To understand the molecular structure and pathogenesis mechanism of garlic latent virus (GLV), it was purified by serial infection in Vicia faba which shows local necrotic spot by inoculation and amplified in Allium porrum L. which was considered to be a systemic host for GLV. Particle length of garlic viruses from mixed infected garlic plants ranged from 200 ㎚ to 2000 ㎚ by electron microscopy, but most of the particle was in the range of 600∼900 ㎚. Purified GLV particles showed 690 ㎚ long in average and their shape was slightly curved filamentous rod. Structural protein of garlic virus isolated from mixed-infected garlic leaves distributed between the range of MW 24∼38 kDa but GLV coat protein was 34 kDa. In the previous experiment, we have isolated several cDNA clones for garlic virus and classified these clones into 4 different groups on the basis of cross Southern hybridization. To identify cDNA clone for GLV, Northern blot analysis was carried out with 4 different cDNA clones as probes. The result showed that clone 28∼29 is a partial cDNA clone for GLV. The result of Northern blot analysis demonstrated that genome size of GLV is 8.2 kb and had poly (A) tail. Nucleotide sequence shows that a partial cDNA clones, 28∼9 and G8, have the length of 2140 bp.