This study was carried out within the greenhouse of the Forestry Nursery of Chonnam National University from April, 1984 to September, 1985, being different from inoculation of P. tinctorius intervals and amount of fertilizer application with acorns collected in the fall from six different trees of Quercus acutissima growing natively at Bogil-do, Chonnam Province. These experiments researched and investigated centering on the growth volume, ectomycorrhizal morphology and anatomic observation .and nutritive absorption of Quercus acutissirna seedlings. The results are summarized as follows. 1. The ectomycorrhizal fungus, P. tinctorius (Pt) increased growth of the container-grown oak seedlings. Of three treatments, Pt inoculation stimulated remarkably primary lateral roots, shoot growth, and root collar diameter and leaf area development, compared with either noninoculated or natural soil treatment. 2. Pt-inoculated seedlings produced ectomycorrhizal by 71.1 percent, and ectomycorrhizal roots developed more abundantly in the middle portion of the container than in the top or bottom portions. 3. The development shapes of ectomycorrhizal root tips after inoculation Pt on Quercus acutissima seedlings were monopodial type, fernlike type and clusterlike type. Besides, ectomycorrhizal without root hairs were well developed in fungus mantles which surround feeder roots and they formed distinctively Hartig nets in the intercelluar of cortical zone. 4. The results of the end of June, 1985 : 1) ectomycorrhizal development was variable due to the intervals and amount of fertilizer application, When nitrogen fertilizer of 500㎍/㎖ and 750㎍/㎖ was given after Pt inoculations by every 2wk-intervals, a lot of fernlike types and clusterlike types were formed, whereas monopodial types were done in on-fertilizer treatment plat. When nitrogen fertilizer by every 5wk-intervals after Pt inoculations made, 750㎍/㎖ treatment plot obtained the best results in mycorrhizal formation. 2) Non-nitrogen fertilizer caused the development of short root to be reduced., so that mycorrhizal infection did not enough to reach the upper portion of feeder roots and the length of mycorrhizae was short. Surface of feeder roots revealed sometimes brown color. 5. The results of the end of July, 1985 : fertilizer of 250㎍/㎖ and 500㎍/㎖, after Pt inoculation, by every 2wk-intervals caused three types of mycorrhizae to be evenly spread and only 750㎍/㎖ fertilizing plot formed a lot of monopodial types. The fernlike and clusterlike types were, however, mainly distributed in 750㎍/㎖ fertilizing plot by every 5wk-intervals. The development of hyphae and Rhizomorph were very excellent . 6. The results of the end of August, 1985 : high temperature kept in general mycorrhizal formation ratio from well being done in every treatment plot land reduced it, whereas lots of clusterlike types were formed in treatment plots of high fertilizer concentrations. 7. The results of the end of September, 1985: fertilizer of 500㎍/㎖, after Pt inoculation by every 2wk-intervals, formed a lot of fernlike and clusterlike types, while clusterlike types increased in 750㎍/㎖ treatment plot. But it came out in evidence that all the three types were poor in non-fertilizer plot, on the other hand fernlike and clusterlike types were formed in a large quantity, when 700㎍/㎖ was fertilized by every 5wk-intervals. 8. The relationships were established between seedling height and leaf dry weight, and between stem dry weight and root dry weight. T/R ratio, anal total dry weight investigated as the index of seedling growth. The significant relation of a positive correlation was rendered between root dry weight and leaf dry weight, whereas the significant relation of a negative correlation was recognized between total dry weight and T/R ratio. 9. According to the growth characteristics of seedling height, root collar diameter seedling volume and total seedling dry weight, a significant differenc