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Journal of Chitin and Chitosan

  • : 한국키틴키토산학회
  • : 자연과학분야  >  생물
  • : KCI등재
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  • : 연속간행물
  • : 계간
  • : 1229-4160
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  • : 한국키틴키토산연구회지(~1998) → 한국키틴키토산학회지(1999~)

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수록범위 : 1권0호(1996)~27권3호(2022) |수록논문 수 : 1,112
한국키틴키토산학회지
27권3호(2022년 09월) 수록논문
최근 권호 논문
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KCI등재

저자 : 최유성 ( Yoo-sung Choi ) , 정경원 ( Gyeong-won Jeong )

발행기관 : 한국키틴키토산학회 간행물 : 한국키틴키토산학회지 27권 3호 발행 연도 : 2022 페이지 : pp. 119-124 (6 pages)

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In order to improve the wettability of contact lenses and to impart antibacterial properties against P. aeruginosa, E. coli and S. aureus a major bacterium that causes keratitis, alginate, low molecular weight as a natural polysaccharide, was added. Contact lenses were manufactured using 2-hydroxyethyl methacrylate(2-HEMA), 1-vinyl-2-pyrrolidinone(NVP) and ethylene glycol dimethacrylate(EGDMA). The addition of low molecular weight alginate salt(LMWAS) was carried out by the IPN method. The manufactured contact lenses were checked for moisture content, light transmittance, and antibacterial properties. It was confirmed that the moisture content augmented when the amount of LMWAS was increased, and the light transmittance did not change significantly. The antibacterial effect and minimum inhibitory concentration(MIC) were examined in strain such as P. aeruginosa, E. coli and S. aureus. It was confirmed that the LMWAS(Mw: 4082 Da) was indicated a minimum inhibitory concentration(MIC) of 0.10% against P. aeruginosa, E. coli and S. aureus and it was confirmed that the same antibacterial properties were exhibited even after mixing with the actual lens composition. These results suggest that LMWAS-combined contact lenes can be utilized as functional lenes to protect corneal epithelium.

KCI등재

저자 : Yong-su Song , Woo-jin Jung

발행기관 : 한국키틴키토산학회 간행물 : 한국키틴키토산학회지 27권 3호 발행 연도 : 2022 페이지 : pp. 125-131 (7 pages)

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Chitinase and chitin oligosaccharide were purified and separated from the Pedobacter sp. PR-M6 culture medium using general and modified processes. The chitinase and chitin oligosaccharide yields were 0.274 mg and 133 mg/ 751 mg chitin with the general process and 0.403 mg and 410 mg/978.4 mg chitin with the modified process, respectively. In addition, PR-M6 chitinase activity in two purified chitinases (P-chitinase 1 and 2) at 25℃ and 37℃ was investigated. In the P-chitinase 1, chitinase activity was 43.5 unit/mg protein and 73.2 unit/mg protein under 25℃ and 37℃ enzyme reaction conditions, respectively. However, in P-chitinase 2, chitinase activity was 50.8 unit/mg protein and 84.4 unit/mg protein under 25℃ and 37℃, respectively. The PR-M6 chitinase isozymes were expressed as Chi-A, Chi-B, and Chi-C on sodium dodecyl sulfate-polyacrylamide gels at 25℃ and 3℃. Thin-layer chromatography revealed that the main chitin oligosaccharide products from PR-M6 were dimer, trimer, and tetramer molecules in all P-chitinase lanes and monomer, dimer, trimer and tetramer molecules in crude enzymes. Furthermore, the PR-M6 P-chitinase inhibited Alternaria brassicicola conidia germination and hyphal development.

KCI등재

저자 : 김영수 ( Yeong-su Kim ) , 박창수 ( Chang-su Park ) , 나채선 ( Chae Sun Na )

발행기관 : 한국키틴키토산학회 간행물 : 한국키틴키토산학회지 27권 3호 발행 연도 : 2022 페이지 : pp. 132-136 (5 pages)

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우리나라 자생식물이자 전통적으로 한약재로 널리 이용되어져 온 도라지(Platycodon grandiflorum)의 경제적이고 고부가가치화를 위한 기술 개발을 위한 추출 방법에 대한 연구를 수행하였다. 상업용 가수분해 효소 Viscozyme과 Termamyl 2X를 2:1 비율로 혼합된 복합 효소를 도라지 추출 시 같이 첨가함으로써 일반 열수 추출에 비해 추출 수율 및 조사포닌 함량이 각각 43%, 23% 증가될 뿐 아니라 기능성 사포닌으로 다양한 연구가 수행중인 platycodin D의 함량을 약 3배 증가시키는 결과를 얻어 도라지 추출물 제조에 있어 경제적일 뿐 아니라 부가가치를 높일 수 있는 효과적으로 방법으로 판단된다. 또한 이렇게 제조된 복합효소처리 도라지 추출물은 일반 도라지 열수 추출물에 비해 항산화 활성(DPPH 라디컬 소거능은 1.7배, 미백 활성은 1.4배, 주름개선 활성은 1.4배 증가하는 보여 일반식품, 건강기능식품, 화장품 원료 등 다양한 바이오 산업에 응용 가능할 것으로 사료된다.


The two glycoside hydrolases (Viscozyme and Termamyl 2X, commercial food grade enzymes) was used for production of balloon flower (Platycodon grandiflorum) extract. The complex enzyme was prepared in a ration of Viscozyme and Termamyl 2X of 2:1. The optimal temperature and concentration of complex enzyme was 50℃ and 5% (w/w). Under optimal conditions, the extraction yield, crude saponine, and platycodin D contents of complex enzyme treated Platycodon grandiflorum extract was 43, 24, and 303% higher than Platycodon grandiflorum hot water extract. And the DPPH radical scavenging activity, ABTS scavenging activity, tyrosinase inhibitory activity, and elastase inhibitory activity of the complex enzyme treated Platycodon grandiflorum extract exhibited 1.7, 1.3, 1.4, and 1.4 fold higher than Platycodon grandiflorum hot water extract. These results indicated that the complex enzyme treatment can be used as an economical method for industrial production Platycodon grandiflorum extract. And the complex enzyme treated Platycodon grandiflorum extract cad potentially be used for developing a new cosmetic or health food ingredients.

KCI등재

저자 : 서원정 ( Wonjeong Seo ) , 진정호 ( Jungho Jin )

발행기관 : 한국키틴키토산학회 간행물 : 한국키틴키토산학회지 27권 3호 발행 연도 : 2022 페이지 : pp. 137-142 (6 pages)

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자연계에 풍부한 천연 고분자로써 생분해성, 향균성 및 우수한 기계적 특성 등의 장점에도 불구하고 섬유들 사이 수소결합에 의한 불용성으로 활용에 제한이 있었던 α-키틴의 나노섬유화를 진행하였다. TEMPO 매개 산화를 통한 화학적 전처리 후 수중대향충돌(ACC)이라는 하향식(top-down) 물리적 나노섬유화 과정을 통해 물리 화학적 나노섬유화 방법으로 균일한 크기의 키틴 나노섬유(ChNF)를 확보하였다. TEMPO 매개 산화 시 산화제로 사용된 NaClO의 농도를 조절하여 산화시켰을 때, 30 mmol NaClO 사용 후 ACC 처리하였을 때 가장 균일한 키틴 나노섬유(ChNF)가 관찰되었다. TOCNF는 나노섬유화 후에도 키틴 고유의 화학 및 결정구조를 유지하고 있음을 FT-IR 및 XRD분석을 통해 확인하였다. 또한 사용된 NaClO의 농도에 따른 분산안정성을 제타전위를 통해, 필름의 광학적 특성을 투과도 측정을 통해 그 값이 키틴 나노섬유화 정도에 따라 향상됨을 확인하였다.


In this study, we reported on the nanofibrillation degree of TEMPO-oxidated α-chitin nanofibers (TOChNF) according to the oxidant NaClO concentration using aqueous counter collision (ACC) method as the means to implement the physical nanofibrillation of the TOChNF. We found that, for an equivalent ACC treatment level, the decrease of TOChNF diameter after TEMPO-mediated oxidation becomes more effective upon ACC treatment as the NaClO concentration is increased due to greater electrostatic repulsion between TOChNFs. Nevertheless, during the ACC treatment of the TOChNF, the original chemical and crystal structures were maintained. In addition, our Zeta potential analysis showed that the dispersion stability of TOChNF in aqueous suspension is improved as the NaClO concentration is increased. The solvent-casted film of the ACC-treated TOChNF also showed increased optical transmittance at higher NaClO concentration.

KCI등재

저자 : Dae Ok Kim , Dong Hwi Kim , Bo Sun Kim , Wonbong Lim

발행기관 : 한국키틴키토산학회 간행물 : 한국키틴키토산학회지 27권 3호 발행 연도 : 2022 페이지 : pp. 143-154 (12 pages)

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One of the more recent fracture-healing methods comprises applying patient-specific 3D printing. An optimal fracture-healing method would accurately print and embed a customized implant at the defect site of the fracture. In this study, to provide a standardized 3D printing process, the entire process of making a fracture model, manufacturing the customized 3D printing, and implanting the result was conducted through animal experiments. A fracture treatment 3D printing process was established and a fracture model was produced. X-ray and micro-computed tomography (CT) images were acquired and the portions with the fractured bones were extracted by segmentation and reconstruction using image preprocessing software. Based on this, 3D modeling of the fracture site scaffold was performed, and the 3D printing protocol was experimentally verified through polylactic acid (PLA)-based 3D printing and implantation at the fracture site. The entire process of making the fracture model, manufacturing the customized 3D-printed bone fragment, and implanting it in the defect site was performed based on 3D printing. There is a need for further animal clinical validation and histological studies on the fracture recovery process according to 3D printing protocols. Nevertheless, this approach (performing the implantation process of a customizable 3D printing scaffold for replacing atypical fractures using X-ray and micro-CT images) is expected to play a new role in fracture treatment.

KCI등재

저자 : 권수연 ( Su-yeon Kwon ) , 강익중 ( Ik-joong Kang )

발행기관 : 한국키틴키토산학회 간행물 : 한국키틴키토산학회지 27권 3호 발행 연도 : 2022 페이지 : pp. 155-162 (8 pages)

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The final goal of this research was to improve a treatment for allergy rhinitis using chitosan-hydrocortisone silver nanoparticles based on hydrocortisone-deposited chitosan. The nanoparticles were produced by depositing hydrocortisone in chitosan, generating a reaction with highly reactive silver, and placing the nanoparticles into the nasal mucosa. Silver nanoparticles were manufactured after chitosan deposition and the optimal hydrocortisone concentration supporting the highest drug delivery efficiency was identified. For efficient DDS(Drug Delivery Systems applications, an adsorption experiment was performed before silver nanoparticle production according to the hydrocortisone concentration. In addition, a release experiment with different amounts of silver salt was performed to determine the optimal hydrocortisone and silver salt concentrations for producing chitosan-hydrocortisone silver nanoparticles. The amount released was investigated as a parameter of time. When the hydrocortisone concentration was 0.05g, the chitosan adsorption rate was highest at 48%. A release experiment was conducted at 36℃ in PBS, which is similar to the human body condition, while changing the silver salt amount. At approximately 60 hours, the absorbance was constant, indicating that hydrocortisone was emitted from the chitosan particles. In the cases of the 50㎕ reaction and 100㎕ reaction, the release rate was 14.17% and 12.92%, respectively. Otherwise, in the case of using 10㎕ silver salt solution for the reaction, 99.6% of the amount absorbed was released, showing the highest efficiency as a drug delivery agent.

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