To know the physico-chemical properties of Saanens milk, this experiment has been carried out with Saanens milk collected from Wonju, Kangwon province. Main components and physico-chemical properties of Saanens milk were analyzed. Skim milk, acid caseins and whey proteins obtained from Saanens milk were fractionatd on DEAE-cellulose column. The results were as follows; 1. Total solids, milk fat, proteins, lactose, and ash of Saanens milk were 10.12-13.6% (average 11.61%), 2.3-4.7%(average 3.27%), 3.09-4.36%(average 3.65%), 3.79-4.03%(average 3.91%), and 0.62-0.86%(average 0.78%), respectively. 2. Physico-chemical properties of Saanens milk were 1.026-1.033(average 1.031) in specific gravity, 6.504-7.055(average 6.734) in pH, 0.117-0.193%(average 0.157%) in acidity, positive in alcohol test, and negative in boiling test. 3. Casein percentage, whey protein, lactoglebulin, lactalbumin, soluble nitrogen compounds at 12% TCA(total nitrogen percentage), nitrogen compounds precipitated at 12% TCA (total nitrogen percentage), soluble nitrogen compounds at 3% TCA(total nitrogen percent age), nitrogen compounds precipitated at 3% TCA(total nitrogen percentage) were 1.944%, 1.202 0.156%, 1.046%, 19.31%, 80.69%, 21.36%, and 78.64% respectively. 4. on DEAE-cellulose column, Saanens skim milk was fractionated into 5 fractions, acid casein was fractionated into 4 fractions, and whey protein was fractionated into 5 fractions.