간행물

한국키틴키토산학회> 한국키틴키토산학회지

한국키틴키토산학회지 update

Journal of Chitin and Chitosan

  • : 한국키틴키토산학회
  • : 자연과학분야  >  생물
  • : KCI등재
  • :
  • : 연속간행물
  • : 계간
  • : 1229-4160
  • :
  • : 한국키틴키토산연구회지(~1998) → 한국키틴키토산학회지(1999~)

수록정보
수록범위 : 1권0호(1996)~27권2호(2022) |수록논문 수 : 1,106
한국키틴키토산학회지
27권2호(2022년 06월) 수록논문
최근 권호 논문
| | | |

KCI등재

저자 : Jiho Lee , Dong-jun Seo , Yong-su Song , Woo-jin Jung

발행기관 : 한국키틴키토산학회 간행물 : 한국키틴키토산학회지 27권 2호 발행 연도 : 2022 페이지 : pp. 59-68 (10 pages)

다운로드

(기관인증 필요)

초록보기

In this study, bacteria were isolated from rosemary rhizosphere and chitinolytic activity was detected in 0.2% chitin agar medium. Using 16s rRNA sequencing, the isolated bacteria were identified as Cellulomonas fimi. Chitinase, cellulase, and protease activities were found to increase with incubation time. Chitosanase activity was highest on day 1, and gradually declined thereafter. Crude enzyme of C. fimi was prepared and separated by SDS-PAGE and the expression patterns of enzyme activity for chitinase and chitosanase isozymes was studied. Three chitinase isozymes (36, 55, and 66 kDa) and one chitosanase (39 kDa) were detected SDS-PAGE gel combinded with glycol chitin and glycol chitosan, and these chitinase and chitosanase had different molecular weights. Indole acetic acid (IAA) production by C. fimi was analyzed in nutrient broth (NB) media containing different concentrations of tryptophan. IAA production was found to increase with increasing tryptophan concentration. Siderophore production by C. fimi was analyzed in colloidal chitin medium for 0, 1, 3, 5, and 7 d. The production of siderophore increased for 5 d and gradually decreased thereafter. The antifungal activities of C. fimi against G. zeae, F. solani, B. cinerea, F. oxysporum, C. cucumerinum, R. solani, and C. acutatum were investigated in potato dextrose broth medium containing chitin. C. fimi showed the highest antifungal activity against G. zeae.

KCI등재

저자 : Seo Yeong Park , Si-won Yoo , Seo Hee Lee , Jong Min Choi , Jin Woo Song , Eui Jeong Han , Ginnae Ahn

발행기관 : 한국키틴키토산학회 간행물 : 한국키틴키토산학회지 27권 2호 발행 연도 : 2022 페이지 : pp. 69-77 (9 pages)

다운로드

(기관인증 필요)

초록보기

In this study, we prepared enzymatic extracts using trypsin and pepsin from Hermetia illucens (THI and PHI) and evaluated their effects on oxidative stress in ultraviolet B (UVB)-stimulated keratinocytes. PHI showed a higher extraction yield than THI, but the protein content of THI was higher. When the antioxidant activity was assessed in vitro, both indicated an effect of 25% or more compared to the positive control (L-ascorbic acid). In addition, both THI and PHI decreased the generation of intracellular reactive oxygen species (ROS) in UVB-stimulated keratinocytes, without cytotoxicity at all concentrations used. We explored the efficacy of THI. Results demonstrated that THI effectively reduced apoptosis and sub-G1 DNA content. In addition, it was found that UVB-stimulated keratinocytes protect skin keratinocytes from UVB by regulating the apoptotic pathway, MAPKs, and NF-kB signaling pathways. In conclusion, these results show that trypsin hydrolysate of H. illucens protects cells from oxidative stress in UVB-irradiated human keratinocytes.

KCI등재

저자 : 한의정 ( Eui Jeong Han ) , 신은지 ( Eun-ji Shin ) , 한희진 ( Hee-jin Han ) , 안긴내 ( Ginnae Ahn ) , 안창범 ( Chang-bum Ahn )

발행기관 : 한국키틴키토산학회 간행물 : 한국키틴키토산학회지 27권 2호 발행 연도 : 2022 페이지 : pp. 78-84 (7 pages)

다운로드

(기관인증 필요)

초록보기

The purpose of this study was to investigate the inhibitory activities of a hot water extract from Ecklonia cava (ECH) against inflammation and oxidative damages in gluten-stimulated Caco-2 cells, a human colon epithelial cell. ECH improved the cell viability by reducing the generation of the intracellular reactive oxygen species (ROS) and the formation of apoptotic body in gluten stimulated Caco-2 cells. Also, ECH effectively decreased the mRNA expression level of cytokines such as interleukin (IL)-1β, IL-4, IL-8 and tumor necrosis factor (TNF)-α incresed by gluten stimulation in Caco-2 cells. In addition, ECH down-regulated the protein expression of pro apoptotic molecules, Bax, p53 and cleaved PARP, wherase up-regulated thoes of anti apoptotic molecule, Bcl-2 in gluten-stimulated Caco-2 cells. Moreover, ECH effectively suppressed the nuclear factor (NF)κB activation by regulating the phosphorylation of NFκB p65 and IκB-α in cytosol as well as the translocation of cytoplasmic NFκB p65 into the nucleus. Taken together, these results suggest that ECH may be a natural material with beneficial potentials for the improvement of inflammation and oxidative damages induced by gluten stimulation.

KCI등재

저자 : 김민경 ( Mingyeong Kim ) , 조치흥 ( Chi Heung Cho ) , 김세라 ( Sera Kim ) , 오미진 ( Mi-jin Oh ) , 박호영 ( Ho-young Park ) , 이상훈 ( Sang-hoon Lee )

발행기관 : 한국키틴키토산학회 간행물 : 한국키틴키토산학회지 27권 2호 발행 연도 : 2022 페이지 : pp. 85-93 (9 pages)

다운로드

(기관인증 필요)

초록보기

본 연구에서는 식품 첨가제로서 널리 사용되고 있으나 생물학적 기능성에 대한 연구가 부족한 ADSP의 장 상피 세포보호 효과를 확인하고자 하였다. 인간 유래의 장 상피 세포에 ADSP를 처리하였을 때 proliferation을 증가시켰으며, TNF-α 및 IFN-γ에 의해 증가한 IL-6와 IL-1β의 분비량을 유의적인 감소시켜 장 염증을 도움을 주는 것을 확인하였다. ADSP는 TNF-α 및 IFN-γ에 의해 손상된 장 상피 세포의 손상을 완화하고 증가한 투과도를 유의적으로 감소시키는 것을 확인하였다. 장 상피 세포의 장벽 기능 및 투과도에 중요한 역할을 하는 tight junction 단백질의 발현량의 ADSP의 처리에 따라 증가하는 것을 형광 이미지로 확인하였다. 따라서 ADSP는 염증으로부터 장 상피 세포를 보호하고 장벽 기능에 도움을 주는 것으로 보이며, 이와 관련된 추가적인 메커니즘 규명 연구가 필요할 것으로 사료된다.


The intestine digests the food we eat and regulates the absorption of nutrients as well protect the body from external antigen or toxic substances. This barrier function become abnormal during inflammation. In this study, we confirmed the anti-inflammatory and epithelial barrier protective properties of acetylated distarch adipate (ADSP) in human-derived intestinal epithelial cells. ADSP showed a significant increase in cell viability with pro-inflammatory cytokine treatment and non-treatment. IL-6 and IL-1β significantly decreased with ADSP treatment as compared to the inflammation-induced group, while IL-8 didn't show significant change. After confirming that ADSP significantly reduces the epithelial monolayer integrity damage and increased permeability induced by TNF-α and IFN-γ, we confirmed the change in expression amount of tight junction with immunofluorescence and western blotting. Our results contributed to the study of the bioactivity of ADSP, which is widely used as a food additive, and indicated its potential as an agent for inflammatory bowel disease.

KCI등재

저자 : Jio Song , Eung Take Lee , Ji Hyun Lee , Yong Hyun Lee , Jae Kweon Park

발행기관 : 한국키틴키토산학회 간행물 : 한국키틴키토산학회지 27권 2호 발행 연도 : 2022 페이지 : pp. 94-103 (10 pages)

다운로드

(기관인증 필요)

초록보기

The biological activity of chitosan differs depending on the molecular weight and degree of deacetylation, so more precise molecular weight determination has become more important. It is difficult to determine the absolute molecular weight size due to the lack of precision, whereas the approximate molecular size is determined using HPLC. Therefore, the hydrolysates using acid or enzyme were subjected to precise molecular weight analysis by using Matrix-Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF). For analysis, two types of matrices were used in this study. The results showed slight differences in sensitivity but were confirmed to be similar to each other. In addition, it was confirmed that any matrix used in this study did not significantly affect the results. In conclusion, the results of this study will provide very important scientific clues in the investigation of the biological activities of chitosan such as antibacterial, anticancer, immune, and antioxidant activity.

KCI등재

저자 : 이종민 ( Jongmin Lee ) , 서원정 ( Wonjeong Seo ) , 송명오 ( Myeong-oh Song ) , 오은찬 ( Eunchan Oh ) , 진정호 ( Jungho Jin )

발행기관 : 한국키틴키토산학회 간행물 : 한국키틴키토산학회지 27권 2호 발행 연도 : 2022 페이지 : pp. 104-108 (5 pages)

다운로드

(기관인증 필요)

초록보기

본 연구는 생분해성, 우수한 기계적 특성 등의 다양한 장점을 가졌음에도 강한 수소결합에 의해 나노섬유화에 어려움이 있는 β-키틴의 나노섬유화를 진행하였다. 이는 SA 처리를 이용한 화학적 전처리 방법과 ACC의 물리적 처리가 결합된 방법이다. 화학적 전처리는 ChNF 간의 전기적 반발력을 유도하며, 물리적 처리는 섬유다발의 β-키틴을 균일한 크기의 나노섬유로 나노섬유화 할 수 있다. 화학적 전처리와 물리적 처리가 수반된 SChNF는 ACC 약 100 pass를 거쳤을 때 가장 균일한 나노섬유화를 이루는 것을 확인하였다. SChNF은 나노섬유화 후에 키틴 고유의 결정 및 화학 구조를 유지하는 것을 FT-IR 및 XRD 분석을 통해 확인하였다. 나노섬유화 정도에 따라 SChNF 필름의 광학적 특성이 향상되었다.


In this study, we report on the production of succinylated β-chitin nanofibers (ChNF) via aqueous counter collision (ACC) method. Succinic anhydride (SA) mediated chemical modification is known as an effective nanofibrillation strategy for typical natural polysaccharides through the surface carboxylation, which has attracted attention due to its relatively mild characterisitics, such as non-toxicity. Using the combined physico-chemical treatment of SA and ACC, the succinylated β-chitin nanofiber (β-SChNF) was successfully prepared as a uniform hydrocolloidal suspension. The SEM and AFM analysis showed that the diameter of the SChNF ranged from 14 nm to 30 nm. The XRD and FT-IR analysis were used to confirm that the original crystal and chemical structure of chitin were maintained even after the physico-chemical nanofibrillation. We also fabricated a transparent film from the SChNF which showed a typical diameter-dependent optical transmittance; that is, the higher the number of ACC pass (i.e., smaller diameter of SChNF), the greater the optical transparency (80%). Our results show that SA chemical pre-treatment of β-chitin can boost successful nanofibrillation.

1
권호별 보기

내가 찾은 최근 검색어

최근 열람 자료

맞춤 논문

보관함

내 보관함
공유한 보관함

1:1문의

닫기