetrics & Gynecology Science> 자궁내막증환자에서 Western blot 과 효소면역법에 의한 순환 항자궁내막항체 측정

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자궁내막증환자에서 Western blot 과 효소면역법에 의한 순환 항자궁내막항체 측정

Determination of Circulating Antiendometrial Antibodies in Patients with Endometriosis by Western Blot and Enzyme - linked Immunosorbent Assay

문신용(Shin Yong Moon) , 최영민(Young Min Choi) , 김석현(Seok Hyun Kim) , 이진용(Jin Yong Lee) , 김정구(Jung Gu Kim) , 강순범(Soon Beom Kang) , 서창석(Chang Seok Suh)
  • : 대한산부인과학회
  • : Obstetrics & Gynecology Science 42권3호
  • : 연속간행물
  • : 1999년 03월
  • : 475-480(6pages)
Obstetrics & Gynecology Science

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Objectives: To investigate whether endometrial proteins with molecular weight(MW) of 92 kilodalton(kDa) may be a specific antigen involved in autoimmunity in endometriosis and to evaluate the efficacy of enzyme-linked immunosorbent assay(ELISA) in determining antiendometrial antibodies, compared with Western blot. Methods: Sera of forty-eight patients with endometriosis, 21 patients with normal control patients, 7 patients with Mayer-Rokitansky-Kustner-Hauser(MRKH) syndrome and cord sera of 22 male neonates(experimental controls) were tested for the presence of antibodies against endometrial proteins by Western blot and ELISA. All statistics were performed by Fishers exact teast and Student's t-test. Results: Fourteen(29.1%), 18.8%, and 33.3% of sera from patients with endometriosis had immunoglobulin (IgG) antibodies that were reactive against endometrial proteins of MW of 71, 92, 103 kDa while any sera from experimental controls did not show any reactivity against these antigens. Overall, threr were specific IgG antiendometrial antibodies detectable by Western blot in 56.3% of patients with endometriosis and in a normal eontrol patient. The binding activities of serum IgG to endometrial proteins were higher in patients with endometriosis than other groups. Circulating IgG antiendometrial antibodies were detected by ELISA in 54.3% of 35 patients with endometriosis and in 2 normal control patients. The concordance rate between ELISA and Western blot in determining the presence of antiendometrial antibodies was 78.3%. Conclusions: Ninety-two kDa endometrial protein is a specific antigen eliciting IgG responses in endometriosis. ELISA may be an useful method in screening autoimmune endometriosis.

UCI(KEPA)

I410-ECN-0102-2009-510-005330215

간행물정보

  • : 의약학분야  > 산부인과학
  • : KCI등재
  • : SCOPUS
  • : 격월
  • : 2287-8572
  • : 2287-8580
  • : 학술지
  • : 연속간행물
  • : 1958-2022
  • : 11148


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1COVID-19 and vaccination during pregnancy: a systematic analysis using Korea National Health Insurance claims data

저자 : Ki Hoon Ahn , Hae-in Kim , Kwang-sig Lee , Ju Sun Heo , Ho-yeon Kim , Geum-joon Cho , Soon-cheol Hong , Min-Jeong Oh , Hae-joong Kim

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 65권 6호 발행 연도 : 2022 페이지 : pp. 487-501 (15 pages)

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Objective
This study systematically analyzed coronavirus disease 2019 (COVID-19) and vaccination details during pregnancy by using the national health insurance claims data.
Methods
Population-based retrospective cohort data of 12,399,065 women aged 15-49 years were obtained from the Korea National Health Insurance Service claims database between 2019 and 2021. Univariate analysis was performed to compare the obstetric outcomes of pregnant women (ICD-10 O00-O94) and their newborns (ICD-10 P00-P96) with and without COVID-19. Univariate analysis was also performed to compare the age and obstetric outcomes of pregnant women receiving different types of vaccines.
Results
The percentage of pregnant women with COVID-19 during pregnancy was 0.11%. Some obstetric outcomes of pregnant women with COVID-19, including the rates of preterm birth or cesarean delivery, were significantly better than those of pregnant women without COVID-19. The rate of miscarriage was higher in pregnant women with COVID-19 than without COVID-19. However, the outcomes of newborns of women with and without COVID-19 were not significantly different. Regarding vaccination type, obstetric outcomes of pregnant women appeared to be worse with the viral vector vaccine than with the mRNA vaccine.
Conclusion
To the best of our knowledge, this is the first study to systematically analyze COVID-19 and vaccination details during pregnancy using the national health insurance claims data in Korea. The obstetric outcomes in pregnant women with and without COVID-19 and their newborns were similar.

KCI등재 SCOPUS

2Improving body image and sexual life among postpartum women: a single-blind-randomized controlled trial to evaluate a social network-based cognitive-behavioral intervention

저자 : Erfaneh Talebi , Mohammad Heidari , Forouzan Elyasi , Shayesteh Jahanfar , Zohreh Shahhosseini

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 65권 6호 발행 연도 : 2022 페이지 : pp. 502-512 (11 pages)

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Objective
To determine the effect of a social network-based cognitive-behavioral intervention on postpartum body image and sexual life of primiparous women.
Methods
In this single-blind randomized controlled trial, 82 postpartum women were allocated into two intervention and control groups (allocation ratio, 1:1) using permuted block randomization. Primary outcome was change in body image scores between the groups over time. Secondary outcomes were genital self-image, sexual quality of life, and sexual satisfaction. The intervention group participated in eight (2 weeks) 60-minute sessions through WhatsApp (WhatsApp Inc., Mountain View, CA, USA). Data were collected at recruitment, post-intervention, and 1-month of follow-up.
Results
Cognitive-behavioral intervention had a medium effect on improving body image in postpartum women (partial eta squared, 0.33; P<0.001). The results showed significant differences between the two groups regarding sexual quality of life and sexual satisfaction at 1-month follow-up.
Conclusion
The results of this study can be used to develop psychological interventions targeting body image in postpartum women.

KCI등재 SCOPUS

3Preoperative modified frailty index to predict surgical complications in endometrial cancer patients

저자 : Aroontorn Pichatechaiyoot , Sarayut Thannil , Sathana Boonyapipat , Rakchai Buhachat

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 65권 6호 발행 연도 : 2022 페이지 : pp. 513-521 (9 pages)

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Objective
To assess the predictive value of the preoperative modified frailty index (mFI) for postoperative complications in endometrial carcinoma, evaluate risk factors associated with complications, and compare the predictive properties of the mFI with the American Society of Anesthesiologists (ASA) physical status classification.
Methods
A total of 364 patients with endometrial cancer who underwent primary surgery between January 2009 and December 2016 were examined. The prognostic value of mFI in predicting severe postoperative complications, assessed according to the Clavien-Dindo classification, was analyzed and compared with ASA status. The risk factors for adverse outcomes were determined using multivariate analysis.
Results
The 30-day postoperative surgical- or medical-related complication rate was 26.6%. The rates of postoperative complications were 1.3%, 8.8%, 12.2%, and 60.0% for mFI scores of 0, 1, 2, and ≥3, respectively (P<0.001). The odds ratios for predicting postoperative complications in patients with mFI scores of 1, 2, and ≥3 were 7.38, 10.59, and 114.75, respectively. In the multivariate analysis, the significant predictive factors for postoperative complications were mFI ≥1, body mass index (BMI) ≥30 kg/㎡, and non-endometrioid cell type. At cut-off points of mFI ≥1 and ASA ≥2, both tools had similar sensitivities but mFI was more specific (sensitivities 92.9% vs. 100%; specificity 45.5% vs. 19.4%).
Conclusion
mFI provides a satisfactory predictive value for postoperative complications. Patients with an mFI score ≥1, a BMI ≥30 kg/㎡, and a non-endometrioid subtype, are at risk of postoperative complications and should receive comprehensive preoperative and postoperative management.

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4Laparoscopic systemic restaging surgery for women with unexpected uterine malignancy

저자 : Eun Bi Kim , Hyeon Myeong Hong , Won Moo Lee , Joong Sub Choi , Jaeman Bae , Un Suk Jung , Jeong Min Eom , Jihyun Keum

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 65권 6호 발행 연도 : 2022 페이지 : pp. 522-530 (9 pages)

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Objective
We investigated the feasibility of laparoscopic restaging surgery in patients with unexpected uterine cancer.
Methods
This retrospective study included eight patients who underwent laparoscopic restaging surgery for Iran University uterine cancer after a prior hysterectomy or myomectomy.
Results
The median age of the patients and their body mass index were 55 years (range, 44-78) and 23.8 kg/㎡ (range, 20.75-31.89), respectively. The median interval between the prior hysterectomy and the restaging surgery was 21 days (range, 10-35). The median operating time and time for the return of bowel activity were 325 minutes (range, 200-475) and 35 hours (range, 18-50), respectively. The median numbers of harvested pelvic and para-aortic lymph nodes were 17.5 (range, 14-29) and 20.5 (range, 7-36), respectively. In seven of the eight patients, uterine extraction was performed with vaginal or electronic morcellation. The final International Federation of Gynecology and Obstetrics stage was IA in all patients. Intraoperative and postoperative complications did not occur in any of the patients, except for the need for transfusion. Patient 4 had synchronous primary cancer (stage IA) of the endometrium and left ovary. Two of the eight patients with clear cell carcinoma received chemotherapy, and none received radiotherapy. All patients survived without disease recurrence.
Conclusion
Restaging surgery might be necessary for highly selective patients with unexpected uterine malignancies. This would be an alternative surgical modality for complete staging and planning tailored adjuvant treatments. However, lymphadenectomy might not be performed in patients with early uterine cancer.

KCI등재 SCOPUS

5Triphenyl phosphate activates estrogen receptor α/NF-κB/cyclin D1 signaling to stimulate cell cycle progression in human Ishikawa endometrial cancer cells

저자 : Hyun Young Kwon , Seung Bin Park , Myoungseok Han , Jung-woo Park , Yongmin Lee , Sang Jun Han , Youngmin Kwon , Yeon Jean Cho

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 65권 6호 발행 연도 : 2022 페이지 : pp. 531-541 (11 pages)

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Objective
Triphenyl phosphate (TPHP) is one of the most commonly used organophosphorus flame retardants that may accumulate in the environment. However, its effects on human reproductive organs have not been well studied. We aimed to investigate the in vitro effects of TPHP in human Ishikawa endometrial cancer cells to elucidate how TPHP exposure disrupts intracellular signaling and cell proliferation in reproductive tissues.
Methods
Human Ishikawa endometrial cancer cells were exposed to TPHP.
Results
Exposure to TPHP elevated the levels of estrogen receptor (ER) α and progesterone receptor-B and reduced ER β in human Ishikawa endometrial cancer cells. TPHP stimulated phosphoinositide 3-kinase/protein kinase B and mitogen-activated protein kinase/ extracellular signal-regulated kinases 1/2 kinase signaling, which may contribute to the activation of ER function and induce nuclear translocation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in human Ishikawa endometrial cancer cells. Activated ER and NF-κB stimulate the expression of cyclin D1/cyclin-dependent kinase (CDK) 4/CDK6, indicating cell cycle progression and proliferation.
Conclusion
This report may provide new information on the molecular mechanisms underlying how TPHP exposure dysregulates the cellular physiology of the human endometrium.

KCI등재 SCOPUS

6Surgical outcomes of laparoscopic trachelectomy following supracervical hysterectomy: a multicenter study

저자 : Ayman Shehata Dawood , Heba Fouad Harras , Hossam Ramadan Moussa , Ahmed Saber Soliman

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 65권 6호 발행 연도 : 2022 페이지 : pp. 542-551 (10 pages)

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Objective
To evaluate the feasibility, safety, and surgical outcomes of laparoscopic trachelectomy after supracervical hysterectomy.
Methods
This multicenter study was conducted at Tanta University, Benha University, and Aminah Laparoscopy Center (Benha, Egypt) from June 1, 2018 to October 31, 2021. Forty patients were recruited for this study and counseled on laparoscopic trachelectomy to treat their symptoms after supracervical hysterectomy. Furthermore, cervical biopsy was performed to detect and exclude any malignancy. Histopathological examination of cervical specimens was performed after surgery. Operative details and outcomes were recorded.
Results
The median age of the patients was 42 years (range, 38-47). The median body mass index was 25 years (range, 22-28). The median interval between hysterectomy and the clinical presentation was 4.40 years (range, 3.58-5.25). Most patients presented with abnormal vaginal discharge (40%) and bleeding (25%). Moreover, a cervical biopsy result revealed stump carcinoma in three cases (7.5%) that were excluded. The median operative time was 210 minutes (range, 170-220). The median blood loss was 270 mL (range, 220-320). Additionally, histopathological examinations revealed that chronic non-specific cervicitis was present in 54.05% of trachelectomy specimens. There were no significant differences between symptomatic and asymptomatic patients regarding operative outcomes, except adhesions, which were more significantly increased in symptomatic patients (P=0.015). Minimal complications, both operative and postoperative, were related to the procedure.
Conclusion
Although the operative time was long and adhesions were common during laparoscopic trachelectomy, the procedure was feasible and safe, with minimal complications.

KCI등재 SCOPUS

7The effect of diluted ropivacaine in distending fluid on cramping pain after hysteroscopic surgeries: a randomized clinical trial study

저자 : Kobra Tahermanesh , Abolfazl Mehdizadeh Kashi , Marziyeh Ajdary , Samaneh Rokhgireh , Masood Mohseni , Shahla Chaichian , Maryam Ahmadi

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 65권 6호 발행 연도 : 2022 페이지 : pp. 552-559 (8 pages)

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Objective
The effect of ropivacaine on postoperative hysteroscopy has not yet been evaluated; this study investigated the effect of diluted ropivacaine in distending media during hysteroscopy on postoperative cramping pain.
Methods
This double-blind randomized clinical trial was conducted on 60 women who underwent hysteroscopy at a tertiary hospital. Normal saline was used as the distending fluid in both groups. The intervention group received 10 mL of 2% ropivacaine in only one bottle of 1,000 mL normal saline as a distending fluid, while the control group received 10 mL of normal saline in 1,000 mL normal saline during hysteroscopy. Patients' pain scores were evaluated before hysteroscopy and at 2, 6, 12, 24, and 48 hours after hysteroscopy.
Results
Based on the results, the pain measured by visual analog scale (VAS) score was significant at 6 and 12 hours after the intervention was significantly lower than that in the ropivacaine group (3.03±1.57 vs. 4±1.49, P=0.02 at 6 hours and 1.28±1.36 vs. 2.4±1.43, P=0.003 at 12 hours). There were no significant differences in the VAS scores at 2, 24, and 48 hours after the intervention between the two groups.
Conclusion
Ropivacaine in the distending fluid during hysteroscopy is associated with a significant reduction in pain within a few hours after hysteroscopy with no remarkable adverse effects.

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1폐쇄성 무정자증 환자에서 정자 채취 후 난자 세포질내 정자 주입술의 결과 : 결핵성 부고환염의 영향

저자 : 문신용(Shin Yong Moon) , 최영민(Young Min Choi) , 김석현(Seok Hyun Kim) , 오선경(Sun Kyung Oh) , 서창석(Chang Suk Suh) , 이진용(Jin Yong Lee) , 정병준(Byeong Jun Jung) , 김희선(Hee Sun Kim) , 류범용(Buom Yong Ryu) , 김정구(Jung Gu Kim) , 지병철(Byung Chul Jee) , 임경실(Kyung Sil Lim)

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 42권 3호 발행 연도 : 1999 페이지 : pp. 451-456 (6 pages)

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Objective: To investigate the intluence of previous tuberculous epididymitis in infertile males with obstructive azoospermia on the outcome of sperm retrieval and intracytoplasmic sperm injection(ICSI) in IVF-ET propam. Methods: Retrospective analysis was paformed in 26 patients with obstructive azoospermia undergoing sperm retrieval and ICSI at Seoul National University Hospital from January, 1996 to August, 1997; 12 cycles in 5 patients with tuberculous obstructive azoospermia(Group A), and 40 cycles in 21 patients with non-tuberculous obstructive azoospermia(Group B). Results: There was no significant difference in the clinical pregnancy rate(PR) per fresh transfer between Groups A and B(20.0%[2/10] vs. 26.8%(11/41)). The rates of embryo implantation and clinical miscarriage were also comparable in both groups(6.3% vs. 11.1%, 50.0% vs. 9.1%). This tendencies were also similar after including five cryopreserved-thawed transfer cycles. Conclusion: Embryo quality and pregnancy outcome in sperm retrieval and ICSI were comparable in both the tuberculous and non-tuberculous obstructive azoospermia patients. Our results suggest that previous history of tuberculous epididymitis in patients with obstructive azoospermia does not affect the outcome of sperm retrieval and ICSI.

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2고식적 체외수정시술시 3 일째 배아이식술의 효용성에 관한 연구

저자 : 문신용(Shin Yong Moon) , 최영민(Young Min Choi) , 김석현(Seok Hyun Kim) , 오선경(Sun Kyung Oh) , 서창석(Chang Suk Suh) , 이진용(Jin Yong Lee) , 정병준(Byeong Jun Jung) , 김희선(Hee Sun Kim) , 류범용(Buom Yong Ryu) , 김정구(Jung Gu Kim) , 지병철(Byung Chul Jee) , 오수영(Soo Young Oh)

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 42권 3호 발행 연도 : 1999 페이지 : pp. 457-463 (7 pages)

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Objective: To investigate the positive or negative effect of delaying embryo transfer(ET) one day in IVF-ET. Methods: From May to July, 1997, a total of 64 patients was emolled in this prospective randomized case-controlled study. When the timing of oocyte retrieval was decided, random allocation of patients was made to one of the two groups: day 2 transfer or day 3 transfer. In day 3 transfer group, embryos were cultured in M3 media(Medi-Cult) for further 24 hours. Results: There were no significant differences in age of patients, infertility factor, basal serum FSH level, and serum E2 level on hCG day between two poups, but number of previous IVF-ET cycles was significantly higher in day 3 transfer group(p 0.042). Number of oocytes retrieved, fertilization rate, cleavage rate, and number of embryos transferred had no significant difference, but cumulative embryo score(CES) was significantly higher in day 3 transfer group(p 0.0001). Clinical pregnancy and implantation rates were bigher in day 3 transfer group, but without significance(34.4% vs. 21.9%; 8.7% vs, 5.4%). There were also no significant differences in spontaneous miscarriage and multiple pregnancy rates. Especially in patients over 35 years of age, clinical pregnancy and implantation rates were more higher in day 3 transfer group, but without significance(41.7% vs, 8.3%; 8.5% vs. 1.6%). Conclusion: Considering the higher number of previous cycles in day 3 transfer group, it is at least likely that delaying ET one day may be clinically beneficial in IVF-ET, especially in patients with old age or repeated failure of previous cycles.

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3임신 중기 임신중절에서의 Misoprostol의 질내 투여시 사산아와 생존아에서 효능성의 비교

저자 : 김종인(Jong In Kim) , 이정호(Jung Ho Lee)

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 42권 3호 발행 연도 : 1999 페이지 : pp. 464-467 (4 pages)

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Objective: To compare the efficiency, success rate and abortion time between the live and the dead fetus in second trimester pregnancy termination with intravag-inal misoprostol. Subjects and methods: A total of 45 pregnant women between 18-29 weeks of gestation with medical, obstetric, or genetic reasons for termination were recruited to receive 50 ug misoprostol inserted intravaginally (posterior fornix) every 4 hours. Results: The success rate of complete termination(abortion) within 12 and 24 hours in dead fetuses were 78.2% and 95.6%, respectively, while in live fetuses were 36.3% and 90.9%, respectively. The mean abortion time of the dead fetus group (10.31+-3.43 hours) was significantly less than that of the live fetus group (14.20+-3.31 hours). No serious complications occurred in terms of hemorrhage, diarrhea, nausea and vomiting. Conelusion: Intracervicovaginal misoprostol is a safe and effective method for second trimester pregnancy termination. The abortion time is less in dead fetus pregnancy than that in the live fetus pregnancy.We used 50 ug tablets of misoprastol every four hours. But, we suspect that the regimen of 100ug misoprostol inserted intracervicovaginally every eight hours will beis the proper and optimal method for pregnancy termination.

KCI등재SCOUPUS

4원발성 난관암의 임상병리학적 연구

저자 : 목정은(Jung Eun Mok) , 조준식(Jun Sik Jo) , 나준희(Jun Hee Na) , 김종혁(Jong Hyeok Kim) , 김봉희(Bong Hee Kim) , 허주령(Joor Rung Huh) , 김용만(Yong Man Kim) , 김영탁(Young Tak Kim) , 남주현(Joo Hyun Nam)

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 42권 3호 발행 연도 : 1999 페이지 : pp. 468-474 (7 pages)

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Objective The aim of this study was to evaluate the clinicopathologic findings of eight patients with primary cancer of fallopian tube diagnosed and treated in the department of Obstetrics and Gynecology, University of Ulsan, Asan Medical Center for nine years. Methods Demographic characteristics, symptoms, signs, stage, tumor grade, mode of therapy and survival of patients were reviewed retrospectively. Results The patients with primary cancer of fallopian tube constituted 0.4% of all gynecologic malignancies encountered during this period. The age of patients ranged 42 to 70 years(mean+- S.D.; 53.9+-9.9) and half of patients were postmenopausal and two patients were nulliparous. Patients were treated by simple or radical hysterectomy and bilateral salpingoophorectomy with or without omentectomy and five patients received postoperative chemotherapy. The FIGO surgical stages of the patients were I(4 cases), II(2 cases), III(1 case) and IV(1 case). There were 3 cases of adenocarcinoma, 3 cases of malignant mixed Mullerian tumor, 1 case of undifferentiated carcinoma and 1 case of serous cystic tumor of low malignancy potential. Serum CA 125 values were followed during treatment and decreased during clinical remission and increased at the time of tumor progression. The mean follow-up duration was 24.3(+28.3) months and 7 patients were alive free of disease and one patients with stage Ic died of the disease. Conclusion Primary fallopian tube cancer is an extremely rare malignancy of the female genitalia and preoperative diagnosis of this disease is difficult. Cytoreductive surgery and postoperative combination chemotherapy seem to be effective treatment and CA 125 value could reflect the disease status during the treatment and follow-up of patients.

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5자궁내막증환자에서 Western blot 과 효소면역법에 의한 순환 항자궁내막항체 측정

저자 : 문신용(Shin Yong Moon) , 최영민(Young Min Choi) , 김석현(Seok Hyun Kim) , 이진용(Jin Yong Lee) , 김정구(Jung Gu Kim) , 강순범(Soon Beom Kang) , 서창석(Chang Seok Suh)

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 42권 3호 발행 연도 : 1999 페이지 : pp. 475-480 (6 pages)

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Objectives: To investigate whether endometrial proteins with molecular weight(MW) of 92 kilodalton(kDa) may be a specific antigen involved in autoimmunity in endometriosis and to evaluate the efficacy of enzyme-linked immunosorbent assay(ELISA) in determining antiendometrial antibodies, compared with Western blot. Methods: Sera of forty-eight patients with endometriosis, 21 patients with normal control patients, 7 patients with Mayer-Rokitansky-Kustner-Hauser(MRKH) syndrome and cord sera of 22 male neonates(experimental controls) were tested for the presence of antibodies against endometrial proteins by Western blot and ELISA. All statistics were performed by Fishers exact teast and Student's t-test. Results: Fourteen(29.1%), 18.8%, and 33.3% of sera from patients with endometriosis had immunoglobulin (IgG) antibodies that were reactive against endometrial proteins of MW of 71, 92, 103 kDa while any sera from experimental controls did not show any reactivity against these antigens. Overall, threr were specific IgG antiendometrial antibodies detectable by Western blot in 56.3% of patients with endometriosis and in a normal eontrol patient. The binding activities of serum IgG to endometrial proteins were higher in patients with endometriosis than other groups. Circulating IgG antiendometrial antibodies were detected by ELISA in 54.3% of 35 patients with endometriosis and in 2 normal control patients. The concordance rate between ELISA and Western blot in determining the presence of antiendometrial antibodies was 78.3%. Conclusions: Ninety-two kDa endometrial protein is a specific antigen eliciting IgG responses in endometriosis. ELISA may be an useful method in screening autoimmune endometriosis.

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6전치 태반의 임상적 고찰

저자 : 김수평(Soo Pyung Kim) , 이초희(Cho Hi Lee) , 김사진(Sa Jin Kim) , 허수영(Soo Young Hur) , 이귀세라(Gui Se Ra Lee) , 이지현(Jee Hyun Lee) , 권인(In Kweon) , 송승규(Seung Kyu Song)

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 42권 3호 발행 연도 : 1999 페이지 : pp. 481-486 (6 pages)

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Objective: A Clinical Study of Placenta P#revia done by cesarean section and the study of preterm delivery that is major cause of premature motality even thought expected managment of placente previa. Methods: This study was evaluated for the clinical analysis on the 641 patients with placenta previa among total deliveries of 56,120 cases at the St. Mary and Holy Family hospital of Catholic University from Jan. 1,1989 to Dec.31,1997. Results: The following results were obtained: 1. The total incidence of placenta previa was 1.14%. 2. The incidence of placenta previa increases with age. 3. Placenta previa has occurred more often in multipara(60.2%) than primipara(39.8%), and placenta previa has occurred more often in women who had experienced abortion(71.8%) than women who hadnt(28.2%). 4. The types of placenta previa distributed 326 cases of totalis(50.9%), 110 cases of partialis(17.2%), 99 cases of marginal(15.4%) and 106 cases of low lying placenta(16.4%). 5. The fetal presentation distributed 557 cases of vertex(86.9%), 49 cases of breech(7.6%), 28 cases of transverse lie(4.4%). 6. The types of uterine incision included 543 cases of low sepnent transverse incision(84.7%), 46 cases of classical incision(7.2%), 9 cases of inverted T incision and 43 cases of cesarean hysterectomy(6.7%). 7. Premature delivery of placenta previa prior to completion of 37 weeks was 186 cases(29.0%) and low birth infant less than 2,500gm was 128 cases(20.0%).and perinatal mortality rate was 28.0 per 1,000. 8. During delivery, 278 cases of placenta previa(43.4%) were transfused with mean 3.6 pints. Incidence of vaginal bleeding was 49.3% of placenta previa,mean admission day until delivery was about 5 day. Conclusion: there is a strong association between advancing age and incidence of placenta previa, and then. the risk increases with parity & the number of abortion. Considering the mean volume of blood loss more than 3 pints of blood should be prepared befor delivery.

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7복강경하 난관복원수술 후 임신율에 관한 임상연구

저자 : 이위현(Wee Hyun Lee) , 차선희(Sun Hee Cha) , 이미화(Mee Hwa Lee)

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 42권 3호 발행 연도 : 1999 페이지 : pp. 487-490 (4 pages)

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Objective: Our purpose was to evaluate the pregnancy outcome and the advantages of laparoscopic tubal reanastomosis. Method; During 16 months, January 1996 to April 1997, thirty-two patient had underwent laparoscopic tubal reanastomosis in Pudang CHA General Hospital. The mean age of the patients was 36.1+-4.3 years(mean+-SD; range 26 to 47 years). Result: The intrauterine pregnancy rate of laparoscopic tubal reanastomosis was 72.4%(21/29). Data comparing laparoscopic procedure retrospectively to tubal reversal by laparotomy was also evaluated. The mean interval from operation to pregnancy was similar in the two groups (p=0.9). The operation time was sigoificantly longer for laparoscopy (215.3+-35.5 minutes) than for laparotomy(159.7+-52.3 minutes). Nevertheless, the intensity of postoperative pain was lower (p<0.05) in patient who underwent laparoscopy than in patient who underwent laparotomy. Also, the mean hospital stay (3.6+-2.3 days for laparoscopy, 6.1+-0.5 days for laparotomy) was shortened(p<0.05) after laparoscopy compared with laparotomy. Conclusion: Laparoscopic tubal reanastomosis may offer the benefits of lower postoperative pain and shorten recovery time in comparison with laparotomy. Therefore, considering the high pregnancy rate in minimal follow up period of 6 month, laparoscopic tubal reanastomosis could be an alternative procedure to microsurgical laparotomy in patients requesting reversal of sterilization.

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8TDx - FLM 에 의한 태아 폐성숙도 측정에 혈액 및 태변이 미치는 영향

저자 : 김수평(Soo Pyung Kim) , 신종철(Jong Chul Shin) , 이영(Young Lee) , 이종건(Jong Kun Lee) , 백은정(Eun Jeong Baik) , 김기홍(Ki Hong Kim) , 정대영(Dae Young Jung) , 김창이(Chang I Kim)

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 42권 3호 발행 연도 : 1999 페이지 : pp. 491-495 (5 pages)

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Objective: We studied to determine the effect of blood or meconium contamination on the TDx-FLM assay for the assessment of fetal lung maturity. We also studied to evaluate the degree of diluted contaminants that affect the results. Methods: Nineteen samples of amnotic fluid-14 cases < GA 37weeks and 5 cases > GA 37weeks-were collected and assayed for assessment of fetal lung maturity using tbe TDx-FLM assay. Among the above 19 samples, we used 12 samples-7 cases < GA 37weeks and 5 cases > GA37 weeks-to contaminate with blood or meconium. Maternal blood was added to the amniotic fluid at increasing concentrations fro 1:10 to 1:1280. Diluted meconium (0.5g meconium/10ml amniotic fluid) was added at increasing concentration fiom 1:1 to 1:128. Each samples were assessed by TDx assay. Results: TDx values in the cases of gestational age 37 weeks or more were matured level or borderline level(TDx value > 50mg/g), but below 37 weeks, TDx values wae immature level(TDx value < 50mg/g) except one case. In preterm cases, blood or meconium contamination did not affect the TDx values significantly, although the thick meamium contamination (diluted meconium: amniotic fluid 1:1 - 1:4) increased the TDx values. In term cases, they did not affect the TDx values. Conclusion: TDx test was suitable for the evaluation of fetal lung maturity regardless of blood or meconium contamination.

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9다낭성난소증후군 환자들에게서 배란유도시 성장억제호르몬 유사체가 난소반응에 미치는 영향

저자 : 추형식(Hyung Sik Chu) , 채희동(Hee Dong Chae) , 김정훈(Chung Hoon Kim) , 강병문(Byung Moon Kang) , 장윤석(Yoon Seok Chang) , 강은희(Eun Hee Kang)

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 42권 3호 발행 연도 : 1999 페이지 : pp. 496-503 (8 pages)

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Objective: To determine whether the somatostatin analogue, octreotide, given concunently with human menopausal gonadotropin (HMG) affects ovarian response, ovulation induction outcome, and serum levels of growth hormone (GH) and insulin-like growth factor 1 (IGF-1) in infertile patients with polycystic ovarian syndrome (PCOS) resistant to clomiphene citrate (CC). Methods: From August 1996 to June 1998, 42 infertile patients with PCOS urresponsive to CC were enrolled in the present study. Women who had other infertility factors were excluded from this study. Patients were randomly allocated either to combined HMG and octreotide treatment (treatment group) (n 21) or HMG alone (control group) (n=21). In the treatment group, 100 micrograms of octreotide were administered daily concomitantly with HMG from the 3rd day of menstrual cycle to the preceding day of human chorionic gonadotropin (HCG) injection. Results: Patient's characteristics were comparable in both groups. One cycle in the control group was abandoned because of excessive follicular development. However, none of the cycles in the treatment group was abandoned. There were no differences in the number of HMG ampules required and the duration of HMG administration between the two groups. The number of follicles > 14mm diameter on the day of HCG injection was significantly less in the treatment group than that in the control group (p<0.01). The serum estradiol level on the day of HCG injection was also significantly lower in the treatment group, with 1391.0 +- 695.5 pg/ml compared with 2217.5 +- 811.6pg/ml in the control group (p<0.001). The incidence of severe ovarian hyperstimulation syndrome seemed to be lower in the treatment group, but the difference did not achieve significance (4.9% vs 23.8%). There were no differences in the clinical pregnancy rate, miscarriage rate and multiple pregnancy rate between the two groups. Although serum GH levels were comparable between the two groups, serum IGF-1 level on the day of HCG injection was significantly higher in the treatment gmup than that in the control group (p<0.001). Conclusion: This study suggests that the combined octreotide and HMG treatment could improve hormonal milieu and folliculogenesis compared to HMG alone, and therefore may be effective in ovulation induction for patients with PCOS resistant to CC.

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10자궁경부암 세포에서 retinoid / interferon 에 의한 성장 억제 조절의 새로운 분자생물학적 기전

저자 : 김수평(Soo Pyung Kim) , 남궁성은(Sung Eun Namkoong) , 김승조(Seung Jo Kim) , 김은주(Eun Joo Kim) , 엄수종(Soo Jong Um) , 박종섭(Jong Sup Park) , 이근호(Keun Ho Lee) , 김찬주(Chan Joo Kim)

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 42권 3호 발행 연도 : 1999 페이지 : pp. 504-516 (13 pages)

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Objective: Retinoic acids (RAs) and interferons (IFNs) have been implicated in the growth regulation of cervical cancer cells, which was suggested by clinical trials and in vitro experiments. However, the molecular mechanisms of growth regulation are not fully defined, The purpose of this study is to assess the effect of RA and/or IFN on human cervical carcinoma cells in vitro and to analyze their action mechanisms in HPV-positive cervical carcinoma cells by molecular biologic studies. Methods: HPV-positive (CaSki, HeLa), HPV-negative (C33A, HT-3), and non-cervical cancer Cos-1 cell lines were treated with RA and/ar IFN. Their effects on cell growth were evaluated by the cell pmliferation assay and the following BrdU DNA incorporation assay. The molecular mechanism was further investigated by a series of immunoblottings and transient cotransfection assays, which were conducted in HeLa cells and C33A cells using the CAT reporter gene assay. To observe the down regulation of HPV E6/E7 gene expression by RA/IFN, reverse transcription-polymerase chain reaction (RT-PCR) was perforned. Results: The powth of RA-treated cells was less suppressed than that of IFN-treated cells. Combined treatment of RA and IFN leads to additive effect on the growth suppression of HeLa and CaSki cells. The proliferation activity was most severely reduced in Hela cells by treatment of both all-trans-RA (AtRA) and IFN-r. Combined treatment of AtRA/IFN-r causes a great increase in the level of interferon regulatory factor-1 (IRF-1) protein in HeLa cells, whereas no induction of IRF-1 was observed in C33A cells. The CAT gene expression for IRF-1 was greatly induced by IFN-r in HeLa cells. Immunoblotting assays shows the concurrent induction of p21 CDK inhibitor and dephosphorylation of Rb protein in HeLa cells. In RT-PCR, an individual treatment of either RA or IFN reduced HPV E6/E7 mRNA levels and significantly cooperative when both RA and IFN were treated. By deaeasing E6 levels, the p53 level was increased in HeLs cells treated with RA and/or IFN. Transient cotransfection of IRF-1 and p53 as the transcription factors leads to the cooperative activation of a common p21 promoter to regulate the cell cycle. Conclusion: RA/IFN suppressed the growth of HPV-positive cervical cancer cells. When they were both #treated, additive suppressive effects were observed in cellular proliferation as well as DNA synthesis. The growth suppressive effect is likely to be related to the increased expression of IRF-1 and p21 (antitumoral effect; p53-independent). The down regulation of HPV E6 gene suppression may account for the resultant increase of p53 levels (antiviral effect; p53-dependent). Both induced IRF-1 and p53 cooperatively augument tbe suppession of p21 CDK inhibitor, which results in dephosphorylation of pRb. Although clinical effects are likely complex and may include interactions of in vitro growth inhibitory effects with immunomodulatory and antiangiogeaetic effect, tbese results suggest the optimal clinical role for the combination of RA/IFN in the treatment of cervical canccers.

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