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한국미생물·생명공학회지 update

Microbiology and Biotechnology Letters

  • : 한국미생물생명공학회
  • : 자연과학분야  >  생물
  • : KCI등재
  • : SCOPUS
  • : 연속간행물
  • : 계간
  • : 1598-642x
  • : 2234-7305
  • : 산업미생물학회지(~2001)→한국미생물·생명공학회지(2002~)

수록정보
수록범위 : 30권1호(2002)~50권4호(2022) |수록논문 수 : 1,264
한국미생물·생명공학회지
50권4호(2022년 12월) 수록논문
최근 권호 논문
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KCI등재 SCOPUS

저자 : Kyung-soo Lee , Seo Young Park , Moo-seung Lee

발행기관 : 한국미생물생명공학회 간행물 : 한국미생물·생명공학회지 50권 4호 발행 연도 : 2022 페이지 : pp. 441-456 (16 pages)

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Shiga toxins (Stxs) are major virulence factors from the enterohemorrhagic Escherichia coli (EHEC), a subset of Stx-producing Escherichia coli. Stxs are multi-functional, ribosome-inactivating proteins that underpin the development of hemolytic uremic syndrome (HUS) and central nervous system (CNS) damage. Currently, therapeutic options for the treatment of diseases caused by Stxs are limited and unsatisfactory. Furthermore, the pathophysiological mechanisms underpinning toxin-induced inflammation remain unclear. Numerous works have demonstrated that the various host ribotoxic stress-induced targets including p38 mitogen-activated protein kinase, its downstream substrate Mitogen-activated protein kinase-activated protein kinase 2, and apoptotic signaling via ER-stress sensors are activated in many different susceptible cell types following the regular retrograde transportation of the Stxs, eventually leading to disturbing intercellular communication. Therapeutic options targeting host cellular pathways induced by Stxs may represent a promising strategy for intervention in Stx-mediated acute renal dysfunction, retinal damage, and CNS damage. This review aims at fostering an in-depth understanding of EHEC Stxs-mediated pathogenesis through the toxin-host interactions.

KCI등재 SCOPUS

저자 : Zain Ul Abedien , Kainat Gul , Maheen Shafiq , Khizar Rahman

발행기관 : 한국미생물생명공학회 간행물 : 한국미생물·생명공학회지 50권 4호 발행 연도 : 2022 페이지 : pp. 457-464 (8 pages)

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A public health concern emerging from a zoonotic disease. Monkeypox is caused by the orthopoxvirus specie Monkeypox virus (MPXV). Monkeypox was identified as the most common orthopoxvirus infection in humans following the eradication of smallpox. Monkeypox has a similar clinical presentation to smallpox. The MPXV is now considered a high-threat pathogen that causes a serious public-health problem. The continuous spread of Monkeypox virus from West Africa to all other places around the world throughout 2018 to 2022, have raised concerns that MPXV could have emerged to acquire the immunological and ecological niche vacated by smallpox virus. This review highlights the current knowledge about Monkeypox evolution, infection biology, and epidemiology around the world since from 1970 to 2022, with a focus on the human, viral, and cellular factors that influence virus emergence, infection, spread, and maintenance in nature. This paper also discusses the current therapeutic options for Monkeypox treatment and control. Under the right conditions, with limited smallpox vaccination and very little orthopoxvirus immunity in some areas of the world, MPXV could become a more efficient human pathogen. Finally, the review identified knowledge gaps, particularly in terms of identifying a definitive reservoir host for monkeypox and proposes future research endeavors to address the unanswered questions.

KCI등재 SCOPUS

저자 : Hyeon Ji Jeon , O-hyun Ban , Won Yeong Bang , Jungwoo Yang , Young Hoon Jung

발행기관 : 한국미생물생명공학회 간행물 : 한국미생물·생명공학회지 50권 4호 발행 연도 : 2022 페이지 : pp. 465-476 (12 pages)

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The importance of beneficial microorganisms, particularly probiotics, that coexist in the human body, is being increasingly recognized. Probiotics are representative health functional foods that provide health benefits to humans through the production of various metabolites, including short-chain fatty acids. However, the health benefits are strain-specific, and the use of each probiotic strain should follow guidelines that assure its safety. Accurate identification of the strain should be managed through genetic and phenotypic analyses of the strain. Besides, the functionality of probiotics should be disclosed in vitro and in vivo so that they can be used as legal functional ingredients (i.e., individual standards). In this review, we deal with the guidelines, including the technical factors related to probiotic strains. The common health effects of probiotic strains include proliferation of beneficial bacteria, control of harmful bacteria, and facilitation of bowel activities. Probiotics with various functionalities (e.g., body fat and cholesterol reduction, vaginal health, and improvement of skin's immune system) have been investigated as “individual standards of raw materials for health functional foods” provided by MFDS. In the future, various biotechnologies including synthetic biology can be applied to produce customized probiotics to improve human health.

KCI등재 SCOPUS

저자 : Dong-jin Kim , Tai Yeub Kim , Yeo-sang Yoon , Yongku Ryu , Myung Jun Chung

발행기관 : 한국미생물생명공학회 간행물 : 한국미생물·생명공학회지 50권 4호 발행 연도 : 2022 페이지 : pp. 477-487 (11 pages)

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Probiotics provide a symbiotic relationship and beneficial effects by balancing the human intestinal microbiota. The relationships between microbiota changes and various diseases may predict health abnormalities and diseases. Treatment with vitamins and probiotics is one therapeutic approach. To evaluate the effect of probiotics on vitamin absorption, we chose Lactobacillus rhamnosus CBT-LR5 treatment, which has resistance to vitamin C-inducible toxicity, with vitamins in high-fat diet (HFD)-induced obesity models. CBT-LR5 affected the absorption of micronutrients, such as ionic minerals and water-soluble vitamins. An increase in vitamin C absorption by CBT-LR5 enhanced the antioxidant response in HFD-induced obesity models. Increased vitamin B absorption by CBT-LR5 regulated lipid metabolism in HFD-induced obesity models. These favorable effects of CBT-LR5 on the absorption of vitamins should be investigated as candidate therapeutic target treatments for metabolic diseases.

KCI등재 SCOPUS

저자 : Noorulhuda Ojaimi Mahdi Al-dahlaki , Safaa Al-deen Ahmed Shanter Al-qaysi

발행기관 : 한국미생물생명공학회 간행물 : 한국미생물·생명공학회지 50권 4호 발행 연도 : 2022 페이지 : pp. 488-500 (13 pages)

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Most fungal infections by opportunistic yeast pathogens such as Candida spp. are the major causes of morbidity and mortality in patients with lowered immune. Previous studies have reported that some strains of Candida secret secondary metabolites play an important role in the decreasing of immunity in the infected patient. In this study, 110 Candida spp. were isolated from different clinical specimens from Baghdad hospitals. Candida isolates were identified by conventional methods, they were processed for Candida speciation on CHROMagar. The results of identification were confirmed by internal transcribed spacer (ITS) sequencing. Phylogenetic trees were analyzed with reference strains deposited in GenBank. Antifungal susceptibility testing was evaluated by the disc diffusion method and performed as recommended by the Clinical and Laboratory Standard Institute (CLSI) M44-A document. Candida isolates investigated produce secondary metabolites gliotoxin with HPLC technique and quantification. Out of 110 Candida isolates, C. albicans (66.36%) was the most frequent isolate, followed by the isolates of C. tropicalis (10.9%) and C. glabrata (6.36%) respectively. Concerning the antifungal susceptibility test, Candida isolates showed a high level of susceptibility to Miconazole (70.9%), Itraconazole (68.2%), and Nystatine (64.5%). The ability of obtained isolates of Candida spp. to produce gliotoxin on RPMI medium was investigated, only 28 isolates had the ability to secret this toxin in culture filtrates. The highest concentrations were detected in C. albicans (1.048 μg/ml). Gliotoxin productivity of other Candida species was significantly lower. The retention time for gliotoxin was approximately 5.08 min.

KCI등재 SCOPUS

저자 : 양수빈 ( Soo-been Yang ) , 유연진 ( Yeon-jin Yoo ) , 김응수 ( Eung-soo Kim ) , 최시선 ( Sisun Choi )

발행기관 : 한국미생물생명공학회 간행물 : 한국미생물·생명공학회지 50권 4호 발행 연도 : 2022 페이지 : pp. 501-507 (7 pages)

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대표적인 플라스틱 소재인 PET (Polyethylene terephthalate)는 높은 내구성, 경제성과 같은 유용한 물리화학적 특성으로 병, 섬유, 용기 등 다양한 산업 분야에 사용되고 있다. 최근 일회용품을 비롯한 플라스틱 사용량 증가로 인해, 이를 처리하기 위한 방법이 필요한 상황이다. 기존의 매립, 소각 등과 같이 자연상태에 노출되는 방법과 달리 최근 미생물을 이용한 친환경적인 방법이 주목받고 있다. 본 연구에서는 PETase 유전자를 가지고 있는 토양 유래 방선균 Streptomyces. javensis Inha503를 선별하고, agar plate diffusion assay를 통해 PU (Polyurethane) 가수분해 능력을 확인하였다. 해당 균주를 PET과 함께 한달 간 배양하였고, 주사전자현미경을 통해 PET 분해능력을 확인하였다. 또한, S. javensis Inha503 유전체 탐색에서 선별된 PETase 유전자를 PET 분해능이 없는 이종숙주 S. lividans와 S. coelicolor 균주에 도입하여 PET 분해능을 확인함으로써, 방선균 유래 PETase 유전자의 활성을 최초로 확인하였다.


PET (Polyethylene terephthalate), a representative plastic material, has useful physicochemical properties such as high durability and economic feasibility, and is used in various industrial fields such as bottles, fibers, and containers. Due to the recent increase in plastic usage including disposable products, ecofriendly strategy using microorganisms have drawn attention differentiated from conventional landfill and incineration methods. In this study, a soil-derived Streptomyces javensis Inha503 containing a PETase gene was selected and the ability to hydrolyze PU (Polyurethane) was confirmed through agar plate diffusion assay. This strain was cultured with PET for a month, and PET decomposition ability was also confirmed through a scanning electron microscope. Moreover, cloning and heterologous expression of S. javensis Inha503 PETase gene exhibited PET activity in the PETase non-containing S. coelicolor, confirming for the first time the presence of functional PETase gene in Streptomyces species.

KCI등재 SCOPUS

저자 : 김준영 ( Jun-young Kim ) , 한다희 ( Da-hee Han ) , 박근오 ( Geun-o Park ) , 남수완 ( Soo-wan Nam ) , 김연희 ( Yeon-hee Kim ) , 김한우 ( Han-woo Kim ) , 전숭종 ( Sung-jong Jeon )

발행기관 : 한국미생물생명공학회 간행물 : 한국미생물·생명공학회지 50권 4호 발행 연도 : 2022 페이지 : pp. 508-511 (4 pages)

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식물성 육류에서 미각적인 자극을 얻기 위해 사용하는 heme 함유 단백질의 지속적인 생산을 위해 강낭콩 유래 leghemoglobin a (PhLba) 유전자를 pPICZαA에 클로닝하고 Pichia pastoris에서 발현하였다. 재조합 PhLba 단백질은 가용화 형태로 배양 배지속으로 분비되었다. 정제된 PhLba의 분자량은 SDS-PAGE 상에서 16.5 kDa으로 나타났다. 재조합 PhLba holoprotein의 수율은 배양 배지에 hemin을 첨가함으로써 향상되었다. 이것은 PhLba의 apo 형태가 보조인자와 함께 효과적으로 포화된다는 것을 나타낸다.


In this study, Phaseolus vulgaris (kidney bean) leghemoglobin a (PhLba) gene was cloned into pPICZαA and expressed in Pichia pastoris to sustainably produce a heme-carrying protein for organoleptic use in plant-based meat. The recombinant PhLba protein was secreted into the culture medium in a solubilized form, and the molecular weight of the purified PhLba was estimated to be 16.5 kDa using SDS-PAGE. In addtion, the yield of recombinant PhLba holoprotein was enhanced by supplementation of the cultivation medium with hemin. This result indicates that the apo-forms of PhLba can be effectively saturated with cofactor.

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Since the main symptoms of COVID-19 involve the respiratory system, the infection rate of this disease is predicted to be higher in patients with other respiratory conditions such as allergic rhinitis. In such a situation, it will be meaningful to conduct research on an allergy treatment that has fewer side effects and can effectively reduce allergy symptoms. Here, we prepared experimental samples under various fermentation conditions with mixed extracts of six medicinal plants. To examine the anti-allergic efficacy of these samples, an egg albumin-induced allergic rhinitis animal model experiment, a serum histamine and IgE experiment, and a COX and LO inhibitory activity experiment were conducted. As a result of animal experiments, OVA+SP-4 showed superior efficacy compared to OVA+SP-1 in nasal rubbing and sneezing experiments and had anti-allergic efficacy similar to that of OVA-cetirizine. The serum histamine concentration of OVA+SP-4 was also 1.3 times higher than that of the OVA+cetirizine group, showing a high histamine reduction ability, and IgE showed the same trend. An analysis of COX inhibitory efficacy also confirmed that COX-1 and COX-2 inhibitory efficacy is high, and the longer the fermentation time, the higher the antiallergic efficacy. The composition proposed by this study is expected to have a significant effect on sustainable allergy prevention and treatment in the future by applying it to human patients.

KCI등재 SCOPUS

저자 : Jae Ho Choi , Jiyon Kim , Taekyun Shin , Myeong Seon Ryu , Hee-jong Yang , Do-youn Jeong , Hong-seok Son , Tatsuya Unno

발행기관 : 한국미생물생명공학회 간행물 : 한국미생물·생명공학회지 50권 4호 발행 연도 : 2022 페이지 : pp. 522-532 (11 pages)

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Cheonggukjang is a traditional fermented food in Korea, which is known to exert beneficial effects on health. In this study, we evaluated the effects of cheonggukjang fermented by Bacillus subtilis SCGB 574 (B574) on high fat diet (HFD)-deteriorated large intestinal health. Rats were fed with HFD or HFD supplemented with 10.1% cheonggukjang (B574). Fecal microbiota was analyzed based on 16S rRNA gene sequences, and the fecal and serum metabolome were measured using GC-MS. Our results showed that SCGB574 intake significantly reduced body weight, restored tight junction components, and ameliorated inflammatory cell infiltration. SCGB574 also shifted gut microbiota by increasing the abundance of short chain fatty acid producers such as Alistipes and Flintibacter, although it decreased the abundance of Lactobacillus. Serum and fecal metabolome analyses showed significantly different metabolic profiles between the groups. The top five metabolites increased by SCGB574 were i) arginine biosynthesis, ii) alanine, aspartate, and glutamate metabolism; iii) starch and sucrose metabolism; iv) neomycin, kanamycin, and gentamicin biosynthesis; and v) galactose metabolism. These results showed that cheonggukjang fermented by SCGB574 ameliorates adverse effects of HFD through improving intestinal health.

KCI등재 SCOPUS

저자 : Young Min Woo , Jae Yong Seo , Soo-ya Kim , Ji Hyun Cha , Hyun Dae Cho , Young Kwon Cha , Ju Tae Jeong , Sung Min Park , Hwa Sun Ryu , Jae Mun Kim , Moon Hoy Kim , Hee-taek Kim , Yong-min Kim , Kwang Si

발행기관 : 한국미생물생명공학회 간행물 : 한국미생물·생명공학회지 50권 4호 발행 연도 : 2022 페이지 : pp. 533-547 (15 pages)

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This study investigated the hair growth effect of Schisandra chinensis extract (TS-SC) and TS-SC fermented by Lactobacillus plantarum (TS-SCLF) on human dermal papilla cells (hDPCs). The production of vascular endothelial growth factor (VEGF), insulin-like growth factor 1 (IGF-1), keratinocyte growth factor/ fibroblast growth factor 7 (KGF/FGF-7) and hepatocyte growth factor (HGF), transforming growth factor beta 1 (TGF-β1) were examined. The secretion rates of VEGF and KGF/FGF-7 were high in TS-SC, and the secretion rates of IGF-1 and HGF were high in TS-SCLF. TGF-β1 was inhibited in a concentration-dependent manner in all samples. Gene expression of VEGF, IGF-1, KGF, HGF and alkaline phosphatase, relevant to hair growth, were examined. The data revealed that TS-SC and TS-SCLF successfully promoted hair growth in hDPCs. The IGF-1 gene was expressed in a dose-dependent manner in TS-SCLF. These results indicate that TS-SC and TS-SCLF fermented extract effectively promoted hair growth and gene expression relevant to hair growth in hDPCs. Used in clinical trials the test substance 'CMK-LPF01' showed a statistically significant increase in the number of hairs at 8 weeks, 16 weeks, and 24 weeks compared to before product use, and a change in hair growth, a secondary efficacy evaluation variable. Through additional research in the future, it is expected that “CMK-LPF01” can be developed as a functional material that can help alleviate symptoms of hair loss.

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