The denitrifying potential and various environmental factors were investigated and the influence of environmental factors on the distribution of denitrifying bacteria and potential was analyzed through stepwise multiple regression in the sediment of lake Daeho. The N_2O production was measured with gas chromatography at in situ temperature by acetylene blockage method and the N_2O production rates were 0∼9.18㎍-N/g-dw/day. The genera that showed denitrification potential were identified as Pseudomonas, Corynebacterium, Flavobacterium, etc. It was shown through statistical analysis that the environmental factors affecting the variation of denitrifying bacteria and potential differed with stations. NO_3 was used as a terminal electron acceptor, however terminal electron acceptor could be changed by environmental factors.