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대한천식알레르기학회> 천식 및 알레르기> 원저: Dermatophagoides pteronyssinus 항원 분획별 알레르기 면역반응

원저: Dermatophagoides pteronyssinus 항원 분획별 알레르기 면역반응

박중원 , 박상욱 , 고시환 , 김철우 , 이한일 , 오상환 , 홍천수
  • : 대한천식알레르기학회
  • : 천식 및 알레르기 17권2호
  • : 연속간행물
  • : 1997년 06월
  • : 151-164(14pages)
천식 및 알레르기

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As molecular size of the most major allergens of house dust mite(HDM) are known to be less than 30 kD, allergic immune reactions may be different according to the molecular fractions of HDM allergens. In this study, we fractionated D. pteronyssinus(D. pt) antigen according to its molecular weights,' fraction I(FI), II(FII, more than 30 kD), III(FIII, less than 30 kD), and evaluated the differences of allergic immune responses in 40 atopic patients. FIII(A/H ratio  2.44+-0.61) elicited stronger response on intradermal skin test than FI (0.76+0.10, p<0.001) and FII(0.88k0.12, p <0.001). The immunoblot analysis revealed that the nine out of 10 atopics had IgE binding bands with 15 kD protein of FIII which were not found in FI and FII. The geometric mean of absorben value of specific IgE to FIII was 0.067, which was higher than that of FII (0.013, p<0.001), with no difference from that of FI(0.042). The skin reactivity to whole  D. pt antigen correlated with FIII  specific IgE(r=0.3862, p<0.01), not with FI  (r=0. 2605, p=0.07) and FII  specific IgE(r=0. 1103, p=0.45). FI  specific IgG levels in atopic sera(n=40) were significantly higher than that of FII and FIII in all IgG subclasses (p<0.001). Both atopic(n=4) and nonatopic (n=4) groups showed multiple IgG, IgG1, and IgG2 binding ba,nds at high molecular proteins in immunoblot analysis. The prevalence and density of the IgG and IgG1 binding bands to 15 kD were higher and more intense in atopics than those of nonatopics. In conclusion, we suggest that fractionated antigens of D. pteronyssinus had different antigenicities and that the allergic irnmune responses to HDMs in atopics and nonatopic are different according to molecular fractions of HDM.

UCI(KEPA)

I410-ECN-0102-2009-510-005560762

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  • : 의약학분야  > 내과학
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  • : 계간
  • : 1226-8739
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  • : 학술지
  • : 연속간행물
  • : 1981-2012
  • : 1614


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32권4호(2012년 12월) 수록논문
최근 권호 논문
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1종설 : 장기 생존 형질세포

저자 : 이주석 ( Ju Suk Lee )

발행기관 : 대한천식알레르기학회 간행물 : 천식 및 알레르기 32권 4호 발행 연도 : 2012 페이지 : pp. 203-206 (4 pages)

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Plasma cells are usually considered short-lived plasma cells. This view is changing because plasma cells can survive for a long time in bone marrow. Long-lived plasma cells may be important in allergy because of the persistence of immunoglobulin E in allergic animal models after immune suppression. Allergic therapies efficiently control allergic symptoms but do not provide a cure, especially in adults. Elimination of long-lived plasma cells that secrete specific immunoglobulin E may provide a cure in allergic patients. The purpose of this review is to describe the creation of long-lived plasma cells and their importance in allergic diseases.

2종설 : 알레르기질환에서의 Type 2 Innate Lymphoid Cell의 역할

저자 : 모지훈 ( Ji Hun Mo )

발행기관 : 대한천식알레르기학회 간행물 : 천식 및 알레르기 32권 4호 발행 연도 : 2012 페이지 : pp. 207-212 (6 pages)

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Innate lymphoid cells have recently been identified to be a cell population that has morphology typical of lymphocytes but lacks rearranged antigen receptors. It can be classified into RORγt innate lymphoid cell, type 2 innate lymphoid cells and natual killer cells. Among them, type 2 innate lymphoid cells plays an important role in type 2 immunity by releasing type 2 cytokines, such as interleukin-5 and interleukin- 13, and makes a match for Th2 cell of the adaptive immune system. Type 2 innate lymphoid cells responses are initiated by interleukin-25 and interleukin-33, which are produced by several cell types, such as epithelial cells, tissue macrophages and dendritic cells. These cytokines strongly activate type 2 innate lymphoid cells, leading to the production of type 2 cytokines, including, interleukin-5 and interleukin- 13. In this review, we will discuss the characteristics and development of type 2 innate lymphoid cells and the role of type 2 innate lymphoid cells in allergic diseases in detail.

3논평 : TARC (CCL17), MDC (CCL22): 알레르기 염증반응의 생체 지표로서의 의의

저자 : 양현종

발행기관 : 대한천식알레르기학회 간행물 : 천식 및 알레르기 32권 4호 발행 연도 : 2012 페이지 : pp. 213-215 (3 pages)

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4소아 알레르기 환자에서 혈청 흉선과 활동화 조절 케모카인, 대식세포 유래 케모카인의 상승과 호산구 염증지표와의 연관성

저자 : 정보현 ( Bo Hyun Chung ) , 이해성 ( Hae Sung Lee ) , 서현석 ( Hyeon Seok Seo ) , 박하늘 ( Ha Neul Park ) , 지그프리드바우어 ( Siegfried Bauer ) , 서성철 ( Sung Chul Seo ) , 윤원석 ( Won Suck Yoon ) , 정지태 ( Ji Tae Choung ) , 유영 ( Young Yoo )

발행기관 : 대한천식알레르기학회 간행물 : 천식 및 알레르기 32권 4호 발행 연도 : 2012 페이지 : pp. 216-223 (8 pages)

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Background: Thymus and activation-regulated chemokine (CCL17) and macrophage-derived chemokine (CCL22) are known as important mediators in allergic inflammation. Recently many researchers have focused on these mediators, but the role of the serum CCL17 and CCL22 in allergic diseases in children are still controversial. The aims of this study were to compare serum levels of CCL17 and CCL22 between children with different manifestation of allergic diseases, such as bronchial asthma, allergic rhinitis and atopic dermatitis, to analyze relationship with blood eosinophil makers and to find clinical significance of these markers. Methods: A total of 49 children (14 bronchial asthma, 13 allergic rhinitis and 13 atopic dermatitis) and the 9 healthy control subjects were enrolled in this study. Clinical characteristics and serum chemokine (CCL17, CCL22) levels were analyzed. We examined whether serum levels of CCL17 and CCL22 would be related to serum immunoglobulin E levels, blood eosinophils and serum eosinophil cationic protein concentrations. Results: Serum levels of CCL17 and CCL22 were significantly higher in children with bronchial asthma and atopic dermatitis than that in controls. Serum levels of CCL17 and CCL22 were significantly related to serum immunoglobulin E levels, blood eosinophil counts and serum eosinophil cationic protein concentrations with different strength in children with allergic diseases. Conclusion: Serum CCL17 and CCL22 may play a crucial role in the chronic allergic inflammatory process and can be used as inflammatory markers. These suggest that serum CCL17 and CCL22 might be involved in the pathophysiology of allergic diseases in children.

5군대 훈련 중 흡입항원에 대한 감작률과 알레르기질환 유병률의 변화

저자 : 황세민 ( Se Min Hwang ) , 김희경 ( Hee Kyung Kim ) , 김성화 ( Seoung Hwa Kim ) , 강점숙 ( Jum Sook Kang ) , 주영훈 ( Young Hoon Joo ) , 이상민 ( Sang Min Lee )

발행기관 : 대한천식알레르기학회 간행물 : 천식 및 알레르기 32권 4호 발행 연도 : 2012 페이지 : pp. 224-230 (7 pages)

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Background: The objective of this study was to evaluate changes in the prevalence of sensitization to inhalant allergens and allergic disease among young army conscripts during a 35-day military training course. Methods: Fifty-four young soldiers who conscripted into the Korean army on April 29, 2011 were enrolled after their informed consent. Their sera were sampled on day 1 to measure total immunoglobulin E and allergen-specific immunoglobulin E to common inhalant allergens using UNICAP(R) and the Multiple Allergen Simultaneous Test(R), respectively. Their sera were sampled again on day 35 to evaluate temporal changes in total immunoglobulin E and allergen-specific immunoglobulin E. Subjects were also requested to respond to the modified International Study of Asthma and Allergies in Childhood questionnaire on day 35 to evaluate the prevalence of allergic diseases. Results: The number of subjects sensitized to birch/alder increased from 1 (1.9%) to 4 (7.4%) during military training; however, the difference was not statistically significant. The status of sensitization to other allergens and serum total immunoglobulin E levels did not change significantly. Of the 54 subjects, 9 (16.7%) experienced new onset or exacerbation of allergic disease. However, most of subjects were not diagnosed with allergic diseases by a physician, nor did they receive proper treatment for their symptoms. Conclusion: A 35-day course of military training was insufficient to change the inhalant allergen sensitization status; however, some young army conscripts suffered from new onset or exacerbation of allergic diseases during military training that were not diagnosed or treated appropriately.

6MLE12 및 RAW264.7세포에서 Alternaria와 Alternariol Mycotoxin의 알레르기 염증반응 효과

저자 : 최길순 ( Gil Soon Choi ) , 김희규 ( Hee Kyoo Kim )

발행기관 : 대한천식알레르기학회 간행물 : 천식 및 알레르기 32권 4호 발행 연도 : 2012 페이지 : pp. 231-238 (8 pages)

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Background: Alternaria and their secondary metabolites, alternariol mycotoxins, may cause respiratory symptoms and asthma. Moreover, microbial compounds, such as bacterial endotoxins, act as potential contributors in such situations. Alveolar epithelial cells and macrophages appear to initiate airway inflammation through proinflammatory cytokines after exposure to aeroallergens or toxic materials. The present study investigated the effects of Alternaria and alternariol mycotoxins on inflammatory cytokines secreted from airway epithelial cells and macrophages, especially interleukin-33, interleukin-6 and tumor necrosis factor-α, which were assessed association with lipopolysaccharide. Methods: In vitro MLE12 cells and RAW264.7 cells were treated with Alternaria, alternariol monomethyl ether and alternariol at different doses for 24 hour. The levels of interleukin- 33, interleukin-6, and tumor necrosis factor-α in each cell supernatants were measured by ELISA. Then, each mycotoxin (100 ng/mL) was added to lipopolysaccharide stimulated cells and incubated with them. Cell viability was measured by flow cytometry. Results: Alternaria and alternariol mycotoxin-treated cells released pro-inflammatory cytokines partly in a dose-dependent manner. Moreover, lipopolysaccharide induced the synergic effect of mycotoxins on cytokine production by RAW264.7 cells. However, the different cytokine productions among groups were not related to cell viability. Conclusion: This study suggests that alternariol mycotoxins, such as Alternaria, would provoke airway inflammation through interleukin-33, interleukin-6 and tumor necrosis factor- α uction from epithelial cells and macrophages.

7만성기침에 대한 한국 알레르기 전문의들의 인식과 진료 실태; 설문조사

저자 : 김상헌 ( Sang Heon Kim ) , 김세훈 ( Sae Hoon Kim ) , 송우정 ( Woo Jung Song ) , 권재우 ( Jae Woo Kwon ) , 김민혜 ( Min Hye Kim ) , 김경묵 ( Kyung Mook Kim ) , 정재원 ( Jae Won Jeong ) , 김상훈 ( Sang Hoon Kim ) , 안영민 ( Young Min Ahn ) , 조은정 ( Eun Jung Jo ) , 이승은 ( Seung Eun Lee

발행기관 : 대한천식알레르기학회 간행물 : 천식 및 알레르기 32권 4호 발행 연도 : 2012 페이지 : pp. 239-253 (15 pages)

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Background: Chronic cough is a common but disabling respiratory symptom. While patients are frequently referred to allergists for the management of chronic cough, little is known about the perception and clinical practices of allergists on the management of chronic cough. Methods: We performed a questionnaire survey using e-mail and internet platform among the members of the Korean Academy of Asthma, Allergy and Clinical Immunology (n=823). The members were asked to answer the questions regarding the diagnosis and treatment of chronic cough. Results: Questionnaires were completed and returned from 154 subjects (18.7%). There was a heterogeneity in the definitions (durations) of chronic cough, and only 62 subjects (40.3%) defined chronic cough when cough lasted more than 8 weeks. Most subjects (n=131, 85.1%) felt the need for Korean guidelines on chronic cough, while only 89 subjects (57.8%) used the other guidelines in their practice. Various diagnostic tests were used for the evaluations of chronic cough, and chest radiography, paranasal sinus radiography and spirometry were the most frequently performed for the management. Upper airway cough syndrome was perceived as the most common cause of chronic cough, followed by asthma, postinfectious cough and gastroesophageal reflux disease/laryngopharyngeal reflux. Moreover, treatment patterns of these most common clinical conditions differ among studied subjects. Conclusion: There were diverse patterns in the diagnosis and treatment of chronic cough among Korean allergists. Our results could assist in establishing guidelines and health policy on chronic cough management.

8만성 자발성 두드러기 환자에서 자가혈청 피부반응검사에 따른 검사실 소견과 증상 조절을 위한 약제의 비교

저자 : 이수경 ( Su Kyoung Lee ) , 조민호 ( Min Ho Cho ) , 김병연 ( Byeong Yeon Kim ) , 강윤성 ( Yun Sung Kang ) , 최승헌 ( Seung Heon Choi ) , 손성욱 ( Seong Wook Sohn )

발행기관 : 대한천식알레르기학회 간행물 : 천식 및 알레르기 32권 4호 발행 연도 : 2012 페이지 : pp. 254-258 (5 pages)

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Background: The autologous serum skin test is reported to be positive in up to 60% of patients with chronic spontaneous urticaria. However, the clinical role of autologous serum skin test is still poorly understood. Methods: We reviewed the medical records of 166 chronic spontaneous urticaria patients whose symptoms lasted for more than 6 weeks and compared their laboratory findings and medication levels according to autologous serum skin test reactivity. Results: Autologous serum skin test was positive in 69 (41.6%) chronic spontaneous urticaria patients. There were no significant differences between autologous serum skin test-positive and autologous serum skin test-negative groups in laboratory findings, such as anti-microsome antibody, anti- thyroglobulin antibody, anti-nuclear antibody, total immunoglobulin E, complement 4 level levels, rheumatoid factor, erythrocyte sedimentation rate and D-dimer. Autologous serum skin test were not related to medication levels. Eight patients (11.6%) of autologous serum skin test-positive groups and 6 patients (6.2%) of autologous serum skin test-negative groups need level 4 medication (usage of cyclosporine, dapsone or anti-immunoglobulin E monoclonal antibody) to control urticaria without statistical significance. Conclusion: Autologous serum skin test was positive in more than 40% of chronic spontaneous urticaria patients. However, laboratory findings and medication levels were not affected by autologous serum skin test reactivity.

9증례 : 수은 증기 흡입에 의한 급성 폐손상 및 급성 전신성 발진성 농포증 1예

저자 : 서현정 ( Hyun Jung Seo ) , 명유식 ( Yu Sik Myung ) , 정희재 ( Hee Jae Jung ) , 김민진 ( Min Jin Kim ) , 서유리 ( Yu Ri Seo ) , 박경아 ( Kyong Ah Park ) , 박춘식 ( Choon Sik Park ) , 박재성 ( Jai Soung Park ) , 박종숙 ( Jong Sook Park )

발행기관 : 대한천식알레르기학회 간행물 : 천식 및 알레르기 32권 4호 발행 연도 : 2012 페이지 : pp. 259-263 (5 pages)

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Background: Inhalation of mercury vapor is very rare, but if it occurs, fatal damage, such as acute lung injury or death, may ensure. In addition, acute generalized exanthematous pustulosis due to mercury hypersensitivity has rarely been reported in the literature. We experienced a 49-year-old male patient who had been exposed to mercury vapor following burning of mercury-containing materials. Case History: The patient complained of dyspnea, fever, and systemic erythema. Physical examination revealed that he had macular erythema with tiny pustules. His body temperature was 38.3oC and a complete blood count revealed elevated leucocytes. Chest radiographs revealed diffuse airspace opacification in both lungs. Results: He was admitted to the intensive care unit and treated with oxygen, intravenous methylprednisolone 125 mg per day, and oral antihistamines. His symptoms improved and his skin lesions resolved completely with desquamation 5 days. On the eighth hospital day, intravenous methylprednisolve was tapered to 62.5 mg methyprednisolone, followed by oral methyprednisolone. He was discharged from the hospital on the 20th hospital day and is currently being followed-up with no aggravation of symptoms or relapse. Conclusion: We report a case of acute lung injury and acute generalized exanthematous pustulosis induced by inhalation of mercury vapor that was successfully treated with early avoidance and steroid therapy.

10증례 : 유전성 혈관부종 환자에서의 성공적인 제왕절개 분만 1예

저자 : 이서영 ( Suh Young Lee ) , 이승은 ( Seung Eun Lee ) , 김민혜 ( Min Hye Kim ) , 송우정 ( Woo Jung Song ) , 강혜련 ( Hye Ryun Kang ) , 민경업 ( Kyung Up Min )

발행기관 : 대한천식알레르기학회 간행물 : 천식 및 알레르기 32권 4호 발행 연도 : 2012 페이지 : pp. 264-267 (4 pages)

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Background: Hereditary angioedema is a rare disease caused by a deficiency of C1 inhibitor and inherited in an autosomal dominant manner. It leads to episodic abdominal pain and angioedema which can be life-threatening. Associations between hereditary angioedema and pregnancy have been reported in the literature. The frequency of symptoms increases during pregnancy and the postpartum period. While complications during vaginal delivery are generally known to be rare in patients with heterditary angioedema, acute attack of heterditary angioedema may occur during Cesarean section. Case History: A 34-year-old pregnant woman was admitted for the induction of labor with suspected preeclampsia. The patient was diagnosed with hereditary angioedema 5 years ago and treated with tranexamic acid when needed. During the first trimester of pregnancy, the incidence of attacks increased but she did not take any medication. She had prophylactic tranexamic acid for 2 weeks before delivery. Emergency Cesarean section was performed due to fetal deceleration that occurred during labor induction. Results: After injection of a C1 inhibitor in the phase of anesthesia induction, delivery was successful without any complications. Conclusion: We report a case of Cesarean section delivery with a prophylactic use of a C1 inhibitor in a patient with hereditary angioedema.

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1종설: Cytokine 전사인자

저자 : 최지호

발행기관 : 대한천식알레르기학회 간행물 : 천식 및 알레르기 17권 2호 발행 연도 : 1997 페이지 : pp. 99-107 (9 pages)

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2종설: 유도 객담검사의 진단적 의의

저자 : 홍천수

발행기관 : 대한천식알레르기학회 간행물 : 천식 및 알레르기 17권 2호 발행 연도 : 1997 페이지 : pp. 108-114 (7 pages)

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3논평: 아토피성 피부염과 알레르겐

저자 : 편복양

발행기관 : 대한천식알레르기학회 간행물 : 천식 및 알레르기 17권 2호 발행 연도 : 1997 페이지 : pp. 115-118 (4 pages)

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4원저: U937세포주의 분화에 따른 lipocortin 1의 발현 및 glucocorticoid의 영향

저자 : 조영주 , 문희범 , 나도선

발행기관 : 대한천식알레르기학회 간행물 : 천식 및 알레르기 17권 2호 발행 연도 : 1997 페이지 : pp. 119-128 (10 pages)

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Lipocortin represents a family of similar Ca" depentent phospholipid  binding proteins capable of blocking the activity of phospholipase A (PLA) in vitro. Generally, these proteins are believed to inhibit the release of arachidonic acid from phos- pholipids and the formation of lipid mediators such ss prostaglandin, leukotriene, and platelet activating factor. Lipocortin 1, initially identified as a glucocorticoid  responsive protein in macrophages and neutrophils has been implicated in transmembrane signal transduction during growth factor  mediated cell proliferation and transformation. To define the synthesis and its regulation, we investigated the expression of lipocortin 1 in both the mRNA and protein level in U937 cell line in the presence of several differentiation factors. The results were as follows. 1. The expression of lipocortin 1 and its mRNA was increased during TPA  induced differentiation of U937 cells to maximum of 2 -fold and 5-fold respectively. Both the protein  and mRNA levels decreased after 48 hours. 2. With the treatment with IFN  y, the ex- pression of CD16 was increased. However, the protein and mRNA levels of lipocortin 1 were, not changed significantly. 3. Neither the dexamethasone or hydrocortisone have any effects on the expression of lipocortin 1 in both TPA-differentiated and undifferentiated U937 cells. The results from this study would give a light on defining the functional role of lipocortin 1 in macro  monocyte cell lineage and possibly some informstive clues for the pathogenic mechanisms of the inflammatory diseases.

5원저: 혈소판활성인자(PAF) 흡입에 의한 기도과민성 : 천식의 중증도에 따른 차이

저자 : 김미경 , 김유영

발행기관 : 대한천식알레르기학회 간행물 : 천식 및 알레르기 17권 2호 발행 연도 : 1997 페이지 : pp. 129-140 (12 pages)

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PAF itself induces the non-specific bronchial hyperreactivity(NSBH) in asthma. NSBH induced by PAF is nearly as same as that by the sensitized allergen inhalation even though PAF never provokes the late asthmatic response. Usually the amount of allergen for BPT inversely correlated with the severity of asthma, but in PAF, nothing was clarified about that, yet. Usually eosinophil is the main effector cell in PAF-induced NSBH. So we want to declare whether PAF inhalation affects the NSBH differently according to the severity of asthma or not in this study and eosinophil is the main effector cell in asthmatics. Single dose PAF(400ug/ml) inhalation challenge was done by the Cockcrofts method but that of methacholine by Chais method before and after PAF until NSBH returned the basal value. From that, we calculated PC20 Also we checked the eosinophil and  platelet count in peripheral blood before and after PAF until those returned the baseline. All these were done in two groups, mild asthmatics and moderate asthmatics classified by the PFT and their PC20 The Bronchoconstriction started at the 3 minutes after PAF inhalation and then relieved completely 30 minutes after inhalation but in mild asthmatics bronchi constricted much more than moderate asthmatics. The fall of NSBH also persisted much longer in mild asthmatics. But the decrease in peripheral eosinophil counts was nearly same in both groups. None of asthmatics showed the chang- es in platelet counts in peripheral blood. The degree of bronchoconstriction and decrease in eosinophil counts didnt have any correlation with the persistence of the NSBH. Taken together, PAF inhalation affected the NSBH inversely according to the severity of asthma in this study but eosinophil might not be the only effector cell in PAF-induced NSBH.

6원저: 집먼지진드기에 감작된 천식 환자에서의 항원에 의한 말초혈액 호산구 활성화

저자 : 김미경

발행기관 : 대한천식알레르기학회 간행물 : 천식 및 알레르기 17권 2호 발행 연도 : 1997 페이지 : pp. 141-150 (10 pages)

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In asthma, eosinophil roles as main end inflammatory effector cell, but neither the activation  process nor the stimuli of that is known dearly, yet. Even though allergic asthma is IgE  medisted type I hypersensitivity disease, it is not clear whether there is FceRI on the surface of eosino phil like mast cell or basophil which is activated through the FceRI bridging with sensitized allergens. In this study, we speculate if theres FceRI on the eosinophil, sensitized allergen activates it like mast cell in allergic asthmatics, and with this result we can find out one of the stimuli of eosinophil in asthma and can suggest the possibility of the existence of FceRI on the eosinophil. We did direct stimulation of the house dust mite  sensitized asthmatics purified peripheral blood normodense eoisnophils with Dermatophagoides pteronyssinus. Then we measured the LTC, and ECP from the supernatant by RIA kit and observed the morphological changes with electron microscopy. There was neither increase in LTC< and ECP level nor the morphological changes in asthmatics. And there were no significant differences in both LTC<, and ECP level, and morphologic changes between asthmatics and controls. We would summerize that house dust mite dose not dirctly activate eosinophil from house dust mite  sensitized asthmatics and couldnt find out the possibility that theres FceRI on the surfsce of eosinophils. But no existence of FceRI on the eosinophil is the indirect evidence, so from now on we try to find more direct evidence of that by blotting.

7원저: Dermatophagoides pteronyssinus 항원 분획별 알레르기 면역반응

저자 : 박중원 , 박상욱 , 고시환 , 김철우 , 이한일 , 오상환 , 홍천수

발행기관 : 대한천식알레르기학회 간행물 : 천식 및 알레르기 17권 2호 발행 연도 : 1997 페이지 : pp. 151-164 (14 pages)

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As molecular size of the most major allergens of house dust mite(HDM) are known to be less than 30 kD, allergic immune reactions may be different according to the molecular fractions of HDM allergens. In this study, we fractionated D. pteronyssinus(D. pt) antigen according to its molecular weights,' fraction I(FI), II(FII, more than 30 kD), III(FIII, less than 30 kD), and evaluated the differences of allergic immune responses in 40 atopic patients. FIII(A/H ratio  2.44+-0.61) elicited stronger response on intradermal skin test than FI (0.76+0.10, p<0.001) and FII(0.88k0.12, p <0.001). The immunoblot analysis revealed that the nine out of 10 atopics had IgE binding bands with 15 kD protein of FIII which were not found in FI and FII. The geometric mean of absorben value of specific IgE to FIII was 0.067, which was higher than that of FII (0.013, p<0.001), with no difference from that of FI(0.042). The skin reactivity to whole  D. pt antigen correlated with FIII  specific IgE(r=0.3862, p<0.01), not with FI  (r=0. 2605, p=0.07) and FII  specific IgE(r=0. 1103, p=0.45). FI  specific IgG levels in atopic sera(n=40) were significantly higher than that of FII and FIII in all IgG subclasses (p<0.001). Both atopic(n=4) and nonatopic (n=4) groups showed multiple IgG, IgG1, and IgG2 binding ba,nds at high molecular proteins in immunoblot analysis. The prevalence and density of the IgG and IgG1 binding bands to 15 kD were higher and more intense in atopics than those of nonatopics. In conclusion, we suggest that fractionated antigens of D. pteronyssinus had different antigenicities and that the allergic irnmune responses to HDMs in atopics and nonatopic are different according to molecular fractions of HDM.

8원저: 소아 아토피성 피부염의 식품 , 집먼지진드기 항원 연관성

저자 : 김정희 , 정세우 , 임대현 , 손병관 , 손진아 , 이상일 , 차광은

발행기관 : 대한천식알레르기학회 간행물 : 천식 및 알레르기 17권 2호 발행 연도 : 1997 페이지 : pp. 165-170 (6 pages)

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Although basic mechanisms of atopic dermatitis remain largely speculative, many studies on pathogenesis suggest the importance of food and inhalent allergens. To evaluate the frequency of food and house dust mite hypersensitivity and differences in this frequency according to ages, we measured the levels of specific IgE antibodies to egg white, egg yolk, milk, soy, and house dust mites in 119 children with atopic dermatitis. The results showed that 53% of patients had positive RAST to any one kind of allergens. The frequency of food and house dust mite hypersensitivity were 34.5%, 30.3 %, respectively. Among allergens, house dust mites and egg white are the most prevalent allergens in all atopic dermatitis patients. The Prevalence of egg white is most common under the age of 2 years, but those of house  dust mites are the highest in the ages of 5  12 years. In conclusion, we recommend an egg restriction diet in atopic dermatitis patients who are less than 2 years old when their symptoms do not improve with general skin care.

9원저: 아스피린 - 과민성 천식 : Lysine - aspirin 기관지유발시험의 진단적 유용성

저자 : 박해심 , 김윤정 , 김희연 , 남동호 , 윤윤보

발행기관 : 대한천식알레르기학회 간행물 : 천식 및 알레르기 17권 2호 발행 연도 : 1997 페이지 : pp. 171-179 (9 pages)

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Aspirin(ASA) and NSAIDs can induce bronchoconstriction in 10 20% of adult asthmatics patients. Inhalation of lysine  ASA(L  ASA) has been described as an alternative method for diagnosis of ASA  sensitive asthma. To further understand the characteristics of ASA  sensitive asthma, we studied 38 asthmatic patients with ASA  sensitivity (36 intrinsic and 2 extrinsic asthma) proven by L  ASA bronchoprovocation test (BPT). Most were female (male to female ratio was 27:73). Twenty (53%) of them had no previous history of adverse reactions when exposed to ASA. Twenty nine (79% ) had rhino  sinusitis symptoms. Early asthmatic response was observed in 16 (42% ) patients, late only response in 16(42%), and dual response in 6 (16% ) patients. The threshold of L  ASA to provoke a positive response ranged from 11.2 to 180 mg/ml and most (68.3% ) had a positive response after the inhalation of 180 mg/ ml. Concurrent sensitivity to sulfite was noted in 14 (36%) patients, followed by sensitivity to tartrazine in one (3% ) patient. None showed a positive response to sodium benzoate. After the avoidance from ASA / NSAIDs with administration of anti  asthmatic medications, symptom and medication scores re- duced in 26(87%) patients among 30 followed patients. They were classified into the improved group'. Four (13%) patients belonged to the not  improved group. There were no significant differences in clinical characteristics between the improved and not  improved group (p>0.05). In conclusion, L  ASA BPT could be considered as a useful method to diagnose ASA  sensitive asthma and be used to screen the causative agent for asthmatic patients with intrinsic type, especially in female patients with rhino  sinusitis and/or nasal polyp, even though they do not have any history of adverse reactions. Cessation of exposure and proper treatment may allow to reduce symptom and medication scores.

10원저: 만성 기침 환자에서의 객담내 호산구 측정의 의의

저자 : 오영배 , 문찬희 , 김희연 , 이상무

발행기관 : 대한천식알레르기학회 간행물 : 천식 및 알레르기 17권 2호 발행 연도 : 1997 페이지 : pp. 180-185 (6 pages)

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We studied sputum eosinophil count of patients with chronic cough. Differential count of sputum cells(simple direct smear with Wright stain) were successfully examined in 44(73%) among 60 patients with chronic cough. The patients were divided according to PC20 value, 20(45% ) patients had increased airway hyper responsiveness(AHR)(PC20 <  24mg/mL), remained 24 patients did not have any evidence of airway AHR. Age, sex, duration and severity of symptoms, serum total IgE and positive skin test reaction were not different between two groups. In patients with increased AHR, increased sputum eosinophil ratio(10%) was found in 11 patients (55 % ) and increased peripheral blood eosinophil ratio(>5%) was found in 66% of patients with increased AHR. In patients without increased AHR, increased sputum eosinophil ratio was found in three patients(12% ) and increased peripheral blood eosinophil ratio was found in 24%. This discrepancy between two groups was statistically significant(p<0.01), In regarding to sputum eosinophilia, increased sputum eosinophil ratio was in 14(32 %) of 44 patients with chronic cough. Eleven patients(78% ) of them had increased AHR. Only nine(30% ) among 30 pstients without sputum eosinophilia had increased AHR. These discrepancies between two groups were statistically significant(p<0. 01). To predict AHR, sputum eosinophilia has 55% of sensitivity and 88% of specificity. Significant positive correlation between peripheral blood eosinophil ratio and sputum eosinophil ratio was found in pstients with cough  variant asthma (r=0.76, p<0.01). We suggest that simple direct smear for sputum eosinophil count could be an useful test to diagnose the patients with chronic cough.

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