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카드늄을 처리한 미나리 (Oenanthe javanica)에서 전체 Thiol 잔기 , 글루타치온 , Phytochelatin의 농도 변화
Effect of Cadmium Treatment on the Total Thiol Groups , Glutathione and Phytochelatin Contents in Oenanthe javanica
민복기 , 최순용 , 이세영 ( Bokkee Min , Soon Yong Choi , Se Yong Lee )
UCI I410-ECN-0102-2008-520-000727810

In order to evaluate the usefulness of O. javanica for the phytoremediation, it was grown for 1, 3, 7, 14, 21 days and was exposed to 50 μM of CdCl₂ in hydroponic medium after 3 weeks. Its biomass and contents of chlorophylls were analyzed. The growth of O. javanica showed little difference between cadmium treated and non-treated groups, while its contents of chlorophylls of Cd-treated group decreased up to 50% compared to the case of non-treated group. Its accumulated cadmium concentrations were 2.1, 7.3 and 113 μmoles Cd/g dry weight in the leaf, stem and root, respectively. The total contents of thiol increased 0.5, 1 and 7 times in the leaf, stem and root, respectively, while the contents of glutathione tended to decrease by 43%, 70% and 47% in the leaf, stem and root, respectively. Using HPLC analysis, the reasonable peaks of thiol compounds in shoot and root of Cd-treated sample were compared to those of non-treated sample in O. javanica, and found to be phytochelatins. In case of Nicotiana tabacum cv. Xanthi tested as control plant, the cadmium treatment for 3 weeks resulted in the decrease of both biomass and chlorophyll up to 70% and 75%, respectively. The roots of tobacco became rotten and eventually died. These results suggested that Oenanthe javanica is cadmium-tolerant hyperaccumulator.

[자료제공 : 네이버학술정보]
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