This experiment was carried nut to investigate the nutritive value of antibiotic fermentation by-product (Ugifac) for broiler diets, 270 birds of Hybro male chicks were divided into 6 groups, which were the substitution level by Ugifac 0, 1, 2, 3, 4, 5% for 8 weeks. The results obtained were as follows; 1. Ugifac contains 68.60% C. Ash, 17.32% C. Protein, and 0.67㎎/g of kanamycin. 2. By increasing the levels of Ugifac, it was tended to increase body weight gain and the amounts of feed consumption. 4% levels of Ugifac was better than any other treatments in terms of feed efficiency and economics. 3. There was no evidence of antibiotics retention on muscle and liver of chicks although Ugifac was supplemented till 5%. In conclusion Ugifac can be used within the 4%. levels for broiler diets efficiently without antibiotics retention problems, although the chemical composition of Ugifac was relatively low in quality.