The plasmids of Lactococcus lactis KCTC 2184 producing nisin were studied to find out the nisin plasmid. Lactococcus lactis KCTC 2184 did not ferment lactose and produced raisin, and possessed 9 plasmids and their sizes are 25.1 Mdal, 21.0 Mdal, 17.0 Mdal, 9.2 Mdal. 5.2 Mdal, 3.4 Mdal, 2.7 Mdal, 1.8 Mdal and 1.4 Mdal. Seventeen Mdal plasmid of Lactococcus lactis KCTC 2184 was found to code both raisin production and sucrose-fermenting ability.