In study for identification the best combination of restriction enzymes(Hae III, Hind III, Hinf I, BamH I, EcoR I) and DNA probes [M13, YNZ22, Mo-I, α-globin, 33.15, (GT)n] for DNA fingerprinting of Korean Cattle (Hanwoo), it was found that M13 and YNZ22 probes made clear polymotphic bands, restriction enzyme Hae III with all kinds of probes dispersed widely from high to low molecular weight of DNA fragements, and Hinf I located only low molecular fragements except M13 probe combination. This research can be concluded restriction enzyme Hae III and Hinf I with M13 and YNZ22 probes will be the best combination of DNA fingerprinting for Korean Cattle.