Precise analysis of chromosomal genetic materials is not only important at cytogenetics field but basic steps of molecular genetics. To approach these aims through this study, G- and C- banding techniques were used. G- banding was found more accurate patterns at macro-chromosomes compared with recent reported banding patterns. While C- banding was gotten precise heterochromatin site of all chromosomes. So genetic markers dependent on economic traits of chicken will be identified based on advanced banding techniques.