Two Anatoliian queens imported from Canada were crossed with Italian drones. The first cross queens were also backcrossed with Italian drones. Italian colonies used were kept to be almost twice stronger than those of the first cross or back cross throughout the year. The first cross produced significantly (P$lt;0.05) more honey than Italian or back cross on the bee pastures such as acacia, chestnut and lespedeza except for rape. However, there was no significant (P$lt;0.05) difference between Italian and back cross in the honey yield. The heterotic effect by the first cross over Italian for the yearly honey yield per 10,000 bees was 1.2㎏., which amounted to about 70% of the total yield by the same number of Italian bees.