고추 疫病菌(P. capsici)에 優秀한 抗菌活性을 가진 3가지의 抗菌物質을 Pseudomonas sp. A-183 培養液으로 부터 分離 精製하였다. 分離된 3가지의 抗菌物質에 대한 分子構造式을 究明하기 위하여 UV, IR, ¹H NMR, ^(13)C NMR Spectroscopy法으로 해석하였다. A와 B物質은 Molish 및 Anthrone反應에 陽性으로, Fehling反應에는 陰性으로 나타났으므로 nonreducing sugar構造를 含有하고 있는 것으로 판단되었다. C物質은 위의 試藥에서 呈色反應을 나타내지 않고 단지 UV吸收만을 나타내었다. Mass, NMR 및 IR 分光分析에 의해 A와 B物質은 糖으로는 deoxyhexose인 rhamnose를 포함하고 지방산으로는 β-hydroxydecanoic acid를 포함하였다. 또 이들 사이에는 O-glycoside結合이 形成되어 있었으며 A와 B物質 사이에는 糖 한分子의 差異가 나타났다. 綜合的으로 A物質은 glycolipid B인 3-hydroxy-(3-hydroxydecanoyl) -decanoic acid 3-O-α-L-rhamnopyranosyl(1→2)rhamnopyranoside, B物質은 glycolipid A인 3-hydroxy- (3`-hydroxydecanoyl) decanoic acid 3-O-α-L-rhamnopyranoside로 同定되었으며, C物質은 phenazine으로 確認되었다.

This study was carried out to identify the three antifungal substances isolated from the culture medium of Pseudomonas sp. A-183 which is antagonistic against Phytophthora capsici. The substance A and B showed positive reactions at the Molish test and Anthrone test, but negative one at the Fehling test, strongly suggesting that both substance A and B had nonreducing sugar frameworks. The substance C only exhibited the phenomenon of the UV induced fluorescence. From the qualitative analysis with the spectroscopic techniques such as UV, Mass, IR and NMR, the substance A and B were known to be composed to sugar and fatty acid, and showed a base peak of 171(m/e). It was identified that substance A was (2-O-L-rhamnosyl-α-L-rhamnosyl-β-hydroxydecanoyl-β-hydroxy decanoic acid) and the substance B was (α-L-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoic acid). The substance C was identified as a phenazine from the results of qualitative analysis with the spectroscopic techniques such as UV, Mass, IR and NMR.