CKD-602 exerts its antitumor effect via inhibition of topoisomerase I in cancer cells. Multicellularin vitro 3-dimensional models which closely rep-resent tumor conditions in vivo. In order to investigate the potential of CKD-602 against human colorectal tumors,we evaluated the anti-proliferative activity and penetration ability of CKD-602 in MCS and MCL cultures ofDLD-1 human colorectal cancer cels, respectively. The maximum effects (Emax) induced by CKD-602 were sig-nificantly lower in MCS compared to monolayers (48% vs 92%). With prolonged drug exposure, the IC50’s ofCKD-602 decreased to 23.5±1.0nM in monolayers after 24 h exposure and 42.3±1.7 nM in MCS after 6 days,respectively. However, no further increase in effect was observed for exposure time longer than growth doublingtime (Td) in both cultures. Activity of CKD-602 was significantly reduced after penetration through MCL andalso with cel-fre insert membrane. In conclusion, CKD-602 showed significantly decreased anti-proliferativeactivity in 3D cultures (MCS) of human colorectal cancer cels. Tumor penetration of CKD-602 could not bedetermined due to loss of activity after penetration through cel fre insert membrane, which warrants further eval-uation using a modified model.Keywords . CKD-602, Belotecan, Pharmacodynamics, Multicellular spheroid, Multicellular layer, solidtumor, penetration

<Abstract> Song Shi-Yeol's Theory of the Genealogy of the Confucianism and Modifications to the Policies of the National Confucian Shrine Woo, Kyung-sup* King Hyojong's reign, Song Shi-Yeol engaged in further studies and teachings of Chu Hsi. Song supposed himself and his fellow Seoin/西人 Sanrim/山林 members to the successor of Chu Hsi, and reviewed the studies of former masters of him such as Lee Ih/李珥, Kim Jang Saeng/金長生 and Jo Heon/趙憲, with the Cuase of Justice theory(義理論). Song's Cause of Justice theory triggered the effort of reviewing the past Confucian figures during the reign of King Hyeonjong. He suggested a new way of evaluating someone or something based upon the character(人品) and the level of engagement in Confucian ideologies. In the process, a new genealogy of historical figures was established with Gija/箕子, Jeong Mong-Ju/鄭夢周, Jo Gwang-Jo/趙光祖, and Lee Ih/李珥 in line. During the reign of King Hyeonjong, Song tried to consolidate the legitimacy factor of the Neo-Cofucianism by bringing modifications to the National Confucian Shrine policies and practices(文廟祀典), and to establish the status of Lee Ih and Kim Jang Saeng as the true successors of Chu Hsi's studies and philosophy.