Pentachlorophenol(PCP)가 大豆 및 벼 縣濁培養과 과 수도체 중에서 水溶性成分으로 轉換되는 것을 경시적으로 조사하여 다음과 같은 결과들 얻었다. 1. 현탁배양에서 처리한 PCP의 50%가 수용성 대사물로의 전환되는 시간은 대두의 경우 4시간, 벼는 48시간 이내였다. 수용성 대사물의 생성속도와 양은 PCP의 cell 생육 저해 정도와 밀접한 관계가 있었다. 2. 식물 cell에서 PCP의 主 代謝過程은 生育時期 및 藥劑 處理時間을 달리한 전환시험에서 얻은 수용성 대사물 및 glucose cunjugates 함량변화 조사와 conjugates가 가수분해되는 성질로부터 glucose conjugates(β-anomers)형성 反應임을 알 수 있었다. 3. Cell에서 glucose conjugates는 藥劑處理 초기 및 대수성장기 이전에 생성 속도가 매우 빨라 전체 수용성 대사물의 70% 이상이었으며, 점차 비가수분해성 極性成分으로 전환되었고, 일부는 培養液으로 分泌되었다.

A metabolic study has been conducted to investigate the conversion of pentachlorophenol(PCP) to water soluble metabolites in soybean and rice cell suspension cultures as well as in intact rice plants. PCP in plant cells was found to be exclusively transformed into water soluble metabolites. The relative rate of the metabolic conversion of PCP in decreasing order was soybean cultures > rice cultures > rice plants. Also observed was that, the older the cultures grown, the lower the conversion rate was. Primary water soluble metabolites isolated from both the 5 day old soybean and 8 day old rice cells were specifically hydrolyzed only by β-glucosidic linkage specific glucosidase, suggesting that the metabolites are β-glucose conjugates. The amount of glucose conjugates was increased with increasing time of incubation of PCP up to 24 hr in both soybean and rice cultures; Thereafter, it was decreased progressively. Most of the glucose conjugates were further metabolized to more polar conjugates in cells, but a portion of them was excreted into the culture medium.