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Plant breeding and biotechnology update

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수록정보
수록범위 : 1권1호(2013)~10권3호(2022) |수록논문 수 : 369
Plant breeding and biotechnology
10권3호(2022년 09월) 수록논문
최근 권호 논문
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KCI등재 SCOPUS

저자 : Tae-lim Kim , Kyungmi Lee , Hwan-su Hwang , Changyoung Oh , Il Hwan Lee , Hyemin Lim

발행기관 : 한국육종학회 간행물 : Plant breeding and biotechnology 10권 3호 발행 연도 : 2022 페이지 : pp. 145-162 (18 pages)

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Drought stress is a crucial environmental factor for plant survival, and the development of drought-tolerant varieties is one of the goals of all cultivated plant researchers. In particular, the seedling stage is important for plant growth and development and is also the period most affected by drought. We investigated the initial response to drought stress in seedlings of two species of poplar (Populus alba × Populus davidiana and Populus davidiana) that phenotypically differed in drought tolerance. Comparative analysis in terms of shoot height, photosynthetic pigments, soluble sugars, antioxidants, proline, soluble protein, malondialdehyde (MDA), and hydrogen peroxide (H2O2) contents was used to measure the physiological and biochemical characteristics of drought stress, and drought-related genetic changes were also examined. Significant changes in shoot height, chlorophyll fluorescence, chlorophyll contents, MDA and H2O2 appeared more adversely in Populus alba × Populus davidiana than in Populus davidiana, whereas reductions in soluble protein, carotenoids, superoxide dismutase (SOD) and catalase (CAT), which are indicators related to drought tolerance, appeared less in Populus davidiana. The change pattern of genes related to electron transfer and H2O2 production were almost similar in the two species, and among the drought response genes, lipid transfer protein 3 (LTP3) was greatly upregulated only in Populus davidiana. In the initial response to drought stress of both poplars, Populus davidiana, which had good antioxidant maintenance, showed better drought tolerance than Populus alba × Populus davidiana, which had a faster response to osmotic balance control.

KCI등재 SCOPUS

저자 : Jong-geun Park , Jeong-eui Hong , Md Abdur Rahim , Ill-sup Nou

발행기관 : 한국육종학회 간행물 : Plant breeding and biotechnology 10권 3호 발행 연도 : 2022 페이지 : pp. 163-173 (11 pages)

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Squash (Cucurbita moschata D.) is an economically important vegetable of the Cucurbitaceae family. The genetic purity of commercial hybrid seed is crucial for the success of hybrid seed production. The molecular markers like single nucleotide polymorphism (SNP) can efficiently and cost-effectively distinguish the genetic differences among F1 hybrid cultivars. Therefore, in this study, we used 'Fluidigm SNP Genotyping' assay using 27 SNPs to distinguish and purity analysis of registered commercial F1 hybrid cultivars and F1 breeding lines of squash. Of these, eight SNP markers, including CMo-A01, CMo-A02, CMo-A04, CMo-A05, CMo-A12, CMo-A16, CMo-A20 and CMo-A25 can successfully identified heterozygotes from the registered commercial F1 hybrid squash cultivars with 100% accuracy and partial contamination was detected for F1 hybrid squash breeding lines which resulted due to outcrossing. Moreover, the HRM analysis of a registered commercial F1 hybrid cultivar 'Parangsae' with CMo-A03 SNP marker showed 96.30-100% purity of the cultivar. Our results suggest that the 'Fluidigm SNP Genotyping' technology could be a rapid and cost-effective method for cultivar differentiation and genetic purity analysis of F1 hybrids and squash cultivars.

KCI등재 SCOPUS

저자 : Woojong Jang , Yeeun Jang , Woohyeon Cho , Sae Hyun Lee , Hyeonah Shim , Jee Young Park , Jiang Xu , Xiaofeng Shen , Baosheng Liao , Ick-hyun Jo , Young Chang Kim , Tae-jin Yang

발행기관 : 한국육종학회 간행물 : Plant breeding and biotechnology 10권 3호 발행 연도 : 2022 페이지 : pp. 174-185 (12 pages)

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Cultivation of the medicinal herb Panax ginseng Meyer began by domesticating wild mountain ginsengs several hundred years ago in Korea. Elucidating the diversity of the maternally inherited plastid genome (plastome) in diverse ginseng collections including wild ginsengs would provide valuable information on ginseng breeding and cultivation history. We sequenced and compared the plastomes of 44 ginseng accessions collected from various Northeast Asian countries. The plastomes revealed 18 polymorphic sites, including 11 SNPs and 7 InDels, which portrayed less diversity than in the most closely related species, P. quinquefolius. We developed 10 kompetitive allele-specific PCR (KASP) markers and utilized them along with four previously developed InDel markers to characterize the genotypes of 203 ginseng accessions. Digital genotyping based on the developed KASP markers classified the accessions into 10 main and 2 branching haplotypes. Four InDel markers derived from different copy numbers of tandem repeats showed dynamic subgrouping within the haplotypes due to the occurrence of multi-alleles and reversible mutations. The digital haplotype genotyping (haplotyping) revealed that haplotype A, representing 60.1% of the accessions, might be the original plastome form without any SNP occurrence. Accumulation patterns of the variations suggest that nine main haplotypes (B-J) diverged independently by new SNP occurrences from the original plastome, and branching haplotypes may have derived from the first mutant lineage by additional SNP deposition. The digital haplotyping system based on plastome diversity deepens understanding of ginseng evolution and serves as a useful molecular breeding tool.

KCI등재 SCOPUS

저자 : Jelli Venkatesh , Seo-young Lee , Hwa-jeong Kang , Seyoung Lee , Joung-ho Lee , Byoung-cheorl Kang

발행기관 : 한국육종학회 간행물 : Plant breeding and biotechnology 10권 3호 발행 연도 : 2022 페이지 : pp. 186-196 (11 pages)

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Targeted genome editing using CRISPR/Cas nucleases has become the standard approach for creating mutant plants. Significant progress has been made to enhance the editing efficiencies through optimizing CRISPR/Cas expression, including applying heat stress. In this study, we used heat stress to enhance the Potato virus X (PVX)-mediated CRISPR/Cas9 mutagenesis in Nicotiana benthamiana. We show that heat stress at 4-5 days after PVX inoculation effectively increases the mutagenesis efficiency of Cas9 nuclease. We observed up to a 5-8% increase in mutation efficiency depending on the sgRNA construct when heat stress is applied to the pPVX-Cas9::sgRNA infiltrated samples. Furthermore, analysis of the effect of the heat stress on the pattern of mutation types in the target gene regions showed no obvious changes in CRISPR/Cas9 induced mutagenesis pattern between heat stress treated and no heat stress treated samples. Overall, our experiments demonstrate that heat stress treatment at the optimal time after viral inoculation is most effective in increasing the PVX-mediated CRISPR/Cas9 editing efficiency in plants.

KCI등재 SCOPUS

저자 : Hyun Jo , Ammala Namsavanh , Changwan Woo , Hwayeop Kim , Syada Nizer Sultana , Jong Tae Song , Jeong-dong Lee

발행기관 : 한국육종학회 간행물 : Plant breeding and biotechnology 10권 3호 발행 연도 : 2022 페이지 : pp. 197-202 (6 pages)

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The number of four-seeded pods is a plant trait that is of great interest in terms of increasing soybean production. The objective of this study was to understand the agronomic characteristics of four-seeded pods of FS1159, which contain a significantly higher ratio of four-seeded pods than do other genotypes. FS1159 showed a significantly lower ratio of one- and two-seeded pods and a significantly higher ratio of three- (39.6%) and four- (11.3%) seeded pods than did the four check soybeans. The average values of the traits of FS1159 in this study were: plant height, 58.1 cm; the number of nodes, 15.7; the number of branches, 6.5; and 100-seed weight, 20.3 g. These results indicate that FS1159 can be used as a new genetic resource to explore the traits of four-seeded-pod and improve the soybean yield.

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