닫기
3.17.173.208
3.17.173.208
close menu
}
SCIE SCOPUS
소 수정란의 세포주기 조절에 의한 핵이식에 관한 연구
Nuclear Transplantation of Bovine Embryos by Cell Cycle Stage Synchronization
정희태(H . T . Cheong), 임석기(S . K . Im), 이준희(J . H . Lee), 박춘근(C . K . Park), 양부근(B . K . Yang), 김정익(C . I . Kim)
UCI I410-ECN-0102-2008-520-002111458
* 발행 기관의 요청으로 무료로 이용 가능한 자료입니다.

This study was conducted to examine the possibility of cell cycle synchronized nuclear transfer and the effect of the early cell cycle stage of donor embryo on the development of nuclear transplant bovine embryos. Donor embryos were treated with nocodazole, then monitored their nuclear phase and cleavage after release from the nocodazole treatment. Blastomeres cleaved within 3 hr and noncleaved ones were transferred into enucleated oocytes. Some nuclear transplant embryos were fixed to investigate their chromatin structures. Comparatively high proportions(56.0-58.6%) of blastomeres arrested at mitotic phase(M-phase) were obtained following treatments with 0.5∼1.0㎍/㎖ of nocodazole for 16 hr. Cleavage rates of blastomeres within 3 hr after release from nocodazole treatment were average 64%(188/294). After nuclear transfer, 13%(11/85) of embryos were developed to blastocysts following transplantation of blastomeres within 1.5 hr post cleavage(hpc), but 7%(5/74) and 5%(3/63) were developed to blastocysts following transplantation of blastomeres at 3 hpc and noncleaved ones. About 40% of 1.5 hpc nuclear transplant embryos have a single chromatin clump, whereas only 25.0% of 3.0 hpc nuclear transplant eanbryos have a single chromatin clump and the rest have 2 or multi chromatin clumps. From the result of this study, it was suggested that the developmental potential of nuclear transfer bovine embryos may be enhanced with transfer of early cell cycle stage blastomeres immediately after cleavage. It was also suggested that the cell cycle synchronization of bovine embryonic cells at GI phase is very difficult.

[자료제공 : 네이버학술정보]
×