The acyl group compositions of alk-1-enylacyl-, alkylacyl-, and diacyl-from spinal cord of five month old rats were examined. Diacyl-GPC (82.0%) showed the higheest proportion in diradly GPC, and alk-l-enylacyl (59.3%) was highest in diradyl GPE. The major acyl chain of alk-1-enylacyl GYC included C 16:0, C18:1 and C20:1, whereas alk-1-enylacyl GPE was mainly composed of C18:0, C18:1 and C20:1. In alkylacyl group, C 16:0, C 18:1 and C20:1 were main acyl groups in both CGP and EGP, and diacyl GPC showed high proportions of C 16:0, C 18:0 and C 18:1 while its GPE count-part showed high C 18:0, C 18:1 and C20:1 fatty acids. In general, small amounts of C 18:2, C22:4 (n-6), C22:5 (n-6) and C22:5 (n-3) were found in alk-1-enylacyl, alkylacyl and diacyl analogues of GPC and GPE. Lower rate of C18:1 (25.0%) from diacyl GPE was determined in this study than other studies.