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SCIE SCOPUS
수정란이식에 의한 우 ( 牛 ) 의 쌍태유기에 관한 연구 3 . 수정란의 비외과적 회수
Twin Induction by Embryo Transfer in Cattle 3 . Non - surgical recovery of embryo
정길생 , 이훈택 , 박흠대 , 정병현 , 유승환 ( Kil S . Chung , Hoon T . Lee , Heum D . Park , Byung H . Chung , Seoung H . Yoo )
UCI I410-ECN-0102-2008-520-002140480
* This article is free of use.

These experiments were undertaken to develope a simple and reliable method of nonsurgical ova collection under the field condition. Uterine horns of 37 cows were non-surgically flushed with Dulbecco`s phosphate buffered saline supplemented with 1% fetal calf serum by two different flushing procedures; the gravity and pressure flushing method using two way foley catheter. The results obtained in these experiments were summarized as follows: 1. It was possible to pass the catheter through the cervix to infuse the flushing medium into the uterine cavity in all cows tried, but of 37 cows tried to recover ova, 70.3% succeeded in recovery of 1 or more ova. 2. The highest success rate (87.5%) in ova recovery was obtained from the 1 or 2 calved cows and the lowest (57.1%) from 5 or more calved cows. 3. The recovery rates of flushing medium infused into the uterine cavity by gravity and pressure flushing method were 94.0 and 96.6%, respectively. 4. An average of 4.2 and 4.9 ova per cow were recovered yielding recovery rates of 56.7% and 59.5%, respectively, by gravity and pressure flushing methods. 5. It could be concluded from the above mentioned results that the pressure flushing method is more reliable and effective than the gravity flushing method in saving flushing medium and collecting ova.

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