農畜産 廢棄物의 嫌氣的 處理工程에 적용하기 위하여 亞寒帶 地域으로 부터 分離한 clostridia의 生化學的 特性을 調査한 結果는 다음과 같았다. 1. Cellulose 배지로 低溫에 집적培養한 결과 CH₄생성이 旺盛 하였던 늪지 堆積物(Canada, 56.9。N), 호수堆積物 Ⅳ(Canada, 55.0。N) 그리고 갯벌흙 Ⅱ(Korea, 37.0。N)에서 酸生成能이 우수하고 동시에 메탄을 생성하는 Clostridia, 즉 Clostridium botulinum SRC-64, Clostridium scatologens SRC-91 및 Clostridium tyrobutyricum SRC-100 을 각각 分離하였다. 分離된 clostridia 3菌株의 最適 生育溫度는 37℃이었고 最低 生育溫度는 10℃ 정도 이었으며, 그리고 이들 菌株는 특이적 메탄 生成菌의 組酵素인 F_(420)을 함유 하였다. 2. 포도당의 嫌氣分解 生成物로서 SRC-64 는 H₂, acetic, isovaleric, caproic acid 를 生成하였으며, SRC-91 은 H₂, propionic, butyric, valeric, caproic acid 를, SRC-100 은 acetic 과 Propionic acid 를 주로 生成 하였다. 또한 3 菌株 모두 13℃, 2 日 培養條件에서 2.6-8.68 n moles/㎖ 範圍의 CH₄ 生成能力을 나타냈다.

This study was conducted to investigate the biochemical properties of isolated bacteria, low temperature tolerant methane-producing clostridia which were selected for using them as inoculum to anaerobic fermentation of agricultural and livestock wastes at low temperature. The results were; 1. Low temperature tolerant methane-producing clostridia were isolated from the samples which showed the high methanogenesis rate by enrichment culture at low temperature in cellulose medium. These clostridia, Clostridium botulinum SRC-64, Clostridium scatologens SRC-91 and Clostridium tyrobutyricum SRC-100, were isolated from swampy sediment at latitude 56.9。N, lake sediment IV at latitude 55.0。N, and tidal land soil II at latitude 37.0。N, respectively. The optimum growth temperature for these isolates was 37℃ and the minimum, around 10℃. They all had detectable amount of F_(420), specific coenzyme of methanogens. 2. As anaerobic fermentation products of glucose SRC-64 produced H₂, acetic, isovaleric and caproic acid, SRC-91 produced H₂, propionic, butyric, valeric, and caproic acid, and SRC-100 produced only acetic and propionic acid. The isolates were produced CH₂ ranged from 2.6 to 8.68 n moles/㎖ for 2 days at 13℃.