The axillary buds of 15-year-old Tilia amurensis were cultured on Saito and Ide (IS), Murashige and Skoog (MS) media and woody plant medium (R`PM) to establish an effective micropropagation method. Five levels of 6-benzylaminopurine (BAP) were tested. On IS medium and WPM addition of 1.0㎎/ℓ BAP enhanced shoot development and shoot elongation, whereas addition of 0.5㎎/ℓ BAP was effective on MS medium. A better results were obtained from V`PM with 1.0㎎/ℓ BAP and MS with 0.1㎎/ℓ BAP. Developed shoots were subcultured on each basal media but with 0.2㎎/ℓ BAP, Multiple shoots were almost doubled in a month. Root formation could be enhanced at higher concentration of indole-3-butyric acid (IBA). Better rooting rate (83.3%) was achieved on a half-strength MS medium with 3.0 ㎎/ℓ IBA. Regenerated plantlets were successfully transferred to soil. To investigate the clonal variation in shoot development and shoot elongation by axillary bud culturing, seven plus tree clones were tested, Clonal variation in tissue culturability among plus trees was recognized by the Duncan`s multiple range test at the 5% level. Kang Won No. 12 showed the best response on WPM with 1.0㎎/ℓ BAP.