These studies was carried out to clarify the genetic relationships of Korean Native Cattle by blood protein polymorphisms. Heterozygosity, genetic identity and genetic distance among five regional groups of Korean Native Cattle were estimated and dendrograms were constructed by principal component analysis and cluster analysis. The genetic differences among regional groups of Korean Native Cattle were not significant but Korean Native Cattle could be classificated in two groups of Seosan-Jeonla-Chungchoung and Kangwon-Kyoungsang by dendrogram. It was found, by dendrogram using data of other reports, that the genetic influence of Indian breed on Korean Native Cattle was slight.