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젓갈에서 분리한 Lactobacillus spp . 로 제조한 요구르트의 이화학적 특성
Physico-chemical Characteristics of Yogurt by Lactobacillus spp . from Picklee
김종현(Jong Hyun Kim), 이영환(Young Hwan Rhee), 나한주(Han Ju Na), 이용규(Yong Kyu Lee), 신승이(Seung Yee Shin)
UCI I410-ECN-0102-2008-520-000729410

Three strains of lactic acid bacteria were isolated from fish and shrimp pickles. Two strains were identified as Lactobacillus casei, and one strain as L. pentosus, respectively. All three strains were used as starters in producing yogurts. The physico-chemical characteristics of yogurts were examined. The original pH, titratable acidity, visicosity and viable cell counts of the yogurts were 4.03∼4.26, 1.049∼1.217%, 1,772∼2,232 cps and 1.4×10^9∼1.6×10^9 cfu/㎖, respectively. In evaluating buffer capacity, 12.50∼14.06 ㎖ 1.0N HCl was consumed to titrate 100 ㎖ of yogurt to pH value 2 units below the original pH value and 9.46∼13.06 ㎖ of 1.0N NaOH was consumed to pH value 4 units above the original pH value. The β-glactosidase activity reached maximum at 48 hrs, and reduced gradually during fermantation. After 2 hr incubation of yogurts at 37℃ under different pH conditions, β-glactosidase activities of three strains were reduced to 50% at pH 3.5, but there were no remaining activities neither at pH 2.5 nor at pH 1.5. Under the same pH conditions the number of viable cells decreased to 1.9×10^6∼1.8×10^8 cfu/㎖ at pH 2.5 and 1.0×10³∼2.4×10^5 at pH 1.5, respectively. However, no significant difference was found at pH 3.5.

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