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Oral Biology Research (OBR) update

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수록범위 : 1권0호(1978)~45권2호(2021) |수록논문 수 : 1,453
Oral Biology Research (OBR)
45권2호(2021년 06월) 수록논문
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KCI등재

1Effect of erbium-doped yttrium-aluminum-garnet laser on surface characteristics and biocompatibility of resorbable blast media titanium

저자 : So-won Uhm , In-seok Lee , Bo-hyun Kim , Young-joon Kim

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 45권 2호 발행 연도 : 2021 페이지 : pp. 49-58 (10 pages)

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Er:YAG laser irradiation can modify the surface characteristics of titanium surface. This study aimed to evaluate the influence of Er:YAG laser irradiation on mechanical, chemical, and biological characteristics of resorbable blast media (RBM) titanium surfaces. RBM disks were divided into 4 groups. Control groups were nonirradiated RBM disks. Laser irradiated groups were divided into the RBM-50, RBM-100, and RBM-250 group which irradiated Er:YAG laser at energy level 50, 100, and 250 mJ/pulse, respectively. In scanning electron microscopy (SEM) images, whereas the RBM-50 group showed slightly melted surface area, RBM-100 and RBM- 250 groups showed broken areas caused by Er:YAG laser. Laser irradiated groups had significantly higher roughness than the control group. The roughness of the RBM-50 group was comparable with the RBM-100 group and significantly lower than the RBM-250 group (p<0.05). In X-ray diffractometer analysis of the laser irradiated group, Ti (002) (rutile) proportion to Ti (101) (anatase) was decreased according to energy level. In X-ray photoelectron spectroscopy analysis, RBM-50 and RBM-100 groups show the crystal change from acidic Ti-OH to basic Ti-OH. MC3T3-E1 cells of all groups adhered and proliferated well on the surfaces and their morphology was not different between groups in SEM images. The alkaline phosphatase activity levels of RBM-50 and RBM-100 groups showed higher than those of the control group (p<0.05). Therefore, within the limitations of this study, Er:YAG laser irradiation at low energy levels can modify mechanical and chemical characteristics of the RBM surface, leading to improve the biological responses of osteoblastic cells on the surface.

KCI등재

2Students' perceptions of the impact of video lectures with embedded quiz questions on e-learning in dentistry

저자 : Won-pyo Lee , Joo-hun Song

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 45권 2호 발행 연도 : 2021 페이지 : pp. 59-65 (7 pages)

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During the COVID-19 pandemic, e-learning has become more prevalent in dental education. In this study, we investigated the effectiveness of video lectures with embedded quiz questions. A questionnaire survey was conducted on the preferences of each educational method for dental students (n=70) who took all three lecture types (offline lecture, Group 1; video lecture without embedded quiz, Group 2; and video lecture with embedded quiz, Group 3). In terms of other behaviors that imply the nonacademicrelated activities, Group 2 was significantly the highest, whereas in terms of lecture flow, it was the lowest. In terms of learning motivation and learning confidence, Group 3 lecture type was significantly preferred. In terms of communication with professor and communication with students, Group 1 was the most preferred, but there was no significant difference compared with Group 3. In terms of overall lecture satisfaction, Group 3 lecture type was significantly the highest. The e-learning method using video lectures with embedded quiz questions could be an effective alternative to traditional offline lectures in dental education.

KCI등재

3Characterization of the hemin-binding property of Porphyromonas endodontalis

저자 : Eun Jeong Kim , Si Young Lee

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 45권 2호 발행 연도 : 2021 페이지 : pp. 66-74 (9 pages)

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Porphyromonas endodontalis, found in the root canal of teeth, requires iron for growth. However, the mechanism of iron uptake in P. endodontalis remains unclear. The ability of bacteria to utilize heme compounds to acquire iron for growth has been reported in some pathogenic bacteria. In the present study, we analyzed the ability of P. endodontalis to obtain iron from heme compounds. Further, we investigated the hemin-binding characteristics of P. endodontalis and the relationship between hemin binding and Congo red binding. To confirm the bacterial growth in hemin-supplemented medium, iron was removed from the medium with an ironchelator, and hemin was supplemented to an iron-free medium. The hemin-binding characteristics of P. endodontalis were analyzed by incubating bacteria with hemin and measuring the optical density of the supernatant obtained via centrifugation, using hemin concentration standard curve. Although growth of P. endodontalis was not observed in the iron-depleted medium, it was observed in a hemin-supplemented medium. Further, hemin binding was dependent on the concentrations of hemin and bacteria. Hemin binding proceeded quickly in P. endodontalis, and the incubation temperature had no effect on this binding. Similar to hemin binding, Congo red binding of P. endodontalis was dependent on Congo red and bacterial concentrations. In addition, Congo red binding of P. endodontalis was inhibited by hemin prebinding. Hemin-agarose beads and SDS-PAGE were used to identify a 40-kDa protein that could be involved in hemin binding. The results showed that P. endodontalis could bind to and use hemin to obtain the iron required for growth.

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4Genetic diversity of the tet(M) gene in tetracycline-resistant oral streptococci

저자 : Dasul Jeong , Si Young Lee

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 45권 2호 발행 연도 : 2021 페이지 : pp. 75-81 (7 pages)

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Tetracycline resistance occurs at a high frequency among clinical isolates of both gram-positive and gram-negative bacteria. The mechanism and genetics of tetracycline resistance have not been extensively studied in streptococci, although the overwhelming majority of clinical isolates are tetracycline resistant. tet (M) is the most common tetracycline-resistance gene in streptococci. The aim of this study was to examine the genetic diversity of tet (M) genes in tetracycline-resistant oral streptococci from dental plaque. Streptococci were isolated from supragingival plaque samples of healthy persons. The isolates were then identified at the species level, and the minimum inhibitory concentration (MIC) of tetracycline was determined. Genomic DNA was extracted from tetracyclineresistant isolates and tet (M) was amplified using polymerase chain reaction with tet (M)-specific primers. The polymerase chain reaction products were cloned, DNA sequencing was performed, and the sequences were compared using an alignment program. The estimated nucleotide divergence between different tet (M) alleles ranged from 0.00% to 6.07% among oral streptococci. The tet(M) genes from oral streptococci consisted of regions similar in sequence, interspersed with regions that differed at some nucleotide sites, revealing a mosaic structure. The percent nucleotide divergence of tet(M) was unrelated to the MIC values of tetracycline for oral streptococci, and bacterial strains in the same streptococcal species showed different heterogeneity in tet(M). The divergences of tet(M) nucleotide sequences among oral streptococci were comparable with those of other bacterial genera. Our findings may provide basic information about the transposition processes associated with tet(M) in oral streptococci.

KCI등재

5Identification and characterization of a hemagglutinin gene from Prevotella intermedia

저자 : Seok-woo Lee , Masaaki Okamoto , Kai P. Leung

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 45권 2호 발행 연도 : 2021 페이지 : pp. 82-89 (8 pages)

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In this study, we describe the cloning of a gene encoding hemagglutinin from Prevotella intermedia to characterize hemagglutinin function and elucidate its possible role in the infectious process mediated by the organism. We screened genomic libraries created in Escherichia coli JM109, using antibodies against P . intermedia , and the positive clones were subsequently tested for their hemagglutinating activity, using rabbit red blood cells. We isolated a clone of strain 0543 in which, a 3.6-kb P. intermedia chromosomal DNA fragment was inserted into the pUC18 plasmid. Our deletion analysis-based subcloning procedures identified a hemagglutinin gene―designated phg. The open-reading frame of phg encoded a 35-kDa protein, and the expression of a recombinant protein of the predicted size was observed in a fusion protein expression system. While the DNA analysis of phg revealed no homology to known DNA sequences, the putative promoter, ribosome binding, and transcriptional termination sequence were found in the flanking DNA regions. In the N-terminus of the deduced amino acid sequence, we found a consensus leader peptide sequence for secretory proteins. These findings indicate that hemagglutinin might be expressed on the surface of P. intermedia to mediate its function.

KCI등재

6Controlled releasing properties of gelatin nanofabric device containing chlorhexidine

저자 : Dae-ung Park , Heung-sik Um , Beom-seok Chang , Si-young Lee , Ki-yeon Yoo , Won-youl Choi , Jae-kwan Lee

발행기관 : 조선대학교 치의학연구원 간행물 : Oral Biology Research (OBR) 45권 2호 발행 연도 : 2021 페이지 : pp. 90-98 (9 pages)

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We aimed to evaluate the degradation and antimicrobial activity of novel chlorhexidine-containing gelatin nanofabric devices and their cytotoxicity in animals. The electro-spun device with a size of 3.4.0.4 mm3 was prepared by entrapping chlorhexidine gluconate (CHX) in a gelatin matrix. The devices were divided into three groups based on the CHX percentage (G1: 46%, G2: 50%, and G3: 54%), and the commercial product, PerioChip (CP), was used for the control group. We used an in vitro test for the degradation and antibacterial activity and investigated cytotoxicity using an in vivo test. Artificial saliva was used for the degradation test of chips and blood agar plates seeded with the oral bacteria Streptococcus sanguinis to address antibacterial activity. Furthermore, the devices were inserted between the skin and muscle of rat abdomens to evaluate infection and inflammatory reactions. We detected no obvious differences in the antibacterial or degradation test results between the test and control groups. In the histological analysis, the scaffold without CHX showed no signs of infection or inflammatory reaction. Both CHX-containing groups exhibited inflammation and necrosis in the muscle and skin, although the reaction in the test group was milder. In the degradation tests, antibacterial tests, and cytotoxic reactions in animal experiments, the electro-spun gelatin-based device showed similar results to those of the commercial device in the control group. Further studies on the quantitative analysis and clinical outcome evaluation of the electro-spun gelatin-based device in humans are necessary.

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