This studies were carried out to investigate the extent of actin-myosin dissociation with actin consent observed during myosin Preparation. Actin, tropomyosin and 30,000 dalton components were observed in the myosin sample from the muscle with no electrical stimulation which was stored for 7 days at 0℃ However, these components were not observed in the myosin sample, which was prepared from electrically stimulated muscle stored for 7 days at 0℃