A micropropagation system for black Locust(Rohinia pseudoacacia) was established by using shoots and pin-punctured leaves of in vitro germinated seedlings. The greatest number of shoots (an average of 10.5 shoots) was obtained when shoot tips were cultured on MS medium supplemented with 1.0 ㎎/ℓ BAP and 0.01 ㎎/ℓ NAA. When pin-punctured leaf explants were cultured on the same medium, mean number of 13.E shoots were produced. Shoot growth was accelerated by adding 50 ㎎/ℓ of silver nitrate (AgNO₃), an anti-ethylere compound to the culture medium. Each shoot was excised from the mass and transferred onto half strength MS medium for rooting. Zygotic embryos at different developmental stages were cultured on LS medium supplemented with various growth regulators to induce somatic embryos. When cultured on LS medium with 1.0 ㎎/ℓ 2,4-D. 14.3% of the zygotic embryos induced somatic embryos. Upon transfer onto the basal medium, somatic embryos sporadically converted into plantlets.