This experiment was conducted to induce the superovulation of the rabbits. Sixteen mature rabbits weighing 2 ㎏ on the average were fed preliminarily for a month. The treatments were done as below; 1. Estrogen (4000IU)+P.M.S. (250IU)×5 days+H.C.G.(125IU) and mating. 2. P.M.S. (50IU)×5 days+Estrogen(2000IU)+H.C.G. (250IU) and mating. 3. P.M.S. (50IU)×5 days+H.C.G. (250IU) and mating. The results obtained are as follows: 1. The group injected intramusculary 250 IU of P.M.S. showed no superovulation, and superovulation was occurred in the group injected subcutaneously 50 IU of P.M.S., the ovulation rate bring 4 to 5 times than the normal rabbits. 2. Estrogen treatment was not significant for the superovulation of the rabbits, but increased the mating capacity and enlarged the follicles and blood follicle.