In the course of searching for new insecticidal compounds from soil microorganisms, compound 3369 was isolated and identified as oligomycin A, which is known as a potent inhibitor of mitochondrial ATPase. We tried to change the structure chemically in order to improve its toxicity (LD_(50) 2.5㎎/㎏ mouse) and insecticidal activity and got compound 3369SM1 and 3369N. These changed compounds showed decreased insecticidal activities and showed newly anti microbial activities. From these results, we deduced that changed sites of the compound 3369 structure may be essential for the insecticidal activity and these changed compounds may have new bioactivities.