土壤腐植酸의 形態(Rp, B, A, P)別 腐植酸과 fulv 酸中 amino酸의 含量과 組成을 규명코자 腐植酸과 fulv酸을 各各 分離 精製하여 分析해 얻은 結果를 要約하면 다음과 같다. 1. 腐植의 組成 全腐植量(H_T), 腐植酸量(a), fulv 酸量(b), 色調係數(ΔlogK)는 Rp→B→A→P型으로 갈수록 적어졌고 全窒素 全炭素도 같은 경향이었다. 2. 腐植酸中 amino酸의 含量 및 組成 가. 腐植酸의 形態別 全 amino酸의 含量은 針葉樹林土壤에서는 Rp$gt;B$gt;A$gt;P型의 順이었으나 闊葉樹林土壤에서는 P$gt;A$gt;Rp$gt;B型의 順이었다. 全 amino酸과 腐植組成과의 關係에서 針葉樹林土壤에서 全炭素 ΔlogK와는 正의 相關이 있었고 C/N率과는 負의 相關이 있었다. 闊葉樹林土壤에서는 有意相關이 없었다. 나. 形態間 酸性, 中性, 鹽基性 amino酸의 含量比는 針葉樹林土壤에서 酸性 amino酸은 P$gt;Rp$gt;B$gt;A型의 順, 中性 amino酸은 Rp$gt;B$gt;A$gt;P型의 順, 鹽基性amino酸은 B$gt;A$gt;Rp$gt;P型의 順이었다. 含量은 中性$gt;酸性$gt;鹽基成 amino酸의 順이었다. 闊葉樹林土壤에서는 酸性 amino酸 A$gt;P$gt;B$gt;Rp型의 順, 中性 amino酸은 P$gt;Rp$gt;A$gt;B型의 順, 鹽基性 amino酸은 P$gt;A$gt;B$gt;Rp型으로 근소한 차이였다. 다. 形態別 各 amino酸의 含量에서는 전체적으로 aspartic acid, glycine, glutamic acid가 많았고 histidine, tyrosine, methionine이 적었다. 3. Fulv 酸中 amino酸의 含量 및 組成 가. 形態別 全 amino酸의 含量은 두 土壤 共히 Rp$gt;B$gt;P$gt;A型의 順이었다. 針葉樹林土壤에서 全amino酸과 全炭素, ΔlogK와 正의 相關이 있었고 闊葉樹林土壤에서는 全窒素, 全腐植量(H_T), 腐植酸量(a), 色調係數(ΔlogK)와는 正의 相關이 있었고 C/N率과는 負의 相關이 있었다. 나. 形態間 酸性, 中性, 鹽基性 amino酸의 含量比는 두 土壤 共히 Rp$gt;B$gt;P$gt;A型의 順이었다. 다. 形態間 各amino酸의 含量을 比較할때 全體的으로 glycine, aspartic acid, alanine이 많았고 tyrosine, methionine은 적었고 arginine은 거의 측정되지 않았다.

Contents and distribution of amino acids in the humic acid and fulvic acid fractions of different types (Rp, B, A, P) were investigated. Extracted humic and fulvic acids were purified and analyzed. The results are summarized as the following: (1) Composition of Humus The total humus (H_T), amount of humic acid (a), amount of fulvic acid (b), and Δ log K all decrease in the order of Rp $gt; B $gt; A $gt; P type. The same trend was observed in the total nitrogen and carbon. (2) Contents and composition of amino acids in humic acids. 1) The total amounts of amino acids in the humic acid fraction of different types were in the following order for soils under coniferous forest trees: Rp $gt; B $gt; A $gt; P type, but for soils under deciduous forest trees the order was P $gt; A $gt; Rp $gt; B type. There were positive correlationships between total amino acids and total carbon and Δ log K for humic acids from soils under coniferous forest trees, but a negative correlationship was existed. between total amino acids and C/N ratios. No significant correlation was found for samples taken from soils under deciduous forest trees. 2) The ratios of one group of amino acids to the others were compared, The ratios of acidic amino acids were in the order of P $gt; Rp $gt; B $gt; A type, those of neutral amino acids followed the order of Rp $gt; B $gt; A $gt; P type and those of the basic amino acids were in the order of B $gt; A $gt; Rp $gt; P type for soils under coniferous forest trees. Contents of total amino acids were in the order of the neutral $gt; the acidic $gt; the basic amino acids. For the soils under deciduous forest trees the order of the ratio was different. Acidic amino acids followed the order of A $gt; P $gt; B $gt; Rp type, neutral ones followed the order of P $gt; Rp $gt; A $gt; B type, and the basic amino acids did the order of P ≥ A $gt; B ≥ Rp type where the difference was very small. 3) In general aspartic aicd, glycine and glutamic acid were the major components in all samples. Histidine, tyrosine and methionine belonged to the group contained in a small amount. (3) Contents and composition of amino acids in fulvic acids. 1) The total amounts of amino acids of different types of fulvic acids were in the order of Rp $gt; B $gt; P $gt; A type regardless of origin of samples. There were positive correlationships observed between the total amino acids and total carbon and Δ logk for soils under coniferous forest trees. For soils under deciduous forest trees, positive correlationships were observed among total amino acids, total nitrogen, total humus (H_T), total humic acid (a), and Δ log k, but a negative correlationship existed between total amino acids and C/N ratio. 2) The ratio among acidic amino acids, neutral amino acids and basic amino acids of different types were Rp $gt; B $gt; P $gt; A type. In this respect there was no difference between the two soils.