Barbaloin in the Aloe vera depending on the various extracting conditions was analyzed by HPLC. The contents of the barbaloin extracted by the solvents increased in the order of methanol$gt;ethanol$gt;water extraction. In setting extraction, the contents of barbaloin extracted with methanol and ethanol were increased from four hours at 60℃ and then decreased. The contents of barbaloin extracted with water were different depending on the temperature and time. Increasing the extracting time and temperature, the contents of barbaloin were decreased in water extract. It was estimated that the barbaloin might be stable in organic solvent, but decomposed with hydrolysis in water.