This experiment was conducted to compare the effects of glutamic acid fermentation residue (G.A.F.R) and urea as a source of nitrogen on the growth, production and chemical composition of pasture species over a period of year from Sept. 7, 1977 to Sept. 24, 1978. Five different fertilizing rates of urea and G.A.F.R arranged according to the amount of nitrogen; 0, 15 ㎏ N/l0a as urea 9, 15 and 21 ㎏ N / l0a as G.A.F.R. The results obtained are summarized as follows: 1. The seedling establishment of pasture species was greatly depressed by urea and G.A.F.R fertilization. As the amount of G.A.F.R fertilizing rate increased the seedling establishment of pasture species depressed proportionately. 2 . Winter hardiness of pasture species decreased when the fertilizing rate of G.A.F.R. increased and urea was a little more effective than G.A.F.R at the same amount of nitrogen except for Italian ryegrass. 3. Dry matter yield of pasture species per l0a was increased significantly with urea and G.A.F.R. (p$lt;0.01) 4. The botanical composition of grasses was increased with urea and G.A.F.R, while ladino clover was decreased 5. The crude protein content of pasture species was increased markedly with urea, and G.A.F.R, while nitrogen free extract was decreased. 6. The results obtained from this experiment indicated that G.A.F.R, as a nitrogen source was equally or more effective on the growth, production and chemical composition of pasture species compared with urea.