This experiment was carried out to evaluate the effect of nitrogen fertilization on the rate of growth, and the changes of carbohydrate, nitrate-nitrogen and crude protein content contained in the samples after seedling establishment of orchardgrass. Plants were grown in the pots fertilized light and increasing nitrogen, till dune 13, 1979 after sowing on the, April 12, 1979. The result, obtained were a, follow; 1. Dry matter yield in the pots fertilized increasing nitrogen way more than that in light nitrogen with aging. The liberal use of nitrogen fertilizers after seedling establishment stimulated the growth of orchardgrass in the early growing stage. 2. The convent of total water-soluble carbohydrate, (T. S. C) m stubble, leaves and shoots temporally decreased following nitrogen fertilization after seedling establishment, after which it again increased, and that of plant parts in the pots fertilized increasing nitrogen decreased more than that of plant part; in light nitrogen. 3. Stubble were higher in the content of total water-soluble carbohydrates (T. S. C.) than leaves, and the content of total water-soluble carbohydrate, (T. S. C.) of plant party decreased by tillering. 4. Nitrate-nitrogen content decreased following nitrogen fertilization and gradually increased, however, it was shown again trend, decreased at the end of experiment. Crude protein content rapidly increased immediately following nitrogen fertilization, however, it gradually decreased.