The present study was carried out to investigate the effect of Ni on germination, cell elongation, α-amylase activity, contents of chlorophyll and protein in radish were determined in the water culture. As the concentration of Ni was increased in the water culture, germination of radish was 55% by Ni 10 ㎎/㎏ and 30c7c by Ni 20 ㎎/㎏. The ratio of cell elongation injury was 50% by two days after Ni 20 ㎎/㎏ treatment. The injury ratio of α-amylase activity was 45% in the same condition and as the time goes on, inhibition of α-amylase activity were slightly decreased. Contents of chlorophyll a and b were decreased two days after treatment and chlorophyll a was more inhibited than chlorophyll b. Also changes of the protein contents was slightly decreased. Activity of α-amylase was decreased at germination stage, contents of chlorophyll a and b were decreased at growing stage.