To study the difference of aqueous soluble muscle protein pattern of fresh-water fishes in geographical populations the method of disc-electrophoresis using polyacrylamide was adapted. 1) The number of bands of aqueous soluble muscle protein were 10 in Zacco platypus, 7 in Zacco temmincki, 6 in Pungtungia herzi, 7 in Pseudopungtungia tenuiscorpus, 5 in Rhodus ocellatus, 8 in Coreoleuciscus splendidus, 9 in Microphysogobio longidorsalis. 2) The number of bands, degree of stain, mobility and rate of contents were different in interspecies. 3) No electrophoretic pattern was observed different among the four locality.