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한국미생물·생명공학회지 update

Microbiology and Biotechnology Letters

  • : 한국미생물생명공학회
  • : 자연과학분야  >  생물
  • : KCI등재
  • : SCOPUS
  • : 연속간행물
  • : 계간
  • : 1598-642x
  • : 2234-7305
  • : 산업미생물학회지(~2001)→한국미생물·생명공학회지(2002~)

수록정보
수록범위 : 30권1호(2002)~50권1호(2022) |수록논문 수 : 1,219
한국미생물·생명공학회지
50권1호(2022년 03월) 수록논문
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KCI등재 SCOPUS

1Acyl Homoserine Lactone in Interspecies Bacterial Signaling

저자 : Poonam Kanojiya , Rajashri Banerji , Sunil D. Saroj

발행기관 : 한국미생물생명공학회 간행물 : 한국미생물·생명공학회지 50권 1호 발행 연도 : 2022 페이지 : pp. 1-14 (14 pages)

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Bacteria communicate with each other through an intricate communication mechanism known as quorum sensing (QS). QS regulates different behavioral aspects in bacteria, such as biofilm formation, sporulation, virulence gene expression, antibiotic production, and bioluminescence. Several different chemical signals and signal detection systems play vital roles in promoting highly efficient intra- and interspecies communication. Gram-negative bacteria coordinate gene regulation through the production of acyl homoserine lactones (AHLs). Gram-positive bacteria do not code for AHL production, while some gram-negative bacteria have an incomplete AHL-QS system. Despite this fact, these microbes can detect AHLs owing to the presence of LuxR solo receptors. Various studies have reported the role of AHLs in interspecies signaling. Moreover, as bacteria live in a polymicrobial community, the production of extracellular compounds to compete for resources is imperative. Thus, AHL-mediated signaling and inhibition are considered to affect virulence in bacteria. In the current review, we focus on the synthesis and regulation mechanisms of AHLs and highlight their role in interspecies bacterial signaling. Exploring interspecies bacterial signaling will further help us understand host-pathogen interactions, thereby contributing to the development of therapeutic strategies intended to target chronic polymicrobial infections.

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2Anti-Inflammatory Effects of the Chemical Compounds Obtained from Celastrus hindsii in RAW264.7 Cells

저자 : Thi Ngoc Anh Pham , Hae Lim Kim , Suwon Oh , Seung Hwan Yang

발행기관 : 한국미생물생명공학회 간행물 : 한국미생물·생명공학회지 50권 1호 발행 연도 : 2022 페이지 : pp. 15-21 (7 pages)

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Celastrus hindsii (family Celastraceae) is located abundantly in the United States, China, and Vietnam, where it is utilized as a traditional herbal and traditional drug for the care of cancer. However, the antioxidant and anti-inflammatory effects of Celastrus hindsii extract are unknown. In our research, the antioxidant activity of Celastrus hindsii leaf extract was investigated, and then anti-inflammatory efficacy of C. hindsii extract in lipopolysaccharide (LPS)-induced RAW264.7 cells. First, our results revealed that C. hindsii extract showed powerful antioxidant capability. Moreover, the application of C. hindsii extract significantly reduced nitric oxide (NO) production without cytotoxic effects. Furthermore, C. hindsii extract reduced the generation of pro-inflammatory cytokines, like as interleukin (IL)-6, IL-1β, and TNF-α. Our results are the first to confirm the anti-inflammatory capability of C. hindsii extract in RAW264.7 cells.

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3Anti-Inflammatory Effects of Abalone (Haliotis discus hannai) Viscera via Inhibition of ROS Production in LPS-Stimulated RAW 264.7 Cells

저자 : Tai-sun Shin , Kap Seong Choi , Jiyeon Chun , Kang-hee Kho , Seon Ah Son , Sun-yup Shim

발행기관 : 한국미생물생명공학회 간행물 : 한국미생물·생명공학회지 50권 1호 발행 연도 : 2022 페이지 : pp. 22-30 (9 pages)

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Haliotis discus hannai called abalone, is the valuable marine mollusks and the by-products of abalone processing are viscera. Brownish abalone male viscera (AMV), which have not been reported as having anti-inflammatory effects, was extracted with acetone and fractionated by different six acetone/hexane ratios (0, 10, 20, 30, 40, and 100%) using a silica column via in vitro ABTS and DPPH radical and nitric oxide (NO) production assay-guided fractionation. Among the fractions, the acetone/hexane ratio 40%, A40 exhibited the most potent radical scavenging activities and inhibition of lipopolysaccharide (LPS)-induced NO production without cytotoxicity. A40 inhibited LPS-induced intracellular reactive oxygen species (ROS) production in a dose-dependent manner. Western blot analysis revealed that A40 down-regulated the activation of NF-κB, MAPK (ERK 1/2, p-38, and JNK), and inflammatory enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2. Moreover, this fraction inhibited the generation of pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α. These results suggested that AMV containing A40 with anti-inflammatory and anti-oxidantive effects, is the effective therapeutic and functional material for treating inflammatory disorders.

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4Melanin 생성 인자 억제 효과를 통한 Mangifera indica L. Peel의 미백효과 연구

저자 : 김효민 ( Hyo-min Kim ) , 유단희 ( Dan-hee Yoo ) , 이인철 ( In-chul Lee )

발행기관 : 한국미생물생명공학회 간행물 : 한국미생물·생명공학회지 50권 1호 발행 연도 : 2022 페이지 : pp. 31-39 (9 pages)

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본 연구의 목적은 애플망고 껍질의 열수 및 70% 에탄올 추출물의 미백 효과를 검증하고자 하였다. 애플망고 껍질의 열수 및 에탄올 추출물의 미백 효과를 측정하기 위해 tyrosinase 저해 활성을 측정한 결과, 최종 농도인 1,000 μg/ml에서 열수 추출물은 9%, 에탄올 추출물은 35%의 저해 효과를 보였다. 세포를 통해 미백효과를 측정하고자 멜라노마 세포인 B16-F10을 이용해 세포 생존율을 MTT assay를 사용하여 측정하였다. 세포 생존율 측정 결과, 100 μg/ml 농도에서 각각 95.64%, 103.36%의 세포 생존율을 나타내었다. 이후 실험은 세포 생존율이 95% 이상 나타난 농도인100 μg/ml 이하의 농도에서 실험을 수행하였다. 미백 효과는 멜라닌 합성에 관여하는 인자의 단백질 및 mRNA 발현을 측정하여 결정하였다. 단백질 발현은 western blot을 이용하여 측정하였으며, 그 결과 MITF, tyrosinase, TRP-1 및 TRP-2에 대한 단백질 발현은 100 μg/ml 농도에서 열수 추출물에 의해 59%, 65%, 26%, 18% 감소하였고, 에탄올 추출물에 의해 64%, 40%, 18%, 52% 감소하였다. MITF, Tyrosinase, TRP-1 및 TRP-2의 mRNA 발현은 RT-PCR을 통해 확인하였으며, 그 결과 100 μg/ml에서 열수 추출물에 의해 27%, 44%, 40%, 22% 감소하였고, 에탄올 추출물에 의해 9%, 51%, 11%, 52% 감소하였다. 따라서 애플망고 껍질 추출물이 미백 효과가 있음을 확인하였고, 천연물 소재로서의 이용 가치가 높을 것으로 사료되어진다.


The purpose of this study was to investigate the whitening effects of hot water (AMPW) and ethanol (AMPE) extracts of Mangifera indica L. peel. To verify the whitening effects, tyrosinase inhibitory activity was measured. 9.51% inhibitory activity, and 35.98% inhibitory activity at 1,000 μg/ml. The effects of AMPW and AMPE on cell viability were measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay in B16-F10 melanoma cells. Greater than 95% cell viability was observed at 100 μg/ml. Thus, subsequent experiments were performed at concentrations less than 100 μg/ml. The whitening effects were confirmed by measuring the protein and mRNA expression levels of microphthalmia-associated transcription factor, tyrosinase, tyrosinase-related protein 1 (TRP-1), and TRP-2, which are factors involved in melanin synthesis. Western blotting and reverse transcription-polymerase chain reaction results confirmed that 100 μg/ml AMPW and AMPE showed superior inhibitory effects than the control treatment (alpha-melanocyte stimulating hormone only). Therefore, Mangifera indica L. peel extract had a whitening effect, and thus, has potential as a natural material for use in cosmetics.

KCI등재 SCOPUS

5Mycorrhizal Synthesis of Perigord Black Truffle (Tuber melanosporum) with Mexican Oak Species

저자 : Gonzalo Guevara-guerrero , Giovanni Pacioni , Marco Leonardi , Fortunato Garza Ocañas , Rigoberto Gaitán Hernández

발행기관 : 한국미생물생명공학회 간행물 : 한국미생물·생명공학회지 50권 1호 발행 연도 : 2022 페이지 : pp. 40-50 (11 pages)

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The Périgord black truffle (Tuber melanosporum) is an edible fungus and among the most expensive foods worldwide. It is the basis of a multimillion-dollar bio-business. Truffle farming does not exist in Mexico, and no formal studies have been conducted on its culture. This report describes the mycorrhizal synthesis (i.e., artificial union of fungus with host) of T. melanosporum with oak species native to Mexico (Quercus polymorpha, Q. fusiformis, and Q. canbyi). The mycorrhizal association was successful in Q. polymorpha and Q. fusiformis, as confirmed morphologically and using T. melanosporum molecular primers (ITSML/ ITS4LNG). The effect of the ectomycorrhizal fungus on host growth (stem diameter) was statistically significant. Illustrations of the study are presented.

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6Potential Probiotic Characteristics and Safety Assessment of Lactobacillus rhamnosus SKG34 Isolated from Sumbawa Mare's Milk

저자 : I Nengah Sujaya , Gede Ngurah Rsi Suwardana , Kazuyoshi Gotoh , I Wayan Sumardika , Komang Ayu Nocianitri , Ni Putu Sriwidyani , I Wayan Gede Artawan Eka Putra , Masakiyo Sakaguchi , Ni Nengah Dwi Fatmawati

발행기관 : 한국미생물생명공학회 간행물 : 한국미생물·생명공학회지 50권 1호 발행 연도 : 2022 페이지 : pp. 51-62 (12 pages)

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Lactobacillus rhamnosus SKG34 (LrSKG34), a potential probiotic strain, was successfully isolated from Sumbawa Mare's milk. Our previous studies showed that the strain is resistant to gastrointestinal conditions, possesses antioxidant activity, and lowers blood cholesterol levels. Further clarification of the potential probiotic characteristics and safety assessment are necessary. This study aimed to evaluate the adhesion of LrSKG34 to Caco-2 cell monolayers and its effect on mucosal integrity in vitro. We also examined the LrSKG34 safety profile based on antimicrobial susceptibility testing, haemolytic activity determination, Caco-2 cell monolayer translocation evaluation, and in vivo investigation of the effect of LrSKG34 on the physiology, biochemical markers, and histopathological appearance of major organs in an animal model. LrSKG34 attached to Caco-2 cell monolayers and maintained mucosal integrity in vitro. The typical resistance of lactobacilli to ciprofloxacin, gentamicin, vancomycin, trimethoprim-sulfamethoxazole, and metronidazole was confirmed for LrSKG34. No haemolytic activity was observed on blood agar plates, and no LrSKG34 translocation was observed in Caco-2 cell monolayers. Administration of LrSKG34 to Sprague-Dawley rats did not adversely affect body weight. No abnormalities in hematological parameters, serum biochemistry levels, or histopathological structures of major organs were observed in LrSKG34-treated rats. Collectively, the results implicate LrSKG34 as a promising and potentially safe probiotic candidate for further development.

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7Characterization of Two Cryptic Plasmids from Levilactobacillus zymae GU240

저자 : Huong Giang Le , Min Jae Kim , Hye Sung Jeon , Ji Yeon Yoo , Yun Ji Kang , Tae Jin Kim , Jeong Hwan Kim

발행기관 : 한국미생물생명공학회 간행물 : 한국미생물·생명공학회지 50권 1호 발행 연도 : 2022 페이지 : pp. 63-70 (8 pages)

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Two small cryptic plasmids, pHG1 and pHG2, were isolated from Levilactobacillus zymae (formerly Lactobacillus zymae) GU240 and characterized. pHG1 is 1,814 bp in size with a GC content of 37.4% and contains two open reading frames. orf1 can potentially encode a protein of 101 amino acids (aa) with 99% identity with the copy number control protein of Lacticaseibacillus paracasei. orf2 can potentially encode a protein of 230 aa with 99% identity with a replication protein from multiple species. Six inverted repeats (IR I-VI) and six direct repeats (DR I-VI) were found in pHG1. pHG2 is 2,864 bp in size, with a GC content of 39.6%. pHG2 has two orfs. orf1 might encode a protein with 99% identity with the TrsL transmembrane protein. orf2 might encode a protein with 99% identity with plasmid recombination proteins from lactic acid bacteria. Both pHG1 and pHG2 may be useful as frames for constructing lactic acid bacteria-Escherichia coli shuttle vectors.

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8Purification, Characterization and Application of a Cold Active Lipase from Marine Bacillus cereus HSS

저자 : Sahar Wm. Hassan , Hala H. Abd El Latif , Ehab A. Beltagy

발행기관 : 한국미생물생명공학회 간행물 : 한국미생물·생명공학회지 50권 1호 발행 연도 : 2022 페이지 : pp. 71-80 (10 pages)

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Lipases (triacylglycerol acylhydrolases [EC 3.1.1.3]) are water-soluble enzymes. They catalyze the hydrolysis of fats and oils. A cold-active lipase from marine Bacillus cereus HSS, isolated from the Mediterranean Sea, Alexandria, Egypt, was purified and characterized. The total purification depending on lipase activity was 438.9 fold purification recording 632 U/mg protein. The molecular weight of the purified lipase was estimated to be 65 kDa using sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. The optimum substrate concentration, enzyme concentration, pH, and temperature were 1.5 mM, 100 μl, pH 6 and 10℃, respectively. The lipase was tolerant to NaCl concentrations ranging from 1.5 to 4.5%. The lipase was affected by the tested metal ions, and its activity was inhibited by 16% in the presence of 0.05 M SDS. The application of the cold-active lipase for the removal of an oil stain from a white cotton cloth showed that it is a promising biological agent for the treatment of oily wastes and other related applications. To the best of our knowledge, this is the first report of the purification and characterization of a lipase from marine B. cereus HSS isolated from the Mediterranean Sea.

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9Molecular Identification and Technological Properties of Acetic Acid Bacteria Isolated from Malatya Apricot and Home-Made Fruit Vinegars

저자 : Eda Buyukduman , Hatice Ebrar Kirtil , Banu Metin

발행기관 : 한국미생물생명공학회 간행물 : 한국미생물·생명공학회지 50권 1호 발행 연도 : 2022 페이지 : pp. 81-88 (8 pages)

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Acetic acid bacteria (AAB) are versatile organisms involved in the production of variety of fermented foods, such as vinegar and kombucha, and products of biotechnological relevance, such as bacterial cellulose. In the present study, Malatya apricot, a variety with protected designation of origin (PDO), and vinegar samples produced using various fruits were used to isolate AAB. The 19 AAB isolates obtained were typed using (GTG)5 fingerprinting, and the ones selected were identified by sequencing either 16S rDNA alone or in combination with 16S-23S rRNA internal transcribed spacer region or ligA gene. While all apricot isolates (n = 10) were Gluconobacter cerinus, vinegar isolates (n = 9) were composed of Komagataeibacter saccharivorans, Acetobacter syzygii, and possible two new species of AAB, Komagataeibacter sp., and Gluconobacter sp. (GTG)5 fingerprinting showed the presence of several genotypes of G. cerinus in the apricot samples. Screening for some technologically relevant properties, including thermotolerance, ethanol tolerance, and cellulose production capability, showed that all Komagataeibacter and some Gluconobacter isolates could tolerate the temperature of 35℃, and that vinegar isolates could tolerate up to 8% ethanol. One isolate, Komagataeibacter sp. GUS3 produced bacterial cellulose (1 g/l) and has the potential to be used for cellulose production.

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10Triclosan Resistant Bacteria from Sewage Water: Culture Based Diversity Assessments and Co-Resistance Profiling to Other Antibiotics

저자 : Muhmmad Salman , Noor Ul Bashar , Uzma Kiran , Zuhra Shafiq , Fareesa Khan , Raees Khan , Farrukh Hussain , Sudhair Abbas Bangash , Yasin Ahmad , Shabir Ahmad

발행기관 : 한국미생물생명공학회 간행물 : 한국미생물·생명공학회지 50권 1호 발행 연도 : 2022 페이지 : pp. 89-94 (6 pages)

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Triclosan (TCS) is an antimicrobial agent used in various human personal care products against both gram-positive and gram-negative bacteria. The purpose of this study was to evaluate the presence of TCS-resistant bacteria in sewage water in Peshawar, Khyber Pakhtunkhwa (KPK), Pakistan, for the first time. TCS-supplemented Luria Bertani (LB) agar was used to isolate TCS-tolerant bacteria. A total of 17 TCS-resistant isolates were randomly selected from a large pool of bacteria that showed growth on TCS-supplemented LB agar. Based on gram staining and physiochemical characteristics, the isolated strains were identified as Salmonella typhi (n = 6), Escherichia coli (n = 4), Citrobacter freundii (n = 4), Proteus mirabilis (n = 1), Enterobacter cloacae (n = 1), and Pseudomonas aeruginosa (n = 1). The Triclosan mean minimum inhibitory concentrations (MICs) for the isolates of Salmonella typhi, Escherichia coli, Citrobacter freundii, Proteus mirabilis, Enterobacter cloacae, and Pseudomonas aeruginosa were 23.66 μg ml-1, 18.75 μg ml-1, 42 μg ml-1, 32 μg ml-1, 64 μg ml-1, and 128 μg ml-1, respectively. The antibiogram revealed that all isolates were resistant to penicillin G (100%) and linezolid (100%), followed by ampicillin (94%), tetracycline (76%), tazobactam (76%), sulbactam/cefoperazone (64%), polymyxin PB (58%), amikacin (29.41%), aztreonam (29.41%), imipenem (5%), and gentamicin (5%). This is the first known study regarding the isolation of TCS-tolerant bacteria from sewage water in Peshawar, KPK, Pakistan. It was concluded that all the TCS-resistant isolates were multidrug resistant (MDR) gram-negative rod-shaped bacteria, mostly belonging to the Enterobacteriaceae family.

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