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Jouranl of Fish Pathology

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수록정보
수록범위 : 1권1호(1988)~33권2호(2020) |수록논문 수 : 801
한국어병학회지
33권2호(2020년 12월) 수록논문
최근 권호 논문
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1Production of virus-like particles of nervous necrosis virus displaying partial VHSV's glycoprotein at surface and encapsulating DNA vaccine plasmids

저자 : Jeong In Yang , Mariem Bessaid , Ki Hong Kim

발행기관 : 한국어병학회 간행물 : 한국어병학회지 33권 2호 발행 연도 : 2020 페이지 : pp. 103-109 (7 pages)

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In order to use nervous necrosis virus (NNV) virus-like particles (VLPs) as a delivery tool for heterologous antigens or plasmids, we attempted to produce red-spotted grouper nervous necrosis virus (RGNNV) VLPs displaying a partial region of viral hemorrhagic septicemia virus (VHSV) glycoprotein at the surface and VLPs that are harboring DNA vaccine plasmids within the VLP. A peptide encoding 105 amino acids of VHSV glycoprotein was genetically inserted in the loop region of NNV capsid gene, and VLPs expressing the partial part of VHSV glycoprotein were successfully produced. However, in the transmission electron microscope analysis, the shape and size of the partial VHSV glycoprotein-expressing NNV VLPs were irregular and variable, respectively, indicating that the normal assembly of capsid proteins was inhibited by the relatively long foreign peptide (105 aa) on the loop region. To encapsulate by simultaneous transformation with both NNV capsid gene expressing plasmids and DNA vaccine plasmids (having an eGFP expressing cassette under the CMV promoter), NNV VLPs containing plasmids were produced. The encapsulation of plasmids in the NNV VLPs was demonstrated by PCR and cells exposed to the VLPs encapsulating DNA vaccine plasmids showed fluorescence. These results suggest that the encapsulation of plasmids in NNV VLPs can be done with a simple one-step process, excluding the process of disassembly-reassembly of VLPs, and NNV VLPs can be used as a delivery tool for DNA vaccine vectors.

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2내수면 양식 어류에서 분리된 Edwardsiella 속 균주들의 유전학적 동정 및 생화학적 특성

저자 : 장문희 ( Mun Hee Jang ) , 김근용 ( Keun-yong Kim ) , 이유희 ( Yu Hee Lee ) , 오윤경 ( Yun Kyung Oh ) , 이정호 ( Jeong-ho Lee ) , 송준영 ( Jun-young Song )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 33권 2호 발행 연도 : 2020 페이지 : pp. 111-118 (8 pages)

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우리나라 내수면 양식 어류로부터 Edwardsiella속 세균 7개 균주를 분리하여 이들의 생화학적 특성 및 유전학적 특성을 조사하였다. 그 결과, 기존의 어류 병원성 세균으로 알려진 E. tarda 와 E. ictaluri 뿐 아니라, 최근 새로운 종으로 보고된 E.anguillarum과 E. piscicida를 성공적으로 분리 및 동정하여 우리나라 내수면 양식 어류에서 Edwardsiella속의 다양한 종이 분리되는 것을 확인하였다. 뱀장어류에서 분리된 4개 균주는 E. anguillarum, E. piscicida 및 E. tarda로, 메기와 동자개로부터 분리된 2개 균주는 E. ictaluri로, 버들치로부터 분리된 1개 균주는 E. piscicida로 동정되었다. 또한, 이들의 생화학적 특성의 조사 결과, 이들은 대부분 E. anguillarum, E. ictaluri, E. piscicida 및 E. tarda의 각 종에 해당하는 전형적인 생화학적 특성을 나타내었으며, 유전자를 이용한 분자계통발생학적 분석 결과와도 일치하였다. 특히, 16S rRNA 및 gyrB 유전자를 이용하여 Edwardsiella 속 종의 명확한 분류가 가능함을 확인함으로써, Edwardsiella속 세균의 분류학적 동정을 위한 마커로써의 가능성을 제시하였다. 본 연구의 결과, 우리나라 내수면 양식 어류로부터 다양한 Edwardsiella 속 종들이 분리되는 것을 확인하였으며, 이들 종들에 대한 체계적인 모니터링 및 숙주에 따른 병원성의 차이에 관한 연구가 필요함을 제안한다.


The genus Edwardsiella belonging to the family Enterobacteriaceae is a member of Gram-negative rod-shaped bacteria that cause disease in diverse aquatic organisms such as fish, amphibians and reptiles as well as avians and mammals including human throughout the world. This genus had been composed of three species, E. hoshinae, E. ictaluri and E. tarda, but recent researches erected two novel species, E. anguillarum and E. piscicida that were conventionally identified as E. tarda. In this study, we isolated seven strains belonging to the genus Edwardsiella from freshwater fishes that had been reared at inland fish farms in South Korea and investigated their biochemical characteristics and molecular phylogenetic relationships. The seven strains showed typical characteristics of four Edwardsiella species, E. anguillarum, E. ictaluri, E. piscicida and E. tarda, by biochemical analyses of Gram staining, indole and hydrogen sulfide (H2S) production, and API (Analytic Profile Index) 20E test. Molecular phylogenetic analyses inferred from DNA sequence data of both 16S ribosomal RNA (rRNA) and DNA gyrase subunit B (gyrB) genes were congruent with the biochemical characteristics. As a result, both biochemical and molecular phylogenetic analyses identified four strains isolated from three Anguilla species as E. anguillarum, E. piscicida and E. tarda, two strains from Pelteobagrus fulvidraco and Silurus asotus as E. ictaluri, and one strain from Moroco oxycephalus as E. piscicida. In this study, we isolated and successfully identified recently newly erected species, E. anguillarum and E. piscicida in addition to historically notorious pathogenic species, E. ictaluri and E. tarda. In the future study, systematic and comprehensive monitoring of the four Edwardsiella species are required for studying differences in pathogenicity among freshwater fishes.

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3제주지역 넙치(Paralichthys olivaceus) 연쇄구균병 원인체의 분리특성과 Streptococcus parauberis의 혈청형 변화

저자 : 김경욱 ( Kyeong Wook Kim ) , 유은호 ( Eun Ho Yoo ) , 양혜영 ( Hye Young Yang ) , 강봉조 ( Bong Jo Kang )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 33권 2호 발행 연도 : 2020 페이지 : pp. 119-125 (7 pages)

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2013년부터 2020년까지 제주지역 양식 넙치로 부터 연쇄구균 470 균주를 분리하였다. 분리된 균주를 대상으로 multiplex PCR방법을 이용한 종 동정결과 S. iniae가 92균주(19.6%), S. parauberis가378균주(80.4%)로 조사되었다. 연도별 경향을 보면 2003년에는 S. iniae와 S. parauberis 분리비율이 각각 56.9%와 43.1%로 조사되었으나 이후 지속적으로 S. iniae의 분리율이 감소하고 S. parauberis 분리율이 증가하기 시작하여 2015년부터는 전체분리주가 모두 S. parauberis로 확인되었다.
S. parauberis로 동정된 균주들에 대하여 PCR을 통한 serotype 분석 결과 subserotype Ⅰa 34.9%, subserotype Ⅰb/Ⅰc 46.3%, serotype Ⅱ 18.8% 순으로 조사되었다. S. parauberis에 대한 serotype 분포변화 조사 결과에서는 2003년과 2004년에는 serotype Ⅱ가 각각 59.1%, 50.0%로 가장 많았으나, 2005년부터 2009년 사이에는 subserotypeⅠb/Ⅰc가 가장 많았으며(각각 57.6%, 86.0%, 84.6%, 57.9%, 83.3%), 2010년부터 이후에는 2015년을 제외하고는 subserotypeⅠa가 가장 높은 비율로 분리되었고, 최근 3년(2018년부터 2020년)동안을 보면 sub serotypeⅠa가 가장 많은 약 70% 내외이며, subserotypeⅠb/Ⅰc가 약 16~30%, serotype Ⅱ는 2018년에는 분리되지 않았으나 2019년과 2020년에 각각 3.4%와 16.7%로 다시 증가하는 경향을 보였다.
넙치의 연쇄구균병은 성어에 발생하여 큰 경제적 피해를 유발하므로 이에 대한 대책연구를 위해서는 지속적인 모니터링 연구가 필요하다고 생각된다.


During the period from 2003 to 2020, a total of 470 Streptococcus species were isolated from farmed flounder in Jeju. Out of 470 isolates, 92 (19.6%) isolates and 378 isolates (80.4%) were identified as Streptococcus iniae and S. parauberis, respectively by multiplex PCR assay. During that period, the percentage of S. iniae decreased from 56.9% in 2003 to 0.0% in 2020 whereas that of S. parauberis increased from 43.1% in 2003 to 100% in 2020. In the PCR assay for serotyping, the isolated S. parauberis showed 3 subserotypes, Ⅰa (34.9%), Ⅰb/Ⅰc (46.3%) and II (18.8%). In 2003 and 2004, serotype II was dominant at 59.1% and 50.0% of isolation rates, however between 2005 and 2009, subserotype Ib/Ic was dominant (57.6%, 86.0%, 84.6%, 57.9%, and 83.3%). After 2010, except for 2015, subserotype Ia was the most dominant one. In the last 3 years (2018 to 2020), subserotype Ⅰa was most abundant (70%), followed by subserotype Ⅰb/Ⅰc (16-30%). Serotype Ⅱ was not isolated in 2018, but in 2019 and 2020, it showed an increased tendency to 3.4% and 16.7%, respectively. It is believed that continuous monitoring is necessary for research on countermeasures against streptococcosis of flounder.

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4코끼리조개(Panopea japonica)에서 분리되는 비브리오속 세균의 동정

저자 : 서현준 ( Hyun-joon Seo ) , 남우화 ( U-hwa Nam ) , 김정호 ( Jeong-ho Kim )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 33권 2호 발행 연도 : 2020 페이지 : pp. 127-138 (12 pages)

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코끼리조개 성패 및 유생으로부터 잠재적 병원성 세균 분리 및 동정을 수행하였다. 분리된 균주는 분자생물학적 기법 및 생화학적 검사를 통해 동정하였으며, 명확한 동정 및 계통수 분석을 위해 16S rDNA와 하우스키핑 유전자 (pyrH, recA, rpoA)를 결합하여 분석하는 MLSA를 적용하였다. 총 141개의 균주가 분리되었으며, 건강한 성패에서는 10개, 수포 병변을 보이는 빈사 상태의 성패에서 52개, 유생에서 79개가 분리되었다. 이 중 빈사상태의 성패에서 46개, 유생에서 39개의 균주가 Vibrio 속 세균으로 동정되었으며, 나머지 균주들은 모두 일반해양세균으로 동정되었다. Vibrio 속 세균 중에서는 수포 병변을 보이는 성패에서 Vibrio splendidus가 가장 많이 분리되었으며 기존의 V. splendidus와 함께 동일한 클러스터를 형성하였다. 하지만, 인위감염실험을 실시하지 않았고 건강한 유생에서도 V. splendidus가 분리되어 성패에서 나타난 수포와 V. splendidus와의 관계는 확실하지 않으며, 차후 추가 연구가 필요할 것으로 생각된다.


We attempted to isolate and identify potentially pathogenic bacteria from geoduck clam (Panopea japonica) larvae, juvenile and adult, focusing on Vibrios. The isolates were identified by molecular approach and biochemical characterization. In particular, we applied MLSA (multilocus sequence analysis) to the isolated Vibrios for clear identification and phylogenetic relationships, by combining 16s rDNA and several houskeeping genes (pyrH, recA, rpoA). We obtained 141 isolates; 10 from healthy adults, 52 from moribund adults with blisters and 79 from larvae. 46 from the moribund adults and 39 from the larvae were identified as Vibrio species, while the rest of these samples and all the isolates from healthy adult were identified as marine general bacteria. Among Vibrio species, Vibrio splendidus was the most frequently identified from the moribund adults and clustered with the known V. splendidus in GenBank by MLSA. However, it was still unclear that V. splendidus was the cause of blisters because the artificial infection experiment was not conducted and V. splendidus was isolated also from the larvae. Further studies are necessary to clarify the etiological agent of the blisters found in geoduck clam in this study.

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5Disinfectant effect of monopersulfate (MPS) compound to white spot syndrome virus (WSSV) of shrimp

저자 : Cwr Gunasekara , Seok-ryel Kim , Lgtg Rajapaksha , Shmp Wimalasena , Hnks Pathirana , Gee-wook Shin

발행기관 : 한국어병학회 간행물 : 한국어병학회지 33권 2호 발행 연도 : 2020 페이지 : pp. 139-143 (5 pages)

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This study investigated the disinfection effect of monopersulfate (MPS) compound against white spot syndrome virus (WSSV) by bioassay using kuruma shrimp (Marsupenaeus japonicas). A WSSV stock was prepared with muscle homogenate from WSSV-infected whiteleg shrimp (Penaeus vannamei) and its lethal dose 50% endpoint (LD50) and infectious dose 50% endpoint (SID50) were respectively determined as 10-5.63 and 10-6.79 in bioassay using kuruma shrimp, followed by PCR assays. The disinfective effect of MPS compound (1.2 ppm, 2.4 ppm, 4.8 ppm) was performed by bioassay using about 10-fold higher dilution (10-4) of WSSV homogenate. The compound resulted in WSSV inactivation by a concentration-dependent manner. In addition, 4.8 ppm of MPS completely prohibited WSSV infection. To our knowledge, this study is the first report about the usefulness of MPS as a disinfectant to WSSV.

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6저염도에서 생육가능한 미생물 probiotics가 저염분 양식의 흰다리새우(Litopenaeus vannamei)의 성장 및 면역능에 미치는 영향

저자 : 배준성 ( Jun Sung Bae ) , 이채원 ( Chae Won Lee ) , 양찬영 ( Chan Yeong Yang ) , 정은하 ( Eun Ha Jeong ) , 김은진 ( Eun-jin Kim ) , 박관하 ( Kwan Ha Park )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 33권 2호 발행 연도 : 2020 페이지 : pp. 145-151 (7 pages)

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흰다리새우는 광범위한 염도(1-40 psu)에서 생존 가능하다. 하지만 염도의 변화, 특히 낮은 염도(1-5 psu)는 생존율, 비특이적 면역능 및 항병능과 같은 다양한 생리적 변화에 영향을 미칠 수 있다. 본 연구에서는 73일 동안 경구 투여 및 사육수 첨가를 동시에 수행한 뒤 성장, 비특이적 면역 및 질병 저항성을 측정하였다. 사육수의 염도는 주기적으로 소금을 첨가하여 3 psu를 유지하였다. 그 결과 73일간 사육 후 생존율이 크게 증가하였으며 (5.6 vs. 15.4%), reactive oxygen species (ROS) 생산과 phenol oxidase (PO) 활성의 유의성있는 증가가 확인되었다. 대조적으로 superoxide dismutase (SOD)활성은 영향을 받지 않았다. 새우에서 Vibrio alginolyticus의 인위 감염 시험의 결과, 프로바이오틱스 처치구에서 약간의 폐사 감소가 관찰되었다 (100 vs. 79%). 결과적으로 저염도(3 psu)에서 양식된 흰다리새우의 생산은 저염도에서 생존가능한 프로바이오틱스에 의해 증가 될 수 있을 것이다.


The whiteleg shrimp Litopenaeus vannamei can survive in a wide range of salinity (1-40 psu). However, such variation, particularly at low salinity (1-5 psu), can affect various physiological changes such as survival rates, non-specific immunity and disease resistance. In this study, growth, non-specific immunity and disease resistance were measured following simultaneous oral feeding and addition of probiotic microbes into culture water for 73 day period. The salinity of the culture water was maintained at 3 psu by periodical salt additions. The result shows that survival rate increased significantly (5.6 vs. 15.4%) after 73 day rearing. Significant increases were identified in reactive oxygen species (ROS) production and phenol oxidase (PO) activity. However, superoxide dismutase (SOD) activity was not influenced. When the shrimp was artificially challenged with Vibrio alginolyticus, slight mortality reduction was observed in the probiotics-treated group (100 vs. 79%). In conclusion, the production of cultured whiteleg shrimp at low salinity might be increased by probiotics survivable at low salinity levels.

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7뱀장어(Anguilla japonica)에서 Cytochrome P450 1 gene 클로닝 및 benzo[a]pyrene 노출에 따른 발현 분석

저자 : 조현호 ( Hyun Ho Jo ) , 김주안 ( Ju An Kim ) , 이승현 ( Seung Hyun Lee ) , 정준기 ( Joon Ki Chung )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 33권 2호 발행 연도 : 2020 페이지 : pp. 153-161 (9 pages)

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Cytochrome P450(CYP) gene is involved in the biotransformation of drugs and environmental pollutants. In this study, we analyzed the nucleotide sequence of the Anguilla japonica CYP1(AjCYP1) family gene and examined the relative expression of AjCYP1A, AjCYP1B and AjCYP1C1 in response to the exposure to environmental pollutants. After exposure to B[a]P 20mg/kg bw, the expression of AjCYP1 family gene increased over time. Among four tissues examined (liver, spleen, gill and kidney), AjCYP1 family gene was expressed significantly in the kidney. Compared with the control group, AjCYP1A was expressed about 5-fold at 48 hr, AjCYP1B about 6-fold at 24 hr, and AjCYP1C1 about 4-fold at 24 hr. However, after exposure to B[a]P 200mg/kg bw, AjCYP1A did not change in all tissues. On the other hand, AjCYP1B was expressed at about 4-fold at 24 hr in the spleen and 4-fold at 48 hr in the gill. Finally AjCYP1C1 was expressed 3.7-fold and 4.3-fold in the spleen and kidneys at 48 hr, respectively. Taken together, our results suggest that the expression of AjCYP1 gene in eel tissues might be used as a useful tool to assess the exposure to environmental pollutants in aquaculture system

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8Generation of heterologous proteins-expressing recombinant snakehead rhabdoviruses (rSHRVs) using reverse genetics

저자 : Jun Soung Kwak , Sujeong Ryu , Ki Hong Kim

발행기관 : 한국어병학회 간행물 : 한국어병학회지 33권 2호 발행 연도 : 2020 페이지 : pp. 163-169 (7 pages)

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Snakehead rhabdovirus (SHRV) is different from other fish novirhabdoviruses such as viral hemorrhagic septicemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV), and hirame rhabdovirus (HIRRV) in that it replicates at high temperatures. Therefore, the delivery of foreign proteins to fish living at high water temperature would be possible by using recombinant SHRVs. In the present study, to evaluate the possible use of SHRV as a vehicle for foreign proteins delivery, we generated a recombinant SHRV that contains an enhanced-GFP (eGFP) gene between nucleoprotein (N) and phosphoprotein (P) genes (rSHRV-A-eGFP), and another recombinant SHRV expressing two heterologous genes by inserting an eGFP gene between N and P genes, and mCherry gene between P and M genes (rSHRV-AeGFP-BmCherry). Epithelioma papulosum cyprini (EPC) cells infected with the recombinant SHRVs showed strong fluorescence(s), suggesting the possible availability of recombinant SHRVs for the development of combined vaccines by expressing multiple foreign antigens.

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9Marine birnavirus (MABV)에 대한 단클론 항체 생산

저자 : 공경희 ( Kyoung-hui Kong ) , 오명주 ( Myung-joo Oh ) , 김위식 ( Wi-sik Kim )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 33권 2호 발행 연도 : 2020 페이지 : pp. 171-175 (5 pages)

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We developed and subsequently characterized mouse monoclonal antibodies (mAbs) against marine birnavirus (MABV). Eight hybridoma clones secreting mAbs against MABV were established. All eight mAbs (8G6, 11C3, 15E3, 17H6, 32A6, 35A7, 38B5, and 47E3) were reacted with viral protein 3 of MABV in MABV-infected CHSE-214, whereas, no reactivity was observed in normal CHSE-214 by western blot analysis. Moreover, these eight mAbs were strongly reacted with MABV, and no cross-reactivity has been observed against other five fish viruses (hirame rhabdovirus, infectious hematopoietic necrosis virus, nervous necrosis virus, spring viraemia of carp virus, and viral hemorrhagic septicemia virus), although five mAb (11C3, 15E3, 17H6, 32A6, and 38B5) reacted with both MABV and infectious pancreatic necrosis virus by enzyme linked immunosorbent assay (ELISA). These results indicate that the mAbs can be of value in MABV detection.

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10진피(Citrus unshiu Peel)추출물이 첨가된 사료의 급이가 넙치의 성장률 및 항스쿠티카충 효과에 미치는 영향

저자 : 방석진 ( Seok Jin Bang ) , 최재혁 ( Jae Hyeok Choi ) , 정상목 ( Sang Mok Jung ) , 강인성 ( In Sung Kang ) , 이찬흔 ( Chan Heun Lee ) , 박관하 ( Kwan Ha Park ) , 최상훈 ( Sang Hoon Choi )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 33권 2호 발행 연도 : 2020 페이지 : pp. 177-184 (8 pages)

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In an attempt to find a feed additive showing an anti-scuticociliate effect, extracts from Citrus unshiu Peel were tested against virulent scuticociliate infection. The most effective anti-scuticociliate killing activity in vitro was observed in the extract squeezed from homogenizing water-soaked dried tangerine peel (DTP). In addition, we have investigated the effect of DTP as a feed additive on growth rate and anti-parasitic activity of olivaceus flounder. DTP extract added diets (0.1, 0.5, 1, and 5%/feed weight) were fed to flounder for 7 days for checking a growth rate and 14 days for a challenging test. As a result, the feed conversion rate was significantly improved only in 1% DPT extract group compared to the control and 0.5% DTP extract fed group showed 100% of survival rate in the challenge test, all of which indicating that DTP extract would be a potential feed additive against scuticociliatosis.

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1연안해역에서 석유오염물질의 세균학적 분해에 관한 연구

(2006)홍길동 외 1명심리학41회 피인용

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2미국의 비트코인 규제

(2006)홍길동41회 피인용

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해당 간행물 관심 구독기관

선문대학교 부경대학교 전남대학교 조선대학교 경상대학교
 90
 72
 47
 16
 15
  • 1 선문대학교 (90건)
  • 2 부경대학교 (72건)
  • 3 전남대학교 (47건)
  • 4 조선대학교 (16건)
  • 5 경상대학교 (15건)
  • 6 군산대학교 (9건)
  • 7 해양수산부 (6건)
  • 8 제주대학교 (5건)
  • 9 동아대학교 (4건)
  • 10 충북보건과학대학교 (3건)

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