In addition to sterols, some fatty acids were analyzed for the purpose of estimating the fecal pollution in Joongryang and Wangsug stream as reported previously. Palmitic, myristic and stearic acid were predominant but linoleic and lonolenic acid were detected with a small amounts. It seemed to have been originated mainly from the sewage water for the kitchen works. As the results, the contents of fatty acids in mud were much more abundant the those in water as the same case of sterols.