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The Korean Journal of Mycology

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수록정보
수록범위 : 1권1호(1973)~49권4호(2021) |수록논문 수 : 2,225
한국균학회지
49권4호(2021년 12월) 수록논문
최근 권호 논문
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KCI등재 SCOPUS

1Acaulospora jejuensis, a New Species in Glomeromycota from Korea

저자 : Hyeok Park , Kang-hyeon Ka , Ahn-heum Eom

발행기관 : 한국균학회 간행물 : 한국균학회지 49권 4호 발행 연도 : 2021 페이지 : pp. 425-431 (7 pages)

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New species of arbuscular mycorrhizal fungi (Glomeromycota), Acaulospora jejuensis, was isolated from rhizosphere soils of Miscanthus sinensis in the grassland in Jeju Island of Korea. The species was identified using the morphological characteristics of the spores and the molecular phylogenetic analysis using partial DNA sequences from small subunit rDNA (SSU), internal transcribed spacer (ITS), and large subunit rDNA (LSU). Phylogenetic analysis placed the fungal species in a distinct clade within genus Acaulospora. Also, the species exhibited the morphological characteristics distinct from the other members of the genus. Therefore, Acaulospora jejuensis was described as a novel species from Korea.

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2Antioxidant Activity of Indigenous Trametes Species in Korea

저자 : Minkyeong Kim , Chorong Ahn , Changmu Kim

발행기관 : 한국균학회 간행물 : 한국균학회지 49권 4호 발행 연도 : 2021 페이지 : pp. 433-440 (8 pages)

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The fungal genus Trametes is globally distributed and comprises various wood-decay species, including the well-known medicinal mushroom Trametes versicolor , a popular remedy in traditional Asian medicine. Trametes species produce antioxidants, which have a wide range of health benefits. The pressent study evaluated seven indigenous Trametes species from Korea, which were cultivated in three different media (dextrose-yeast extract, DY; malt extract-yeast extract, MY; malt extract broth, MEB) and tested for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays. We found that the medium consumption rate did not significantly differ between the media and among the strains (72-76%). However, the T. versicolor strains had a relatively low consumption rate (14-65%). The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3- ethylbenzothiazoline-6-sulfonate) (ABTS) tests demonstrated that culture filtrates of T. cf. junipericola, T. orientalis, T. suaveolens, and T. versicolor possessed antioxidant activity against damage from free radicals. In particular, T. cf. junipericola (DY) and T. versicolor (MY) had >80% activity in the DPPH and ABTS assays, compared with that of the positive control (ascorbic acid). Thus, our study identified promising candidates with substantial antioxidant activity among the indigenous strains of Trametes spp. from Korea.

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3Isolation and Characterization of an Antifungal and Plant Growth-Promoting Microbe

저자 : Se Won Park , Hee-jong Yang , Ji Won Seo , Jinwon Kim , Su-ji Jeong , Gwangsu Ha , Myeong Seon Ryu , Hee Gun Yang , Do-youn Jeong , Hyang Burm Lee

발행기관 : 한국균학회 간행물 : 한국균학회지 49권 4호 발행 연도 : 2021 페이지 : pp. 441-454 (14 pages)

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Fungal diseases including anthracnose, stem rot, blight, wilting, and root rot of crops are caused by phytopathogens such as Colletotrichum species, Sclerotinia sclerotiorum, Phytophthora species, and Fusarium oxysporum and F. solani which threaten the production of chili pepper. In this study, to identify biological control agents (BCAs) of phytopathogenic fungi, potentially useful Bacillus species were isolated from the field soils. We screened out five Bacillus strains with antagonistic capacity that are efficiently inhibiting the growth of phytopathogenic fungi. Bacillus species were characterized by the production of extracellular enzymes, siderophores, and indole-3-acetic acid (IAA). Furthermore, the influence of bacterial strains on the plant growth promoting activity and seedling vigor index were assessed using Brassica juncea as a model plant. Inoculation with Bacillus subtilis SRCM 121379 significantly increased the length of B. juncea shoots and roots by 45.6% and 52.0%, respectively. Among the bacterial isolates, Bacillus subtilis SRCM 121379 showed the superior enzyme activities, antagonistic capacity and plant growth promoting effects. Based on the experimental results, Bacillus subtilis SRCM 121379 (GenBank accession no. NR027552) was finally selected as a BCA candidate.

KCI등재 SCOPUS

4A Rapid and Universal Direct PCR Method for Macrofungi

저자 : Mi-jeong Park , Hyorim Lee , Rhim Ryoo , Yeongseon Jang , Kang-hyeon Ka

발행기관 : 한국균학회 간행물 : 한국균학회지 49권 4호 발행 연도 : 2021 페이지 : pp. 455-467 (13 pages)

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Macrofungi are valuable resources as novel drug candidates, new biomaterials, and edible materials. Recently, genetic approaches pertaining to macrofungi have been continuously growing for their identification, molecular breeding, and genetic engineering. However, purification and amplification of fungal DNA is challenging because of the rigid cell wall and presence of PCR inhibitory metabolites. Here, we established a direct PCR method to provide a rapid and efficient method for PCR-grade macrofungal DNA preparation applicable to both conventional PCR and real-time PCR. We first optimized the procedure of lysis and PCR using the mycelia of Lentinula edodes, one of the most widely consumed macrofungal species. Lysates prepared by neutralizing with (NH4)2SO4 after heating the mycelia in a mixture of TE buffer and KOH at 65℃ for 10 min showed successful amplification in both conventional and real-time PCR. Moreover, the addition of bovine serum albumin to the PCR mixture enhanced the amplification in conventional PCR. Using this method, we successfully amplified not only internal transcribed spacer fragments but also low-copy genes ranging in length from 500 to 3,000 bp. Next, we applied this method to 62 different species (54 genera) of macrofungi, including edible mushrooms, such as Pleurotus ostreatus, and medicinal mushrooms such as Cordyceps militaris. It was found that our method is widely applicable to both ascomycetes and basidiomycetes. We expect that our method will contribute to accelerating PCR-based approaches, such as molecular identification, DNA marker typing, gene cloning, and transformant screening, in macrofungal studies.

KCI등재 SCOPUS

5Microsatellite marker를 이용하여 송이감염묘로부터 발생한 송이 확인

저자 : 가강현 ( Kang-hyeon Ka ) , 김희수 ( Hee-su Kim ) , 이제완 ( Jei-wan Lee ) , 장영선 ( Yeongseon Jang ) , 유림 ( Rhim Ryoo )

발행기관 : 한국균학회 간행물 : 한국균학회지 49권 4호 발행 연도 : 2021 페이지 : pp. 469-476 (8 pages)

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송이는 상업적으로 중요한 야생 식용 버섯이다. 현재까지 송이 재배는 송이감염묘를 이용한 것이 유일한 방법이다. 2020년 송이감염묘로부터 21개의 송이가 홍천 송이시험지에서 발생하였다. 송이감염묘 생산지역의 송이와 이식한 송이감염묘로부터 발생한 송이는 모니터링을 통해 확인하였고, 13개의 microsatellite 마커를 이용하여 분석한 결과 동일한 것이 확인되었다. 송이감염묘는 송이균환 형성과 송이 발생에 성공하였고, 송이발생은 2010년과 2017년부터 2020년까지 발생하였다. 결론적으로 송이 버섯발생은 송이감염묘의 송이균에서 기원한 것이다.


Tricholoma matsutake is commercially important wild edible mushroom. The only method for cultivation of T. matsutake has been to use matsutake-infected pine trees. In this study, twenty-one fruiting bodies were collected from the matsutake-infected pine trees in the Hongcheon experimental site in 2020. The fruiting bodies from the existing production area of matsutake-infected pine tree and from its transplantation site were found to be identical through monitoring and analysis of 13 microsatellite markers. Transplanted matsutake-infected pine trees succeeded in fairy-ring formation and matsutake fruiting in 2010 and 2017 to 2020. In conclusion, the matsutake mycelium of transplanted matsutake-infected pine tree originates from the existing matsutake-infected pine tree production.

KCI등재 SCOPUS

6Novel Fungal Species Belonging to the Genus Acaulium Isolated from Riptortus clavatus (Heteroptera: Alydidae) in Korea

저자 : Ju-heon Lee , Leonid N. Ten , Seung-yeol Lee , Hee-young Jung

발행기관 : 한국균학회 간행물 : 한국균학회지 49권 4호 발행 연도 : 2021 페이지 : pp. 477-486 (10 pages)

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A survey of insect-associated fungi in Korea revealed a novel fungal strain isolated from the bean bug Riptortus clavatus (Heteroptera: Alydidae). Culturally and morphologically, the fungal strain designated KNUF-20-INY03, shares features with members of the genus Acaulium. Phylogenetic analyses based on the concatenated nucleotide sequences of the internal transcribed spacer regions (ITS) regions and partial sequences of the translation elongation factor 1-alpha (TEF1-α), and β-tubulin (β-TUB), and large subunit of the nuclear ribosomal RNA (LSU) genes showed that the isolate is part of a clade that includes other Acaulium species, but it occupies a distinct phylogenetic position. Based on the shape, size, and color of its conidia and conidiogenous cells, strain KNUF-20-INY03 is readily distinguishable from the closely related A. acremonium, A. albonigrescens, A. caviariformis, A. pannemaniae, and A. retardatum. The conidial length-to-width ratio (1.6) of the novel isolate is significantly lower than that of A. acremonium (1.9), A. albonigrescens (2.4), and A. pannemaniae (2.4), and KNUF-20-INY03 produces hyaline conidia and elliptical conidiogenous cells while A. caviariformis forms brown conidia and A. retardatum produces flask-shaped conidiogenous cells. Thus, both phylogenetic and morphological analyses indicate that this strain is a novel species in the genus Acaulium, and we propose the name Acaulium microspora sp. nov.

KCI등재 SCOPUS

7표고 담자포자 유래 단핵균사의 A 교배형과 생장 속도 상관관계

저자 : 박미정 ( Mi-jeong Park ) , 유림 ( Rhim Ryoo ) , 장영선 ( Yeongseon Jang ) , 가강현 ( Kang-hyeon Ka )

발행기관 : 한국균학회 간행물 : 한국균학회지 49권 4호 발행 연도 : 2021 페이지 : pp. 487-495 (9 pages)

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표고는 사극성의 교배계를 갖는 담자균의 일종으로, 표고의 교배형은 A와B라 불리는 서로 독립된 두 유전자좌에 의해 결정된다. 이론적으로 하나의 이핵균주는 네 개의 서로 다른 교배형을 갖는 담자포자를 1:1:1:1의 비율로 만들 수 있다. 과거 연구 결과에 따르면, 표고 담자포자에서 교배형이 편향된 분리비로 나타남이 보고되었다. 하지만 이러한 결과들은 꺽쇠연결과 같은 형태학적 특성만을 기반으로 교배형을 결정했다는 한계가 있다. 이에 본 연구에서는 교배형의 편향된 분리비가 표고에서 일반적인 현상인지 보다 명확하게 알아보기 위해서 최근에 보고된 DNA 마커를 활용하여 세 가지 표고 품종들의 담자포자에 대한 교배형 분석을 수행하였다. 그 결과 교배형의 편향된 분리비가 과거 보고와 일치하게 균주 특이적인 특성임을 확인하였다. 분석한 세 품종 중 한 품종을 제외하고 나머지 두 품종에서 편향된 분리비가 관찰된 것이다. 다음으로는 각 담자포자 유래 단핵균사들의 생장 속도와 교배형의 상관관계를 분석하였다. 그 결과 표고 단핵균사의 생장 속도는B 교배형과는 관계가 없고, A 교배형과 관계가 있음을 확인하였다. 따라서A 교배형 유전자좌에 존재하는 호메오도메인 전사인자 혹은A 교배형 유전자좌와 연관된 유전자들이 단핵균사의 생장에 영향을 줄 것으로 보인다. 버섯 신품종 육성에서 교배형의 중요성을 고려할 때, 본 연구는 효율적인 신품종 육성 전략을 세우거나 단핵균사 생장 기작을 이해하는 데 도움을 줄 것으로 기대된다.


Lentinula edodes is a tetrapolar basidiomycete and its mating type is determined by two unlinked genetic loci, A and B. Theoretically, one dikaryotic strain could produce basidiospores with four different mating types in a 1:1:1:1 ratio. Previous studies have described the skewed segregation ratio of mating types among basidiospores of L. edodes. However, they were based only on morphological characteristics, such as clamp connection, to determine mating types. To clarify whether the segregation distortion of mating types is a general phenomenon in L. edodes, we analyzed the mating types of basidiospores obtained from three cultivars of L. edodes using recently developed DNA markers. We found that the skewed segregation of mating types was strain-specific, as reported previously. Among the three cultivars, one cultivar showed balanced segregation, while the other two displayed distorted segregation. We also examined the relationship between mating type and mycelial growth rate of monokaryons derived from each basidiospore. It was found that the monokaryotic mycelial growth rate was related to the A mating type but not to the B mating type. Therefore, homeodomain transcription factor genes that reside on the A locus or other genes linked to the A locus affect the growth rate of monokaryotic mycelia. Considering the importance of mating types in mushroom breeding, this study is informative for establishing an efficient breeding strategy as well as for understanding the mechanism of monokaryotic mycelial growth.

KCI등재 SCOPUS

8참당귀에서 분리된 내생균의 다양성과 병원균 억제 효과

저자 : 박혁 ( Hyeok Park ) , 정충렬 ( Chung Ryul Jung ) , 엄안흠 ( Ahn-heum Eom )

발행기관 : 한국균학회 간행물 : 한국균학회지 49권 4호 발행 연도 : 2021 페이지 : pp. 497-505 (9 pages)

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다양한 지역에서 채취한 참당귀(Angelica gigas Nakai)의 잎, 줄기, 뿌리에서 내생균을 분리하였다. 내생균 균주는 형태적특징과 ITS (internal transcribed spacer) 지역의 염기서열을 이용하여 동정하였다. 총 35종의 내생균이 동정되었다. 내생균의 다양성은 참당귀의 조직에 따라 다르게 나타났다. 분리된 내생균은 참당귀 점무늬병균Phoma sp. Y11 균주와 대치배양하여 항균활성을 측정하였다. 총 14종의 내생균이 균주 Y11에 대해 억제효과를 나타내었다. 본 연구는 참당귀에서 분리한 내생균이 참당귀의 점무늬병에 대한 생물학적 방제제로 사용될 수 있다는 것을 제시하고 있다.


Endophytic fungal strains were isolated from leaves, stems, and roots of Angelica gigas. The fungal strains were identified based on their morphological characteristics and molecular analysis of the internal transcribed spacer (ITS). A total 35 species of endophytic fungi were identified. The diversity between the endophytic fungal communities differed depending on the tissues of A. gigas. The isolated endophytic fungi were screened for antifungal activity against a pathogenic fungus, Phoma sp. Y11, using a dual culture method. Fourteen species of endophytic fungi showed the standout inhibition effect against the Y11 strain. The results suggest that the endophytic fungi isolated from A. gigas could be used as a biological control agent against leaf spot disease of A. gigas.

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9단핵균주간 교잡에 의한 에르고티오네인 함량이 높은 기능성 표고 균주 선발

저자 : 김민준 ( Min-jun Kim ) , 정연석 ( Yeun Sug Jeong ) , 장영선 ( Yeongseon Jang ) , 가강현 ( Kang-hyeon Ka )

발행기관 : 한국균학회 간행물 : 한국균학회지 49권 4호 발행 연도 : 2021 페이지 : pp. 507-514 (8 pages)

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본 연구에서는 단포자 교배를 통해 교잡균주를 만들고 자실체를 발생시켜 형태적 특성과에 르고티오네인 함량을 조사하였다. 자실체의 형태적 특성과 생산량을 함께 고려한다면 교잡 균주 중 NIFoS 5101 균주의 자실체가 준수하게 확인되었다. 자실체 에르고티오네인 함량 조사 결과 모균주 평균이 540 mg/kg이었고 교잡균주 자실체 평균은 459 mg/kg으로 확인되었다. 대부분의 교잡균주 자실체는 모균주보다 낮은 에르고티오네인 함량을 보였지만 NIFoS 5101 균주 자실체는 모균주인 밤빛향보다 높게 측정되었으며 NIFoS 5108 균주 자실체 두 가지 모균주 밤빛향과 산산향보다 20%가량 높은 것을 확인할 수 있었다. 자실체의 형태적 특성과 에르고티오네인 함량 분석 결과를 종합하면 NIFoS 5101 균주가 교잡균주 중 가장 준수한 결과를 나타냈다.


Lentinula edodes have been cultivated extensively not only in South Korea but also in other Asian countries. In terms of taste and nutrition, it is a valuable mushroom that is comparable to other mushrooms. L. edodes contains various physiologically active compounds that promote human health. One compound, ergothioneine, exerts a powerful antioxidant function that inhibits cellular senescence. As it is not biosynthesized by plants or animals, L. edodes is very important for ergothioneine supply. The L. edodes cultivars Bambithyang (NIFoS 3404) and Sansanhyang (NIFoS 3420) of the National Institute of Forest Science (NIFoS) had higher ergothioneine content than the other cultivars, and 11 strains were obtained using mono-mono hybridization between them. The strains were inoculated into sawdust media to obtain fruiting bodies, and the ergothioneine content of each hybrid strain was confirmed using high-performance liquid chromatography (HPLC) analysis. Among the 11 strains, NIFoS 5108 had a higher ergothioneine content than the parental strains, Bambithyang (NIFoS 3404) and Sansanhyang (NIFoS 3420). This study revealed that it is possible to develop cultivars with improved specific functions using mono-mono hybridization.

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10Effects of Arbuscular Mycorrhizal Fungal Inoculation on the Growth of Red Pepper and Soil Glomalin Content

저자 : Ji-Eun Lee , Eun-hwa Lee , Ahn-heum Eom

발행기관 : 한국균학회 간행물 : 한국균학회지 49권 4호 발행 연도 : 2021 페이지 : pp. 517-524 (8 pages)

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Red pepper seedlings were inoculated either alone or with a mixture of all five species of arbuscular mycorrhizal fungi (AMF). After 10 weeks of growth in the greenhouse, the seedlings were transplanted into fields and cultivated without chemical fertilizers and pesticides for 10 weeks. The results showed that plant growth was significantly increased under both greenhouse and field conditions, suggesting that AMF inoculation has a positive effect on the growth of Capsicum annuum and improves the physical properties of the soil by increasing the concentration of glomalin. The application of AMF can positively contribute to sustainable agriculture by reducing the use of chemical fertilizers while increasing crop growth.

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