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18.97.14.81
18.97.14.81
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SCIE SCOPUS
이온교환 크로마토그래피에 의한 Lysozyme 의 추출에 관한 연구
Studies on the Lysozyme Isolation by Ion - exchange Chromatography
이성기(S . K . Lee), 유익종(I . J . Yoo), 민병용(B . Y . Min)
UCI I410-ECN-0102-2008-520-002112455
* This article is free of use.

Various ion-exchange column chromatography for efficient isolation of lysozyme from egg white were investigated. Homogeniged egg white adsorbed through the resin and eluted with 0.5% NaCl. Eluate was precipitated at isoelectric point(pH 9.5) and then dried at 90℃ and freeze dryer. CM Sephadex C-25 was highly efficient in adsorbing lysozyme from egg white. But it has some drawbacks due to volumn change and resin clogging by egg white compared with Duolite C-464. Densitometric peaks on the SDS-PAGE showed high purity of isolated lysozyme using CM Sephadex C-25. The process for lysozyme isolation acheived 95% recovery at Duolite C-464 and 36,000 units/mg activity at CM Sephadex C-25, respectively.

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