For the development of the vegetative propagation by cuttings with superior phenotypes of Quercus acutissima, the effect of media containing various rooting substances(IBA, NAA, IAA, Sucrose, Captan and Talc.), the difference between sprout and normal branches of seedling, the position effect of cutting, seasonal variation, the difference between out-door seedling and greenhouse seedling, ortet age effect, upon the rootability and the development of root system using 1 to 3-year-old seedlings have been investigated. The obtained results were as follows 1. Survival., rooting, primary root number and primary root length of cuttings showed significant differences among the media containing rooting substances. 2. Sprout showed better rooting ability than the normal t~ranch of seedling and the number of the primary roots was abundant, too. 3. Rooting eras better on V/PL and P/V media bed than any other tested ones when media without growth substances were experimented while callus ball formation was vigorous under the part of cuttings on the medium containing perlite. 4. Basal part had slightly better rooting ability than top part with apical bud for cutting`s position. 5. Rootability was varied greatly by seasons of cutting collection. The best rooting was observed in the mid-June with semi-hard wood, after first growing season. 6. Greenhouse seedlings hadmore numbers of the primary root and rooted more readily than nurserybed seedlings. 7. The most significant difference in rooting ability was observed between the ortet ages, 58.3% and 84.4% for 1 and 3 year orter, respectively.